scholarly journals Immunofluorescence Detection of F-actin on Low Melting Point Wax Sections from Plant Tissues

1997 ◽  
Vol 45 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Stanislav Vitha ◽  
František Baluška ◽  
Miriam Mews ◽  
Dieter Volkmann
Keyword(s):  
Melting Point ◽  
Developmental Stages ◽  
Lepidium Sativum ◽  
Plant Tissues ◽  
Root Tips ◽  
Reliable Technique ◽  
Large Numbers ◽  
Indirect Immunofluorescence Technique ◽  
Pass Through ◽  
First Time

We developed a simple and reliable technique for immunofluorescence detection of F-actin on microtome sections of plant tissues. For the first time, large numbers of plant cells from various tissues that pass through their developmental stages could be consistently visualized on one section from plant organs. n-Maleimidobenzoic acid N-hydroxy-succinimide ester-pretreated and formalin-fixed segments of plant roots and shoots were embedded in low melting point ester wax at 37C and sectioned on a microtome. After dewaxing and rehydration, microfilaments were visualized by indirect immunofluorescence technique with a monoclonal anti-actin antibody. The technique has been successfully used for visualization of tissue- and development-specific F-actin arrays in cells of Zea mays and Lepidium sativum root tips and of maize stem nodes.

Localization of acid phosphatases in root cap of rice plant

1991 ◽  
Vol 49 ◽  
pp. 224-225
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
W. S. Chen
Keyword(s):  
Rice Plant ◽  
Developmental Stages ◽  
Uranyl Acetate ◽  
Root Cap ◽  
Plant Tissues ◽  
Acid Phosphatases ◽  
Root Tips ◽  
Buffer Solution ◽  
Cacodylate Buffer ◽  
Ethanol Series

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.

scholarly journals A new species of Micrurapteryx (Lepidoptera, Gracillariidae) feeding on Thermopsis lanceolata (Fabaceae) in southern Siberia and its hymenopterous parasitoids

ZooKeys ◽  
2021 ◽  
Vol 1061 ◽  
pp. 131-163
Author(s):  
Natalia I. Kirichenko ◽  
Evgeny N. Akulov ◽  
Paolo Triberti ◽  
Sergey A. Belokobylskij
Keyword(s):  
New Species ◽  
Developmental Stages ◽  
Dna Barcode ◽  
Reference Sequence ◽  
Large Numbers ◽  
Leaf Mining ◽  
Morphological Diagnosis ◽  
The Republic ◽  
First Time ◽  
A New Species

A new species of leaf-mining moth described here as Micrurapteryx baranchikovi Kirichenko, Akulov & Triberti, sp. nov. was detected in large numbers feeding on Thermopsis lanceolata (Fabaceae) in the Republic of Khakassia (Russia) in 2020. A morphological diagnosis of adults, bionomics and DNA barcoding data of the new species are provided. The developmental stages (larva, pupa, adult), male and female genitalia, as well as the leaf mines and the infestation plot in Khakassia are illustrated; the pest status of the new species in the studied region is discussed. Additionally, parasitism rate was estimated, the parasitoid wasps reared from pupae of the new species were identified (morphologically and genetically) and illustrated . Among them, one ichneumonid, Campoplex sp. aff. borealis (Zetterstedt) and two braconids, Agathis fuscipennis (Zetterstedt) and Illidops subversor (Tobias et Kotenko), are novel records for the Republic of Khakassia. Furthermore, they are all documented as parasitoids of Gracillariidae for the first time. The DNA barcode of A. fuscipennis is newly obtained and can be used as a reference sequence for species identification.

scholarly journals Evolutionary and Expression Analysis of CAMTA Gene Family in Three Species (Arabidopsis, Maize and Tomato), and Gene Expression in Response to Developmental Stages

2021 ◽  
Author(s):  
Abbas Saidi ◽  
Zohreh Hajibarat
Keyword(s):  
Stress Proteins ◽  
Developmental Stages ◽  
Plant Tissues ◽  
Expression Data ◽  
Calmodulin Binding ◽  
Percent Identity ◽  
Genome Wide ◽  
Mrna Analysis ◽  
Gene Structures ◽  
First Time

Abstract The calmodulin-binding transcriptional activator (CAMTA) family has been known to be one of the fast responsive stress proteins. In this study, 17 CAMTA genes were selected in Arabidopsis, tomato and maize. The chromosomal distributions, gene structures, duplication patterns, phylogenetic tree, and developmental stage of the 17 CAMTA genes in the three species were analyzed to further investigate their functions. According to the synteny analysis, CAMTA genes of maize and tomato revealed higher similarity with each other as compared with Arabidopsis. A higher than 90 percent identity was observed between maize CAMTA genes (ZmCAMTA2 and ZmCAMTA3) and tomato CAMTA genes (SlCAMTA4, SlCAMTA4.1). To detect expression levels in different plant tissues, mRNA analysis of CAMTA genes were performed using publicly available expression data in the genvestigator. The aim of study was to identify and characterize CAMTA genes in three species, for the first time, via insilico genome-wide analysis approach. AtCAMTA1 and AtCAMTA2 and SlCAMTA2 and ZmCAMTA1 and ZmCAMTA2 genes were up-regulated during all developmental stages. The conserved motifs and gene structure in most proteins in each group were similar, validating the CAMTA phylogenetic classification. This study could be considered as a useful source for future CAMTA comparative studies in different plant species.

scholarly journals Probing the floral developmental stages, bisexuality and sex reversions in castor (Ricinus communis L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sujatha Thankeswaran Parvathy ◽  
Amala Joseph Prabakaran ◽  
Thadakamalla Jayakrishna
Keyword(s):  
Developmental Stages ◽  
Ricinus Communis ◽  
Sex Expression ◽  
Pistil Abortion ◽  
Ricinus Communis L ◽  
Sex Reversion ◽  
Stage 4 ◽  
Meristem Development ◽  
Male Specific ◽  
First Time

AbstractCastor (Ricinus communis L) is an ideal model species for sex mechanism studies in monoecious angiosperms, due to wide variations in sex expression. Sex reversion to monoecy in pistillate lines, along with labile sex expression, negatively influences hybrid seed purity. The study focuses on understanding the mechanisms of unisexual flower development, sex reversions and sex variations in castor, using various genotypes with distinct sex expression pattern. Male and female flowers had 8 and 12 developmental stages respectively, were morphologically similar till stage 4, with an intermediate bisexual state and were intermediate between type 1 and type 2 flowers. Pistil abortion was earlier than stamen inhibition. Sex alterations occurred at floral and inflorescence level. While sex-reversion was unidirectional towards maleness via bisexual stage, at high day temperatures (Tmax > 38 °C), femaleness was restored with subsequent drop in temperatures. Temperature existing for 2–3 weeks during floral meristem development, influences sexuality of the flower. We report for first time that unisexuality is preceded by bisexuality in castor flowers which alters with genotype and temperature, and sex reversions as well as high sexual polymorphisms in castor are due to alterations in floral developmental pathways. Differentially expressed (male-abundant or male-specific) genes Short chain dehydrogenase reductase 2a (SDR) and WUSCHEL are possibly involved in sex determination of castor.

scholarly journals First Report of a Gall Midge Species (Diptera: Cecidomyiidae) Associated With Pistachios

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Farshid O Sirjani ◽  
Edwin E Lewis
Keyword(s):  
Gall Midge ◽  
Species Level ◽  
Plant Tissues ◽  
First Report ◽  
Branch Dieback ◽  
First Time ◽  
Level Identification

Abstract A new dipterous pest is reported, for the first time, on commercial pistachios from Sirjan, Kerman province, Iran. The genus of the insect was determined to be Resseliella Seitner (Diptera: Cecidomyiidae). Adults are light brown to brown in color and 0.8–1.5 mm in length with females, generally, slightly larger than males. Females have an elongated ovipositor, which is characteristic of the genus. Larvae are orange in color, 2–3 mm in length in the later instars, feed under bark without inducing galls, and cause branch dieback on trees of various ages. Brown to black discolorations are observed on plant tissues under bark where the larvae feed. Infestations observed on current and the previous—year’s growths, ranged from 0.5 to 1.2 cm in diameter, and all located in outer branches. Dry leaves and fruit clusters on infested branches remain attached, which may be used to recognize infestation by the gall midge. Dark-colored, sunken spots with splits on the bark located at the base of the wilted sections of the shoots also are symptoms of Resseliella sp. larval activity. Species-level identification of the gall midge is currently underway.

scholarly journals Combining QTL Mapping and Gene Expression Analysis to Elucidate the Genetic Control of ‘Crumbly’ Fruit in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 794
Author(s):  
Luca M. Scolari ◽  
Robert D. Hancock ◽  
Pete E. Hedley ◽  
Jenny Morris ◽  
Kay Smith ◽  
...  
Keyword(s):  
Genetic Control ◽  
Developmental Disorder ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Microarray Experiment ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Genotype By Sequencing ◽  
First Time ◽  
Selection Of

‘Crumbly’ fruit is a developmental disorder in raspberry that results in malformed and unsaleable fruits. For the first time, we define two distinct crumbly phenotypes as part of this work. A consistent crumbly fruit phenotype affecting the majority of fruits every season, which we refer to as crumbly fruit disorder (CFD) and a second phenotype where symptoms vary across seasons as malformed fruit disorder (MFD). Here, segregation of crumbly fruit of the MFD phenotype was examined in a full-sib family and three QTL (Quantitative Trait Loci) were identified on a high density GbS (Genotype by Sequencing) linkage map. This included a new QTL and more accurate location of two previously identified QTLs. A microarray experiment using normal and crumbly fruit at three different developmental stages identified several genes that were differentially expressed between the crumbly and non-crumbly phenotypes within the three QTL. Analysis of gene function highlighted the importance of processes that compromise ovule fertilization as triggers of crumbly fruit. These candidate genes provided insights regarding the molecular mechanisms involved in the genetic control of crumbly fruit in red raspberry. This study will contribute to new breeding strategies and diagnostics through the selection of molecular markers associated with the crumbly trait.

The habits and habitats of the opisthobranch molluscs of the British Solomon Islands

1969 ◽  
Vol 255 (800) ◽  
pp. 541-548 ◽  
Keyword(s):  
Natural History ◽  
Solomon Islands ◽  
Gut Contents ◽  
Numerical Evidence ◽  
Large Numbers ◽  
Different Types ◽  
Opisthobranch Molluscs ◽  
First Time ◽  
Reef Formation

Often bizarre in shape and gaudily coloured, the opisthobranchs of the tropical IndoPacific have always been attractive subjects for the specialist worker and for writers of popular natural history. There is certainly no lack of papers describing their anatomy and systematics (Marcus & Burch (1965) give a fairly full bibliography), but only in those by Risbec (1928 a, b , 1951, 1953) are there descriptions of where or how they live and his statements are brief and rather vague. I collected opisthobranchs so as to record them for the British Solomon Islands for the first time and, whenever possible, I noted their habits and habitats. My observations on gut contents are rather scanty as I did not wish to damage any of the few specimens of each species collected. From an examination of ten different types of reef formation I discovered that opisthobranchs are common in only two places; (1) the crest of a semi-exposed reef where algae occur in pools, and (2) the flat of a sheltered or semi-sheltered reef. At each of these sites several distinct habitats can be distinguished. Unfortunately my recognition of these habitats is not supported by strong numerical evidence as none of the 55 species occurred in large numbers.

Low energy electron ranges in liquids: determination by nonhomogeneous kinetics

1977 ◽  
Vol 55 (11) ◽  
pp. 2050-2062 ◽  
Author(s):  
J.-P. Dodelet
Keyword(s):  
Critical Temperature ◽  
Melting Point ◽  
Distribution Functions ◽  
Liquid Density ◽  
Range Distribution ◽  
Total Ionization ◽  
Free Ion ◽  
Drastic Increase ◽  
Pass Through ◽  
Ion Yields

Free ion yields have been measured in four hydrocarbon liquids: n-pentane, cyclopentane, neopentane, and neohexane. Each liquid has been studied from room temperature or below up to the critical temperature. Theoretical curves have been calculated using the relation between the free ion yields and the external field strength derived by Terlecki and Fiutak on the basis of an equation by Onsager. Two popular electron range distribution functions, Gaussian and exponential, have been shown not to be an adequate representation of the reality as far as the model used for the calculations is concerned. In order to fit experimental points, both range distribution functions would require a drastic increase in the total ionization yield, Gtot, with temperature increase. This would mean an unrealistic decrease of the ionization potential of the molecule from the melting point up to the critical temperature.It is possible to keep Gtot quite constant and within the range of values obtained by other techniques by extending the Gaussian range distribution function with a (range)−3 probability tail. The most probable range can be normalized for the liquid density. This parameter has been used to obtain information about the behaviour of epithermal electrons in the four alkane liquids from the melting point up to the critical temperature.(1) Normalized penetration ranges of epithermal electrons are dependent on the structure of the molecule in the entire liquid range but differences are smaller at critical than at low temperatures.(2) Normalized penetration ranges of epithermal electrons pass through a maximum in the liquid phase for neopentane, neohexane, and cyclopentane. No maximum is observed for n-pentane.(3) There is no drastic change in the behaviour of epithermal electrons in these alkanes at the critical temperature.

scholarly journals Evaluation of Effective and Practical Euthanasia Methods for Larval African Clawed Frogs (Xenopus laevis)

2020 ◽  
Vol 59 (3) ◽  
pp. 269-274
Author(s):  
Ilana A Galex ◽  
Cameron M Gallant ◽  
Nicole D'Avignon ◽  
Lauren M Kuchenbrod ◽  
Craig A Fletcher ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Developmental Stages ◽  
Age Group ◽  
Research Model ◽  
Larval Stages ◽  
Large Numbers ◽  
Guidance Documents ◽  
Stock Solutions ◽  
Tricaine Methanesulfonate ◽  
Tank Water

Larval, or tadpole-stage Xenopus laevis frogs are a popular research model for developmental biology and disease studies. Existing euthanasia guidance documents offer recommendations for both eggs and adult stages, yet do not specifically address the larval stage. Data evaluating effective euthanasia methods for groups of X. laevis tadpoles would therefore be useful. The goal of the current study was to evaluate the efficacy of various immersion euthanasia procedures on tadpoles: tricaine methanesulfonate (MS222) at 6 g/L, eugenol at 800 μL/L and rapid chilling (2 to 4 °C). We also evaluated tadpoles at various developmental stages (NF stages 46, 47 and 49). Tadpoles (n = 70) were exposed to euthanasia solution for 15 min, and controls (n = 40) were placed in housing tank water for 15 min. All animals were then placed in recovery tanks containing housing tank water for 4 h to confirm irreversibility of each agent. Cessation of the heartbeat was assessed at the end of euthanasia solution exposure and at each hour thereafter. We found that immersion in a 6 g/L solution of MS222 resulted in 100% euthanasia of all larval stages tested. Conversely, eugenol produced variable euthanasia rates that were affected by both age group and batches of stock solutions. Rapid chilling was completely ineffective as a euthanasia method in our study. Based on our findings, we recommend MS222 as an effective and practical means of euthanizing large numbers of X. laevis tadpoles.

scholarly journals Rigid ventilating video bronchoscope with forceps for bronchoscopic foreign body removal in children

2019 ◽  
Vol 5 (4) ◽  
pp. 1129
Author(s):  
Sagar Jawale ◽  
Parthapratim Gupta ◽  
Bharti Kulkarni
Keyword(s):  
Foreign Body ◽  
Gold Standard ◽  
Medical Literature ◽  
Rigid Bronchoscopy ◽  
Foreign Body Aspiration ◽  
Indian Children ◽  
Foreign Body Removal ◽  
Rigid Tube ◽  
Pass Through ◽  
First Time

<p>Bronchoscopic foreign body removal is a potentially dangerous and challenging procedure in pediatric surgery. bronchoscopy under general anaesthesia is the gold standard of diagnosis and management of foreign body aspiration. A large ventilating channel and better control over the tip of the instrument and cheaper instrument are the merits of rigid bronchoscopy over flexible one. Traditionally a rigid tube alone is used for this purpose which has extreme limitations of vision and it is risky. Foreign body aspiration typically occurs in 6 to 18 month age and the size of glottis is very small at this age. In Indian children who are small and malnourished the large assembly of sheath and telescope mounted forceps does not pass through the glottis. To overcome the limitations of the traditional equipment I designed my own bronchoscopy equipment by my 15 year of experience in bronchoscopy. This type of device is reported for the first time in medical literature and patent is filed for it at Mumbai office.</p><p> </p>

Sign in / Sign up

Export Citation Format

Share Document