Adenosine deaminase activity in pig saliva: analytical validation of two spectrophotometric assays

We validated 2 assays for the measurement of adenosine deaminase (ADA) activity in the saliva of pigs: the Giusti–Galanti manual method (ADA-GG) and a commercial automated assay (Diazyme Laboratories; ADA-D). Intra-assay coefficients of variation (CVs) were <7 and 9%, and interassay CVs were <12 and 5%, for ADA-GG and ADA-D, respectively. Accuracy was measured by 2 methods: recovery and linearity-under-dilution. Recovery was 82.4–106.8% for ADA-GG, and 92.8–107.9% for ADA-D. Serial dilutions showed R2 > 0.95 and 0.99 for ADA-GG and ADA-D, respectively. Linear regression between the methods gave R2 = 0.997 ( p < 0.0001), and a Bland–Altman plot showed a proportional bias of 112 IU/L (95% confidence interval of −99 to 322 IU/L) for ADA-D. No significant differences were observed between the results obtained by either method in saliva or serum. ADA activity was much higher in porcine saliva than in serum. Salivary ADA activity was significantly higher in lame pigs compared to healthy animals. However, serum ADA activity was significantly lower in lame pigs.

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Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2021-0324 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Lorena Franco-Martínez ◽

Fernando Tecles ◽

Alberto Torres-Cantero ◽

Enrique Bernal ◽

Indra San Lázaro ◽

...

Keyword(s):

Adenosine Deaminase ◽

Analytical Validation ◽

Mean Values ◽

Chemical Inactivation ◽

Non Invasive ◽

Automated Assay ◽

Coefficients Of Variation ◽

Recovery Percentage ◽

Triton X ◽

Potential Use

Abstract Objectives The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2. Results The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35). Conclusions tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease.

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Investigating the Dutch Movement-Specific Reinvestment Scale in people with stroke

Clinical Rehabilitation ◽

10.1177/0269215512448381 ◽

2012 ◽

Vol 27 (2) ◽

pp. 160-165 ◽

Cited By ~ 19

Author(s):

Melanie Kleynen ◽

Susy M Braun ◽

Anna JHM Beurskens ◽

Jeanine A Verbunt ◽

Rob A de Bie ◽

...

Keyword(s):

Confidence Interval ◽

Intraclass Correlation ◽

Correlation Coefficients ◽

Limits Of Agreement ◽

Rehabilitation Centre ◽

Altman Plot ◽

Rehabilitation Process ◽

Bland Altman Plot ◽

Intraclass Correlation Coefficients ◽

Conscious Control

Objective: Reinvestment is a phenomenon in which conscious control of movements that are best controlled automatically disrupts performance. The propensity for reinvestment may therefore play an important role in the movement rehabilitation process. The Movement-Specific Reinvestment Scale measures an individual’s propensity for reinvestment. The aim of this study was to translate the scale for use with Dutch participants with stroke and to assess its reliability. Design: A test–retest design. Setting: In community after discharge from rehabilitation centre. Subjects: Forty-five people with stroke. Measures: Reliability of the translated scale was assessed using intraclass correlation coefficients (ICC) and Bland–Altman plot. Results: The ICC was 0.85 (95% confidence interval (CI) 0.74–0.91). Limits of agreement ranged from −2.38 to 3.10. Conclusion: The Dutch Movement-Specific Reinvestment Scale appears to be a reliable tool with which to assess the propensity for movement-specific reinvestment by people with stroke.

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Using linear regression to assess dose-dependent bias on a Bland-Altman plot

Journal of Emergency and Critical Care Medicine ◽

10.21037/jeccm.2018.08.02 ◽

2018 ◽

Vol 2 ◽

pp. 68-68 ◽

Cited By ~ 6

Author(s):

Kwok M. Ho

Keyword(s):

Linear Regression ◽

Altman Plot ◽

Bland Altman Plot ◽

Dose Dependent

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Analytical validation of an automated assay for ferric-reducing ability of plasma in dog serum

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638717693883 ◽

2017 ◽

Vol 29 (4) ◽

pp. 574-578 ◽

Cited By ~ 7

Author(s):

Camila Peres Rubio ◽

Silvia Martinez-Subiela ◽

Josefa Hernández-Ruiz ◽

Asta Tvarijonaviciute ◽

José Joaquín Ceron

Keyword(s):

Detection Limit ◽

Analytical Validation ◽

Frap Assay ◽

Serial Sample ◽

Automated Assay ◽

Reducing Ability ◽

Coefficients Of Variation ◽

Healthy Dogs ◽

Interassay Precision

We performed analytical validation of an automated ferric-reducing ability of plasma (FRAP) assay in the serum of dogs. Intra- and interassay precision, accuracy, detection limit, and effects of hemolysis and lipemia were evaluated. Intra- and interassay coefficients of variation were <1% and <13%, respectively. The assay showed a high correlation with a FRAP assay described previously, and results were linear when serial sample dilutions were tested. The detection limit was lower than the values observed in sera from healthy dogs; decreased serum FRAP was found in dogs with leishmaniosis. Lipemia and hemolysis caused a significant increase in the results of the assay.

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Discovery of an adenosine deaminase activity in the preparates of human IgG3

Immunology Letters ◽

10.1016/s0165-2478(97)88469-6 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 403

Author(s):

A Zavialov

Keyword(s):

Adenosine Deaminase ◽

Adenosine Deaminase Activity

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Adenosine deaminase activity in rheumatoid pleural effusion.

Annals of the Rheumatic Diseases ◽

10.1136/ard.47.5.394 ◽

1988 ◽

Vol 47 (5) ◽

pp. 394-397 ◽

Cited By ~ 32

Author(s):

I Ocana ◽

E Ribera ◽

J M Martinez-Vazquez ◽

I Ruiz ◽

E Bejarano ◽

...

Keyword(s):

Pleural Effusion ◽

Adenosine Deaminase ◽

Adenosine Deaminase Activity

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Ethnic Specific body fat percent prediction equation as surrogate marker of obesity in Ethiopian adults

Journal of Health Population and Nutrition ◽

10.1186/s41043-021-00224-3 ◽

2021 ◽

Vol 40 (1) ◽

Author(s):

Makeda Sinaga ◽

Melese Sinaga Teshome ◽

Tilhun Yemane ◽

Elsah Tegene ◽

David Lindtsrom ◽

...

Keyword(s):

Body Fat ◽

Age Groups ◽

Surrogate Marker ◽

Cost Effective ◽

Prediction Equation ◽

Prediction Equations ◽

Altman Plot ◽

Cross Sectional ◽

Bland Altman Plot ◽

Body Fat Percent

Abstract Background Application of advanced body composition measurement methods is not practical in developing countries context due to cost and unavailability of facilities. This study generated ethnic specific body fat percent prediction equation for Ethiopian adults using appropriate data. Methods A cross-sectional study was carried ifrom February to April 2015 among 704 randomly selected adult employees of Jimma University. Ethnic specific Ethiopian body fat percent (BF%) prediction equation was developed using a multivariable linear regression model with measured BF% as dependent variable and age, sex, and body mass index as predictor variables. Agreement between fat percent measured using air displacement plethysmography and body fat percent estimated using Caucasian prediction equations was determined using Bland Altman plot. Results Comparison of ADP measured and predicted BF% showed that Caucasian prediction equation underestimated body fat percent among Ethiopian adults by 6.78% (P < 0.0001). This finding is consistent across all age groups and ethnicities in both sexes. Bland Altman plot did not show agreement between ADP and Caucasian prediction equation (mean difference = 6.7825) and some of the points are outside 95% confidence interval. The caucasian prediction equation significantly underestimates body fat percent in Ethiopian adults, which is consistent across all ethnic groups in the sample. The study developed Ethnic specific BF% prediction equations for Ethiopian adults. Conclusion The Caucasian prediction equation significantly underestimates body fat percent among Ethiopian adults regardless of ethnicity. Ethiopian ethnic-specific prediction equation can be used as a very simple, cheap, and cost-effective alternative for estimating body fat percent among Ethiopian adults for health care provision in the prevention of obesity and related morbidities and for research purposes.

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Assessing Overall Diet Quality: Development and Evaluation of the Performance of a Short Self-Administrated Questionnaire SCASA

Nutrients ◽

10.3390/nu13020677 ◽

2021 ◽

Vol 13 (2) ◽

pp. 677

Author(s):

Maaike Kruseman ◽

Angeline Chatelan ◽

Eddy Farina ◽

Isabelle Carrard ◽

Jeremy Cela ◽

...

Keyword(s):

Diet Quality ◽

Quantitative Assessment ◽

Construct Validation ◽

Adult Population ◽

The Self ◽

Food Record ◽

Online Questionnaire ◽

Altman Plot ◽

Bland Altman Plot ◽

French Speaking

Several tools assessing diet quality have been developed over the last decades, but their use in public health and clinical practice is limited because they necessitate detailed quantitative assessment of food intake. Our goal was to develop and validate a score (Score d’Alimentation Saine, SCASA) based on a short self-administrated online questionnaire to assess overall diet quality. SCASA targets the adult population in French-speaking Switzerland, but it was designed in a way enabling its adaptation for other regions. The choice of the items involved experts and lay volunteers. Construct validation and inter-method reliability were assessed by screening meal plans and by comparing the self-rated scores with food-record derived scores (kappa and Bland–Altman). SCASA (17 components) discriminated adequately balanced from imbalanced meal plans (93–95% and 44–46% of maximal score). Agreement between self-assessed and food record-based scores ranged between >90% (3 items), 80–89% (3 items), 70–79% (4 items), and <70% (5 items). The Bland–Altman plot showed a mean difference of −1.60 (95% CI −2.36 to −0.84), indicating a slight overestimation of the self-assessed diet quality compared to the food record. SCASA offers a reliable way to assess overall diet quality without requiring burdensome data collection or nutrient calculations.

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