scholarly journals Antifungal Activity of Aromatic Plants of the Lamiaceae Family in Bread

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1642
Author(s):  
Adriana Skendi ◽  
Dimitrios Ν. Katsantonis ◽  
Paschalina Chatzopoulou ◽  
Maria Irakli ◽  
Maria Papageorgiou
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
Phytopathogenic Fungi ◽  
Aromatic Plants ◽  
Inhibitory Effects ◽  
Antifungal Effect ◽  
Aerial Parts ◽  
Gas And Liquid Chromatography ◽  
Main Components

The antifungal effect of aromatic plants (oregano, thyme, and Satureja) in dry form and as essential oils was evaluated in vitro (in potato dextrose agar (PDA)) and in bread against two phytopathogenic fungi found in food (Aspergillusniger and Penicillium). Gas and liquid chromatography were used to analyze essential oils attained by hydrodistillation of the aerial parts of the aromatic plants and of the dried plant aqueous solutions that were autoclaved for 20 min at 121 °C before analysis. Carvacrol, α-pinene, p-cymene, and γ-terpinene were the main components of the essential oils, whereas carvacrol, rosmarinic and caffeic acids were the main components of the water extracts. In vitro antifungal test results showed that the addition of plants in dry form had great antifungal potential against both fungal strains studied. Penicillium was more sensitive to the presence of aromatic plants than Aspergillus. Among the three plant species tested, thyme was the most potent antifungal against both fungi. For the bread product, all three aromatic plants studied showed inhibitory effects against both fungi. Results presented here suggest that oregano, thyme and Satureja incorporated in a bread recipe possess antimicrobial properties and are a potential source of antimicrobial ingredients for the food industry.

Chemical Composition and in vitro Antimicrobial Activity of the Essential Oils of Two Helichrysum Species from Tanzania

2004 ◽  
Vol 59 (5-6) ◽  
pp. 368-372 ◽  
Author(s):  
Christos Bougatsos ◽  
Olipa Ngassapa ◽  
Deborah K. B. Runyoro ◽  
Ioanna B. Chinou
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Fungi ◽  
Caryophyllene Oxide ◽  
In Vitro Antimicrobial Activity ◽  
Aerial Parts ◽  
Main Components

The chemical composition of the essential oils obtained from the aerial parts of Helichrysum cymosum and H. fulgidum, from Tanzania, were analyzed by GC and GC/MS. A total of sixty-five compounds, representing 92.4% and 88.2% of the two oils, respectively, were identified. trans-Caryophyllene, caryophyllene oxide, β-pinene, p-cymene, spathulenol and β- bourbonene were found to be the main components. Furthermore, the oils were tested against six gram (±) bacteria and three pathogenic fungi. It was found that the oil of H. fulgidum exhibited significant antimicrobial activity, while the oil of H. cymosum was not active at all.

scholarly journals Antimicrobial properties of phytohormone (gibberellins) against phytopathogens and clinical pathogens

2021 ◽  
Vol 3 (10) ◽  
Author(s):  
Paoirse Toner ◽  
David Nelson ◽  
Juluri R. Rao ◽  
Madeleine Ennis ◽  
John E. Moore ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Microbial Pathogens ◽  
Inhibitory Effects ◽  
Plant Host ◽  
Therapeutic Value ◽  
Wide Range

The in vitro antimicrobial potential of physiologically active diterpenoid plant-derived gibberellins (gibberellic acids; GAs) was tested on microbial pathogens of significance to plant and human health. The racemic enantiomer GA3 produced varying inhibitory effects against a wide range of plant host disease causal agents (phytopathogens) comprising fungi, oomycetes and bacteria. The results showed that GA3 effected either strong growth arrest of phytopathogenic fungi or holistic biocidal effects on oomycete and phytopathogenic fungi at higher concentration (>10–50 mM) and increased hyphal extension growth when the concentration of GA3 was lowered (<10−0.1 mM). When human clinical pathogenic bacteria cohorts were challenged with gibberellin enantiomers, viz GA1, GA4, GA5, GA7, GA9 and GA13 (50 mM), employing Kirby–Bauer disc bioassay methods for assessment of their efficacies, no inhibitory effect was seen with gibberellin enantiomers, viz GA1, GA3, GA5 and GA13, while GA4 inhibited all human clinical bacterial organisms examined, with GA7 and GA9 showing limited activity. The antibiotic effects of enantiomeric diterpenoid phytohormones evinced in our preliminary study raise prospects for further studies to fully examine their potential therapeutic value for human healthcare and their compliance with cytotoxicity and other ethical considerations in the future.

scholarly journals ESSENTIAL OIL COMPOSITION OF TWO SPECIES OF SCUTELLARIA AERIAL PARTS

2021 ◽  
pp. 139-144
Author(s):  
Abdurashid Musakhonovich Karimov ◽  
Khairulla Mamadievich Bobakulov ◽  
Yulia Vladimirovna Ostroushko ◽  
Erkin Khozhiakbarovich Botirov ◽  
Azimjon Akparalievich Mamadrakhimov ◽  
...  
Keyword(s):  
Essential Oils ◽  
Folk Medicine ◽  
Antimicrobial Properties ◽  
Essential Oil Composition ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Aerial Parts ◽  
Moderate Effect ◽  
Main Components ◽  
First Time

The chemical composition of essential oils obtained by hydrodistillation method from two plants of the genus Scutellaria, grown in Uzbekistan and used in folk medicine were comparatively investigated by GC/MS and FID. Overall individually thirty three constituents were identified in both of aerial parts of S. adenostegia and S. comosa essential oils, representing 94.4 and 97.0% of the total, respectively. The main components were determined as acetophenone (24.2%), eugenol (12.3%), caryophyllene oxide (8.9%), and β-caryophyllene (7.0%) in the oil of S. adenostegia. β-Caryophyllene (12.5%), phytol (11.4%), linalool (11.1%), acetophenone (10.4%), caryophyllene oxide (6.6%),1-hexanol (5.3%), and (E)-2-hexenal (5.1%) were found as major components in the S. comosa oil. The composition of the oils of S. adenostegia and S. comosa was being reported for the first time. The essential oils of S. adenostegia and S. comosa showed significant antimicrobial properties against Bacillus subtilis, moderate effect against Salmonella enterica and Escherichia coli.

scholarly journals Antifungal Activity of Essential Oil from Asteriscus graveolens against Postharvest Phytopathogenic Fungi in Apples

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Mohamed Znini ◽  
Gregory Cristofari ◽  
Lhou Majidi ◽  
Hamid Mazouz ◽  
Pierre Tomi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Direct Contact ◽  
Phytopathogenic Fungi ◽  
Penicillium Expansum ◽  
Rhizopus Stolonifer ◽  
Antifungal Effect ◽  
Aerial Parts ◽  
Alternaria Sp ◽  
Total Oil

The essential oils of the aerial parts of Asteriscus graveolens have been studied using GC and GC-MS. Twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. The aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% ±5.5) and 6-hydroxycyclonerolidol (8.8% ±1.2) as the major components. The antifungal effect of the essential oil from A. graveolens leaves was evaluated in vitro against three phytopathogenic fungi of apples ( Alternaria sp., Penicillium expansum, and Rhizopus stolonifer). The results suggest that this essential oil has fungicidal properties towards Alternaria sp. from direct contact assay at 0.2% (v/v) and to P. expansum from vapor assay tests at 80 μL.

Antifungal mechanisms of lavender essential oil in the inhibition of rot disease caused by Monilinia fructicola in postharvest flat peaches

2021 ◽  
Author(s):  
Xiaolin Xiong ◽  
Lingling Zhang ◽  
Xingyan Li ◽  
Qingzhi Zeng ◽  
Rongrong Deng ◽  
...  
Keyword(s):  
Essential Oil ◽  
Membrane Damage ◽  
Phytopathogenic Fungi ◽  
Monilinia Fructicola ◽  
Inhibitory Effects ◽  
Rot Disease ◽  
Main Components ◽  
Fungistatic Effect

As a natural antimicrobial agent, lavender essential oil (LEO) is generally recognized to be safe and effective in the inhibition of phytopathogenic fungi. Direct contact and fumigation (in vivo and in vitro) were used to study the fungistatic effect of LEO on Monilinia fructicola. Additionally, the effect on the ultrastructure of cells and degree of destruction of the cell membrane of M. fructicola were revealed. In addition, the effects of LEO on the expression levels of particular apoptosis-related genes in M. fructicola cells were detected and GC-MS was used to analyse the main components of LEO. LEO had a good inhibitory efficacy against M. fructicola in flat peaches, with almost complete growth inhibition with 800 μL / L. These effects were associated with leakage of cytoplasm contents, hyphal distortion and spore disruption. Moreover, the expression of apoptosis RTG1 and RLM1 genes increased on LEO treatment. These results demonstrate that LEO can inhibit M. fructicola by inducing cytoplasmic membrane damage and cell apoptosis of fungi and that the major ingredients of LEO are monoterpenes and sesquiterpenes which are presumed to contribute to the inhibitory effects.

scholarly journals Antifungal activity of essential oils on mycelial growth of Fusarium oxysporum and Bortytis cinerea

2019 ◽  
pp. 544 ◽  
Author(s):  
Marina Palfi ◽  
Paško Konjevoda ◽  
Karolina Vrandečić
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
In Vitro Study ◽  
Phytopathogenic Fungi ◽  
Phytopathogenic Fungus ◽  
Antifungal Effect

In vitro study of the effect of different volumes of twelve essential oils on the mycelial growth of economically significant phytopathogenic fungi (Fusarium oxysporum and Botrytis cinerea) and it was compared to the effect of a fungicide. The antifungal activity of essential oils is decreased with the duration of incubation and it differs depending on the type of phytopathogenic fungus and the applied volume. The most effective antifungal effect on both tested fungi was in the essential oil of thyme, with lowest values of IC50 while the weakest effect was in essential oils of eucalyptus and lemon, with the highest values of IC50. Certain essential oils, when applied in certain volumes, had the same or even better effect on the inhibition of the growth of mycelium when compared to the tested fungicides.

scholarly journals Foeniculum vulgare Essential Oils: Chemical Composition, Antioxidant and Antimicrobial Activities

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Maria Graça Miguel ◽  
Cláudia Cruz ◽  
Leonor Faleiro ◽  
Mariana T. F. Simões ◽  
Ana Cristina Figueiredo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Point Of View ◽  
Diffusion Method ◽  
Foeniculum Vulgare ◽  
Aerial Parts ◽  
Scavenging Capacity ◽  
Dpph Method ◽  
Main Components

The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), α-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity >50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

scholarly journals Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2124
Author(s):  
Giulia Vanti ◽  
Ekaterina-Michaela Tomou ◽  
Dejan Stojković ◽  
Ana Ćirić ◽  
Anna Rita Bilia ◽  
...  
Keyword(s):  
Essential Oils ◽  
Propylene Glycol ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Spoilage Microorganisms ◽  
Satureja Thymbra ◽  
Origanum Onites

Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.

scholarly journals Assessment of the efficiency of essential oils in the preservation of postharvest papaya in an antimicrobial packaging system

2012 ◽  
Vol 15 (4) ◽  
pp. 333-342 ◽  
Author(s):  
Paula Judith Perez Espitia ◽  
Nilda de Fátima Ferreira Soares ◽  
Laura Costa Moreira Botti ◽  
Nathália Ramos de Melo ◽  
Olinto Liparini Pereira ◽  
...  
Keyword(s):  
Essential Oils ◽  
Physicochemical Parameters ◽  
Phytopathogenic Fungi ◽  
Soluble Solids ◽  
Postharvest Diseases ◽  
Packaging System ◽  
Antimicrobial Packaging ◽  
Post Harvest ◽  
Lemon Grass

Rot and damage caused by post-harvest phytopathogenic fungi affect fruit quality. Essential oils (EO) are considered as an alternative to fungicides. Postharvest diseases of fruits may also be controlled by the bagging approach and the use of antimicrobial packaging. Based on the beneficial properties of EO and the concepts of bagging and antimicrobial packaging, this study aimed to develop sachets containing EO to be used as part of an antimicrobial packaging system. The activities of oregano, cinnamon and lemon grass EO were evaluated testing the sachets in vitro against the phytopathogenic fungi Alternaria alternata, Fusarium semitectum, Lasiodiplodia theobromae and Rhizopus stolonifer. The effects of the sachets on the microbiological and physicochemical parameters of post-harvest papaya were also evaluated. Both pure and sachet-incorporated EO showed antifungal activity in vitro against all tested fungi. For papaya, sachets containing cinnamon, oregano and lemon grass showed a significant reduction in the growth of mesophilic aerobic bacteria, yeasts and mould, with the cinnamon sachet causing the greatest reduction in microorganisms at the end of the storage time. Physicochemical parameters of papaya, such as weight loss, colour, firmness, total soluble solids/titratable acidity ratio and pH were not significantly altered by the presence of EO sachets, thus not affecting the natural ripening process of the papaya.

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