scholarly journals Ultrasonically Assisted Extraction of Total Phenols and Flavonoids from Rhodiola Rosea

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Jordanka Staneva ◽  
Milka Todorova ◽  
Neyko Neykov ◽  
Ljuba Evstatieva

This work deals with ultrasonically assisted extraction (UAE) of biologically active compounds from rhizomes of Rhodiola rosea, a popular medicinal plant. The influence of temperature, type of solvent and solid/solvent ratio on the yield of total extracts, total phenols and flavonoids was established. The best extraction of total phenols and flavonoids was achieved by using 50% aqueous EtOH and MeOH, respectively. Five times increase of solid/solvent ratio (from 1:20 to 1:100 (w/v)) leads to slow increase of the yield of total phenols and flavonoids. The extraction effectiveness of conventional maceration with 50% EtOH and UAE performed for 1 h at 25°C using the same solvent with respect of total phenols was comparable.

2017 ◽  
Vol 32 (4) ◽  
pp. 625-630 ◽  
Author(s):  
Devashan Naidoo ◽  
Lenka Poštová Slavětínská ◽  
Adeyemi O. Aremu ◽  
Jiri Gruz ◽  
Ondrej Biba ◽  
...  

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Antoaneta Trendafilova ◽  
Milka Todorova

The effect of ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and conventional extraction (CE) on the extraction of biologically active compounds from Achillea millefolium Proa has been investigated. The extracts were analyzed by spectrophotometric methods. The influence of the extraction time and the solvent on the yields of total extract (TEC), total phenolic compounds (TPhC), flavonoids (FC) and sesquiterpene lactones (SLC) has been studied. It was found that TEC and TPhC increased with increasing the solvent polarity in order CHCl3, MeOH and 80% MeOH. The extraction with MeOH gave maximum amount of flavonoids, while the SLC was found to be almost equal in all used solvents. UAE for 60 min and MAE for 60 s afforded higher or almost equal TEC, TPhC and FC than those obtained by CE for 24 h. The best extraction of sesquiterpene lactones was achieved with CHCl3 for 20 min of ultrasound treatment and 20 s of microwave irradiation.


Medicina ◽  
2007 ◽  
Vol 43 (6) ◽  
pp. 487 ◽  
Author(s):  
Agnė Kučinskaitė ◽  
Loretta Pobłocka-Olech ◽  
Mirosława Krauze-Baranowska ◽  
Małgorzata Sznitowska ◽  
Arūnas Savickas ◽  
...  

Salidroside and rosavins (rosavin, rosarin, and rosin), biologically active compounds of Rhodiola rosea L., were analyzed in dried roots and rhizomes of the plants cultivated in Lithuania and naturally growing in Altai geographical region in Russian Federation. The quantitative analysis of the aqueous-ethanolic extracts prepared by percolation was performed employing solid-phase extraction and thin-layer chromatography followed by densitometric determination. Similar amounts of salidroside were found in the extracts (1.35–1.62 mg/mL), irrespective of the origin of the crude plant; however, the differences in the profile of rosavins were substantial with higher content of total rosavins in the extracts obtained from the plant cultivated in Lithuania (7.4 vs. 4.2 mg/mL). It was demonstrated that extracts prepared with 70% (v/v) ethanol contained low concentration of salidroside (0.16–0.17 mg/mL), while the extraction of rosavins with 70% (v/v) ethanol was more efficient compared to 40% (v/v) ethanol.


Author(s):  
Marine Demetrashvili ◽  
◽  
Mariam Khomasuridze ◽  
Guram Tkemaladze ◽  
◽  
...  

Within the experiment, for preparation of the aromatized wines, enriched with biologically active compounds, were used the endemic medicinal plants and fruits. In the control and trial samples, by application of high performance liquid chromatography, were determined the content of total phenols, resveratrol, quercetin, myricetin and micronutrients. Compared to the corresponding control samples – sweet fortified wine, Saperavi and Hawthorn Red Aromatized Wine contents 21%, and Rkatsiteli, Kiwi and Elecampane White Aromatized Wine, 18.25% more total phenols. Based on conducted analyses, the trial samples contain twice more antioxidants, than they are typically identified in wines: Saperavi, Blackberry, Plum and Leonurus Red Aromatized Wine is characterized with the rich content of resveratrol, while, Rkatsiteli, buckthorn and balm with - quercetin and myricetin. These compounds are widely used in pharmaceutical industry. The enrichment of wines with these antioxidant is achieved by the usage of plants and herbs. Besides, the selected materials influenced on micronutrients, their content is also significantly increased. The obtained results confirm, that the selected materials and worked out recipes are effective for the production of beverages that have a positive effect on the human health.


2014 ◽  
Vol 1073-1076 ◽  
pp. 122-126
Author(s):  
Qiao Yang

In this study, a novel microwave-assisted extraction (MAE) method has been developed for the extraction and determination of the major biologically active compounds, crocin and its seven trans-cis analogues in a traditional Chinese medicine (TCM), gardenia fruits (Gardenia jasminoides Ellis) with analysis by reversed phase HPLC. Sudan I [1-phenylazo-2-naphthol] was selected as the internal standard. The results of quantitative determination of seven crocin analogues in six commercial gardenia fruits samples show that MAE–HPLC is a simple, rapid, low cost and reliable method for the determination of biologically active compounds, crocin analogues in TCM, and also a potential and powerful tool for quality assessment of gardenia fruits (Gardenia jasminoides Ellis).


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