Investigation of anti proliferative properties and antioxidant activity of aerial parts ethanolic extract of Hypericum perforatum L. by breast cancer 4T1 cell lines

Breast cancer is second most common in women and accounts for 23% of all occurring cancers in women. Patients with breast cancer have increasingly shown resistance and high toxicity to chemotherapeutic drugs. Plant-derived products have proved to be an important source of anti-cancer drugs. The present study was to investigate the anti cancer activity of ethanolic extract of Trachyspermum ammi against MCF-7 cell lines. The preliminary phytochemical studies of ethanolic extract of Trachyspermum ammi showed the presence of flavanoids, alkaloids, glycosides, steroids, carbohydrates, phenols, tannins and terpenes. The IC50 concentration of ethanolic extract of Trachyspermum ammi was determined by MTT assay. The results showed the greater degree of cytotoxicity at the dose of 25µg/ml of Trachyspermum ammi and it has been taken as IC50 value for our further study. Then, we also evaluated the apoptotic effect by measuring the morphological changes, cell viability rates using light and fluorescent microscopical studies and DNA fragmentation by using gel electrophoresis method. The ethanolic extract of Trachyspermum ammi showed significant signs of apoptosis such as cell shrinkage, membrane blebbing and nuclei DNA fragmentation. Further, we analyze the gene expression mRNA levels by using RT-PCR method, it showed the expression of p53 was significantly (P<0.001) increased when compared with normal MCF-7 cell line. The expression of anti apoptotic gene Bcl-2 was significantly (P<0.01) reduced when compared with MCF-7 cell line. From this study we conclude that ethanolic extract of Trachyspermum ammi having significant anticancer activity against MCF-7 cell lines and it might be good therapeutic value for further investigation to develop natural compounds as a anti tumor agents.

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Phenolic content, antioxidant and antifungal activities of acetonic, ethanolic and petroleum ether extracts of Hypericum perforatum L.

Hemijska industrija ◽

10.2298/hemind100819004m ◽

2011 ◽

Vol 65 (2) ◽

pp. 159-164 ◽

Cited By ~ 20

Author(s):

Pavle Maskovic ◽

Jelena Mladenovic ◽

Milica Cvijovic ◽

Gordana Acamovic-Djokovic ◽

Slavica Solujic ◽

...

Keyword(s):

Free Radical ◽

Petroleum Ether ◽

Hypericum Perforatum ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Dry Extract ◽

Hypericum Perforatum L

The objective of this study was to evaluate antifungal and antioxidant activities of Hypericum perforatum L. extracts against the growth of certain fungi. The ethanolic, acetonic and petroleum ether extracts of the plant were evaluated for phenols, flavonoids and non-flavonoids. The highest amounts of phenols (17.6 mg EPC/g dry extract) and flavonoids (16.85 mg EPC/g dry extract) were found in the acetonic extract. The highest inhibitory effect on the growth of Penicillium canescens, Fusarium oxysporum, Alternaria alternata, Aspergillus glaucus and Phialophora fastigiata by the disk diffusion method was exhibited by the ethanolic extract at the concentration of 25 mg/disk. The minimum inhibitory concentration (MIC) of the ethanolic and petroleum ether extracts was 20 mg/mL. The acetonic extract did not affect the growth of the tested fungi. Antioxidant activity was assessed by determining 1,1-Diphenyl-2-picryl-hydrazyl-hydrate (DPPH) free radical scavenging activity. The results showed that the ethanolic extract of Hypericum perforatum L. possesses antioxidant activity. The IC50 values, defined as the concentration of the test sample leading to 50% reduction of the free radical concentration, determined for each measurement were <7.8125, 105.9, 5.99 and 12.77 ?g/ml for the ethanolic extract, the acetonic extract, ascorbic acid and BHT, respectively, for DPPH free radical scavenging activity.

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Empirical kinetic models for the resinoid extraction from aerial parts of St. John's wort (Hypericum perforatum L.)

Biochemical Engineering Journal ◽

10.1016/j.bej.2008.02.010 ◽

2008 ◽

Vol 41 (1) ◽

pp. 1-11 ◽

Cited By ~ 62

Author(s):

Siniša Kitanović ◽

Dragan Milenović ◽

Vlada B. Veljković

Keyword(s):

Kinetic Models ◽

Hypericum Perforatum ◽

St John’S Wort ◽

Aerial Parts ◽

St John's Wort ◽

Hypericum Perforatum L

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Biotinylated xanthohumol: synthesis and in vitro biological evaluation for anticancer therapy

10.20944/preprints201807.0039.v1 ◽

2018 ◽

Author(s):

Monika Stompor ◽

Rafał Podgórski ◽

Marta Świtalska ◽

Joanna Wietrzyk

Keyword(s):

Breast Cancer ◽

Antioxidant Activity ◽

Cancer Cells ◽

Cell Lines ◽

Biological Evaluation ◽

Breast Cancer Cell Lines ◽

Dpph Method ◽

Ic50 Values ◽

Mcf 7

Two biotinylated derivatives of the main hop chalcone xanthohumol (1) were prepared by a one-step synthesis via esterification using biotin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC HCl) and 4-dimethylaminopyridine (DMAP) as coupling reagents. The products were characterized spectroscopically and their antiproliferative activity toward MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1 and Balb/3T3 cell lines was investigated using the SRB assay. For all three tested compounds the best activity was noted in the case of human (MCF-7) and mice (4T1) breast cancer cell lines (IC50 values < 9 μM). Both biotinylated derivatives showed higher anticancer activity than xanthohumol (1) towards all types of tested breast cancer cells. Double biotinylated xanthohumol (3) proved to be the most active in inhibiting cell growth, with IC50 values equal to 5.35 ± 1.5 μM for 4T1 and 8.03 ± 0.53 µM for MCF-7 cell lines. Compound 3 was also more active than 1 and 2 against liver cancer cells HepG2 (IC50 = 17.37 ± 5.1 μM), while the IC50 values for 1 and 2 were equal to 21.5 ± 2.7 and 22.1 ± 3.9 µM, respectively. 4‑O‑biotinylxanthohumol (2) was the second most active growth inhibitor, particularly with respect to MCF-7 (IC50 = 6.19 ± 1.7 μM) and 4T1 (IC50 = 6.64 ± 0.4 μM) cell lines. The antioxidant activity was evaluated using the 1.1-diphenyl-2-picrylhydrazyl radical (DPPH) method. All tested compounds (1-3) have antioxidant activity between 2.73 and 3.38 mM. It was reported for the first time that new prenylated chalcones containing the biotin moiety effectively inhibited proliferation of cancer cells in vitro.

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Stryphnodendron adstringens (“Barbatimão”) Leaf Fraction: Chemical Characterization, Antioxidant Activity, and Cytotoxicity Towards Human Breast Cancer Cell Lines

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-017-2632-z ◽

2017 ◽

Vol 184 (4) ◽

pp. 1375-1389 ◽

Cited By ~ 7

Author(s):

A. P. L. Sabino ◽

L. M. S. Eustáquio ◽

A. C. F. Miranda ◽

C. Biojone ◽

T. N. Mariosa ◽

...

Keyword(s):

Breast Cancer ◽

Antioxidant Activity ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Human Breast Cancer ◽

Chemical Characterization ◽

Human Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Human Breast

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Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts ofPothomorphe umbellataL. (Piperaceae)

BioMed Research International ◽

10.1155/2013/206581 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 13

Author(s):

Andrey P. Lopes ◽

Bianca S. Bagatela ◽

Paulo C. P. Rosa ◽

Dhammika N. P. Nanayakkara ◽

José Carlos Tavares Carvalho ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methylene Chloride ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Major Constituent ◽

Pharmacokinetic Parameters ◽

Active Constituent ◽

Cytotoxic Effects ◽

Aerial Parts

The crude ethanolic extract from aerial parts ofPothomorphe umbellataL. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50=1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50=4.5 μg/mL), methylene chloride (IC50=5.9 μg/mL), ethyl acetate (IC50=8.0 μg/mL), 4-nerolidylcatechol (IC50=8.6 μg/mL), and the sterol fractions (IC50>12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50=0.4 μg/mL) and methylene chloride fraction (IC50=2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of ano-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective.

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