Topical Administration of Tetrasodium-Mesotetraphenyl-Porphinesulfonate (TPPS) and Red Light Irradiation for the Treatment of Superficial Neoplastic Lesions

1987 ◽  
Vol 73 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Virgilio Sacchini ◽  
Elsa Melloni ◽  
Renato Marchesini ◽  
Tommaso Fabrizio ◽  
Natale Cascinelli ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Complete Remission ◽  
Treatment Effectiveness ◽  
Red Light ◽  
Topical Administration ◽  
Percutaneous Penetration ◽  
Drug Penetration ◽  
Photosensitizing Agents ◽  
Neoplastic Lesions ◽  
Percutaneous Penetration Enhancer

In photodynamic therapy, systemic administration of photosensitizing agents induces cutaneous photosensitization in patients. This side-effect can be avoided by topical administration of the agents when only surface lesions are involved. A hydroalcoholic solution of tetrasodium-meso-tetraphenylporphinesulfonate (TPPS) containing Azone, a percutaneous penetration enhancer, was investigated to evaluate its photosensitizing potential in the treatment of 33 primary and recurrent neoplastic lesions of the skin. A complete remission was obtained of lesions with clinical thickness of less than 2 nun. Treatment effectiveness depends on both light and drug penetration through skin. Further studies are in progress to optimize treatment parameters.

Topical Administration of Tetrasodium-Mesotetraphenyl-Porphinesulfonate (TPPS): Correlation between Drug Penetration and Depth of Necrosis in Skin of Nude Mice following Red Light Irradiation

1987 ◽  
Vol 73 (1) ◽  
pp. 11-17 ◽  
Author(s):  
Renato Marchesini ◽  
Elsa Melloni ◽  
Giovanni Bottiroli ◽  
Salvatore Andreola ◽  
Giannino Fava ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Nude Mice ◽  
Red Light ◽  
Local Treatment ◽  
Topical Administration ◽  
Skin Lesions ◽  
Drug Penetration ◽  
Suitable Treatment ◽  
Epithelial Layers ◽  
Photosensitizing Agent

The main side effect in photodynamic therapy is photosensitization of the patient's skin following systemic administration of the photosensitizing agent. In the case of superficial lesions, this problem can be avoided by topically applying the drug: in this way a local treatment can be performed. We tested the photosensitizing properties of a 2 % solution of TPPS (tetrasodium-tetraphenylporphinesulfonate) in a vehicle containing a penetration enhancer, Azone, on skin of nude mice. An aliquot of 0.1 ml/cm2 of the solution was painted on the skin overlying an s.c. implanted NMU-1 tumor. Subsequently, animals were sacrificed at different times after application. Fluorescence microscopy revealed that TPPS penetration depth was related to time elapsed after application and to painting modalities. Solution penetration was enhanced by wiping with ether immediately before painting. Irradiation at 80 mW/cm2 for 20 min with a dye laser emitting at 640 am, 4 h after TPPS application, produced necrosis of the upper skin layers, up to 0.2 mm in depth. These findings suggest that topical TPPS administration, followed by laser irradiation, may be a suitable treatment modality for skin lesions involving epithelial layers, even though several aspects of this metodology need further investigation.

Use of photodynamic therapy for photodamage skin (review of literature)

2019 ◽  
Vol 1 (7) ◽  
pp. 19-23
Author(s):  
S. I. Surkichin ◽  
N. V. Gryazeva ◽  
L. S. Kholupova ◽  
N. V. Bochkova
Keyword(s):  
Photodynamic Therapy ◽  
Comparative Evaluation ◽  
Clinical Manifestations ◽  
Light Sources ◽  
Review Of Literature ◽  
Photosensitizing Agents ◽  
Selection Of

The article provides an overview of the use of photodynamic therapy for photodamage of the skin. The causes, pathogenesis and clinical manifestations of skin photodamage are considered. The definition, principle of action of photodynamic therapy, including the sources of light used, the classification of photosensitizers and their main characteristics are given. Analyzed studies that show the effectiveness and comparative evaluation in the selection of various light sources and photosensitizing agents for photodynamic therapy in patients with clinical manifestations of photodamage.

scholarly journals Liposome Photosensitizer Formulations for Effective Cancer Photodynamic Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1345
Author(s):  
Sherif Ashraf Fahmy ◽  
Hassan Mohamed El-Said Azzazy ◽  
Jens Schaefer
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Adverse Effects ◽  
Cellular Uptake ◽  
Treatment Effectiveness ◽  
State Of The Art ◽  
Oxygen Species ◽  
Chemotherapeutic Drugs ◽  
Invasive Strategy ◽  
Non Invasive

Photodynamic therapy (PDT) is a promising non-invasive strategy in the fight against that which circumvents the systemic toxic effects of chemotherapeutics. It relies on photosensitizers (PSs), which are photoactivated by light irradiation and interaction with molecular oxygen. This generates highly reactive oxygen species (such as 1O2, H2O2, O2, ·OH), which kill cancer cells by necrosis or apoptosis. Despite the promising effects of PDT in cancer treatment, it still suffers from several shortcomings, such as poor biodistribution of hydrophobic PSs, low cellular uptake, and low efficacy in treating bulky or deep tumors. Hence, various nanoplatforms have been developed to increase PDT treatment effectiveness and minimize off-target adverse effects. Liposomes showed great potential in accommodating different PSs, chemotherapeutic drugs, and other therapeutically active molecules. Here, we review the state-of-the-art in encapsulating PSs alone or combined with other chemotherapeutic drugs into liposomes for effective tumor PDT.

scholarly journals Treatment of Recalcitrant Viral Warts with Photodynamic Therapy with Mal and Red Light

2013 ◽  
Vol 03 (01) ◽  
pp. 117-120
Author(s):  
Montserrat Fernández Guarino ◽  
Antonio Harto ◽  
Pedro Jaén
Keyword(s):  
Photodynamic Therapy ◽  
Red Light

scholarly journals Bis[Pyrrolyl Ru(II)] Triads: a New Class of Photosensitizers for Metal-Organic Photodynamic Therapy

2020 ◽  
Author(s):  
Deborah A. Smithen ◽  
Susan Monro ◽  
Mitch Pinto ◽  
John A. Roque III ◽  
Roberto M. Diaz-Rodriguez ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Visible Light ◽  
Red Light ◽  
Visible Region ◽  
Hl60 Cell ◽  
Quantum Yields ◽  
Human Leukemia ◽  
New Family ◽  
Hl60 Cell Line

A new family of ten dinuclear Ru(II) complexes based on the bis[pyrrolyl Ru(II)] triad scaffold, where two Ru(bpy)<sub>2</sub> centers are separated by a variety of organic linkers, was prepared to evaluate the influence of the organic chromophore on the spectroscopic and in vitro photodynamic therapy (PDT) properties of the compounds. The bis[pyrrolyl Ru(II)] triads absorbed strongly throughout the visible region, with several members having molar extinction coefficients (e) ≥10<sup>4</sup> at 600–620 nm and longer. Phosphorescence quantum yields were generally less than 0.1% and in some cases undetectable. The singlet oxygen quantum yields ranged from 5% to 77% and generally correlated with their photocytotoxicities toward human leukemia (HL-60) cells regardless of the wavelength of light used. Dark cytotoxicities varied ten-fold, with EC<sub>50</sub> values in the range of 10–100 µM and phototherapeutic indices (PIs) as large as 5,400 and 260 with broadband visible (28 J cm<sup>-2</sup>, 7.8 mW cm<sup>-2</sup>) and 625-nm red (100 J cm<sup>-2</sup>, 42 mW cm<sup>-2</sup>) light, respectively. The bis[pyrrolyl Ru(II)] triad with a pyrenyl linker (5h) was especially potent, with an EC50 value of 1 nM and PI >27,000 with visible light and subnanomolar activity with 625-nm light (100 J cm<sup>-2</sup>, 28 mW cm<sup>-2</sup>). The lead compound 5h was also tested in a tumor spheroid assay using the HL60 cell line and exhibited greater photocytotoxcicity in this more resistant model (EC<sub>50</sub>=60 nM and PI>1,200 with 625-nm light) despite a lower dark cytotoxicity. The in vitro PDT effects of 5h extended to bacteria, where submicromolar EC<sub>50</sub> values and PIs >300 against <i>S. mutans</i> and <i>S. aureus </i>were obtained with visible light. This activity was attenuated with 625-nm red light, but PIs were still near 50. The ligand-localized <sup>3</sup>ππ* state contributed by the pyrenyl linker of 5h likely plays a key role in its phototoxic effects toward cancer cells and bacteria.<br><br>

scholarly journals Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model

2019 ◽  
Vol 20 (5) ◽  
pp. 1084 ◽  
Author(s):  
Hilde Buzzá ◽  
Lucas Fialho de Freitas ◽  
Lilian Moriyama ◽  
Ramon Teixeira Rosa ◽  
Vanderlei Bagnato ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Molecular Oxygen ◽  
Chorioallantoic Membrane ◽  
Topical Administration ◽  
Inhibitory Effect ◽  
Vascular Effects ◽  
Vascular Effect ◽  
Chick Chorioallantoic Membrane ◽  
Anti Oxidant ◽  
Total Fluence

Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.

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