scholarly journals Characterization of Polyphenolic Compounds in Unripe Chinotto (Citrus myrtifolia) Fruit by HPLC/PDA/ESI/MS-MS

2011 ◽  
Vol 6 (12) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Monica Scordino ◽  
Leonardo Sabatino ◽  
Adalgisa Belligno ◽  
Giacomo Gagliano
Keyword(s):  
Soft Drink ◽  
Polyphenolic Compounds ◽  
Relative Distribution ◽  
Lower Content ◽  
Esi Ms ◽  
Negative Mode ◽  
Polymethoxylated Flavones ◽  
Unripe Fruits

The flavonoid and furocoumarin composition was investigated of peel and pulp tissues of unripe fruits of Citrus myrtifolia Rafinesque, an ingredient of the popular soft drink “chinotto”. Compound separation and identification was made using an HPLC-PDA detector coupled to ESI/MS/MS in positive and negative mode. Eighteen compounds (3-hydroxy-3-methylglutaryl-, C- and O-glycosyl flavonoids, furocoumarins and polymethoxylated flavones) were identified and quantified. Data indicated that the overall amount of flavonoids and furocoumarins in peel was higher than in the pulp, even though their relative distribution did not significantly change, apart from a different distribution of flavones and a lower content of naringin in the peel.

scholarly journals Rapid Characterization of Triterpene Saponins from Zornia brasiliensis by HPLC-ESI-MS/MS

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2519 ◽  
Author(s):  
Yuri Mangueira Nascimento ◽  
Lucas Silva Abreu ◽  
Ramon Leal Lima ◽  
Vicente Carlos O. Costa ◽  
José Iranildo Miranda de Melo ◽  
...  
Keyword(s):  
High Performance ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry ◽  
Mass Determination ◽  
Triterpene Saponins ◽  
Esi Ms ◽  
Negative Mode ◽  
Array Detection ◽  
Rapid Characterization

Zornia brasiliensis Vogel (Leguminosae) is a species popularly known in Brazil as “urinária”, “urinana”, and “carrapicho”, it is popularly used as a diuretic and in the treatment of venereal diseases. A specific methodology to obtain a saponin-enriched fraction and high-performance liquid chromatography coupled with diode array detection, ion trap mass spectrometry, and TOF-MS (HPLC-DAD-ESI-MS/MS) was applied for the analysis of triterpene saponins. The MS and MS/MS experiments were carried out by ionization in negative mode. Molecular mass and fragmentation data were used to support the structural characterization of the saponins. Based on retention times, high-resolution mass determination and fragmentation, 35 oleanane-triterpene saponins were tentatively identified in Z. brasiliensis.

HPLC-ESI-MS Characterization of Certain Polyphenolic Compounds of Carica papaya L. Fruit Extracts and Evaluation of Their Potential Against Murine Schistosomiasis mansoni

Drug Research ◽  
2018 ◽  
Vol 68 (09) ◽  
pp. 521-528 ◽  
Author(s):  
Ezzat Abdel-Lateef ◽  
Ibrahim Rabia ◽  
Mortada El-Sayed ◽  
El-Sayed Abdel-Hameed
Keyword(s):  
Worm Burden ◽  
Carica Papaya ◽  
Methanolic Extract ◽  
Polyphenolic Compounds ◽  
Infected Group ◽  
Esi Ms ◽  
Methanolic Extracts ◽  
Ms Analysis

AbstractThe in vivo antischistosomal activities of Carica papaya L. extracts were evaluated and the characterization of the active secondary metabolites of the defatted methanolic extract was performed using HPLC-ESI-MS. The plant fruit powders were extracted with 85% methanol and fractionated using organic solvents. The in vivo antischistosomal effects of the methanolic extracts and its fractions, as well as the assessment of the relationship between the antischistosomal activity of these plant extracts and oxidative stress, was determined. In addition, the defatted methanolic extract was characterized by HPLC-ESI-MS analysis. The number of worms, ova, and the Oogram pattern displayed typical Schistosoma mansoni pathology 8 weeks after infection in mice. Treatment of the infected group with the defatted methanolic extracts significantly decreased worm burden, immature ova and mature ova, while increasing the percentage of dead ova in vivo. The butanol fraction was the most effective fraction reducing worm burden by 77%, ova count in the intestine by 76% and in the liver by 80%, and significantly decreased immature and mature ova (P<0.001) compared to the infected group. Additionally, the defatted methanolic extracts improved the reduced glutathione and malondialdehyde levels in hepatic tissues in the treated groups compared to the infected group. The HPLC-ESI-MS analysis of the Carica papaya defatted methanolic extract revealed the presence of several polyphenolic compounds. Carica papaya fruit extracts are rich with phenolic acids and flavonoids and show a significant effect against S. mansoni infections which may be used alternative to PZQ as anti-schistosomal drug against schistosomiasis.

Characterization of thirty-nine polymethoxylated flavonoids (PMFs) in the branches of Murraya paniculata by HPLC-DAD-ESI-MS/MS

2014 ◽  
Vol 11 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Jia-Yu ZHANG ◽  
Jian-Qiu LU ◽  
Xiao-Yan GAO ◽  
Qian ZHANG ◽  
Ning LI ◽  
...  
Keyword(s):  
Esi Ms ◽  
Murraya Paniculata

Biological activities of two polypore macrofungi (Basidiomycota) and characterization of their compounds using HPLC–DAD and LC–ESI–MS/MS

2021 ◽  
Author(s):  
Samaneh Chaharmiri Dokhaharani ◽  
Masoomeh Ghobad-Nejhad ◽  
Hamid Moghimi ◽  
Abbas Farazmand ◽  
Hossein Rahmani
Keyword(s):  
Biological Activities ◽  
Esi Ms

scholarly journals Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 799
Author(s):  
Ana G. Abril ◽  
Mónica Carrera ◽  
Karola Böhme ◽  
Jorge Barros-Velázquez ◽  
Benito Cañas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Products ◽  
Phylogenomic Analysis ◽  
Esi Ms ◽  
Complement Inhibitors ◽  
Staphylococcus Spp ◽  
The Relationship ◽  
Phage Proteins ◽  
Identification And Characterization

The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.

Structural characterization of native and oxidized procyanidins (condensed tannins) from coffee pulp (Coffea arabica) using phloroglucinolysis and thioglycolysis-HPLC-ESI-MS

2021 ◽  
Vol 340 ◽  
pp. 127830
Author(s):  
Jorge E. Wong-Paz ◽  
Sylvain Guyot ◽  
Pedro Aguilar-Zárate ◽  
Diana B. Muñiz-Márquez ◽  
Juan C. Contreras-Esquivel ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Coffea Arabica ◽  
Condensed Tannins ◽  
Coffee Pulp ◽  
Esi Ms
Sign in / Sign up

Export Citation Format

Share Document