scholarly journals Phytochemical Analysis and Antiradical Properties of Sarcodon imbricatus (L.:Fr) Karsten

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Maria Carla Marcotullio ◽  
Gildas Norbert Oball-Mond Mwankie ◽  
Lina Cossignani ◽  
Bruno Tirillini ◽  
Rita Pagiotti
Keyword(s):  
Antiradical Activity ◽  
Unsaturated Fatty Acids ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Phytochemical Analysis ◽  
Ergosterol Peroxide ◽  
Antiradical Properties ◽  
Dpph Radical Scavenging ◽  
Good Food

The chemical composition of Sarcodon imbricatus (L.:Fr) Karsten (Hydnaceae) was evaluated to assess it as source of nutrients and nutraceuticals. The analyzed mushroom contains ergosterol and ergosterol peroxide. S. imbricatus methanolic extract showed a moderate antiradical activity (measured by DPPH radical scavenging activity). The combination of bioactive compounds and rich nutritional composition (high contents in protein, low content in fat, and its content of unsaturated fatty acids) makes the mushroom a good food.

scholarly journals Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of Hydnocarpus pentandra (Buch.-Ham.) Oken

2017 ◽  
Vol 9 (4) ◽  
pp. 576
Author(s):  
Prashith Kekuda TR ◽  
Dunkana Negussa Kenie ◽  
Chetan DM ◽  
Raghavendra L Hallur
Keyword(s):  
Leaf Extract ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Process ◽  
In Vitro Assays ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Bioactive Agents

<p><strong>Objectives</strong>: The present study was conducted to evaluate antimicrobial, insecticidal and radical scavenging activity of leaf extract of <em>Hydnocarpus pentandra</em> (Buch.-Ham.) Oken belonging to the family Achariaceae.</p><p><strong>Methods</strong>: Extraction process of shade dried and powdered leaf was carried out by maceration technique. Extract was screened for phytochemicals by standard tests. Antibacterial and antifungal activity of leaf extract was determined by Agar well diffusion and Poisoned food technique respectively. Antiradical activity of leaf extract was evaluated by two in vitro assays namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Insecticidal activity of leaf extract was determined against II instar and IV instar larvae of <em>Aedes aegypti</em>.</p><p><strong>Results</strong>: Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, saponins, glycosides, triterpenes and steroids in the leaf extract. Leaf extract exhibited marked inhibitory activity against Gram positive bacteria when compared to Gram negative bacteria. <em>Bacillus cereus</em> (zone of inhibition 1.86±0.05cm) and <em>Escherichia coli</em> (zone of inhibition 1.06±0.05cm) were inhibited to highest and least extent respectively. Extract was effective in inhibiting mycelial growth of seed-borne fungi. Among fungi, the susceptibility to extract was in the order: <em>Curvularia</em> sp. (53.64% inhibition) &gt; <em>Fusarium</em> sp. (45.81% inhibition) &gt; <em>Alternaria</em> sp. (35.08% inhibition). The extract exhibited concentration dependent larvicidal activity with marked activity being observed against II instar larvae (LC<sub>50</sub> value 0.79mg/ml) when compared to IV instar larvae (LC<sub>50</sub> value 1.37mg/ml). Leaf extract scavenged DPPH and ABTS radicals dose dependently with an IC<sub>50</sub> value of 13.91µg/ml and 6.03µg/ml respectively.</p><p><strong>Conclusions</strong>: The plant is shown to be an important source of bioactive agents. The observed bioactivities could be attributed to the phytochemicals present in the leaf extract. Further studies on characterization and bioactivity determination of isolated components from leaf extract are to be carried out.</p>

scholarly journals DPPH-Scavenging Activities and Structure-Activity Relationships of Phenolic Compounds

2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Cheng-Dong Zheng ◽  
Gang Li ◽  
Hu-Qiang Li ◽  
Xiao-Jing Xu ◽  
Jin-Ming Gao ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Basic Structure ◽  
Hydroxyl Group ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

Thirty-eight phenolic compounds (including 31 flavonoids) were examined for their DPPH radical-scavenging activities, and structure-activity relationships were evaluated. Specifically, the presence of an Ortho-dihydroxyl structure in phenolics is largely responsible for their excellent antiradical activity. 3-Hydroxyl was also essential to generate a high radical-scavenging activity. An increasing number of hydroxyls on flavones with a 3′,4′-dihydroxyl basic structure, the presence of a third hydroxyl group at C-5′, a phloroglucinol structure, glycosylation and methylation of the hydroxyls, and some other hydroxyls, for example 5-, and 7-hydroxyl in ring A, decreased the radical-scavenging activities of flavonoids and other phenolics.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

2017 ◽  
pp. 59-63
Author(s):  
Abdul Sadat ◽  
Mayukh Hore ◽  
Kaushik Chakraborty ◽  
Subhrajyoti Roy
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

scholarly journals Evaluation of Antiradical Activity Grape Seeds and Olive (Olea europaea) Pits Extracts

2013 ◽  
Vol 6 (1) ◽  
pp. 185-190
Author(s):  
F. Tajalli
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Maximum Activity ◽  
Fruit Juices ◽  
Scavenging Activity ◽  
Grape Seeds ◽  
Antiradical Properties ◽  
Environmental Treatment ◽  
Dpph Method

The antiradical activity of grape seeds and olive pits extracts were investigated. The aim of this study is evaluating the radical scavenging activity of methanol extracts of olive pits and grape seeds and to recover a functional and antiradical compound from olive oil and fruit juices factories waste and also for environmental treatment. The antiradical properties of ripe and unripe olive pits (RIOP and URIOP) and grape seeds (IGS) that are respectively used in Iranian oil industries and fruit juices producers are examined. All seeds and pits extracts showed DPPH radical scavenging activity ranging from 24.51 to 97.06. For this purpose a methanolic extract was prepared from each of the RIOP, URIOP and IGS and their radical scavenging ability is determined with DPPH method. For this trial the effect of 3 different dilutions (100, 200 and 300 ?g/L) of RIOP, URIOP, IGS extracts was used, separately. It was appeared that in above 100 ppm concentrations the antiradical properties reaches to its maximum activity. Also, IGS extract shows better effects in 100 ppm concentrations in comparison with RIOP, URIOP and ascorbic acid. The study shows that grape seeds can be used as a rich source of functional and antiradical compound and anticancer drugs production. Keywords: Iranian olive pits; DPPH; Antiradical activity; Radical scavenging; Grape seeds.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.  doi: http://dx.doi.org/10.3329/jsr.v6i1.16654 J. Sci. Res. 6 (1), 185-190 (2014)

scholarly journals Phytochemical Constituent and Antioxidant Activity of Thaumatococcus daniellii Benn (Benth.) Leaves (Food Wrapper)

2016 ◽  
Vol 2 ◽  
pp. 55-61
Author(s):  
Oke Ibukunoluwa Ayodeji ◽  
Olufemi Adeleye ◽  
Olugbenga Dada ◽  
Olaoluwa Adeyemi ◽  
Godswill N. Anyasor
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Ferric Ion ◽  
Aqueous Fraction ◽  
Standard Procedures ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent

Thaumatoccocus daniellii Benn (Benth.) is an organic food wrapper that influences the color and flavour of most foods processed and packaged with it. Hence, this study was designed to evaluate the phytochemical constituent and antioxidant activity of T. daniellii leave. Quantitative phytochemical analysis was carried out using standard procedures while antioxidant activity was assayed using 2’,2’diphenylpicrylhydrazine (DPPH) and ferric ion reducing antioxidant potential (FRAP). Results showed that T. daniellii leaves contained flavonoids, polyphenols, alkaloids and saponins. Furthermore, aqueous leaf fraction had higher quantity of polyphenols (0.41 ± 0.1 mg% gallic acid) and flavonoids (0.28 ± 0.1 mg% quercetin) than polyphenols (0.23 ± 0.3 mg% gallic acid) and flavonoids (0.11 ± 0.1 mg% quercetin) in hexane leaf fraction. Investigation of antioxidant activity revealed that 100 - 500 mg/ml aqueous leaf fraction exhibited a significantly (P<0.05) higher DPPH radical scavenging activity than the hexane leaf fraction. In addition, the aqueous fraction of T. daniellii leaves exhibited a significantly (P<0.05) higher ferric ion reducing potential than hexane leaf fraction. Thus, data from this study indicated that T. daniellii leaves color and flavour enhancing properties could be attributed to the presence of polyphenols and flavonoids. In addition, its use as food wrapper may introduce phyto-antioxidants into foods processed and packaged with T. daniellii leaves.

scholarly journals Antioxidant and antiradical activity of ferulates

2011 ◽  
Vol 23 (No. 2) ◽  
pp. 64-68 ◽  
Author(s):  
M. Karamać ◽  
A. Buciński ◽  
B. Pegg R ◽  
R. Amarowicz
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging ◽  
Beta Carotene ◽  
Hydroxyl Group ◽  
Dpph Radical ◽  
Meta Position ◽  
Antiradical Properties ◽  
Isoferulic Acid ◽  
Methyl Ferulate ◽  
Dpph Radical Scavenging

Antioxidant and antiradical activities of ferulates (i.e., ferulic acid, isoferulic acid, coniferyl aldehyde, and methyl ferulate) were investigated using a &beta;-carotene-linoleate model system and a DPPH radical scavenging assay, respectively. Compounds so tested exhibited antioxidant and antiradical properties to varying degrees. Methyl ferulate showed the strongest antioxidant activity, whereas the parent phenolic acid was the most active ferulate to scavenge the DPPH radical (DPPH<sup>&middot;</sup>). Isoferulic acid at concentrations ranging from 10 to 100 nmol/assay did not impart an antiradical efficacy; this may be attributed to the location of the hydroxyl group in the meta position on the aromatic ring. &nbsp;

scholarly journals DPPH Radical Scavenging Assay, Biological Activities, Nutritional Composition and Quality Parameters of Momordica charantia Seeds Grown in District Charsadda, KPK, Pakistan

2017 ◽  
Vol 60 (2) ◽  
pp. 80-86
Author(s):  
Fazia Ghaffar ◽  
Bushra Kainat ◽  
Hamid Ullah Shah ◽  
Inayat Ur Rahman
Keyword(s):  
Unsaturated Fatty Acids ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Momordica Charantia ◽  
Omega 3 ◽  
Crude Fibre ◽  
Local Variety ◽  
Seed Cake

The nutritional composition, physicochemical characteristics, antimicrobial activity and DPPHfree radical scavenging activity of the oil extracted and proximate and elemental composition of seed cakeof the local variety of Momordica charantia were examined as per AOAC or elsewhere mentionedprocedures. Physicochemical properties of the oil exhibited colour (1.71 R/U, 1.19 Y/U) being reddishbrown, the higher iodine value (105.5), saponification value (190.7), low acid value (1.3) and the higherproportion of unsaturated fatty acids (especially the omega-3 fatty acid) as compared to saturated fattyacids of the oil are an indication of its edibility and industrial utility. The oil also showed good inhibitoryaction against the selected fungal and bacterial strains as well as DPPH free radical scavenging activity.Results of the proximate composition of the seed cake showed it to be good source of total proteins (18.17%), crude fibre 10.37%, percent oil (38.1%) and elements such as calcium (374.9 mg/100 g), copper(3.01 mg/100 g), iron (39.57 mg/100 g) and zinc (12.04 mg/100 g). It can be concluded from the studythat oil and seed cake of the local variety of bitter gourd seed (BGS) oil possesses a good nutritional,antioxidant and antimicrobial potential.

scholarly journals GC-MS profiling and DPPH radical scavenging activity of the bark of Tampoi (Baccaurea macrocarpa)

F1000Research ◽  
2019 ◽  
Vol 7 ◽  
pp. 1977
Author(s):  
Erwin Erwin ◽  
Widar Ristiyani Pusparohmana ◽  
Indah Permata Sari ◽  
Rita Hairani ◽  
Usman Usman
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
East Kalimantan ◽  
Dpph Radical Scavenging

Background : Tampoi ( Baccaurea macrocarpa) is a tropical rainforest plant that produces edible fruit and is native to Southeast Asia, especially East Kalimantan, Indonesia. Previous research showed that Tampoi potentially can be developed as a drug. It was reported that the extract of Tampoi fruit displayed antioxidant activity, which was correlated with its phenolic and flavonoid substances. There is no information about the antioxidant activity of other parts of this plant, such as the bark, which might also have this kind of activity. Therefore, the aim of this study was to evaluate the phytochemical using GC-MS analysis, toxicity againt Artemia salina, and antioxidant activity with DPPH radical scavenging method of the bark of Tampoi. Methods : The bark of Tampoi was extracted with methanol and concentrated using rotary evaporator to obtain the methanol extract of the bark. Secondary metabolites of this extract was determined using phytochemical analysis. Afterward, the methanol extract was tested for its toxicity using brine shrimp lethality test and antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method. Results : Phytochemical evaluation results showed that the methanol extract of bark of this plant contains several secondary metabolites including alkaloids, flavonoids, phenolics, steroids, and triterpenoids. The toxicity test displayed no toxic property due to a LC 50 value above 1000 ppm. For antioxidant activity, the result exhibited that the methanol extract of bark of this plant could be categorized as an active extract with IC 50 value of 11.15 ppm. Moreover, based on gas chromatography-mass spectrometer analysis, there are 37 isolated compounds from the bark, one of which is methylparaben, a phenolic predicted to act as an antioxidant. Conclusion: The results obtained in this research demonstrated that the bark of Tampoi (B. macrocarpa) has potential as an antioxidant.

Phytochemical Analysis, Antibacterial and Antioxidant Potential of Marine Red Seaweed Caulacanthusustulatus

2019 ◽  
Vol 12 (6) ◽  
pp. 4701-4708
Author(s):  
Mohideen Askar Nawas P ◽  
Sujatha Ravi
Keyword(s):  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Red Seaweed ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Hydroxyl Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Marine algae are known to produce a wide variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. The marine red seaweed Caulacanthusustulatus, collected from the Kilakarai region located between (9.23135° N, 78.7844° E) Ramanathapuram District, Tamil Nadu, India. The seaweed extract was prepared from various solvent extracts namely aqueous, ethanol, methanol and acetone was tested for their phytochemical analysis, antibacterial activity against human pathogens viz., Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and aquatic pathogens viz., Aeromonas hydrophila and Vibrio vulnificus using disc diffusion methods and in vitro antioxidant activity such as DPPH radical scavenging activity, ABTS radical scavenging activity and Hydroxyl radical scavenging activity of selected solvent extracts. Phytochemical analysis of sixteen different chemical compounds was carried out. The maximum nine phytochemical compounds were present in the methanol and ethanol extracts and the minimum six compounds were present in aqueous extract. The highest antibacterial activity was present in human pathogen P. aeruginosa (20.66 ± 1.1mm) likewise the aquatic pathogen the antibacterial activity was increase in Aeromonas hydrophila (20.33 ± 1.5mm). The methanol extract was significantly higher in DPPH radical scavenging activity (67.07 ± 0.5μg/ml), ABTS radical scavenging activity (72.4 ± 0.6 μg/ml) and Hydroxyl radical scavenging activity (54.06 ± 0.58μg/ml). This study indicates the potential use of red seaweed; in particularC.ustulatus extracts are treating human and aquatic bacterial pathogens and it could be a potential candidate for the natural compounds as antioxidant.

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