Hypericum Perforatum L., H. Maculatum Crantz., H. Calycinum L. and H. pulchrum L.: Phytochemical and Morphological Studies

Four species of Hypericum growing in Italy were characterized morphologically and chemically: Hypericum perforatum L., H. maculatum Crantz., H. calycinum L. and H. pulchrum L. The composition of secondary metabolites (phloroglucinols, naphthodianthrones, flavonoids) in the aerial parts of plants collected in different habitats was analysed. The four species show different compositions of phloroglucinols and naphthodianthrones, but there was no qualitative difference in flavonoid content of the species analysed. Study of main-constituent variation during the ontogenetic cycle showed that hypericin decreases and hyperforin increases during the reproductive phase. In St. John's Wort, hypericin and hyperforin are thought to be localised in black nodules. Our investigation shows no clear correlation between either the presence or absence of nodules and hypericin or hyperforin content.

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Medicinally Important Secondary Metabolites and Growth of Hypericum perforatum L. Plants as Affected by Light Quality and Intensity

Environment Control in Biology ◽

10.2525/ecb.45.113 ◽

2007 ◽

Vol 45 (2) ◽

pp. 113-120 ◽

Cited By ~ 13

Author(s):

Tetsuro NISHIMURA ◽

Sayed M. A. ZOBAYED ◽

Toyoki KOZAI ◽

Eiji GOTO

Keyword(s):

Secondary Metabolites ◽

Hypericum Perforatum ◽

Light Quality ◽

Hypericum Perforatum L

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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Red LED light promotes biomass, flowering and secondary metabolites accumulation in hydroponically grown Hypericum perforatum L. (cv. Topas)

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.114239 ◽

2022 ◽

Vol 175 ◽

pp. 114239

Author(s):

Masumeh Karimi ◽

Nima Ahmadi ◽

Morteza Ebrahimi

Keyword(s):

Secondary Metabolites ◽

Hypericum Perforatum ◽

Led Light ◽

Red Led ◽

Hypericum Perforatum L ◽

Hydroponically Grown

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Content variability of bioactive secondary metabolites in Hypericum perforatum L.

Phytochemistry Letters ◽

10.1016/j.phytol.2021.09.011 ◽

2021 ◽

Vol 46 ◽

pp. 71-78

Author(s):

Alessandra Carrubba ◽

Silvia Lazzara ◽

Antonio Giovino ◽

Giuseppe Ruberto ◽

Edoardo Napoli

Keyword(s):

Secondary Metabolites ◽

Hypericum Perforatum ◽

Bioactive Secondary Metabolites ◽

Hypericum Perforatum L

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Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor

Bioresource Technology ◽

10.1016/j.biortech.2011.08.044 ◽

2011 ◽

Vol 102 (21) ◽

pp. 10072-10079 ◽

Cited By ~ 46

Author(s):

Xi-Hua Cui ◽

Hosakatte Niranjana Murthy ◽

You-Xun Jin ◽

Yong-Hyeon Yim ◽

Ji-Yeong Kim ◽

...

Keyword(s):

Secondary Metabolites ◽

Adventitious Root ◽

Root Biomass ◽

Hypericum Perforatum ◽

Airlift Reactor ◽

Hypericum Perforatum L

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Decoupling of Plant Growth and Accumulation of Biologically Active Compounds in Leaves, Roots, and Root Exudates of Hypericum perforatum L. by the Combination of Jasmonate and Far-Red Lighting

Biomolecules ◽

10.3390/biom11091283 ◽

2021 ◽

Vol 11 (9) ◽

pp. 1283 ◽

Cited By ~ 1

Author(s):

Martina Paponov ◽

Manya Antonyan ◽

Rune Slimestad ◽

Ivan A. Paponov

Keyword(s):

Secondary Metabolites ◽

Plant Growth ◽

Root Exudates ◽

Hypericum Perforatum ◽

Secondary Compounds ◽

Biologically Active ◽

Starch Accumulation ◽

Combined Application ◽

Hypericum Perforatum L ◽

Spraying Plants

The plant hormone jasmonic acid (JA) fine tunes the growth–defense dilemma by inhibiting plant growth and stimulating the accumulation of secondary compounds. We investigated the interactions between JA and phytochrome B signaling on growth and the accumulation of selected secondary metabolites in Hypericum perforatum L., a medically important plant, by spraying plants with methyl jasmonate (MeJA) and by adding far-red (FR) lighting. MeJA inhibited plant growth, decreased fructose concentration, and enhanced the accumulation of most secondary metabolites. FR enhanced plant growth and starch accumulation and did not decrease the accumulation of most secondary metabolites. MeJA and FR acted mostly independently with no observable interactions on plant growth or secondary metabolite levels. The accumulation of different compounds (e.g., hypericin, flavonols, flavan-3-ols, and phenolic acid) in shoots, roots, and root exudates showed different responses to the two treatments. These findings indicate that the relationship between growth and secondary compound accumulation is specific and depends on the classes of compounds and/or their organ location. The combined application of MeJA and FR enhanced the accumulation of most secondary compounds without compromising plant growth. Thus, the negative correlations between biomass and the content of secondary compounds predicted by the growth-defense dilemma were overcome.

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Empirical kinetic models for the resinoid extraction from aerial parts of St. John's wort (Hypericum perforatum L.)

Biochemical Engineering Journal ◽

10.1016/j.bej.2008.02.010 ◽

2008 ◽

Vol 41 (1) ◽

pp. 1-11 ◽

Cited By ~ 62

Author(s):

Siniša Kitanović ◽

Dragan Milenović ◽

Vlada B. Veljković

Keyword(s):

Kinetic Models ◽

Hypericum Perforatum ◽

St John’S Wort ◽

Aerial Parts ◽

St John's Wort ◽

Hypericum Perforatum L

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Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v25.1183 ◽

2019 ◽

Vol 25 ◽

pp. 310-315 ◽

Cited By ~ 1

Author(s):

A. G. Salivon ◽

K. V. Lystvan ◽

S. V. Litvinov ◽

S. A. Pchelovska ◽

Yu. V. Shylina ◽

...

Keyword(s):

High Performance ◽

Hypericum Perforatum ◽

Raw Materials ◽

Herbal Medicines ◽

Raw Material ◽

Flavonoid Content ◽

X Ray ◽

Hypericum Perforatum L ◽

Ethanol Extracts ◽

Different Doses

Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical methods for processing and analysis of data. Results. It was shown that acute X-ray pre-sowing irradiation of H. perforatum seeds at a dose of 20 Gy leads to an increase in the crop of medicinal raw material without losing its pharmaceutical value. Irradiation also increases concentration of quercetin, routine, hyperoside in herbal medicines, grown from seeds, that was chronically irradiated with g-radiation in a total dose of 1 Gy. The HPLC analysis of extracts confirmed that the qualitative composition of ethanol extracts of H. perforatum did not change due to the effect of irradiation on the seeds. Conclusions. The obtained results confirm the possibility of application of pre-sowing irradiation of seeds of H. perforatum in the range of 1–35 Gy in order to increase its productivity and increase the pharmaceutical value of the medicinal raw material. Keywords: Hypericum perforatum L., ionizing irradiation, productivity, flavonoids.

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