scholarly journals Increased nerve growth factor expression in the synovial tissues of patients with rotator cuff tears

2021 ◽  
Vol 17 ◽  
pp. 174480692110212
Author(s):  
Ryo Tazawa ◽  
Tomonori Kenmoku ◽  
Kentaro Uchida ◽  
Lars Arendt-Nielsen ◽  
Naoshige Nagura ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rotator Cuff ◽  
Rotator Cuff Tears ◽  
Rotator Interval ◽  
Subacromial Bursa ◽  
Protein Levels ◽  
Patient Groups ◽  
Cox 2 ◽  
Synovial Tissues

Background Rotator cuff tears (RCTs) are often associated with severe shoulder pain. Non-steroidal anti-inflammatory drugs, not recommended for long-term use, do not effectively manage RCT-induced pain, resulting in reduced quality of life. To improve management, a better understanding of the fundamental properties of RCT pain is needed. Here, we aimed to compare the expression levels of nerve growth factor (NGF) and cyclooxygenase-2 (COX-2) mRNA in the synovial tissues of patients with RCT-induced pain and patients with non-painful recurrent shoulder dislocation (RSD). Methods The study included 32 patients with RCT who underwent arthroscopic rotator cuff repair and 28 patients with non-painful RSD who underwent arthroscopic Bankart repair. Synovial tissue samples were harvested from subacromial bursa and rotator interval of RCT patients and from the rotator interval of RSD patients. Samples were analyzed quantitatively expression levels for NGF and COX2 mRNA and NGF protein. Results NGF mRNA and protein levels were significantly higher in the rotator interval of RCT patients than in the rotator interval of RSD patients ( p = 0.0017, p = 0.012, respectively), while COX2 mRNA levels did not differ significantly between the two patient groups. In RCT patients, COX2 mRNA was more highly expressed in the rotator interval than in the subacromial bursa ( p = 0.038), whereas the mRNA and protein levels of NGF did not differ between the two tissues. The expression of NGF mRNA in the synovium of the rotator interval was significantly correlated with the numeric rating scale of pain (ρ = 0.38, p = 0.004). Conclusion NGF mRNA and protein levels were elevated in patients with painful RCT compared with those in patients with non-painful RSD, whereas COX-2 levels were comparable in the two patient groups. These findings provide insights into novel potential strategies for clinical management of RCT.

IL-1β mediates NGF and COX-2 expression through transforming growth factor-activating kinase 1 in subacromial bursa cells derived from rotator cuff tear patients

2019 ◽  
Vol 24 (5) ◽  
pp. 925-929
Author(s):  
Naoshige Nagura ◽  
Kentaro Uchida ◽  
Tomonori Kenmoku ◽  
Gen Inoue ◽  
Mitsufumi Nakawaki ◽  
...  
Keyword(s):  
Growth Factor ◽  
Rotator Cuff ◽  
Rotator Cuff Tear ◽  
Transforming Growth Factor ◽  
Subacromial Bursa ◽  
Cuff Tear ◽  
Cox 2

scholarly journals Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Marie Kjaer ◽  
Wayne Russell ◽  
Peter Schjerling ◽  
Elena Cottarelli ◽  
Kennet N. Christjansen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Breaking Strength ◽  
Target Area ◽  
Type I ◽  
Mrna Levels ◽  
Protein Levels ◽  
Soluble Collagen ◽  
Cox 2 ◽  
Glucagon Like Peptide

Background. The enteroendocrine hormone glucagon-like peptide- (GLP-) 2 is a potent trophic factor in the gastrointestinal tract. The GLP-2 receptor (GLP-2R) is expressed in the stroma of the large bowel wall, which is the major therapeutic target area to prevent anastomotic leakage. We investigated the efficacy of the long-acting GLP-2 analogue ZP1849 on colonic anastomotic wound healing. Methods. Eighty-seven male Wistar rats were stratified into four groups and received daily treatment with vehicle or ZP1849 starting one day before (day -1) end-to-end anastomosis was constructed in the left colon on day 0, and on days 0 (resected colon segment), 3, and 5, gene expressions of GLP-2R, Ki67, insulin-like growth factor- (IGF-) 1, type I (COL1A1) and type III (COL3A1) procollagens, cyclooxygenase- (COX-) 1, COX-2, and matrix metalloproteinase- (MMP-) 7 were quantified by RT-qPCR. Breaking strength, myeloperoxidase (MPO), transforming growth factor- (TGF-) β1, and soluble collagen proteins were measured on days 3 and 5. Results. ZP1849 treatment increased Ki67 (P<0.0001) and IGF-1 (P<0.05) mRNA levels in noninjured colon day 0, and postoperatively in the anastomotic wounds compared to vehicle-treated rats. ZP1849-treated rats had increased (P=0.042) anastomotic breaking strength at day 5 compared with vehicle. COL1A1 and COL3A1 mRNA levels (P<0.0001) and soluble collagen proteins (P<0.05) increased from day 3 to day 5 in ZP1849-treated rats, but not in vehicle-treated rats. COX-2 mRNA and MPO protein levels decreased from day 3 to day 5 (P<0.001) in both groups. ZP1849 treatment reduced TGF-β1 protein levels on day 5 (P<0.001) but did not impact MMP-7 transcription. Conclusions. The GLP-2 analogue ZP1849 increased breaking strength, IGF-1 expression, and cell proliferation, which may be beneficial for colonic anastomotic wound healing.

Limited efficacy of COX-2 inhibitors on nerve growth factor and metalloproteinases expressions in human synovial fibroblasts

2016 ◽  
Vol 21 (3) ◽  
pp. 381-388 ◽  
Author(s):  
Makiko Yorifuji ◽  
Yasunobu Sawaji ◽  
Kenji Endo ◽  
Taiichi Kosaka ◽  
Kengo Yamamoto
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Synovial Fibroblasts ◽  
Nerve Growth ◽  
Limited Efficacy ◽  
Cox 2 ◽  
Cox 2 Inhibitors

Nerve growth factor mRNA and protein levels measured in the same tissue from normal and Alzheimer's disease parietal cortex

1996 ◽  
Vol 42 (1) ◽  
pp. 175-178 ◽  
Author(s):  
Margaret Fahnestock ◽  
Samuel A. Scott ◽  
Nathalie Jetté ◽  
Jean A. Weingartner ◽  
Keith A. Crutcher
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Parietal Cortex ◽  
Nerve Growth ◽  
Protein Levels

scholarly journals Contrast-enhanced MRI of the subdeltoid, subacromial bursa in painful and painless rotator cuff tears

2012 ◽  
Vol 85 (1019) ◽  
pp. 1482-1487 ◽  
Author(s):  
R J Hodgson ◽  
P J O'Connor ◽  
E M A Hensor ◽  
D Barron ◽  
P Robinson
Keyword(s):  
Rotator Cuff ◽  
Rotator Cuff Tears ◽  
Subacromial Bursa ◽  
Enhanced Mri ◽  
Contrast Enhanced ◽  
Contrast Enhanced Mri

Short- and long-term mechanisms of tau regulation in PC12 cells

1995 ◽  
Vol 108 (8) ◽  
pp. 2857-2864 ◽  
Author(s):  
E. Sadot ◽  
J. Barg ◽  
D. Rasouly ◽  
P. Lazarovici ◽  
I. Ginzburg
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Tau Protein ◽  
Immunoblot Analysis ◽  
Term Treatment ◽  
Mrna Levels ◽  
Short Term Treatment ◽  
Nerve Growth ◽  
Protein Levels

Induction by nerve growth factor of neurite outgrowth in PC12 cells is transcription-dependent and is associated with the accumulation of tau protein. It was recently shown that short-term treatment with staurosporine, a protein kinase alkaloid inhibitor, induced an elevation of tau protein levels and outgrowth of stable neurites. In this study, we analyzed the mechanism(s) by which nerve growth factor and staurosporine exert their effects on tau levels. We demonstrate that nerve growth factor affects tau mRNA stability, thus contributing to the observed increase in tau mRNA levels. On the other hand, tau mRNA levels were not affected by the treatment with staurosporine. We also demonstrate that the phosphorylation of tau protein was reduced after treatment of PC12 cells with nerve growth factor or staurosporine, as shown by immunoblot analysis using specific antibodies and alkaline phosphatase treatment. Thus, regulation of tau levels by nerve growth factor appears to be mediated by transcriptional, post-transcriptional and posttranslational steps, whereas the effect of staurosporine on tau levels may be attributed to its effect on the state of phosphorylation of the protein.

Nerve growth factor continuously elevates in a rat rotator cuff tear model

2019 ◽  
Vol 28 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Naoshige Nagura ◽  
Tomonori Kenmoku ◽  
Kentaro Uchida ◽  
Mitsufumi Nakawaki ◽  
Gen Inoue ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rotator Cuff ◽  
Rotator Cuff Tear ◽  
Nerve Growth ◽  
Cuff Tear
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