Role of Suspected Periodontopathogens in Microbiological Monitoring of Periodontitis

1993 ◽  
Vol 7 (2) ◽  
pp. 163-174 ◽  
Author(s):  
G. Dahlén
Keyword(s):  
Periodontal Disease ◽  
Treatment Decision ◽  
Fusobacterium Nucleatum ◽  
The Body ◽  
Actinobacillus Actinomycetemcomitans ◽  
Risk Markers ◽  
Campylobacter Rectus ◽  
Localized Juvenile Periodontitis ◽  
Host Parasite

Periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. Although a specificity to some degree is found for Actinobacillus actinomycetemcomitans in localized juvenile periodontitis (LJP), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. What we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshold of tissue integrity. This hypothesis is concomitant with the view of periodontal disease as a polymicrobial infection, predominantly anaerobic, which occurs commonly in the oral cavity or elsewhere in the body. Some micro-organisms (risk markers) occur more frequently than others and may significantly determine the outcome of this host-parasite interaction. Microbiological sampling and analysis seem to be of limited value in risk assessment; however, they can be used as tools in diagnosis in LJP patients and acute infections, and in treatment decision and therapy control in "refractory" patients. Suspected pathogens (risk markers) are Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and some species of spirochetes, while the roles of Prevotella intermedia, Bacteroides forsythus, Fusobacterium nucleatum, Campylobacter rectus, and Peptostreptococcus micros are more uncertain. The presence of periodontopathogens as well as enterics, Staphylococcus aureus and Candida especially, should be considered in patients with systemic individual disorders-e.g., diabetes mellitus, neutropenia, agranulocytosis, and AIDS-or with implants.

scholarly journals MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Jageer Chinna ◽  
Jannat Sharma
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Disease Process ◽  
Fusobacterium Nucleatum ◽  
Host Cells ◽  
Bacterial Invasion ◽  
Periodontal Pathogens ◽  
Tissue Destruction ◽  
Campylobacter Rectus ◽  
Periodontal Tissues

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

scholarly journals DETECTION OF BACTERIAL SPECIES ASSOCIATED WITH PERIODONTAL DISEASE IN A GROUP OF PATIENTS

2021 ◽  
Vol 67 (2) ◽  
pp. 128-134
Author(s):  
Cristina Vodiță ◽  
◽  
Doina Lucia Ghergic ◽  
Emanuel Alin Vodiță ◽  
Raluca Monica Comăneanu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Treatment Session ◽  
Prevotella Intermedia ◽  
Bacterial Strains ◽  
Tannerella Forsythia ◽  
Campylobacter Rectus ◽  
Number Of Patients

Objectives. The study aimed to detect the presence/quantification of the 12 bacterial strains more frequently involved in the occurrence and aggravation of periodontal disease in a group of patients. Material and method. The study included 55 patients of both sexes, from the portfolio of the „Dr. Vodiță“ Dental Clinic, with chronic marginal periodontitis, who had not been under regular dental control and hygiene for at least 2 years. During the first treatment session, fluid was collected from each patient from the periodontal bags using sterile paper cones from the collection putty. Subsequently, fluid-soaked paper cones from periodontal bags were sent to the Genetic Lab in Bucharest for DNA extraction and quantification of 12 bacterial species, more common in the etiology of periodontal disease. For the correctness of the results obtained, it is necessary that patients have not taken antibiotics in the last 3 weeks. The data were analyzed and statistically processed with the Microsoft Excel 2016 program. Results. The least common bacteria found were from the species: Capnocytophaga ochracea, Aggregatibacter actinomycetemcomitans, Eikanella corrodens and Campylobacter rectus, closely followed by Capnocytophaga gingivalis, Prevotella intermedia and Capnocytophaga sputigena. The rest of the bacterial species were detected much more frequently. Discussions. In the analyzed group there was no monoinfection with Aggregatibacter actinomycetemcomitans. Porphyromonas gingivalis had an important presence in the studied group, registering increased levels in 89% of cases. In our group, Treponema denticola was present in increased amounts in 76.36% of cases. Tannerella forsythia was present in 92.72% of cases in elevated concentrations. Eikanella corrodens and Campylobacter rectus were present in 18.18% of the cases studied in high quantities. Prevotella intermedia was present in significant amounts in 34.54% of cases. Fusobacterium nucleatum was present in high concentrations in 98.18% of the cases studied. Prevotella nigrescens was present in increased amounts in 56.36% of cases. Capnocytophaga ochracea was present in 9.09% of cases in high amounts, Capnocytophaga sputigena in 38.18% of cases, and Capnocytophaga gingivalis was detected in 24.45% of cases. Conclusions. From the class of bacteria with high pathogenicity, we most frequently encountered in the studied group Tannerella forsythia. From the class of bacteria with moderate pathogenicity, we encountered the most common Fusobacterium nucleatum. From the class of bacteria with low pathogenicity, we encountered the most common Capnocytophaga sputigena. In order to validate the results obtained, it is necessary to extend the study to a larger number of patients.

scholarly journals Microbiological study of periodontal disease in populations with HIV: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Nasrin Keshavarz Valian ◽  
Behzad houshmand ◽  
Mohammadreza Talebi Ardakani ◽  
Shima Mahmoudi
Keyword(s):  
Systematic Review ◽  
Periodontal Disease ◽  
Meta Analysis ◽  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Inclusion Criteria ◽  
Campylobacter Rectus ◽  
Pooled Prevalence ◽  
High Prevalence ◽  
Male Patients

Abstract Background: No systematic review/meta-analysis has been conducted on the microbiological profile associated with the occurrence of periodontitis in patients with HIV. The aim of this study was to evaluate the prevalence of identified bacteria in HIV-infected patients with periodontal disease.Methods: Three English electronic databases (MEDLINE (via PubMed), SCOPUS, and Web of Science) were searched systematically from the beginning to 13 February 2021. The frequency of each identified bacteria in HIV-infected patients with periodontal disease was extracted. All meta-analysis methods were performed using STATA software. Results: Twenty-two articles met inclusion criteria and enrolled into the systematic review. This review analyzed a total of 965 HIV-infected patients with periodontitis. The prevalence of periodontitis was higher in HIV-infected male patients (83% (CI95%: 76-88%)) compared to females (28% (CI95%: 17-39%)). In our study, the pooled prevalence of necrotizing ulcerative periodontitis and necrotizing ulcerative gingivitis in patients with HIV infection was 67% (CI95%: 52-82%) and 60% (CI95%: 45-74%), while a lower prevalence of linear gingivitis erythema was reported (11% (CI95%: 5-18%)). More than 140 bacterial species were identified from HIV-infected patients with periodontal disease. High prevalence of Tannerella forsythia (51% (CI95%: 5-96%)), Fusobacterium nucleatum (50% (CI95%: 21-78%)), Prevotella intermedia (50% (CI95%: 32-68%)), Peptostreptococcus micros (44% (CI95%: 25-65%)), Campylobacter rectus (35% (CI95%: 25-45%)), and Fusobacterium spp. (35% (CI95%: 3-78%)) in HIV-infected patients with periodontal disease was found.Conclusion: Our study demonstrated that the prevalence of red and orange complex of bacteria in HIV patients with periodontal disease is relatively high.

scholarly journals Induction of Apoptotic Cell Death in Peripheral Blood Mononuclear and Polymorphonuclear Cells by an Oral Bacterium,Fusobacterium nucleatum

2000 ◽  
Vol 68 (4) ◽  
pp. 1893-1898 ◽  
Author(s):  
Anahid Jewett ◽  
Wyatt R. Hume ◽  
Ho Le ◽  
Tri N. Huynh ◽  
Yiping W. Han ◽  
...  
Keyword(s):  
Cell Death ◽  
Periodontal Disease ◽  
Peripheral Blood ◽  
Cell Apoptosis ◽  
Apoptotic Cell ◽  
Fusobacterium Nucleatum ◽  
Polymorphonuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Oral Bacterium

ABSTRACT It is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of competent immune effector cells. In this paper we present evidence for the immunosuppressive role of Fusobacterium nucleatum, a gram-negative oral bacterium which plays an important role in the generation of periodontal disease. Our studies indicate that the immunosuppressive role of F. nucleatum is largely due to the ability of this organism to induce apoptotic cell death in peripheral blood mononuclear cells (PBMCs) and in polymorphonuclear cells (PMNs). F. nucleatum treatment induced apoptosis of PBMCs and PMNs as assessed by an increase in subdiploid DNA content determined by DNA fragmentation and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling assays. The ability of F. nucleatum to induce apoptosis was abolished by either heat treatment or proteinase digestion but was retained after formaldehyde treatment, suggesting that a heat-labile surface protein component is responsible for bacterium-mediated cell apoptosis. The data also indicated that F. nucleatum-induced cell apoptosis requires activation of caspases and is protected by NF-κB. Possible mechanisms of F. nucleatum's role in the pathogenesis of periodontal disease are discussed.

scholarly journals Virulence factors of Actinobacillus actinomycetemcomitans: other putative factors

2000 ◽  
Vol 14 (1) ◽  
pp. 05-11
Author(s):  
Mario Julio AVILA-CAMPOS ◽  
Maria Regina Lorenzetti SIMIONATO ◽  
Silvana CAI ◽  
Márcia Pinto Alves MAYER ◽  
José Luiz DE LORENZO ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Periodontal Disease ◽  
Virulence Factors ◽  
Causative Agent ◽  
Tissue Repair ◽  
Bacteriocin Production ◽  
Actinobacillus Actinomycetemcomitans ◽  
Blood Types ◽  
Wide Range ◽  
Localized Juvenile Periodontitis

Actinobacillus actinomycetemcomitans is implicated as the causative agent of localized juvenile periodontitis. This organism possesses a large number of virulence factors with a wide range of activities and also interfere with tissue repair. Fifty isolates of A. actinomycetemcomitans from 20 periodontal patients were examined to evaluate other putative virulence factors. In this study, the capsule, DNase, coagulase, fibrinolysin, proteolytic, haemolysin and bacteriocin production, haemagglutination, serum sensitivity, epithelial cells attachment, hydrophobicity and virulence of the A. actinomycetemcomitans isolates were evaluated. All the isolates were resistant to the different tested sera. 70% to 94% were alpha-haemolytics and agglutinated all blood types. Most of isolates produced antagonistic substances and they had a low hydrophobicity. None of the isolates was pathogenic for mice. Little is known as to wether these factors may act in the development of periodontal disease, and further studies are required for an application in pathogenic and systematic terms.

The role of Actinobacillus actinomycetemcomitans in periodontal disease

Infection ◽  
1989 ◽  
Vol 17 (4) ◽  
pp. 269-271 ◽  
Author(s):  
J. de Graaff ◽  
A. J. Winkelhoff ◽  
R. J. Goene
Keyword(s):  
Periodontal Disease ◽  
Actinobacillus Actinomycetemcomitans

Role of regulatory peptides in parasitic platyhelminths and their vertebrate hosts: possible novel factors in host-parasite interactions

Parasitology ◽  
1991 ◽  
Vol 102 (S1) ◽  
pp. S93-S105 ◽  
Author(s):  
C. Shaw ◽  
C. F. Johnston
Keyword(s):  
Regulatory Peptides ◽  
The Body ◽  
Neural Pathways ◽  
Target Tissue ◽  
Original Stimulus ◽  
Classical Work ◽  
Distant Target ◽  
Vertebrate Hosts ◽  
Host Parasite

The study of regulatory peptides has its origins in the classical work of Bayliss & Starling (1902). Their pioneering work on the presence of a factor in intestinal extracts which, when injected into the bloodstream of experimental animals, elicited pancreatic secretion, led to the genesis of the concept of the hormone, i.e. a chemical messenger which is released from one part of the body in response to a stimulus to travel in the bloodstream to a distant target tissue where it would elicit a physiological response appropriate to the original stimulus. In keeping with accepted scientific tradition, this concept had its critics. Pavlov, who had been studying secretory stimulation from a different perspective, concluded from his work on salivation in dogs, that this was mediated via neural pathways. With hindsight, and the benefits of knowledge obtained from nearly a century of scientific research, we now know that these pioneers were in actual fact studying different aspects of the same process and that both theories were complementary. In fact, it is becoming increasingly difficult to ascribe secretory control to either circulating or neuronal factors as both appear to be intimately involved in regulation.

scholarly journals The Role of the Leucocytosis Parameter in the Occurrence of Pregnancy Complications in Women with Periodontal Disease

2019 ◽  
Vol 70 (11) ◽  
pp. 3967-3970
Author(s):  
Camelia Fiera Maglaviceanu ◽  
Edwin Sever Bechir ◽  
Mihaela Jana Tuculina ◽  
Constantin Daguci ◽  
Ionela Teodora Dascalu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Pregnancy Complications ◽  
Intrauterine Growth Restriction ◽  
The Body ◽  
Intrauterine Growth ◽  
Obstetrics And Gynaecology ◽  
Severe Periodontitis ◽  
Significant Difference ◽  
Control Segment

For the present study, a total number of 109 pregnant patients in the second trimester of pregnancy were selected from the case studies of the Clinic of Obstetrics and Gynaecology of the Municipal Clinical Hospital Filantropia in Craiova. Among these, 56 patients had gingivitis, 26 had mild, moderate, or severe periodontitis, and 27 patients were used as a control segment. Each patient had her leukocytes values tested and they had their oral cavity examined. Leukocyte neutrophils are the primary cells of the body�s defence system as well as primary protective cells against periodontal disease. Based on the study, we can say that there is a significant difference between the average number of leukocytes found in the four groups, because the result of the ANOVA test was below the threshold p [0.001. By analyzing the involvement of the leucocytosis parameter in the occurrence or presence of pregnancy complications, we found it present in periodontal disease pregnant women who had complications, namely: premature birth, preeclampsia, or intrauterine growth restriction.

scholarly journals Periodontal disease in relation to some systemic diseases

2003 ◽  
Vol 50 (4) ◽  
pp. 197-201
Author(s):  
Olivera Cerovic ◽  
Besir Ljuskovic ◽  
Vladan Kovacevic
Keyword(s):  
Risk Factor ◽  
Periodontal Disease ◽  
The Body ◽  
The Other ◽  
Systemic Diseases ◽  
General State ◽  
Blood Disorders ◽  
Periodontal Health ◽  
Periodontal Infection

Periodontal disease is closely related to the general state of the body because it significantly influences periodontal health, as periodontal health can have an influence on the state of the body. It is known that some systemic diseases can represent a risk factor for periodontal disease such as diabetes, blood disorders and immunodefficient disorders. Reducing defensive efficiency of the body, these diseases enable the onset and the development of periodontal disease. On the other hand, concerning the role of microorganisms in the onset and the development of periodontal disease there is justified suspicion that periodontal infection might endanger general state of the body and cause some systemic diseases, such as cardiovascular, respiratory, eye, renal, skin disease and the diseases of the musculoskeletal system. The awareness of connection between periodontal disease and some systemic diseases is of great significance for the diagnosis and the therapy of periodntal disease and the disorders it may cause.

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