scholarly journals Evaluation of Antiradical Activity Grape Seeds and Olive (Olea europaea) Pits Extracts

2013 ◽  
Vol 6 (1) ◽  
pp. 185-190
Author(s):  
F. Tajalli
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Maximum Activity ◽  
Fruit Juices ◽  
Scavenging Activity ◽  
Grape Seeds ◽  
Antiradical Properties ◽  
Environmental Treatment ◽  
Dpph Method

The antiradical activity of grape seeds and olive pits extracts were investigated. The aim of this study is evaluating the radical scavenging activity of methanol extracts of olive pits and grape seeds and to recover a functional and antiradical compound from olive oil and fruit juices factories waste and also for environmental treatment. The antiradical properties of ripe and unripe olive pits (RIOP and URIOP) and grape seeds (IGS) that are respectively used in Iranian oil industries and fruit juices producers are examined. All seeds and pits extracts showed DPPH radical scavenging activity ranging from 24.51 to 97.06. For this purpose a methanolic extract was prepared from each of the RIOP, URIOP and IGS and their radical scavenging ability is determined with DPPH method. For this trial the effect of 3 different dilutions (100, 200 and 300 ?g/L) of RIOP, URIOP, IGS extracts was used, separately. It was appeared that in above 100 ppm concentrations the antiradical properties reaches to its maximum activity. Also, IGS extract shows better effects in 100 ppm concentrations in comparison with RIOP, URIOP and ascorbic acid. The study shows that grape seeds can be used as a rich source of functional and antiradical compound and anticancer drugs production. Keywords: Iranian olive pits; DPPH; Antiradical activity; Radical scavenging; Grape seeds.  © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.  doi: http://dx.doi.org/10.3329/jsr.v6i1.16654 J. Sci. Res. 6 (1), 185-190 (2014)

scholarly journals Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family

2013 ◽  
Vol 4 (1) ◽  
pp. 457
Author(s):  
Muhammad Da'i ◽  
Didiek Setiawan ◽  
Rosita Melannisa
Keyword(s):  
Phenolic Compound ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenolic Compound ◽  
Dpph Method

Several studies show that some plants and fruits could protect human body from free radical danger exposure. Zingiberaceae family has some chemical substances that have antiradical activity, such as phenolic compound and flavonoid. The aim of this study is to determine the correlation of total phenolic compound towards its antiradical activity. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). The total phenolic compound is determined by spectrofotometry using Folin-Ciocalteu reagent and counted as GAE (Gallic Acid Equivalent). Based on this study, the total phenolic concentration of ethanolic extract of jahe (Zingiber officinale (L.) Rosc), kencur (Kaemferia galanga L.), lengkuas (Languas galanga), lempuyang gajah (Zingiber littorale) and lempuyang pahit (Zingiber americana) is 73.99 mg/g; 23.848 mg/g; 37.841 mg/g; 49.725 mg/g and 40.802 mg/g respectively. The radical scavenging activity (IC50) of jahe (27.38 µg/mL), lengkuas (93,27 µg/mL), lempuyang pahit (105,08 µg/mL), meanwhile lempuyang gajah and kencur show low activity of radical scavenging.Keywords: Zingiberaceae, total phenolic compound, antiradical, DPPH

scholarly journals Free Radical Scavenging Activity of Synthetic and Naturally Occurring Polyhydroxy-, Aminohydroxynaphthazarins and Related Compounds

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Nikita S. Polonik ◽  
Yuri E. Sabutskii ◽  
Sergey G. Polonik
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Naturally Occurring ◽  
Antiradical Properties ◽  
Relationship Of ◽  
First Time ◽  
Related Compounds

Fifty natural and synthetic hydroxy-, aminohydroxynaphthazarins and related quinones were tested for their antiradical activities using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) scavenging assay. The main features of structure-antiradical activity relationship of amino- and hydroxyquinones were determined for the first time. It was found that naphthazarins (5,8-dihydroxy-1,4-naphthoquinones) are much more active against ABTS-radical than related 1,4-naphthoquinones and 5-hydroxy-1,4-naphthoquinones. Natural aminonaphthazarins (spinamine E, echinamine A) and polyhydroxylated naphthazarins (spinazarin, echinochrome, spinochrome D and E) revealed high antiradical properties. In most cases, the presence of long chained O-alkoxy substituent in benzenoid part of naphthazarin core enhanced their antiradical activity.

Note: A Comparative Study on the in Vitro Antiradical Activity and Hydroxyl Free Radical Scavenging Activity in Aged Red Wines

2003 ◽  
Vol 9 (6) ◽  
pp. 383-387 ◽  
Author(s):  
P. Kefalas ◽  
S. Kallithraka ◽  
I. Parejo ◽  
D. P. Makris
Keyword(s):  
Free Radical ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Red Wines ◽  
Hydroxyl Free Radical ◽  
Important Relationship

The antiradical and hydroxyl free radical scavenging activities were estimated in twenty-five, aged red wines from different areas in Greece. The antiradical activity (AAR) was determined by means of the wellknown DPPH• method, and its values ranged from 24.7 to 125.1. A novel, chemiluminescence-based, highly sensitive assay was applied for determination of the hydroxyl free radical scavenging activity (SAHFR), which varied from 1.62 to 12.22 mM quercetin equivalents. The values from the two assays correlated very well (r2 =0.8542, P<0.001), which confirmed an important relationship between SAHFR and AAR. This tendency in aged red wines, which may be significant in evaluating the antioxidant behaviour of red wine polyphenols, is discussed on the basis of previous research and relevant data.

Assay-Guided Extraction and Kinetics of Radical Scavenging Activity of Curcumin from the Rhizome of Curcuma longa Linn

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Jubril Olayinka Akolade ◽  
Hussein O.B. Oloyede ◽  
Amuzat Olalekan Aliyu ◽  
Sarah Abimbola Akande ◽  
Aderoumu Ibrahim Ganiyu ◽  
...  
Keyword(s):  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Weight Ratio ◽  
Pharmaceutical Products ◽  
Appreciable Amount ◽  
Absolute Methanol ◽  
Second Phase ◽  
Scavenging Activity

Extraction of phytoceuticals from medicinal plants need to be optimized to produce standardized, dose-dependent and reliable extracts to meet food or drug administration regulations for translation into approved nutraceutical or pharmaceutical products. Effect of selected limiting extraction variables on yield and antiradical activity of curcumin-rich extract from Curcuma longa rhizomes was investigated. Assay-guided response (free radical scavenging activity) was used to determine the optimized set of extraction parameters. Curcumin-rich extract was produced using solvent boiling process at 65 oC with absolute methanol of weight ratio 1:50 (w/v) within 1 h. Relative radical scavenging activity of the extract derived using the optimized set of parameters recorded an IC50 of 497 μg/ml and its total antiradical capacity determined within 6 h (76 - 85 % at a concentration of 400 – 600 μg/ml) was not significantly different from that of commercially available natural curcumin used as reference. Time-dependent kinetic analysis revealed an initial fast burst in rate of activity (2.27 – 7.77 min-1) followed by a slow reaction rate in a steady second phase (0.12 – 0.54 min-1). Conclusively, appreciable amount of curcumin-rich extract of enhanced antiradical activity was extracted from the rhizome of C. longa using assay-guided procedure.

scholarly journals Bioprocessing of Squid Pens Waste into Chitosanase by Paenibacillus sp. TKU047 and Its Application in Low-Molecular Weight Chitosan Oligosaccharides Production

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1163 ◽  
Author(s):  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
Van Bon Nguyen ◽  
Trung Dung Tran ◽  
Anh Dzung Nguyen ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Radical Scavenging ◽  
Maximum Activity ◽  
Degree Of Polymerization ◽  
Chitosan Oligosaccharide ◽  
Scavenging Activity ◽  
Paenibacillus Sp

Chitosan oligosaccharide (COS) has become of great interest in recent years because of its worthy biological activities. This study aims to produce COS using the enzymatic method, and investigates Paenibacillus sp. TKU047, a chitinolytic-producing strain, in terms of its chitosanase productivity on several chitinous material-containing mediums from fishery process wastes. The highest amount of chitosanase was produced on the medium using 2% (w/v) squid pens powder (0.60 U/mL) as the single carbon and nitrogen (C/N) source. The molecular mass of TKU047 chitosanase, which could be the smallest one among chitinases/chitosanases from the Paenibacillus genus, was approximately 23 kDa according to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. TKU047 chitosanase possessed the highest activity at 60 °C, pH 7, and toward chitosan solution with a higher degree of deacetylation (DDA) value. Additionally, the hydrolysis products of 98% DDA chitosan catalyzed by TKU047 chitosanase showed the degree of polymerization (DP) ranging from 2 to 9, suggesting that it was an endo-type activity chitosanase. The free radical scavenging activity of the obtained chitosan oligosaccharide (COS) was determined. The result showed that COS produced with Paenibacillus sp. TKU047 chitosanase expressed a higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than that from the commercial COSs with maximum activity and IC50 values of 81.20% and 1.02 mg/mL; 18.63% and 15.37 mg/mL; and 15.96% and 15.16 mg/mL, respectively. As such, Paenibacillus sp. TKU047 may have potential use in converting squid pens waste to produce chitosanase as an enzyme for bio-activity COS preparation.

scholarly journals Mangifera indica Bark Essential Oil: Chemical Composition and Biological Activities in Comparison with Aqueous and Ethanol Extracts

2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Gislaine Aurelie Kemegne ◽  
Maximilienne Ascension Nyegue ◽  
Sylvain Leroy Sado Kamdem ◽  
François-Xavier Etoa ◽  
Chantal Menut
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Mangifera Indica ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Dpph Method ◽  
Ethanol Extracts

Ethnobotanic surveys have revealed the use of Mangifera indica L. (Anacardiaceae) bark for the treatment of infectious diarrhea. The essential oil of M. indica bark is described for the first time for its chemical composition, radical scavenging activity (DPPH method) and antimicrobial properties. The total phenols content of its water and ethanol bark extracts as well as their radical scavenging and antimicrobial properties were also evaluated. Four commercial plant extracts were also studied for a comparison purpose. The antimicrobial activities were measured for all samples against three Gram (-): Escherichia coli, Salmonella enteritidis, Shigella and two Gram (+): Staphylococcus aureus and Bacillus cereus bacteria. The M. indica bark essential oil was characterized by the association of two major sesquiterpenes: ( E)-β-caryophyllene (60.3%) and α-humulene (36.7%). It presented the lowest ratio of concentration to inhibition zone diameter on all the strains. The aqueous and ethanol extracts of M. indica bark were characterized by high contents of total phenols compounds and high radical scavenging activity compared to the essential oil. Finally, the interesting combination of the antibacterial and antiradical activities of the aqueous M. indica bark extract justifies the traditional use of this plant part in decoction form for the treatment of diarrheal infections.

scholarly journals Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

Toxins ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 748
Author(s):  
Neda Alvarez-Ortega ◽  
Karina Caballero-Gallardo ◽  
María Taboada-Alquerque ◽  
Jackeline Franco ◽  
Elena E. Stashenko ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Neuroblastoma Cells ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Human Neuroblastoma ◽  
Dependent Relationship ◽  
Dpph Method ◽  
Hydroethanolic Extract

Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (p < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.

scholarly journals DEVELOPMENT AND EVALUATION OF HERBAL ANTIAGING CREAM FORMULATION CONTAINING ANNONA SQUAMOSA LEAF EXTRACT

2019 ◽  
pp. 210-214
Author(s):  
VANDANA MAHAWAR ◽  
KALPANA PATIDAR ◽  
NEELAM JOSHI
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Annona Squamosa ◽  
Dpph Method ◽  
Dpph Scavenging

Objective: The aim of this research article is to develop and evaluate herbal antiaging cream using Annona squamosa leaf extract because of its antioxidant potential. Method: Free radical scavenging activity of Annona squamosa aqueous was determined by DPPH method. 0.5 ml of each solution of concentration of sample was added to 3 ml of 0.004% ethanolic DPPH free radical solution. After 30 minutes the absorbance of the preparations were taken at 517 nm by a UV spectrophotometer which was compared with the corresponding absorbance of standard ascorbic acid. The qualitative phytochemical analysis of Annona squamosa (custard apple) leaf extract shows the presence of flavonoids, tannins, alkaloids and phenols and absence of terpenoids and steroids Results: Formulation A3 shows good DPPH scavenging activity as compare to formulation A1 and A2 and ascorbic acid. Formulation A3 is stable for 3 months. Conclusion: Due to high antioxidant values of Annona squamosa it is concluded that it is possible to develop anti-aging cream using aqueous extract of Annona squamosa leaf.

scholarly journals Phytochemical Analysis and Antiradical Properties of Sarcodon imbricatus (L.:Fr) Karsten

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Maria Carla Marcotullio ◽  
Gildas Norbert Oball-Mond Mwankie ◽  
Lina Cossignani ◽  
Bruno Tirillini ◽  
Rita Pagiotti
Keyword(s):  
Antiradical Activity ◽  
Unsaturated Fatty Acids ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Phytochemical Analysis ◽  
Ergosterol Peroxide ◽  
Antiradical Properties ◽  
Dpph Radical Scavenging ◽  
Good Food

The chemical composition of Sarcodon imbricatus (L.:Fr) Karsten (Hydnaceae) was evaluated to assess it as source of nutrients and nutraceuticals. The analyzed mushroom contains ergosterol and ergosterol peroxide. S. imbricatus methanolic extract showed a moderate antiradical activity (measured by DPPH radical scavenging activity). The combination of bioactive compounds and rich nutritional composition (high contents in protein, low content in fat, and its content of unsaturated fatty acids) makes the mushroom a good food.

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