scholarly journals HPLC Determination of Majdine in Vinca herbacea

2011 ◽  
Vol 6 (12) ◽  
pp. 1934578X1100601
Author(s):  
Natia Gagua ◽  
Beatrice Baghdikian ◽  
Fathi Mabrouki ◽  
Riad Elias ◽  
Valentina Vachnadze ◽  
...  
Keyword(s):  
Quality Control ◽  
Chromatographic Separation ◽  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Solvent System ◽  
Hplc Method ◽  
Potassium Phosphate Buffer ◽  
Good Linear ◽  
Linear Behavior

A reliable HPLC method coupled with DAD detection was developed and validated for determination of majdine in Vinca herbacea. The chromatographic separation was carried out on a Symmetry C18 column (250 mm x 4.6 mm, 5 μm, Waters) with an isocratic solvent system of 25 mM potassium phosphate buffer (pH=3.0)-acetonitrile. UV detection was performed at 225 nm. Good linear behavior over the investigated concentration range was observed with the value of r2 > 0.9978. The method was reproducible with intra- and inter-day variations of less than 4.38%. The proposed method was linear, accurate, precise and specific. The validated method was successfully applied to quantify majdine in various parts of V. herbacea, which was collected during the flowering months of April and May. The results indicated that the developed HPLC method could be used for the quality control of V. herbacea and for the standardization of its extracts in majdine.

scholarly journals Simultaneous HPLC Determination of Three Bioactive Alkaloids in the Asian Medicinal Plant Stephania rotunda

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Sothavireak Bory ◽  
Sok-Siya Bun ◽  
Béatrice Baghdikian ◽  
Fathi Mabrouki ◽  
Sun Kaing Cheng ◽  
...  
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Solvent System ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Linear Behavior ◽  
Photodiode Array ◽  
Array Detection ◽  
The Mean

A reliable high-performance liquid chromatography (HPLC) method coupled with photodiode array detection has been developed and validated for the determination of three major alkaloids: cepharanthine, tetrahydropalmatine and xylopinine in Stephania rotunda Lour. (Menispermaceae) collected in Cambodia. The chromatographic separation was carried out on a Symmetry C8 column (250 mm x 4.6 mm, 5 μm, Waters), with an isocratic solvent system of 25 mM potassium phosphate buffer (pH 3.5) – acetonitrile. UV detection was performed at 282 nm. Good linear behavior over the investigated concentration ranges was observed with values of r2>0.9964 for all the analytes. The method was reproducible with intra- and inter-day variations of less than 3.91%. The mean recoveries of the analytes ranged from 95.7 to 104.6%. The proposed method was linear, accurate, precise and specific. The validated method was successfully applied to quantify the three alkaloids in various parts of Stephania rotunda and in tubers collected from different Cambodian regions. The results indicated that the developed HPLC method could be used for the quality control of S. rotunda.

HPLC method for measurement of purine nucleotide degradation products in cerebrospinal fluid

1996 ◽  
Vol 42 (5) ◽  
pp. 756-760 ◽  
Author(s):  
L Kuracka ◽  
T Kalnovicová ◽  
B Líska ◽  
P Turcáni
Keyword(s):  
Cerebrospinal Fluid ◽  
Uric Acid ◽  
Neurological Disorders ◽  
Limit Of Detection ◽  
Degradation Products ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Potassium Phosphate Buffer ◽  
Nucleotide Degradation

Abstract We describe a convenient method for the separation and quantification of xanthine, hypoxanthine, and uric acid in 20 microL of cerebrospinal fluid (CSF) with use of HPLC and ultraviolet detection. The analysis is performed on a Sepharon SGX C18 column and the elution system consists of potassium phosphate buffer, pH 5.1, with 20 mL/L methanol. The lower limit of detection was 4 pmol for hypoxanthine and xanthine and 6 pmol for uric acid. Analytical recoveries of purine metabolites ranged from 98.6% to 102.9%. The intra- and interassay CVs were <3%. The applicability of the method is illustrated with the determination of micromolar concentrations of xanthine, hypoxanthine, and uric acid in CSF samples obtained from 113 patients with various neurological disorders.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

SIMULTANEOUS DETERMINATION OF PARACETAMOL, ACECLOFENAC AND TRAMADOL HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (01) ◽  
pp. 35-40
Author(s):  
Rajesh Sharma ◽  
◽  
Mukesh C. Sharma ◽  
Gaurav Vijaywargiya
Keyword(s):  
Flow Rate ◽  
Chromatographic Separation ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Dosage Form ◽  
Hplc Method ◽  
Tramadol Hydrochloride ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc

Chromatographic separation of paracetamol, aceclofenac and tramadol hydrochloride was performed on a Chromatopak C-18 column (25 cm x 4.6mm i.d. x 5µm) as stationary phase with a mobile phase composed of phosphate buffer pH 7.0: acetonitrile (65:35 V/V), pH 7.0 (adjusted with triethylamine) at flow rate of 1mL/min. Detection was carried out at 265 nm. The retention times of paracetamol, aceclofenac and Tramadol hydrochloride were found to be 2.7, 4.5 and 6.0 min, respectively. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The method was found to be accurate, precise, specific, robust, and linear for the determination of paracetamol, aceclofenac and tramadol hydrochloride in pharmaceutical dosage form.

scholarly journals Development and Validation of an HPLC Method for Mefloquine Hydrochloride Determination in Tablet Dosage Form

2011 ◽  
Vol 94 (4) ◽  
pp. 1089-1093 ◽  
Author(s):  
Fernando Henrique Andrade Nogueira ◽  
Letícia De Paula Lana Goulart ◽  
Isabela Da Costa César ◽  
Lígia Maria Moreira De Campos ◽  
Gérson Antônios Pianetti
Keyword(s):  
Mobile Phase ◽  
Degradation Products ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Stability Studies ◽  
Potassium Phosphate Buffer ◽  
Photolytic Degradation ◽  
Development And Validation ◽  
Tablet Dosage

Abstract A simple HPLC method for determination of mefloquine hydrochloride in tablets was developed and validated. The separation was carried out on an Xterra RP18 (250 × 4.6 mm id, 5 µm particle size) analytical column. The mobile phase was 0.05 M monobasic potassium phosphate buffer (pH 3.5)–methanol (40 + 60, v/v). The flow rate and wavelength were set to 1 mL/min and 283 nm, respectively. The method was specifc for mefloquine hydrochloride in the presence of hydrolytic, oxidative, and photolytic degradation products. It was also linear, precise, accurate, and robust, being suitable for routine QC analyses and stability studies. The developed HPLC method was compared to a previously described spectrophotometric method.

scholarly journals Determination of Histidine and Related Compounds in Rumen Fluid by Liquid Chromatography

2000 ◽  
Vol 83 (1) ◽  
pp. 8-15 ◽  
Author(s):  
Shaila Wadud ◽  
Ryoji Onodera ◽  
Mamun M Or-Rashid ◽  
Mohammad R Amin
Keyword(s):  
Phosphate Buffer ◽  
Rumen Fluid ◽  
Direct Injection ◽  
Potassium Phosphate ◽  
Potassium Phosphate Buffer ◽  
Absorbance Detection ◽  
Before And After ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract A liquid chromatographic procedure was developed for quantitative determination of histidine (His), histidinol (HDL), histamine (HTM), urocanic acid (URA), imidazolepyruvic acid (ImPA), imidazoleacetic acid (ImAA), and imidazolelactic acid (ImLA) in rumen fluid. The method is based on direct injection analysis by UV absorbance detection at 220 nm. The separation was performed under 2 different chromatographic conditions on a LiChrospher 100 NH2 column. In the first chromatographic system, the mobile phase used for isocratic elution was 67 mM potassium phosphate buffer (monobasic and dibasic) pH 6.45–90% acetonitrile in water (21 + 79); in the second system, an acetonitrile gradient in 63 mM potassium phosphate buffer (monobasic) pH 3.0, obtained by addition of 60 mM phosphoric acid, was used. Analyses of both systems were completed within 32 and 25 min, respectively. The limits of detection of these compounds were (μM): His, 2.8; HDL, 3.7; HTM, 4.0; URA, 0.75; ImPA, 4.7; ImAA, 1.2; and ImLA, 1.3. Recovery of these compounds added to rumen fluid was 97.4–103.0% within a 1-day study and 95.4–99.0% on different day studies. Detectable levels of His were found in the deproteinized rumen fluid of goats, with average concentrations of 16.10, 10.43, 11.14, and 13.62 μM in the rumen fluid collected before the morning feeding and 2, 4, and 6 h after feeding, respectively. HDL, HTM, URA, ImPA, ImAA, and ImLA were not detected in the rumen fluid before and after feeding. Trp, Phe, and Tyr were also identified in the rumen fluid, with average concentrations of 8.25, 29.04, and 12.6 μM, respectively, before the morning feeding.

Detection and Evaluation of process related impurities in Obeticholic Acid Drug material using HPLC Method

2021 ◽  
pp. 5618-5624
Author(s):  
Kishore Gaddam ◽  
Srinivas Kumbam ◽  
Trivikram Reddy Gundala ◽  
Surendranath Reddy Reddiwary ◽  
Gangi Reddy Nallagondu Chinna
Keyword(s):  
Orthophosphoric Acid ◽  
Detection Method ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Biliary Cirrhosis ◽  
Potassium Phosphate Buffer ◽  
Obeticholic Acid ◽  
Related Substances ◽  
Refractive Index Detection

Obeticholic acid (OBE) is being used to treat primary biliary cirrhosis and cholangitis. An HPLC with refractive index detection method for eight process related substances of OBE was developed and verified for use in quality assurance laboratories for regular analysis. The separation and analysis were performed on YMC Triart C18 (3.0 µm particle size, 250 mm × 4.6 mm) column. Mobile phase employed consisted of 0.01N potassium phosphate buffer (3.0 ± 0.05 pH, set with 0.1% orthophosphoric acid) and acetonitrile at 45:55 (v/v) ratio. The method established for determination of eight impurities in OBE was validated and verified in keeping with International Council for Harmonisation guidelines. This method can be used for routine analysis of eight impurities in OBE bulk samples.

Simultaneous determination of neopterin and creatinine in serum with solid-phase extraction and on-line elution liquid chromatography.

1987 ◽  
Vol 33 (11) ◽  
pp. 2028-2033 ◽  
Author(s):  
E R Werner ◽  
D Fuchs ◽  
A Hausen ◽  
G Reibnegger ◽  
H Wachter
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Phosphate Buffer ◽  
Serum Proteins ◽  
Solid Phase ◽  
Potassium Phosphate ◽  
Sample Volume ◽  
Potassium Phosphate Buffer ◽  
Standard Curve

Abstract This is a method for the simultaneous determination of neopterin, a product of interferon-gamma-activated macrophages, and creatinine in serum. Acidified, but not deproteinized serum is applied to a 4-propylbenzene sulfonic acid-modified silica sorbent cartridge, which quantitatively retains the analytes but not the serum proteins. The retained analytes are then eluted from the cartridge directly onto the liquid-chromatography column. Elution from the cartridge is facilitated by a pulse of 0.4 mol/L, pH 6.8 potassium phosphate buffer. On isocratic elution from an octadecylsilica column with potassium phosphate buffer (15 mmol/L, pH 6.0), neopterin is detected by its native fluorescence, creatinine by ultraviolet absorption. Detection limits are 0.5 nmol/L for neopterin, 1 mumol/L for creatinine at a sample volume of 100 microL. The standard curve is linear over the range of concentrations encountered in sera. For both neopterin and creatinine in serum, concentrations so measured agree well with results by established methods.

A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Imad Tarek Hanoon ◽  
Abed Mohammed Daheir AL-Joubory 2 ◽  
Marwa Mohamed Saied 3
Keyword(s):  
Mobile Phase ◽  
Chemical Structure ◽  
Quantitative Estimation ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Form ◽  
Rp Hplc ◽  
Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

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