scholarly journals Importance of the free amine groups in acellular scaffold during tissue repairing or regeneration process

2019 ◽  
Vol 34 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Tengfei Pan ◽  
Jian Tao ◽  
Qingqiang Meng ◽  
Wei Zhao ◽  
Bingkui Song ◽  
...  
Keyword(s):  
Regeneration Process ◽  
Free Amine ◽  
Amine Groups ◽  
Acellular Scaffold

Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes

2020 ◽  
Vol 61 (10) ◽  
pp. 1775-1787
Author(s):  
Angus E Rae ◽  
Xiaoyang Wei ◽  
Neftali Flores-Rodriguez ◽  
David W McCurdy ◽  
David A Collings
Keyword(s):  
Plant Cell ◽  
Propidium Iodide ◽  
Cell Walls ◽  
Super Resolution ◽  
Plant Cell Walls ◽  
Free Amine ◽  
Resolution Imaging ◽  
Amine Groups ◽  
Schiff Reaction ◽  
Cell Wall Ingrowths

Abstract To understand plant growth and development, it is often necessary to investigate the organization of plant cells and plant cell walls. Plant cell walls are often fluorescently labeled for confocal imaging with the dye propidium iodide using a pseudo-Schiff reaction. This reaction binds free amine groups on dye molecules to aldehyde groups on cellulose that result from oxidation with periodic acid. We tested a range of fluorescent dyes carrying free amine groups for their ability to act as pseudo-Schiff reagents. Using the low-pH solution historically used for the Schiff reaction, these alternative dyes failed to label cell walls of Arabidopsis cotyledon vascular tissue as strongly as propidium iodide but replacing the acidic solution with water greatly improved fluorescence labeling. Under these conditions, rhodamine-123 provided improved staining of plant cell walls compared to propidium iodide. We also developed protocols for pseudo-Schiff labeling with ATTO 647N-amine, a dye compatible for super-resolution Stimulated Emission Depletion (STED) imaging. ATTO 647N-amine was used for super-resolution imaging of cell wall ingrowths that occur in phloem parenchyma transfer cells of Arabidopsis, structures whose small size is only slightly larger than the resolution limit of conventional confocal microscopy. Application of surface-rendering software demonstrated the increase in plasma membrane surface area as a consequence of wall ingrowth deposition and suggests that STED-based approaches will be useful for more detailed morphological analysis of wall ingrowth formation. These improvements in pseudo-Schiff labeling for conventional confocal microscopy and STED imaging will be broadly applicable for high-resolution imaging of plant cell walls.

scholarly journals Synthesis of diamine functionalised graphene oxide and its application in the fabrication of electrically conducting reduced graphene oxide/polymer nanocomposite films

2020 ◽  
Vol 2 (10) ◽  
pp. 4702-4712
Author(s):  
Namrata Maslekar ◽  
Rabiatul A. Mat Noor ◽  
Rhiannon P. Kuchel ◽  
Yin Yao ◽  
Per B. Zetterlund ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrical Properties ◽  
Reduced Graphene Oxide ◽  
Polymer Nanocomposite ◽  
Nanocomposite Films ◽  
Electrically Conducting ◽  
Free Amine ◽  
Reduced Graphene ◽  
Amine Groups

The study focussed on synthesis of colloidally stable diamine functionalised graphene oxide (GO) with dangling free amine groups, and exhibited physicochemical and electrical properties of these functionalised sheets in a polymer-based nanocomposite.

METABOLIC CHANGES ASSOCIATED WITH OZONE INJURY OF BEAN LEAVES

1972 ◽  
Vol 52 (4) ◽  
pp. 589-597 ◽  
Author(s):  
L. E. CRAKER ◽  
J. S. STARBUCK
Keyword(s):  
Air Pollution ◽  
Phaseolus Vulgaris ◽  
Oxidant Injury ◽  
Metabolic Processes ◽  
Ozone Injury ◽  
Free Amine ◽  
Bean Plants ◽  
Amine Groups ◽  
Ozone Stress ◽  
Bean Leaves

Metabolic processes in primary leaves of bean plants (Phaseolus vulgaris) were altered by ozone stress. Decreases in levels of ribonucleic acid (RNA) and protein, and increases in ribonuclease (RNase) and free amine groups were associated with visible oxidant injury to the leaves. It appears that some air pollution injury to plants may result from changes in metabolic processes.

scholarly journals Characterization of the oxidation products of the reaction between reduced glutathione and hypochlorous acid

1997 ◽  
Vol 326 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Christine C. WINTERBOURN ◽  
Stephen O. BRENNAN
Keyword(s):  
Molecular Mass ◽  
Hypochlorous Acid ◽  
Reduced Glutathione ◽  
Oxidation Products ◽  
Thiol Groups ◽  
Hydrogen Atoms ◽  
Free Amine ◽  
Amine Groups ◽  
Biological Substrates

Reduced glutathione (GSH) is one of the most preferred biological substrates of myeloperoxidase-derived hypochlorous acid and is a likely target for neutrophil oxidants. We have used HPLC to show that the oxidation of GSH by hypochlorous acid gives two major, stable products in addition to glutathione disulphide (GSSG). The most prevalent product lacks free amine and thiol groups, and was shown by electrospray MS to have a molecular mass of 337 Da. This corresponds to GSH with a gain of two oxygen atoms and a loss of two hydrogen atoms, and is consistent with the product being an internal sulphonamide. The other novel product has a molecular mass of 644 Da, and has amine groups but no free thiols. These properties are consistent with it being glutathione thiolsulphonate. Whereas GSSG in the cell is recycled enzymically, formation of these higher oxidation products is likely to be irreversible. Hypochlorous acid, therefore, could compromise the cell by depleting GSH. The putative sulphonamide may be unique for oxidation by hypochlorous acid and thus provide a useful marker of neutrophil oxidant activity.

scholarly journals Functionalization and Modification of Bitumen by Silica Nanoparticles

2020 ◽  
Vol 10 (17) ◽  
pp. 6065
Author(s):  
Ainur Zhambolova ◽  
Anna Lisa Vocaturo ◽  
Yerbol Tileuberdi ◽  
Yerdos Ongarbayev ◽  
Paolino Caputo ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Rheological Properties ◽  
Crucial Role ◽  
Slight Improvement ◽  
Experimental Conditions ◽  
Alkyl Chains ◽  
Free Amine ◽  
Amine Groups ◽  
Significant Change

A study on the effect of silica nanoparticles (SNPs) dispersion in bitumen is herein reported. First, the size of the nanoparticles was finely tuned by controlling the experimental conditions during their synthesis, obtaining spherical SNPs with diameter ranging from 95 up to 900 nm. Subsequently, SNPs were embedded with peripheral amine groups by using APTES (3-aminopropyltriethoxysilane) as functionalized agent (NH2@SNP), and ultimately long alkyl chains were grafted by reacting the free amine with an alkylated aldehyde (C14N@SNP). All SNPs (ca. 1 wt%.) were dispersed in bitumen to probe their effect on the rheological properties of bitumen. No significant change in the thermorheological properties of bitumen was observed upon varying the size of the SNPs. Slight improvement was observed when using NH2@SNPs, while the best results were obtained by using C14N@SNPs, showing the crucial role that hydrophobic substituents play in bitumen binders which leads to significant improvements.

scholarly journals New Chloramphenicol Derivatives with a Modified Dichloroacetyl Tail as Potential Antimicrobial Agents

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 394
Author(s):  
Artemis Tsirogianni ◽  
Georgia G. Kournoutou ◽  
Anthony Bougas ◽  
Eleni Poulou-Sidiropoulou ◽  
George Dinos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Amine Group ◽  
Antibiotic Development ◽  
Beta Alanine ◽  
Free Amine ◽  
Free Amine Group ◽  
Amine Groups

To combat the dangerously increasing pathogenic resistance to antibiotics, we developed new pharmacophores by chemically modifying a known antibiotic, which remains to this day the most familiar and productive way for novel antibiotic development. We used as a starting material the chloramphenicol base, which is the free amine group counterpart of the known chloramphenicol molecule antibiotic upon removal of its dichloroacetyl tail. To this free amine group, we tethered alpha- and beta-amino acids, mainly glycine, lysine, histidine, ornithine and/or beta-alanine. Furthermore, we introduced additional modifications to the newly incorporated amine groups either with protecting groups triphenylmethyl- (Trt) and tert-butoxycarbonyl- (Boc) or with the dichloroacetic group found also in the chloramphenicol molecule. The antimicrobial activity of all compounds was tested both in vivo and in vitro, and according to the results, the bis-dichloroacetyl derivative of ornithine displayed the highest antimicrobial activity both in vivo and in vitro and seems to be a dynamic new pharmacophore with room for further modification and development.

scholarly journals Effects of Dexamethasone on Regeneration Process after Sciatic Nerve Transect and Acellular Scaffold Graft in a Rat Animal Model

2018 ◽  
Vol 26 (4) ◽  
pp. 104-115
Author(s):  
mohammad bagher Ghayour ◽  
Arash Abdolmaleki ◽  
Morteza Behnamrasouli ◽  
Ali Moghimi ◽  
Naser Mahdavishahri ◽  
...  
Keyword(s):  
Animal Model ◽  
Sciatic Nerve ◽  
Regeneration Process ◽  
Acellular Scaffold ◽  
Rat Animal Model

scholarly journals A practical solution for preserving single cells for RNA sequencing

2017 ◽  
Author(s):  
Moustafa Attar ◽  
Eshita Sharma ◽  
Shuqiang Li ◽  
Claire Bryer ◽  
Laura Cubitt ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Rna Seq ◽  
Rna Integrity ◽  
Tissue Integrity ◽  
Free Amine ◽  
Gene Level ◽  
Single Cell Isolation ◽  
Amine Groups ◽  
Cell Preservation

AbstractThe design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant’s Reagent, to stabilise cell samples for single-cell transcriptomic applications. DSP is a reversible cross-linker of free amine groups that has previously been shown to preserve tissue integrity for histology while maintaining RNA integrity and yield in bulk RNA extractions. Although RNA-seq data from DSP-fixed single cells appears to be prone to characteristic artefacts, such as slightly reduced yield of cDNA and a detectable 3’ bias in comparison with fresh cells, cell preservation using DSP does not appear to substantially reduce RNA complexity at the gene level. In addition, there is evidence that instantaneous fixation of cells can reduce inter-cell technical variability. The ability of DSP-fixed cells to retain commonly used dyes, such as propidium iodide, enables the tracking of experimental sub-populations and the recording of cell viability at the point of fixation. Preserving cells using DSP will remove several barriers in the staging of single-cell experiments, including the transport of samples and the scheduling of shared equipment for downstream single-cell isolation and processing.

scholarly journals Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 470 ◽  
Author(s):  
Bolat Sultankulov ◽  
Dmitriy Berillo ◽  
Karina Sultankulova ◽  
Tursonjan Tokay ◽  
Arman Saparov
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Tissue Repair ◽  
Chemically Modified ◽  
Tissue Repair And Regeneration ◽  
Current Review ◽  
Free Amine ◽  
Natural Polysaccharide ◽  
Amine Groups ◽  
Made In

Over the last few decades, chitosan has become a good candidate for tissue engineering applications. Derived from chitin, chitosan is a unique natural polysaccharide with outstanding properties in line with excellent biodegradability, biocompatibility, and antimicrobial activity. Due to the presence of free amine groups in its backbone chain, chitosan could be further chemically modified to possess additional functional properties useful for the development of different biomaterials in regenerative medicine. In the current review, we will highlight the progress made in the development of chitosan-containing bioscaffolds, such as gels, sponges, films, and fibers, and their possible applications in tissue repair and regeneration, as well as the use of chitosan as a component for drug delivery applications.

Sign in / Sign up

Export Citation Format

Share Document