scholarly journals Antioxidant, Antimicrobial, and Cytotoxic Properties of Aniba parviflora Essential Oils from the Amazon

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Joyce Kelly R. da Silva ◽  
José Guilherme S. Maia ◽  
Noura S. Dosoky ◽  
William N. Setzer
Keyword(s):  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Dpph Assay ◽  
Breast Tumor Cells ◽  
Antibacterial Screening ◽  
High Concentrations ◽  
Mcf 7

Essential oils (EOs) from leaves and branches of Aniba parviflora (Meisn.) Mez were obtained by hydrodistillation and their chemical compositions determined by gas chromatography-mass spectrometry. Oxygenated sesquiterpenoids were the most representative compound class in both EOs ( ≅ 40%). However, the leaf EO was rich in β-phellandrene (15.1%), linalool (14.1%), and γ-eudesmol (12.9%). The EO from the branches showed high concentrations of γ-eudesmol (16.8%), β-caryophyllene (15.7%), linalool (12.4%), β-phellandrene (6.7%), and bicyclogermacrene (6.0%). The EOs were screened for radical-scavenging activity using the DPPH assay. In the DPPH assay, the EOs showed an antioxidant activity with TEAC values of 296.8 ± 20.2 and 357.3 ± 43.8 mg.TE.mL−1, about one-third that of the Trolox standard. The EOs were subjected to antibacterial screening and displayed promising activity against Escherichia coli (MIC < 19.5 μg.mL−1). In addition, the EOs were tested for cytotoxic activity against MCF-7 breast tumor cells and the leaf EO (IC50 = 67.9 ± 3.0 μg.mL−1) was more active than that from the branches (IC50 = 102.2 ± 3.0 μg.mL−1).

scholarly journals Chemical Profile and in vitro Biological Activities of Essential Oils of Nectandra puberula and N. cuspidata from the Amazon

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Joyce Kelly R. da Silva ◽  
Eloísa Helena A. Andrade ◽  
Rosa Helena V. Mourão ◽  
José Guilherme S. Maia ◽  
Noura S. Dosoky ◽  
...  
Keyword(s):  
Essential Oils ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Chemical Profile ◽  
Breast Tumor Cells ◽  
Antibacterial Screening ◽  
Germacrene D ◽  
Mcf 7

Essential oils (EO) from leaves and branches of Nectandra puberula Schott (Nees) and from leaves of N. cuspidata Nees & Mart. were obtained by hydrodistillation and their chemical compositions determined by gas chromatography - mass spectrometry (GC-MS). The main compounds identified from N. puberula EO were apiole (22.2%), β-caryophyllene (15.1%), β-pinene (13.3%), germacrene D (8.3%), pogostol (6.6%) and bicyclogermacrene (6.4%) in the leaves; and apiole (28.1%), pogostol (19.8%) and guaiol (11.2%) in the branches. The EO of N. cuspidata leaves showed β-caryophyllene (26.9%), bicyclogermacrene (16.0%) and spathulenol (5.2%) as the main compounds. The EOs were subjected to antibacterial screening and displayed promising activity against Escherichia coli (MIC = 19.5 μg.mL−1). In addition, the EOs were tested for cytotoxic activity against MCF-7 breast tumor cells and the IC50 values were 64.5 ± 1.6 and 117.1 ± 11.9 μg.mL−1 for the leaf EOs of N. puberula and N. cuspidata, respectively.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Lavandula Stoechas L. Ssp. Stoechas Growing Wild in Turkey

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Hasan Kırmızıbekmez ◽  
Betül Demirci ◽  
Erdem Yeşilada ◽  
K. Hüsnü Can Başer ◽  
Fatih Demirci
Keyword(s):  
Essential Oils ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Compositions ◽  
Pathogenic Microorganisms ◽  
Dpph Assay ◽  
Lavandula Stoechas ◽  
Leaf Oil ◽  
Main Components

The chemical compositions of the essential oils obtained by hydrodistillation from the dried leaves and flowers of Lavandula stoechas L. ssp. stoechas were separately identified by GC-FID and GCMS analyses. The main components were α-fenchone (41.9±1.2%), 1,8-cineole (15.6±0.8%), camphor (12.1±0.5%), and viridiflorol (4.1±0.4%) in the leaves; and α-fenchone (39.2±0.9%), myrtenyl acetate (9.5±0.4%), α-pinene (6.1±0.09%), camphor (5.9±0.05%) and 1,8-cineole (3.8±0.1%) in the flowers. Overall, 55 and 66 constituents were identified in the leaf and flower essential oils representing more than 90% and 94% of the total, respectively. In addition, the essential oils were evaluated for their antibacterial and anticandidal activities by broth microdilution. The flower essential oil was found to be relatively more active than the leaf oil towards the tested pathogenic microorganisms. Methicillin-resistant Staphylococcus aureus was more susceptible to the flower oil (MIC= 31.2 μg/mL). The oils, evaluated for their free radical scavenging activity using a TLC-DPPH assay, were inactive at a concentration of 2 mg/mL.

Composition of essential oil and antioxidant activity of Khat (Catha edulis Forsk), Ethiopia

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Bahir Dar ◽  
Dpph Radical ◽  
Catha Edulis

In this study, we determined the chemical composition and antioxidant activities of the essential oils from two different varieties of khat (Catha edulis Forsk) cultivated in Ethiopia. The essential oils were extracted by hydrodistillation using the Clevenger type apparatus, identifications of compounds were made by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Seventy seven different compounds were identified from essential oils of the two different khat cultivars. The essential oils in the samples from Bahir Dar and Wendo were composed of 50 and 34 compounds, respectively. The major compound identified in khat essentials oils include: limonene, 1-phenyl-1,2-propanedione, 1-hydroxy,1-phenyl-2-propanone, camphor, (sulfurous acid)-2-propylundecyl ester, hexadecane, O-mentha-1(7), 8-dien-3-ol, heptadecane, 10-methylnonadecane, (phthalic acid)-isobutyl octadecyl ester, and tritetracontane. The antioxidant and free radical scavenging activity of the oils were assessed by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activities of the oils were 23.5-23.6 μg AAE/kg of fresh khat sample.

Chemical variability and antioxidant activities of the essential oils of the aerial parts of Ammoides verticillata and the roots of Carthamus caeruleus and their synergistic effect in combination

2020 ◽  
Vol 23 ◽  
Author(s):  
Imane Rihab Mami ◽  
Noria Merad-Boussalah ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Food ◽  
Chemical Compositions ◽  
Chemical Variability ◽  
Ammoides Verticillata

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

scholarly journals Antioxidant Activity and Chemical Composition of Essential Oils of some Aromatic and Medicinal Plants from Albania

2017 ◽  
Vol 12 (5) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Entela Hodaj-Çeliku ◽  
Olga Tsiftsoglou ◽  
Lulëzim Shuka ◽  
Sokol Abazi ◽  
Dimitra Hadjipavlou-Litina ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stable Radical ◽  
In Vitro Assays ◽  
Bornyl Acetate ◽  
Soybean Lipoxygenase ◽  
Chemical Compositions ◽  
Caryophyllene Oxide

The chemical compositions have been investigated of the volatile oils of nine populations of six species from Albania, namely Artemisia absinthium, Calamintha nepeta, Hypericum perforatum, Sideritis raeseri subsp. raeseri, Origanum vulgare subsp. hirtum from two wild populations, and Salvia officinalis (sage) from two wild and one cultivated population,. The essential oils were obtained by hydrodistillation and their analyses were performed by GC–MS. The major constituents were: A. absinthium: neryl isovalerate (19.5%), geranyl isobutanoate (16.4%) and carvacrol (8.8%); C. nepeta: pulegone (31.7%), spathulenol (20.0%) and isomenthone (12.7%); H. perforatum: caryophyllene oxide (31.0%), δ-selinene (10.5%) and carvacrol (10.4%); O. vulgare: carvacrol (81.0, 78.6%), γ-terpinene (5.5, 7.1%) and p-cymene (4.9, 4.1%) for O. vulgare originating from Tepelena and Vlora, respectively; S. raeseri: carvacrol (36.7%), caryophyllene oxide (17.8%), β-caryphyllene (8.7%), spathulenol (7.7%) and myrtenol (6.4%); S. officinalis: camphor (40.2, 47.8, 45.9%), α-thujone (19.2, 22.2, 13.7%), eucalyptol (5.4, 2.6, 6.0%), camphene (5.8, 6.1, 3.9, %), borneol (2.1, 2.9, 5.7%) and bornyl acetate (3.3, 1.4, 5.6%) for samples originating from Tepelena, Tirana and Vlora, respectively. The essential oils were also tested for their free radical scavenging activity using the following in vitro assays: i) interaction with the free stable radical of DPPH (1,1-diphenyl-2-picrylhydrazyl), and ii) inhibition of linoleic acid peroxidation with 2,2'-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH). Finally, their inhibitory activity toward soybean lipoxygenase was evaluated, using linoleic acid as substrate. The essential oil of O. vulgare (OV-VL) presented the highest interaction with the stable radical DPPH (76.5%), followed by that of A. absinthium (54.7%). O. vulgare (OV-TP) and A. absinthium showed high anti-lipid peroxidation activity, 97.5% and 96.5%, respectively, higher than that of the reference compound trolox (73.0%). Only the tested sample of O. vulgare (OV-VL) significantly inhibited soybean lipoxygenase (54.2%).

Evaluating the Effects of Microwave-Assisted Hydrodistillation on Antifungal and Radical Scavenging Activities of Oliveria decumbens and Chaerophyllum macropodum Essential Oils

2017 ◽  
Vol 80 (5) ◽  
pp. 783-791 ◽  
Author(s):  
Nastaran Khajehie ◽  
Mohammad-Taghi Golmakani ◽  
Marzieh Eblaghi ◽  
Mohammad Hadi Eskandari
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity ◽  
Minimum Fungicidal Concentration ◽  
Spectrometry Analysis ◽  
Microwave Assisted

ABSTRACT In this study, radical scavenging and antifungal activities of Chaerophyllum macropodum and Oliveria decumbens essential oils (EOs) extracted with microwave-assisted hydrodistillation (MAHD) were evaluated in comparison with the same EOs extracted by conventional hydrodistillation (HD). The final EO yields that were obtained using HD (after 150 min) and MAHD (after 45 min) were 1.72 and 1.67% for C. macropodum and 8.10 and 7.91% for O. decumbens, respectively. There were no significant differences between the final EO yields extracted with HD and MAHD, but MAHD could significantly reduce the duration of the extraction operation. Average rates of EO accumulation (grams per minute) with MAHD were at least three times higher than with HD. Gas chromatography–mass spectrometry analysis of EOs indicated that there were no significant differences between the composition of EOs extracted by HD and MAHD. Both plants showed high radical scavenging activity, with 50% inhibitory concentration values of 0.430 to 0.431 mg/mL for C. macropodum and 0.142 to 0.146 mg/mL for O. decumbens. Antifungal activity was performed against six fungal species, including Aspergillus niger, Aspergillus oryzae, Penicillium chrysogenum, Trichoderma harzianum, Byssochlamys spectabilis, and Paecilomyces variotii. A. niger and A. oryzae were the most resistant fungi, and T. harzianum was the most susceptible. Evaluation of MIC and minimum fungicidal concentration values showed that the O. decumbens EOs were very active against all the tested fungi, which can be attributed to the high amounts of oxygenated terpenes in the EO content. Therefore, MAHD as a fast extraction technique did not have any adverse effects on chemical composition, radical scavenging activity, and antifungal activity of C. macropodum and O. decumbens EOs.

scholarly journals Biological Activities and Chemical Constituents of Essential Oils from Piper cubeba Bojer and Piper nigrum L.

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1876 ◽  
Author(s):  
Yusuf Andriana ◽  
Tran Dang Xuan ◽  
Tran Ngoc Quy ◽  
Hoang-Dung Tran ◽  
Quang-Tri Le
Keyword(s):  
Mass Spectrometry ◽  
Essential Oils ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Herbicidal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Chemical Compositions ◽  
Ionization Mass

In this study, we evaluated antioxidant, antihyperuricemic, and herbicidal activities of essential oils (EOs) from Piper cubeba Bojer and Piper nigrum L.; two pepper species widely distributed in tropics, and examined their chemical compositions. Dried berries of P. cubeba and P. nigrum were hydro-distilled to yield essential oil (EO) of 1.23 and 1.11% dry weight, respectively. In the antioxidant assay, the radical scavenging capacities of P. cubeba EO against DPPH and ABTS free radicals were 28.69 and 24.13% greater than P. nigrum, respectively. In the antihyperuricemic activity, P. cubeba EO also exhibited stronger inhibitory effects on xanthine oxidase (IC50 = 54.87 µg/mL) than P. nigrum EO (IC50 = 77.11 µg/mL). In the herbicidal activity, P. cubeba EO showed greater inhibition on germination and growth of Bidens pilosa and Echinochloa crus-galli than P. nigrum EO. Besides, P. cubeba EO decreased 15.98–73.00% of photosynthesis pigments of B. pilosa and E. crus-galli, while electrolyte leakages, lipid peroxidations, prolines, phenolics, and flavonoids contents were increased 10.82–80.82% at 1.93 mg/mL dose. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that P. nigrum and P. cubeba EOs principally possessed complex mixtures of monoterpenes and sesquiterpenes. Terpinen-4-ol (42.41%), α-copaene (20.04%), and γ-elemene (17.68%) were the major components of P. cubeba EO, whereas β-caryophyllene (51.12%) and β-thujene (20.58%) were the dominant components of P. nigrum EO. Findings of this study suggest both P. cubeba and P. nigrum EOs were potential to treat antioxidative stress and antihyperuricemic related diseases. In addition, the EOs of the two plants may be useful to control B. pilosa and E. crus-galli, the two invasive and problematic weeds in agriculture practice.

scholarly journals Chemo-typing of Three Egyptian Rosemary Oil for Their Chemical Composition and Anti-oxidant Activity

2020 ◽  
pp. 9-14
Author(s):  
Menna I. Elshorbagy ◽  
Marwa Elsbaey ◽  
Hany N. Baraka ◽  
Mohamed Farid Lahloub
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Standard Procedure ◽  
Radical Scavenging ◽  
Volatile Oil ◽  
Fused Silica ◽  
Dpph Assay

Aims: To study the chemical composition of volatile oil samples from different place in Egypt (Mansoura, Gamsa and Assuit) and study their antioxidant activity determined by DPPH assay. Study Design: Hydro distillation of volatile oil samples and their GC/MS analysis and determination of their antioxidant activity by DPPH assay. Place and Duration of Study: Department of Pharmacognosy, Faculty of pharmacy, Mansoura university, Egypt, between June 2015 and November 2017.   Methodology: The essential oil was isolated by hydro-distillation for 5 h using a Clevenger-type all-glass apparatus according to the standard procedure of the European pharmacopeia and volatile oils analysis was performed by GC and GC-MS. GC analysis was carried out using Focus-DSQ-ӀI GC/MS instrument (Thermo Scientific, MA, USA) equipped with TR-5 fused silica column (30 m × 0.25 mm, film thickness 0.25 µm). Results: The yield of the essential oils of three rosemary plants growing in Mansoura (RM), Gamsa (RG) and Assiut (RA) were 0.20% v/w, 0.32% v/w and 0.24% v/w, respectively. Their chemical composition was analyzed by GC/MS, RM and RG were found to be α-pinene dominated chemotypes, 32.4% and 29.6%, respectively, meanwhile RA was camphor dominated chemotype (17.2%). Furthermore, their antioxidant activity was determined by DPPH assay. Their IC50 values of essential oils of RM, RG and RA were 8.66 ± 0.7, 8.18 ± 0.5 and 9.74 ± 0.2, respectively. Conclusion: The GC/MS spectral data revealed the considerable difference between the chemical composition of essential oil constituents of RM, RG and RA which lead to different chemotypes. The present results also demonstrate that REO obtained from different areas in Egypt exhibited free radical scavenging activity determined by DPPH assay due to the synergistic effect between their constituents.

scholarly journals A Comparative Study of Antimicrobial and Antioxidant Activities of Plant Essential Oils and Extracts as Candidate Ingredients for Edible Coatings to Control Decay in ‘Wonderful’ Pomegranate

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3367
Author(s):  
Tatenda Gift Kawhena ◽  
Umezuruike Linus Opara ◽  
Olaniyi Amos Fawole
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Grape Pomace ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Edible Coatings ◽  
Penicillium Spp ◽  
Paper Disc

This study determined the antimicrobial and antioxidant activity of lemongrass (LO), thyme (TO), and oregano (OO) essential oils and ethanolic extracts of pomegranate peel (PPE) and grape pomace (GPE) as candidate ingredients for edible coatings. Antifungal effects against Botrytis cinerea and Penicillium spp. were tested using paper disc and well diffusion methods. Radical scavenging activity (RSA) was evaluated using 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid assays. Gas chromatography-mass spectrometry analysis identified limonene (16.59%), α-citral (27.45%), β-citral (27.43%), thymol (33.31%), paracymene (43.26%), 1,8-cineole (17.53%), and trans-caryphellene (60.84%) as major compounds of the essential oils. From both paper disc and well diffusion methods, LO recorded the widest zone of inhibition against tested microbes (B. cinerea and Penicillium spp.). The minimum inhibitory concentrations of LO against B. cinerea and Penicillium spp., were 15 µL/mL and 30 µL/mL, respectively. The highest (69.95%) and lowest (1.64%) RSA at 1 mg/mL were recorded for PPE and OO. Application of sodium alginate and chitosan-based coatings formulated with LO (15 or 30 µL/mL) completely inhibited spore germination and reduced the decay severity of ‘Wonderful’ pomegranate. Lemongrass oil proved to be a potential antifungal agent for edible coatings developed to extend shelf life of ‘Wonderful’ pomegranate.

scholarly journals Composition and Bioactivities of an (E)-β-Farnesene Chemotype of Chamomile (Matricaria Chamomilla) Essential Oil from Nepal

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Prabodh Satyal ◽  
Samon Shrestha ◽  
William N. Setzer
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Essential Oil ◽  
Breast Tumor ◽  
Artemia Salina ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Matricaria Chamomilla ◽  
Breast Tumor Cells ◽  
Mcf 7

The essential oil of Matricaria chamomilla, collected from Nepal, was obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. The major components in Nepalese chamomile oil were ( E)-β-farnesene (42.2%), α-bisabolol oxide A (22.3%), ( E,E)-α-farnesene (8.3%), cis-bicycloether (5.0%), α-bisabolol oxide B (4.5%), and α-bisabolone oxide A (4.0%). A cluster analysis based on the chemical compositions of 48 samples of chamomile oil reported in the literature has revealed seven chemotypes, and the oil from Nepal represents the ( E)-β-farnesene chemotype. The chamomile oil was screened for antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger, and toxicity toward MCF-7 breast tumor cells, Artemia salina, Chaoborus plumicornis, Caenorhabditis elegans, and Drosophila melanogaster.

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