scholarly journals The First Report on Chemical Composition and Antimicrobial Activity of Artemisia scoparia Waldst. et Kit. Extracts

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091503 ◽  
Author(s):  
Gordana S. Stojanović ◽  
Jovana D. Ickovski ◽  
Aleksandra S. Đorđević ◽  
Goran M. Petrović ◽  
Katarina D. Stepić ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Volatile Components ◽  
Bacterial Strains ◽  
First Report ◽  
Main Compound ◽  
Artemisia Scoparia

Volatiles of diethyl ether extract (DE), ethyl acetate extract (EE), and hexane extract (HE) of Artemisia scoparia Waldst. et Kit. were analyzed by gas chromatography with flame ionization detector and gas chromatography-mass spectrometry. In both DE and EE, the main compound was scoparone (24.0% and 86.1%, respectively) while in the HE, alkanes were dominant with nonacosane as the most represented (19.4%). Antimicrobial activity was tested against 4 bacterial strains and 1 fungal strain using disc-diffusion method. Tested samples were inactive against Gram-negative bacteria and they exhibited activity against Gram-positive bacteria and yeast Candida albicans. This is the first report on the chemical composition of volatile components and antimicrobial activity of DE, EE, and HE of A. scoparia Waldst. et Kit.

scholarly journals Biological activity of essential oil from Foeniculum vulgare

2021 ◽  
Vol 24 (2) ◽  
pp. 148-152
Author(s):  
Petra Borotová ◽  
Lucia Galovičová ◽  
Veronika Valková ◽  
Hana Ďúranová ◽  
Nenad Vuković ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Vapour Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Volatile Components ◽  
Foeniculum Vulgare ◽  
Disk Diffusion Method ◽  
Bacterial Growth Inhibition

Abstract Foeniculum vulgare Mill. is a medicinal plant, used as a flavouring agent. The essential oil from F. vulgare has potential antimicrobial and insecticidal effects, and can be used in food industry in order to protect the food resources and food products against microbial and pest’s contamination. The aim of the research was to characterize the volatile components of F. vulgare essential oil by Gas Chromatography/Mass Spectrometry (GC/MS) and Gas Chromatography (GC-FID) and to observe the antimicrobial activity by disk diffusion method and in vapour phase. Also, insecticidal activity of the vapour phase of the essential oil of F. vulgare was detected. We found that major components of the essential oil from F. vulgare were trans-anethole (73.6%), fenchone (6.0%), and limonene (5.7%). Antimicrobial activity on gram-positive, gram-negative, and yeasts was weak in liquid phase, but vapour phase showed stronger activity against B. subtilis at the concentration 250 μL.L−1 (98.65% of bacterial growth inhibition). Vapour phase of essential oil was effective against insects, where 25% concentration had 80% lethality.

scholarly journals Chemical composition of endemic Centaurea austro-anatolica and studies of its antimicrobial activity against multi-resistant bacteria

2009 ◽  
Vol 59 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Aysel Ugur ◽  
Nurdan Sarac ◽  
Ozgur Ceylan ◽  
M. Duru
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Aerial Parts ◽  
Multiresistant Bacteria ◽  
Paper Disc

Chemical composition of endemicCentaurea austro-anatolicaand studies of its antimicrobial activity against multi-resistant bacteriaHexane, chloroform, ethyl acetate and ethanolic extracts of the aerial parts ofCentaurea austro-anatolicaHub.-Mor. (Asteraceae) were evaluated against microorganisms, including multiresistant bacteria, using a paper disc diffusion method. The chloroform extract exhibited significant antibacterial activity toward all bacteria tested. The chemical composition of the chloroform extract was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major compounds of the extract were caryophyllene oxide (21.32 %), spathulenol (10.86 %),n-tricosanol (9.58 %) and geranyl isovalerate (8.71 %).

scholarly journals Chemical Composition, Enantiomeric Distribution, and Biological Activities of Rhododendron anthopogon Leaf Essential Oil from Nepal

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Noura S. Dosoky ◽  
Prabodh Satyal ◽  
Suraj Pokharel ◽  
William N. Setzer
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Cytotoxic Activities ◽  
Blood Disorders ◽  
Leaf Essential Oil ◽  
Microbroth Dilution

Rhododendron anthopogon D. Don., a small compact Himalayan shrub growing in Nepal, is a known medicinal plant used to treat sore throat, colds, blood disorders, bone disease, potato allergies, and vomiting, and to relieve liver disorders, headaches and back pain. The present study investigated the chemical composition and bioactivities of the leaf essential oil from R. anthopogon from Dhankuta, Nepal. The essential oil from leaves was obtained by hydrodistillation and a detailed chemical analysis was conducted by gas chromatography – mass spectrometry (GC-MS). The enantiomeric distribution of monoterpenoid components was determined using chiral gas chromatography and represents the first chiral examination of R. anthopogon essential oil. The essential oil was screened for antimicrobial activity using the microbroth dilution test, and for cytotoxic activity against MCF-7, MDA-MB-231, and 5637 using the MTT assay. A total of 70 volatile components were identified from the essential oil. The major components were α-pinene (21.5%), δ-cadinene (13.8%), β-pinene (9.5%), limonene (5.9%), δ-amorphene (4.6%), α-muurolene (4.5%), and ( E)-caryophyllene (3.2%) with other minor constituents (< 3%). The essential oil showed marginal antibacterial and cytotoxic activities, but no antifungal effects.

scholarly journals Antimicrobial Activity and Chemical Composition of the Essential Oils of Portuguese Foeniculum vulgare Fruits

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Ana S. Mota ◽  
M. Rosário Martins ◽  
Sílvia Arantes ◽  
Violeta R. Lopes ◽  
Eliseu Bettencourt ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Foeniculum Vulgare ◽  
Serovar Typhimurium ◽  
Food Spoilage Fungi

The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of Portugal. Composition of essential oils was established by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The obtained yields of the essential oils were found to vary greatly in the range of 1.1 to 2.9% (v/w) and the chemical composition varied with the region of collection. A total of 16 compounds were identified. The main compounds were fenchone (16.9 – 34.7%), estragole (2.5 – 66.0%) and trans-anethole (7.9 – 77.7%). The percentages of these three main compounds were used to determine the relationship between the different oil samples and to group them into four different chemotypes: anethole/fenchone; anethole; estragole and anethole/estragole. Antifungal activity of essential oils was evaluated against six food spoilage fungi: Aspergillus niger, A. japonicus, A. oryzae, Fusarium oxysporum, Rhizophus oryzae and R. stolonifer. Antibacterial activity was assessed against three Gram-positive strains: Enterococcus faecalis ATCC 29212, Staphylococcus epidermidis ATCC 12228 and S. aureus ATCC 28213; and against six Gram-negative strains: Escherichia coli ATCC 25922; Morganella morganii LFG 08; Proteus mirabilis LFG 04; Salmonella enteritidis LFG 05; S. entiritidis serovar typhimurium LFG 06 and Pseudomonas aeruginosa ATCC 27853 by the disc diffusion agar method; the minimal inhibitory concentration (MIC) was determined using the broth macro-dilution method. The MIC values varied from 62.5 ( E. coli ATCC 25922) to 2000 μg/mL ( P. aeruginosa ATCC 27853).

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

2017 ◽  
Vol 10 (11) ◽  
pp. 109
Author(s):  
Krishnavignesh L Krishnavignesh ◽  
Mahalakshmipriya A ◽  
Ramesh M
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

  Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

Chemical composition and biological activities of leaf and fruit essential oils from Eucalyptus camaldulensis

2017 ◽  
Vol 72 (11-12) ◽  
pp. 483-489 ◽  
Author(s):  
Gulden Dogan ◽  
Nazan Kara ◽  
Eyup Bagci ◽  
Seher Gur
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Biological Activities ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Leaf Oil ◽  
Fruit Oil ◽  
Antibacterial And Antifungal

AbstractThe chemical composition of the essential oils from the leaves and fruit ofEucalyptus camaldulensisgrown in Mersin, Turkey was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. The main leaf oil constituents werep-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). The main constituents of the fruit oil were eucalyptol (1,8-cineole) (34.5%),p-cymene (30.0%), α-terpinol (15.1%) and α-pinene (9.0%). Our results showed that both types of oils are rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. The leaf and fruit essential oils ofE. camaldulensissignificantly inhibited the growth of Gram-positive (Staphylococcus aureusandBacillus subtilis) and Gram-negative (Escherichia coliandStreptococcussp.) bacteria (p<0.05). The oils also showed fungicidal activity againstCandida tropicalisandC. globrata. Leaf essential oils showed more activity than fruit essential oils, probably due to the higher p-cymene concentration in leaves.

scholarly journals Antimicrobial Activity and Analysis of the Essential Oils of Selected Endemic Edible Apiaceae Plants Root from Caspian Hyrcanian Region (North of Iran)

2019 ◽  
Vol 25 (2) ◽  
pp. 138-144
Author(s):  
Azadeh Hamedi ◽  
Ardalan Pasdaran ◽  
Arsalan Pasdaran
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fatty Acid Esters ◽  
Herbal Remedies ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Root Essential Oil

Background: Different endemic species of Apiaceae that widely grow in Hyrcanian region have long been used as spices and herbal remedies. Chemical compositions and bioactivity of many of these plants have not been studied scientifically. The volatile constituents and antimicrobial activity of four edible Apiaceae plants—Elaeosticta glaucescens Boiss, Malabaila secacul (Mill.) Boiss, Caucalis platycarpos L., and Eryngium caucasicum Trautv—roots were investigated. Methods: Gas chromatography/mass spectrometry (GC–MS) and gas chromatography/flame ionization detection (GC–FID) methods were used for chemical investigation. Antimicrobial potential of the volatile compositions of these roots were investigated using the disc diffusion method on four microorganisms including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis. Results: The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these species were revealed for B. subtilis and S. aureus (500 µg/mL for MIC and MBC) followed by E. caucasicum and C. platycarpus, respectively. The main compounds of E. caucasicum root essential oil were hexyl isovalerate (11.0%) and hexyl valerate (10.1%). E. glaucescens root essential oil was mainly composed of 3(10)-caren-4-ol, acetoacetic acid ester (9.8%), octyl isovalerate (8.9%), and octyl acetate (5.7%). In C. platycarpos root, hexyl isovalerate (9.2%) and n-octyl isobutyrate (8.5%) were constituted the major compositions of the essential oil while n-hexyl isobutyrate (8.5%) and hexyl hexanoate (7.8%) were predominant compounds in M. secacul. Conclusion: The essential oils of E. caucasicum and C. platycarpus roots were contained high amount of short chain fatty acid esters (SCFAE) and trans- pinocarvyl acetate. They exhibited moderate antibacterial activity.

scholarly journals Chemical composition and antimicrobial activity of extracts from thyme and rosemary against Staphylococcus aureus and Candida albicans

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9656-9671
Author(s):  
Akram Sabzikar ◽  
Seyyed Khalil Hosseinihashemi ◽  
Younes Shirmohammadli ◽  
Abbas Jalaligoldeh
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Rosemary Extract ◽  
Gram Positive Bacteria ◽  
Main Components

Different concentrations of ethanolic extracts of thyme (Zataria multiflora) and rosemary (Rosmarinus officinalis) were evaluated to determine their antimicrobial activity using the agar-well diffusion method. The values of inhibition zone diameter (IZD) for Candida albicans fungus and Staphylococcus aureus Gram-positive bacteria were determined. The bioactivities of two various extracts were studied, and the chemical composition of the extracts were identified using gas chromatography-mass spectrometry (GC-MS) technique. The results of the test showed that at concentrations of 10% and 40% thyme extract, the values of IZD were 12.5 mm and 23.3 mm, respectively, against the growth of S. aureus, which were higher than C. albicans (7.0 mm and 22.5 mm, respectively). The rosemary extract at concentrations of 20% and 60% showed lower antibacterial activity against S. aureus (4.7 mm and 8.7 mm IZD, respectively) and lower antifungal activity against C. albicans (12.2 mm and 1.7 mm IZD, respectively). At a concentration of 40% thyme extract, the highest antibacterial (23.3 mm IZD) and antifungal (22.5 mm IZD) activities were observed. The GC/MS analysis showed that carvacrol (52.3%), linalool L (16%), and thymol (9.6%) were the main components of thyme extract, while in the rosemary extract β-amyrone (18.0%), verbenone (8.0%), and 1,8-cineole (7.26%) were the major constituents.

scholarly journals CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS OF FOUR VARIETIES OF LIPPIA MULTIFLORA IN BENIN

2019 ◽  
Author(s):  
GANDONOU Dossa Clément ◽  
BAMBOLA Bouraïma ◽  
TOUKOUROU Habib ◽  
GBAGUIDI Ahokannou Fernand ◽  
DANSOU Christian ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Lippia Multiflora ◽  
Natural Substances

Present study involves the study of the chemical composition of the essential oils extracted from the leaves by gas chromatography and gas chromatography coupled with mass spectrometry of Lippia multiflora harvested in the regions of Kétou, Savalou, Bohicon and Mono and tested by the well diffusion method against pathogenic microorganisms. The essential oils studied are terpene compounds, aromatic compounds, aliphatic compounds and other natural substances. The inhibition zone diameters determined allowed us to evaluate their degree of germ sensitivity of the strains tested. Essential oils extracted from Lippia multiflora harvested in these areas have the most pronounced antimicrobial activity. In total, the essential oils tested have different and specifically a degree of sensitivity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis A24974, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa except that harvested in the Savalou who does not have no degree of sensitivity on Pseudomonas aeruginosa. This work paves the way for food preservation with extracted natural substances and also the formulation of natural antimicrobials for this fact.

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