Proliferation and Differentiation of MC3T3-E1 Cells on Calcium Phosphate/Chitosan Coatings

2008 ◽  
Vol 87 (7) ◽  
pp. 650-654 ◽  
Author(s):  
J. Wang ◽  
J. de Boer ◽  
K. de Groot
Keyword(s):  
Calcium Phosphate ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Bone Sialoprotein ◽  
Differentiation Potential ◽  
Collagen Expression ◽  
Significant Difference ◽  
Proliferation And Differentiation ◽  
Proliferative Ability

The incorporation of chitosan into electro-deposited calcium phosphate (CaP) coatings increases bone marrow stromal cell attachment. We hypothesized that such electrodeposited CaP/chitosan coatings can also enhance the proliferative ability and differentiation potential of osteoblasts. To verify this hypothesis, we cultured osteoblast-like MC3T3-E1 cells on these CaP coatings. It was found that MC3T3-E1 cells cultured on the electrodeposited CaP/chitosan coatings had cell proliferation rates higher than those on the electrodeposited CaP coatings. At the same time, both alkaline phosphatase activity and collagen expression were increased, and both bone sialoprotein and osteocalcin genes were up-regulated when MC3T3-E1 cells were cultured on the electrodeposited CaP/chitosan coatings. Additionally, within the range of selected chitosan concentrations in solution, no significant difference was found between the CaP/chitosan coatings. Our results suggest that the electrodeposited CaP/chitosan coatings are favorable to the proliferation and differentiation of MC3T3-E1 cells, which may endow them with great potential for future applications.

Preparation and Characterization of Electrodeposited Calcium Phosphate/Chitosan Coating on Ti6Al4V Plates

2004 ◽  
Vol 83 (4) ◽  
pp. 296-301 ◽  
Author(s):  
J. Wang ◽  
J. de Boer ◽  
K. de Groot
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Carbonate Apatite ◽  
Marrow Stromal Cell ◽  
Electrolytic Coating ◽  
Chitosan Coating ◽  
Significant Difference

Electrolytically deposited carbonate apatite coating demonstrates higher strength but weaker support for bone marrow stromal cell attachment than do biomimetically deposited coatings. It is hypothesized that the incorporation of chitosan will increase the biocompatibility of electrolytic coating while maintaining its original strength. To verify this hypothesis, we formed a hybrid calcium phosphate/chitosan coating through electrodeposition. We found that the incorporation of chitosan influenced calcium phosphate formation and crystallization. Moreover, coating thickness and surface roughness decreased with increasing chitosan concentration. Hybrid coating exhibited an increased dissolution rate in both acidic and neutral simulated physiologic solution, whereas no significant difference on adhesive strength was found between the hybrid and original coatings (P > 0.05). Most importantly, the calcium phosphate/chitosan coating proved to be a more favorable surface for goat bone marrow stromal cell attachment than an unincorporated coating (P < 0.01). Considering its economic and simple production, a hybrid calcium phosphate/chitosan coating is thought to be an attractive candidate for future applications.

In Vitro Characterization of Thermal Behaviour and Bone Marrow Stromal Cell Attachment on Chitosan/Polycaprolactone (CS/PCL) Nanofibrous Scaffolds

2015 ◽  
Vol 7 (11) ◽  
pp. 2427-2435 ◽  
Author(s):  
Mohammed Fayez Al Rez ◽  
H. Fouad ◽  
Ezzedine Laourine ◽  
Martin Hild ◽  
Dilbar Aibibu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Thermal Behaviour ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Marrow Stromal Cell ◽  
Nanofibrous Scaffolds

scholarly journals Development of magnesium calcium phosphate biocement for bone regeneration

2010 ◽  
Vol 7 (49) ◽  
pp. 1171-1180 ◽  
Author(s):  
Junfeng Jia ◽  
Huanjun Zhou ◽  
Jie Wei ◽  
Xin Jiang ◽  
Hong Hua ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Cell Attachment ◽  
Setting Time ◽  
Molar Ratio ◽  
Dihydrogen Phosphate ◽  
Good Ability ◽  
Degradation Ratio ◽  
Close Proximity ◽  
Proliferation And Differentiation ◽  
Fast Dissolution

Magnesium calcium phosphate biocement (MCPB) with rapid-setting characteristics was fabricated by using the mixed powders of magnesium oxide (MgO) and calcium dihydrogen phosphate (Ca(H 2 PO 4 ) 2 ·H 2 O). The results revealed that the MCPB hardened after mixing the powders with water for about 7 min, and the compressive strength reached 43 MPa after setting for 1 h, indicating that the MCPB had a short setting time and high initial mechanical strength. After the acid–base reaction of MCPB containing MgO and Ca(H 2 PO 4 ) 2 ·H 2 O in a molar ratio of 2 : 1, the final hydrated products were Mg 3 (PO 4 ) 2 and Ca 3 (PO 4 ) 2 . The MCPB was degradable in Tris–HCl solution and the degradation ratio was obviously higher than calcium phosphate biocement (CPB) because of its fast dissolution. The attachment and proliferation of the MG 63 cells on the MCPB were significantly enhanced in comparison with CPB, and the alkaline phosphatase activity of MG 63 cells on the MCPB was significantly higher than on the CPB at 7 and 14 days. The MG 63 cells with normal phenotype spread well on the MCPB surfaces, and were attached in close proximity to the substrate, as seen by scanning electron microscopy (SEM). The results demonstrated that the MCPB had a good ability to support cell attachment, proliferation and differentiation, and exhibited good cytocompatibility.

scholarly journals Cellular Performance Comparison of Biomimetic Calcium Phosphate Coating and Alkaline-Treated Titanium Surface

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaohua Yu ◽  
Mei Wei
Keyword(s):  
Calcium Phosphate ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Substrate Surface ◽  
Calcium Phosphate Coating ◽  
Osteoblastic Cell ◽  
Phosphate Coating ◽  
Proliferation And Differentiation ◽  
The Impact

The influence of biomimetic calcium phosphate coating on osteoblasts behaviorin vitrois not well established yet. In this study, we investigated the behavior of osteoblastic rat osteosarcoma 17/2.8 cells (ROS17/2.8) on two groups of biomaterial surfaces: alkaline-treated titanium surface (ATT) and biomimetic calcium phosphate coated ATT (CaP). The cell attachment, proliferation, differentiation, and morphology on these surfaces were extensively evaluated to reveal the impact of substrate surface on osteoblastic cell responses. It was found that the ROS17/2.8 cells cultured on the ATT surface had higher attachment and proliferation rates compared to those on the CaP surface. Our results also showed that the calcium phosphate coatings generated in this work have an inhibiting effect on osteoblast adhesion and further influenced the proliferation and differentiation of osteoblast compared to the ATT surfacein vitro. Cells on the ATT surface also exhibited a higher alkaline phosphatase activity than on the CaP surface after two weeks of culture. Immunofluorescence staining and scanning electron microscopy results showed that the cells adhered and spread faster on the ATT surface than on the CaP surface. These results collectively suggested that substrate surface properties directly influence cell adhesion on different biomaterials, which would result in further influence on the cell proliferation and differentiation.

Re-establishment of Biocompatibility of the In Vitro Contaminated Titanium Surface Using Osteoconductive Powders With Air-Abrasive Treatment

2018 ◽  
Vol 44 (2) ◽  
pp. 94-101 ◽  
Author(s):  
Ceylin S. Tastepe ◽  
Xingnan Lin ◽  
Marcel Donnet ◽  
Behrouz Zandieh Doulabi ◽  
Daniel Wismeijer ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Attachment ◽  
Treatment Modalities ◽  
Dna Amount ◽  
Implant Surface ◽  
Abrasive Treatment ◽  
Significant Difference ◽  
Proliferation And Differentiation ◽  
Film Layer

To achieve re-osseointegration on implant surfaces exposed to peri-implant infections, treatment should re-establish biocompatibility. The aim of this study was to test whether air powder abrasive treatment (APA) using osteoconductive powders can, in addition to cleaning, increase the biocompatibility of the contaminated implant surface. Ninety-six in vitro Ca-precipitated, organic film layer–coated sandblasted and acid-etched titanium discs were treated by APA using erythritol, hydroxylapatite (HA), and biocalcium phosphate (BioCaP) powders (n = 16 per group). Six treatment modalities were created (HA or erythritol cleaning with/without BioCaP coating). MC3T3-E1cells were seeded on discs, and cell attachment, viability, proliferation, and differentiation were evaluated. Pristine discs were used as control (control 1). Contaminated and nontreated discs were used as control (control 2). The cells were stretched and attached in all test groups. The cell viability and proliferation (DNA amount) in all test groups were significantly higher than in the pristine and contaminated disc groups. There was no significant difference between the test groups. The differentiation (alkaline phosphatase activity) of the cells on treated discs was significantly higher than on the contaminated discs but lower than in the pristine group. The cell viability in control 2 was significantly lower than the control 1. The APA with osteoconductive powder on contaminated titanium surfaces promoted the cell viability, proliferation, and differentiation of the MC3T3-E1 cells. The biocompatibility of the surface was higher than that of the contaminated discs. The tested aspects of cell response, with the exception of differentiation, reached to the level of the pristine surface. The in vitro results showed that APA with osteoconductive powders could be a promising method for implant surface treatment.

Effect of Collagen-I Modified Composites on Proliferation and Differentiation of Human Alveolar Osteoblasts

2006 ◽  
Vol 59 (8) ◽  
pp. 571 ◽  
Author(s):  
Yefang Zhou ◽  
Dietmar W. Hutmacher ◽  
Sae-Lim Varawan ◽  
Tit Meng Lim
Keyword(s):  
Protein Adsorption ◽  
Cell Attachment ◽  
Type I ◽  
Mineralized Tissue ◽  
Adsorption Ability ◽  
In Vitro Proliferation ◽  
Matrix Mineralization ◽  
Alp Activity ◽  
Significant Difference ◽  
Proliferation And Differentiation

Collagen modification of scaffolds has been reported to promote matrix mineralization as an effective way to increase osseointegration of implants. The aim of this study was to investigate in vitro proliferation and differentiation of human alveolar osteoblasts (AOs) on medical-grade polycaprolactone–tricalcium phosphate (mPCL-TCP 80:20) scaffolds after collagen modification (mPCL-TCP-c) for 28 days. Collagen modification significantly increased the scaffold’s protein adsorption ability, and improved the initial seeding efficiency and cell attachment at day 1, compared with non-collagen-modified scaffolds. However, the total DNA content of both groups reached similar levels with no significant difference at 28 days’ culture. AOs were observed to spread along the collagen fibres and form extensive collagenous fibres with mineral nodules embedded, while multilayered cell sheets were formed in mPCL-TCP scaffolds. During culture, alkaline phosphatase (ALP) activity increased three- to five-fold in both groups, and collagen modification did not significantly affect either the metabolic rate or ALP activity kinetics of AOs. During osteogenic differentiation, similar gene expression of collagen type-I, osterix, osteopontin, and osteocalcin were detected in both groups. The mPCL-TCP group showed better organized mineralized tissue, but the mPCL-TCP-c showed more scattered and unorganized tissue. These results indicate that collagen modification improved the scaffold’s protein adsorption ability and encouraged initial cell attachment and distribution, but promoted fibrous-like tissue formation rather than mineralized tissue.

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