Protective effects of dietary omega-3 fatty acid supplementation on organophosphate poisoning

2017 ◽  
Vol 34 (2) ◽  
pp. 69-82 ◽  
Author(s):  
Bahattin Avci ◽  
S. Sirri Bilge ◽  
Gokhan Arslan ◽  
Omer Alici ◽  
Ozge Darakci ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
The Body ◽  
Control Group ◽  
Organophosphate Poisoning ◽  
Protective Effects ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Omega 3 Fatty Acid ◽  
Locomotor Activities

In this study, we aimed to study the possible preventive effect of docosahexaenoic acid (DHA), a dietary omega-3 fatty acid, on toxicity caused by chlorpyrifos (CPF). Six groups of Sprague Dawley rats (200–250 g) consisting of equal numbers of males and females (n = 8) were assigned to study. The rats were orally given for 5 days. The control group was administered pure olive oil, which was the vehicle for CPF. The CPF challenge groups were administered oral physiological saline, pure olive oil, or DHA (50, 100 and 400 mg/kg dosages) for 5 days. The animals were weighed on the sixth day and then administered CPF (279 mg/kg, subcutaneously). The rats were weighed again 24 h following CPF administration. The body temperatures and locomotor activities of the rats were also measured. Blood samples, brain and liver tissues were collected for biochemical, histopathological and immunohistochemical examinations. A comparison with the control group demonstrated that CPF administration increased malondialdehyde (MDA) levels in blood, brain and liver, while it reduced catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations ( p < 0.05–0.001). Advanced oxidation protein products (AOPPs) increased only in the brain ( p < 0.001). DHA reduced these changes in MDA and AOPP values ( p < 0.05–0.001), while it increased CAT, SOD and GPx concentrations ( p < 0.05–0.001). Similarly, DHA prevented the decreases in body weight, body temperature and locomotor activities caused by CPF at 100 mg/kg and 400 mg/kg dosages ( p < 0.05–0.001). Similar to the physiological and biochemical changes, the histopathological damage scores, which increased with CPF ( p < 0.05–0.01), decreased at all three dosages of DHA ( p < 0.05–0.01). Our findings suggest that DHA, by supporting the antioxidant mechanism, reduces toxicity caused by CPF.

Protective Effects of Omega-3 Fatty Acid on Salt Induced Microscopic Changes in Femur of Female Sprague Dawley Rats

2021 ◽  
Vol 15 (12) ◽  
pp. 3522-3523
Author(s):  
Kaukab Anjum ◽  
Nomana Mahmood ◽  
Sumaira Abbasi ◽  
Ayesha Yasser ◽  
Iram Zakria ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cortical Bone ◽  
High Salt ◽  
Female Rats ◽  
Protective Effects ◽  
Omega 3 ◽  
Bone Thickness ◽  
Omega 3 Fatty Acid ◽  
Cortical Bone Thickness ◽  
Group B

Osteoporosis is a major risk factor for fracture affects an enormous number of people of both genders worldwide. Objectives: To evaluate the shielding effect of omega-3 fatty acids on high salt induced histological findings in femur of rats. Study Design: Randomized Control Trial. Methodology: Female rats (n=30) were divided into three groups. Group A recieved high salt diet (8%NaCl) while group B recieved omega-3-treated salt loaded diet receiving 260 mg/kg body weight with 8% NaCl solution (8 weeks), control group received standard diet. Tissue from mid shaft and proximal end of femur was obtained to study the osteoblast number, mid cortical bone thickness and trabecular bone architecture. Statistical analysis: SPSS software, v 21 analyzed data. Results: Protective effects were seen in Omega-3 fatty acid supplemented experimental group B with increase in osteoblast number, mid cortical bone thickness and increase in microstructure of trabeculae. Conclusion: We concluded that dietary nutrient like omega-3 fatty acid is a helpful tool in eliminating adverse effects of salt on bones by enhancing osteoblastic activity thus reducing its remodeling. Keywords: Bone, Osteoblast, Omega-3 fatty acid, Salt and Trabeculae.

scholarly journals Effect of omega-3 fatty acid supplementation on serum fasting glucose and HbA1c levels in type-2 Diabetes Mellitus

2019 ◽  
Vol 14 (1) ◽  
pp. 33-37
Author(s):  
Tamanna Binte Habib ◽  
Qazi Shamima Akhter
Keyword(s):  
Fatty Acid ◽  
Diabetic Patient ◽  
Control Group ◽  
Diabetic Patients ◽  
Omega 3 ◽  
Omega 3 Fatty Acid ◽  
Type 2 Dm ◽  
Type 2 Diabetic ◽  
Hba1c Levels

Background: Diabetes Mellitus (DM) is a major non-communicable health problem that have effects on glycemic status. Oral omega-3 fatty acid supplementation may improve glycemic status in diabetic patient. Objective: To observe the effect of supplementation of omega-3 fatty acid on fasting serum glucose (FSG) and glycosylated hemoglobin (HbA1c) levels in type 2 DM. Methods: This prospective interventional study was conducted from January 2017 to December 2017. A total numbers of 52 diagnosed type 2 diabetic patients(type-2 DM) of both sexes were selected with age 40 to 50 years. Among them, 27 type 2 diabetic patient with supplementation of omega 3 fatty acid (2g/day) for 12 weeks was considered as study group. Another 25 type 2 diabetic patients without supplementation of omega 3 fatty acid was considered as control group. The study subjects were selected from Outpatient Department of Endocrinology, Dhaka Medical College Hospital, Dhaka and personal contact from Dhaka city. FSG was estimated by enzymatic colorimetric method and HbA1c was estimated by turbidimetric inhibition immunoassay method. The study parameters were measured at base line and after 12 weeks of study period in both groups. For statistical analysis, Student’s paired ‘t’ test and Student’s unpaired ‘t’ test were performed as applicable using SPSS for windows version 16.0. Results: In this study serum FSG and HbA1c levels were significantly lower (p<.001) in diabetic patients after supplementation with omega-3 fatty acid in comparison to that of their baseline value. Again, after 12 weeks, FSG and HbA1c levels significantly decreased (p<.001) in omega-3 fatty acid supplemented diabetic patients compared to control group. In control group, there was no significant change in FSG and HbA1c levels between baseline and after 3 months of follow-up. Conclusion: From the results of the study, it can be concluded that omega-3 fatty acid may improve serum FSG and HbA1c levels in type 2 diabetic patient and may be helpful to minimize the complications of type-2 DM. J Bangladesh Soc Physiol. 2019, June; 14(1): 33-37

scholarly journals Effect of Omega-3 Fatty Acid on STAMP2 Expression in the Heart and Kidney of 5/6 Nephrectomy Rat Model

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 398 ◽  
Author(s):  
Hye Lee ◽  
Su Lee ◽  
Mi Lee ◽  
Young Son ◽  
Seong Kim ◽  
...  
Keyword(s):  
Metabolic Disease ◽  
Fatty Acid ◽  
Metabolic Disorders ◽  
Transmembrane Protein ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Sham Control ◽  
Omega 3 Fatty Acid ◽  
Lipid Disorders ◽  
Sprague Dawley Rats

Six transmembrane protein of prostate 2 (STAMP2) is a critical modulator of inflammation and metabolism in adipose tissue. There are no data on the expression of STAMP2 in chronic kidney disease, which is an inflammatory disease related to metabolic disorders. This study aimed to investigate STAMP2 expression in the kidney and heart in 5/6 nephrectomy (Nx) rats, and the effect of omega-3 fatty acid (FA) on STAMP2 expression. Male Sprague Dawley rats were divided into three groups: sham control (0.9% saline), 5/6 Nx (0.9% saline), and 5/6 Nx treated with omega-3 FA (300 mg per kg per day by gastric gavage). The expression of STAMP2 in the kidney and heart were examined by western blotting. Serum creatinine levels were higher in 5/6 Nx rats than in controls. Compared with sham controls, the expression of IκB, NF-κB, NOX4, SREBP-1, and LXR were upregulated and STAMP2 and phosphorylated-AMPK expression were downregulated in the kidney and heart of 5/6 Nx rats. Omega-3 FA supplementation prevented these changes in biomarkers related to inflammation and metabolic lipid disorders. Omega 3-FA supplementation induced the upregulation of STAMP2 protein in 5/6 Nx rats, which was associated with an attenuation of inflammation- and metabolic disease-related markers.

scholarly journals Parenteral Omega-3 Fatty Acid Supplementation Improves Outcome of Sepsis: A Real-World, Retrospective Cohort Study

2021 ◽  
Author(s):  
Youlian Chen ◽  
HuaiSheng Chen ◽  
YiChun Jiang ◽  
XueMing Tang ◽  
YongHui Ma ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Information System ◽  
Fatty Acid ◽  
Treatment Group ◽  
Mortality Rate ◽  
Control Group ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

Abstract Objectives: The role of omega-3 fatty acids in the treatment of sepsis is always on paradox. we tried to retrieve and download the patients’ data in a certain period through the hospital information system, used data sorting so as to screen out the patients with sepsis so as to find out the role of omega-3 fatty acids in sepsis. Methods: Through the hospital information system, retrieve and include the patients who were admitted to the Department of critical medicine of Shenzhen People's Hospital from December 2016 to June 2019, screen out patients diagnosed with sepsis according to a certain criterion. The patients were grouped by whether they were applicated with omega-3 fatty acid or not. Results: A total of 1733 cases included into analysis, among of whom 303 cases were applicated with omega-3 fatty acid. The amounts and baseline conditions between both groups were imbalance. Severity of omega-3 fatty acid group was higher than that of control group. Chi-square test found that the mortality rate of omega-3 fatty acid was higher than that of control group (p < 0.0001). But age, gender, whether there is abdominal infection, whether there is septicemia, shock, the need for mechanical ventilation, and the need for renal replacement therapy may all affect the prognosis of the patients. If these factors were used as covariates, multiple logistic regression analysis showed that there was no significant difference in mortality rate between the treatment group and the control group (P = 0.574). Survival analysis showed that the survival rate of treatment group was higher than that of the control group when at the end of total treatment duration (P = 0.035). Conclusion: For patients with more severe sepsis, doctors are more likely to use omega-3 fatty acids in the early stage. Omega-3 fatty acids may improve the long-term prognosis of sepsis, but the conclusion still needs to be accepted carefully.

scholarly journals Parenteral supplementation of omega-3 fatty acid on the prognosis of sepsis: a real world retrospective study

2020 ◽  
Author(s):  
Chen Youlian ◽  
Huaisheng Chen ◽  
XunFa Liu ◽  
YiChun Jiang ◽  
XueMing Tang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Information System ◽  
Fatty Acid ◽  
Treatment Group ◽  
Mortality Rate ◽  
Control Group ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

Abstract Objectives: The role of omega-3 fatty acids in the treatment of sepsis is always on paradox. we tried to retrieve and download the patients’ data in a certain period through the hospital information system, used data sorting so as to screen out the patients with sepsis so as to find out the role of omega-3 fatty acids in sepsis.Methods: Through the hospital information system, retrieve and include the patients who were admitted to the Department of critical medicine of Shenzhen People's Hospital from December 2016 to June 2019, screen out patients diagnosed with sepsis according to a certain criterion. The patients were grouped by whether they were applicated with omega-3 fatty acid or not.Results: A total of 1733 cases included into analysis, among of whom 303 cases were applicated with omega-3 fatty acid. The amounts and baseline conditions between both groups were imbalance. Severity of omega-3 fatty acid group was higher than that of control group. Chi-square test found that the mortality rate of omega-3 fatty acid was higher than that of control group (p < 0.0001). But age, gender, whether there is abdominal infection, whether there is septicemia, shock, the need for mechanical ventilation, and the need for renal replacement therapy may all affect the prognosis of the patients. If these factors were used as covariates, multiple logistic regression analysis showed that there was no significant difference in mortality rate between the treatment group and the control group (P = 0.574). Survival analysis showed that the survival rate of treatment group was higher than that of the control group when at the end of total treatment duration (P = 0.035).Conclusion: For patients with more severe sepsis, doctors are more likely to use omega-3 fatty acids in the early stage. Omega-3 fatty acids may improve the long-term prognosis of sepsis, but the conclusion still needs to be accepted carefully.

scholarly journals Omega-3 Fatty Acid Enriched Chevon (Goat Meat) Lowers Plasma Cholesterol Levels and Alters Gene Expressions in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mahdi Ebrahimi ◽  
Mohamed Ali Rajion ◽  
Goh Yong Meng ◽  
Abdoreza Soleimani Farjam
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Plasma Cholesterol ◽  
Control Group ◽  
Omega 3 ◽  
Goat Meat ◽  
Omega 3 Fatty Acid ◽  
Cholesterol Levels ◽  
Oil Palm Frond ◽  
Palm Frond

In this study, control chevon (goat meat) and omega-3 fatty acid enriched chevon were obtained from goats fed a 50% oil palm frond diet and commercial goat concentrate for 100 days, respectively. Goats fed the 50% oil palm frond diet contained high amounts ofα-linolenic acid (ALA) in their meat compared to goats fed the control diet. The chevon was then used to prepare two types of pellets (control or enriched chevon) that were then fed to twenty-male-four-month-oldSprague-Dawleyrats (n=10in each group) for 12 weeks to evaluate their effects on plasma cholesterol levels, tissue fatty acids, and gene expression. There was a significant increase in ALA and docosahexaenoic acid (DHA) in the muscle tissues and liver of the rats fed the enriched chevon compared with the control group. Plasma cholesterol also decreased (P<0.05) in rats fed the enriched chevon compared to the control group. The rat pellets containing enriched chevon significantly upregulated the key transcription factor PPAR-γand downregulated SREBP-1c expression relative to the control group. The results showed that the omega-3 fatty acid enriched chevon increased the omega-3 fatty acids in the rat tissues and altered PPAR-γand SREBP-1c genes expression.

scholarly journals Combined Treatment with Omega-3 Fatty Acid and Cholecalciferol Increases 1,25-Dihydroxyvitamin D Levels by Modulating Dysregulation of Vitamin D Metabolism in 5/6 Nephrectomy Rats

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2903
Author(s):  
Su Mi Lee ◽  
Mi Hwa Lee ◽  
Young Ki Son ◽  
Seong Eun Kim ◽  
Won Suk An
Keyword(s):  
Vitamin D ◽  
Fatty Acid ◽  
Combined Treatment ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Vitamin D Metabolism ◽  
Omega 3 Fatty Acid ◽  
Hydroxyvitamin D ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D

The protein 1α-hydroxylase (CYP27B1) was expressed in liver and omega-3 fatty acid (FA) elevated 1,25-dihydroxyvitamin D [1,25(OH)2D] levels in dialysis patients. The aim of this study was to determine whether omega-3 FA and cholecalciferol have effects on vitamin D metabolism related to CYP27B1 and 24-hydroxylase (CYP24) activities in the kidney and liver of 5/6 nephrectomy (Nx) rats. Male Sprague–Dawley rats were divided into the following groups: sham control, 5/6 Nx, 5/6 Nx treated with cholecalciferol, 5/6 Nx treated with omega-3 FA, and 5/6 Nx treated with cholecalciferol/omega-3 FA. CYP27B1 and CYP24 expression were measured in the liver and kidney. Further, 1,25(OH)2D and 25-hydroxyvitamin D [25(OH)D] levels were measured in serum. Among Nx groups, 1,25(OH)2D and 25(OH)D levels were lowest in the 5/6 Nx group. CYP24 expression was increased in the kidney of the 5/6 Nx rat model, which was found to be reversed by omega-3 FA or cholecalciferol/omega-3 FA supplementation. Decreased CYP27B1 expression was observed in the liver of the 5/6 Nx rats and its expression was recovered by supplementation with cholecalciferol/omega-3 FA. In conclusion, omega-3 FA and cholecalciferol may synergistically increase 1,25(OH)2D levels by inhibiting CYP24 expression in the kidney and liver and activating CYP27B1 expression in the liver of 5/6 Nx rats.

scholarly journals The effect of fish oil capsule on serum total cholesterol and triglycride levels in type-2 diabetic patient

2018 ◽  
Vol 13 (2) ◽  
pp. 73-77
Author(s):  
Tamanna Binte Habib ◽  
Qazi Shamima Akhter
Keyword(s):  
Fatty Acid ◽  
Diabetic Patient ◽  
Control Group ◽  
Diabetic Patients ◽  
Omega 3 ◽  
Type 2 Diabetic Patients ◽  
Omega 3 Fatty Acid ◽  
Type 2 Dm ◽  
Type 2 Diabetic

Background: Diabetes Mellitus (DM) is one of the common metabolic disorders that have effects on lipid metabolism. Fish oil supplementation may improve lipid abnormalities in diabetic patient.Objective: To observe the effect of supplementation of omega-3 fatty acid on total cholesterol (TC) and triglyceride(TG) level in type 2 DM. Methods: A prospective interventional study was conducted from January 2017 to December 2017. A total numbers of 52 diagnosed type 2 diabetic patients of both sexes were selected with age 40 to 50 years. Among them, 27 type 2 diabetic patient with supplementation of omega 3 fatty acid (2g/ day) for 12 weeks was considered as study group. Another 25 type 2 diabetic patients without supplementation of omega 3 fatty acid was considered as control group. The study subjects were selected from Outpatient Department of Endocrinology, Dhaka Medical College Hospital, Dhaka and personal contact from Dhaka city.Serum TC and TG were estimated by enzymatic endpoint method in autoanalyzer. The study parameters were measured at base line and after 12 weeks of study period in both groups. For statistical analysis, Paired Student’s‘t’ test and Unpaired Student’s ‘t’ test were performed as applicable using SPSS for windows version 16.0. Results: In this study serumTC and TG levels were significantly lower (p<.001) in diabetic patients after supplementation with omega- 3 fatty acid in comparison to that of their baseline value.Again, after 12 weeks, serum TC and TG levels were significantly lower (p<.001) in diabetic patients after supplementation with omega-3 fatty acid in comparison to control group. In control group, serum TC and TG levels were almost similar both at baseline and after 3 months of follow-up. Conclusion: After analyzing the results of the study, it can be concluded that omega-3 fatty acid may improve serum TC and TG levels in type 2 diabetic patient and may be helpful to minimize the complications of type-2 DM. J Bangladesh Soc Physiol. 2018, December; 13(2): 73-77

Sign in / Sign up

Export Citation Format

Share Document