scholarly journals Metabolic Analysis of Vigna unguiculata Sprouts Exposed to Different Light-Emitting Diodes

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Hyeon Ji Yeo ◽  
Chang Ha Park ◽  
Kyoung Bok Lee ◽  
Jae Kwang Kim ◽  
Jong Seok Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Vigna Unguiculata ◽  
High Performance ◽  
Light Emitting Diodes ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Total Carotenoids ◽  
Light Emitting ◽  
Tof Ms

In order to determine the effect of light-emitting diodes (LEDs) on plant metabolism, the present study examined the primary and secondary metabolite profiles of Vigna unguiculata L. Walp. sprouts that were exposed to red, blue, white, or a combination of red and blue LEDs using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). A total of 39 hydrophilic compounds were identified and quantitated using GC-TOF-MS, and six phenylpropanoids and six carotenoids were quantified using HPLC. The plants grown under blue LED light contained the highest level of total carotenoids (253.72 ± 17.27 μg/g) and phenylpropanoids (2600.51 ± 4.90 μg/g). Thus, the current study provides a new approach for enhancing the carotenoid and phenylpropanoid production of V. unguiculata.

scholarly journals Evaluation of Metabolites and Antioxidant Activity in Pumpkin Species

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2092098 ◽  
Author(s):  
Hafiz Muhammad Khalid Abbas ◽  
He-Xun Huang ◽  
Wen-Jie Huang ◽  
Shu-Dan Xue ◽  
Shi-Juan Yan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Cucurbita Moschata ◽  
Cucurbita Maxima ◽  
Total Carotenoids ◽  
Β Carotene

Cucurbita species ( Cucurbita moschata and Cucurbita maxima) are rich sources of nutrients, especially carotenoids and carbohydrates. Metabolites in pulps of C. moschata and C. maxima lines were examined by high-performance liquid chromatography and gas chromatography–mass spectrometry. Our results revealed that glucose and sucrose were the dominant sugars in C. maxima and C. moschata, respectively. A correlation was found between Brix percentage and sucrose levels as compared with glucose and fructose. A greater amount of myo-inositol had accumulated in C. moschata lines compared with C. maxima. Conversely, total carotenoids and antioxidant activity were found to be higher in C. maxima lines than in C. moschata. A strong correlation of glucose, fructose, and sucrose with β-carotene and violaxanthin revealed that it will be difficult to develop pumpkin cultivars with both high inositol and carotenoid levels. In conclusion, the composition of carbohydrates and carotenoids was more diverse in C. moschata lines than in C. maxima lines. Our results will contribute to a better understanding of metabolite changes in the fruits of these as well as other pumpkin species.

scholarly journals Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry and High-Sensitivity Gas Chromatography-Mass Spectrometry Screening of Classic Drugs and New Psychoactive Substances and Metabolites in Urine of Consumers

2021 ◽  
Vol 22 (8) ◽  
pp. 4000
Author(s):  
Emilia Marchei ◽  
Maria Alias Ferri ◽  
Marta Torrens ◽  
Magí Farré ◽  
Roberta Pacifici ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Sensitivity ◽  
Gas Chromatography Mass Spectrometry ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Resolution Mass

The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2.1 mm, 2.6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services.

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