scholarly journals Antifungal Activity of Essential Oil from Asteriscus graveolens against Postharvest Phytopathogenic Fungi in Apples

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Mohamed Znini ◽  
Gregory Cristofari ◽  
Lhou Majidi ◽  
Hamid Mazouz ◽  
Pierre Tomi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Direct Contact ◽  
Phytopathogenic Fungi ◽  
Penicillium Expansum ◽  
Rhizopus Stolonifer ◽  
Antifungal Effect ◽  
Aerial Parts ◽  
Alternaria Sp ◽  
Total Oil

The essential oils of the aerial parts of Asteriscus graveolens have been studied using GC and GC-MS. Twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. The aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% ±5.5) and 6-hydroxycyclonerolidol (8.8% ±1.2) as the major components. The antifungal effect of the essential oil from A. graveolens leaves was evaluated in vitro against three phytopathogenic fungi of apples ( Alternaria sp., Penicillium expansum, and Rhizopus stolonifer). The results suggest that this essential oil has fungicidal properties towards Alternaria sp. from direct contact assay at 0.2% (v/v) and to P. expansum from vapor assay tests at 80 μL.

scholarly journals Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Megil J. McNeil ◽  
Roy B. R. Porter ◽  
Lawrence A. D. Williams
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Oil Composition ◽  
Filter Paper Disc ◽  
Aerial Parts ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

scholarly journals Antifungal Activity of Aromatic Plants of the Lamiaceae Family in Bread

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1642
Author(s):  
Adriana Skendi ◽  
Dimitrios Ν. Katsantonis ◽  
Paschalina Chatzopoulou ◽  
Maria Irakli ◽  
Maria Papageorgiou
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
Phytopathogenic Fungi ◽  
Aromatic Plants ◽  
Inhibitory Effects ◽  
Antifungal Effect ◽  
Aerial Parts ◽  
Gas And Liquid Chromatography ◽  
Main Components

The antifungal effect of aromatic plants (oregano, thyme, and Satureja) in dry form and as essential oils was evaluated in vitro (in potato dextrose agar (PDA)) and in bread against two phytopathogenic fungi found in food (Aspergillusniger and Penicillium). Gas and liquid chromatography were used to analyze essential oils attained by hydrodistillation of the aerial parts of the aromatic plants and of the dried plant aqueous solutions that were autoclaved for 20 min at 121 °C before analysis. Carvacrol, α-pinene, p-cymene, and γ-terpinene were the main components of the essential oils, whereas carvacrol, rosmarinic and caffeic acids were the main components of the water extracts. In vitro antifungal test results showed that the addition of plants in dry form had great antifungal potential against both fungal strains studied. Penicillium was more sensitive to the presence of aromatic plants than Aspergillus. Among the three plant species tested, thyme was the most potent antifungal against both fungi. For the bread product, all three aromatic plants studied showed inhibitory effects against both fungi. Results presented here suggest that oregano, thyme and Satureja incorporated in a bread recipe possess antimicrobial properties and are a potential source of antimicrobial ingredients for the food industry.

scholarly journals In Vitro Antifungal Activity of Satureja horvatii ssp. macrophylla Against 3 Tomato Phytopathogenic Fungi

2021 ◽  
Vol 16 (7) ◽  
pp. 1934578X2110251
Author(s):  
Paraskevi Yfanti ◽  
George Patakioutas ◽  
Dimitra Douma ◽  
Marilena E. Lekka
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Aerial Part ◽  
Phytopathogenic Fungi ◽  
Major Constituent ◽  
Endemic Plant ◽  
Antifungal Effect ◽  
Endemic Plant Species ◽  
In Vitro Antifungal Activity

The essential oil from the aerial part of Satureja horvatii spp. macrophylla, an endemic plant species of northwest Greece has been analyzed using GC-MS. Thirty-nine compounds were identified, among which carvacrol was the major constituent (46.8%). The antifungal effect of the essential oil was investigated in vitro against 3 phytopathogenic fungi of tomato, using fumigant and contact assays. Fumigant assay was more effective than contact assay against all phytopathogens. The essential oil inhibited completely the mycelial growth of Fusarium oxysporum f. sp. lycopersici (123 µL L-1 air) and Alternaria alternatα (100 µL L-1 air), while it significantly reduced the growth of Botrytis cinerea (91%).

Antimicrobial Activity of Essential Oil and Major Constituents of Salvia chloroleuca

2008 ◽  
Vol 63 (5-6) ◽  
pp. 337-340 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Ali Sonboli ◽  
Samad Nejad Ebrahimi ◽  
Seid Hasan Hashemi
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Tehran Province ◽  
Main Components ◽  
Total Oil

The aerial parts of Salvia chloroleuca were collected at full flowering stage at Shahrestanak (Tehran province of Iran). The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. Thirty-four components were identified, representing 98.5% of the total oil. β-Pinene (10.6%), α-pinene (9.0%), β-caryophyllene (9.0%), 1,8-cineole (9.0%) and carvacrol (7.9%) were the main components. The in vitro antimicrobial activity of the essential oil of S. chloroleuca was studied against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger); the disc diffusion method and MIC values indicated that the oil exhibited moderate to high antimicrobial activity.

scholarly journals Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi

2006 ◽  
Vol 49 (6) ◽  
pp. 867-871 ◽  
Author(s):  
Terezinha de Jesus Faria ◽  
Rafael Sottero Ferreira ◽  
Lidiane Yassumoto ◽  
José Roberto Pinto de Souza ◽  
Noemia Kazue Ishikawa ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Steam Distillation ◽  
Ethanolic Extract ◽  
Phytopathogenic Fungi ◽  
Diffusion Method ◽  
Minimal Inhibitory Concentrations ◽  
Ocimum Gratissimum ◽  
Aerial Parts ◽  
Alternaria Sp

An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.

scholarly journals The Essential Oil of Artemisia scoparia from Tajikistan is Dominated by Phenyldiacetylenes

2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Farukh S. Sharopov ◽  
William N. Setzer
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Methyl Eugenol ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Aerial Parts ◽  
Artemisia Scoparia ◽  
Total Oil ◽  
Mcf 7

The essential oil from the aerial parts of Artemisia scoparia was obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. A total of 32 compounds were identified representing 98.0% of the total oil composition. A. scoparia oil was dominated by the diacetylenes 1-phenyl-2,4-pentadiyne (34.2%) and capillene (4.9%). Other major components were β-pinene (21.3%), methyl eugenol (5.5%), α-pinene (5.4%), myrcene (5.2%), limonene (5.0%), and ( E)-β-ocimene (3.8%). The oil was tested for in-vitro cytotoxic activity against MCF-7 cells, but was inactive.

scholarly journals Cytotoxicity, Antimicrobial Activity and Composition of Essential Oil from Tanacetum balsamita L. Subsp. Balsamita

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Samad Nejad Ebrahimi ◽  
Ali Sonboli ◽  
Farah Miraghasi ◽  
Shahla Ghiasi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Full Flowering ◽  
Main Components ◽  
Total Oil

The aerial parts of Tanacetum balsamita subsp. balsamita were collected at full flowering stage from Tabriz, Iran. The essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC and GC-MS. Twenty seven components were identified, representing 99.2% of the total oil. Carvone (51.0%), β-thujone (20.8%), 1,8-cineole (4.4%) and α-thujone (3.2%) were the main components. The in vitro antimicrobial activity of the essential oil of T. balsamita subsp. balsamita was studied against eight Gram-positive and Gram-negative bacteria ( Bacillus subtilis, B. pumulis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi ( Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial activity of the essential oil according to the disc diffusion method and MIC values indicated that the oil exhibited moderate to high antimicrobial activity. The in vitro toxicological study based on the MTT cytotoxicity assay on human fetal skin fibroblast (HFSF) and Monkey kidney (Vero) cell lines showed IC50 values of 2500 and 1250 μg/mL, respectively.

scholarly journals Antifungal activity of essential oils on mycelial growth of Fusarium oxysporum and Bortytis cinerea

2019 ◽  
pp. 544 ◽  
Author(s):  
Marina Palfi ◽  
Paško Konjevoda ◽  
Karolina Vrandečić
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
In Vitro Study ◽  
Phytopathogenic Fungi ◽  
Phytopathogenic Fungus ◽  
Antifungal Effect

In vitro study of the effect of different volumes of twelve essential oils on the mycelial growth of economically significant phytopathogenic fungi (Fusarium oxysporum and Botrytis cinerea) and it was compared to the effect of a fungicide. The antifungal activity of essential oils is decreased with the duration of incubation and it differs depending on the type of phytopathogenic fungus and the applied volume. The most effective antifungal effect on both tested fungi was in the essential oil of thyme, with lowest values of IC50 while the weakest effect was in essential oils of eucalyptus and lemon, with the highest values of IC50. Certain essential oils, when applied in certain volumes, had the same or even better effect on the inhibition of the growth of mycelium when compared to the tested fungicides.

Chemical Composition and in-vitro antimicrobial screening of leaf essential oil of Plectranthus gerardianus Benth., and isolation of the major constituent of oil

2020 ◽  
Vol 10 ◽  
Author(s):  
Navadha Bhatt ◽  
Navabha Joshi ◽  
Kapil Ghai ◽  
Om Prakash
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Major Constituent ◽  
In Vitro Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Medicinal Value ◽  
Leaf Essential Oil ◽  
Plant Families ◽  
Total Oil

Background: The Lamiaceae (Labiatae) is one of the most diverse and widespread plant families’ in terms of ethno medicine and its medicinal value is based on the volatile oils concentration. This family is important for flavour, fragrance and medicinal properties. Manyplants belonging to this family have indigenous value. Method: The essential oil of Plectranthus gerardianusBenth. (Lamiaceae), was analysed by GC and GC-MS analysis, while the major component was isolated and conformed by NMR spectroscopy. Result: The oil was found to be rich in oxygenated monoterpenes, which contribute around 62% of the total oil. The major components identified were fenchone (22.90%) and carvenone oxide (16.75%), besides other mono and sesquiterpenoids. The in-vitro antimicrobial activity of essential oil was tested against three gram negative bacteria viz. Pasteurellamultocida, Escherichia coli, and Salmonella enterica, two gram positive bacteria viz. Staphylococcus aureus and Bacillus subtilis and two fungi viz. Candida albicans and Aspergillusflavus. The antimicrobial activity of the oil was also compared to the antimicrobial activity of leaf essential oil of another Himalayan plant viz. Nepetacoerulescens. Conclusion: The oil showed in-vitro antimicrobial activity against all the microbial strains and can lessen the ever-growing demand of potentially hazardous antibiotics for treatment.

scholarly journals Minerals, Essential Oils, and Biological Properties of Melissa officinalis L.

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1066
Author(s):  
Fahima Abdellatif ◽  
Muhammad Akram ◽  
Samir Begaa ◽  
Mohammed Messaoudi ◽  
Adel Benarfa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Phytopathogenic Fungi ◽  
Melissa Officinalis ◽  
Flame Ionization ◽  
Analysis Technique ◽  
Total Oil

This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.

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