scholarly journals Novel 1,3-Benzodioxole From Marine-Derived Actinomycete in East Vietnam Sea

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092004
Author(s):  
Dao Phi Thi ◽  
Huong Doan Thi Mai ◽  
Duc Danh Cao ◽  
Quyen Vu Thi ◽  
Mai Anh Nguyen ◽  
...  
Keyword(s):  
2D Nmr ◽  
Culture Broth ◽  
Genus Streptomyces ◽  
Nmr Data ◽  
Actinomycete Strain ◽  
Spectral Data Analysis ◽  
And Staphylococcus Aureus ◽  
16Srrna Gene ◽  
Mcf 7 ◽  
C Nmr

Analysis of an antimicrobial extract from the culture broth of the marine-derived actinomycete Streptomyces sp. G261 led to the isolation of a new 1,3-benzodioxole derivative (1), together with 10 known compounds 2-11. The actinomycete strain G261 was isolated from sediment, collected at Cu Lao Cham, Quang Nam in Vietnam. The taxonomic identification of the strain G261 was achieved by analysis of 16SrRNA gene sequences. On the basis of morphological and phylogenetic evidence, the actinomycete strain G261 was assigned to the genus Streptomyces. The structures of the isolated compounds were established by their spectral data analysis, including mass spectrometry, 1-dimensional nuclear magnetic resonance (1D-NMR), and 2D-NMR. The structure of 1 was confirmed by comparison of the calculated with experimental 13C NMR data. Compound 1 exhibited antimicrobial activity against Enterococcus faecalis and Staphylococcus aureus with minimum inhibitory concentration values of 128 and 256 µg/mL, respectively. Whereas, compound 1 had a weak inhibition when tested against 4 cancer cell lines, KB, LU-1, HepG-2, and MCF-7.

scholarly journals Secondary Metabolites from an Actinomycete from Vietnam's East Sea

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Quyen Vu Thi ◽  
Van Hieu Tran ◽  
Huong Doan Thi Mai ◽  
Cong Vinh Le ◽  
Minh Le Thi Hong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
2D Nmr ◽  
Culture Broth ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Nmr Data ◽  
Clinically Significant ◽  
First Time ◽  
Mcf 7

Analysis of an antimicrobial extract prepared from culture broth of the marine-derived actinomycete Nocardiopsis sp. (strain G057) led to the isolation of twelve compounds, 1–12. Compound 1 (2-[(2 R-hydroxypropanoyl)amino]benzamide) was found to be a new enantiomeric isomer while compounds 2 (3-acetyl-4-hydroxycinnoline) and 3 (3,3′-bis-indole) were isolated from a natural source for the first time. The structures of 1 – 12 were determined by analyses of MS and 2D NMR data. All compounds were evaluated for their antimicrobial activity against a panel of clinically significant microorganisms. Compound 1 selectively inhibited Escherichia coli (MIC: 16 μg/mL). Compounds 2 and 3 exhibited antimicrobial activity against several strains of both Gram-positive and Gram-negative bacteria, and the yeast Candida albicans. Cytotoxic evaluation of compounds 1 – 3 against four cancer cell lines (KB, LU-1, HepG-2 and MCF-7) indicated that compound 3 produced a weak inhibition against KB and LU cell lines. Two remaining compounds, 1 and 2 were not cytotoxic, even at the concentration of 128 μg/mL.

scholarly journals Flavonoids from Curcuma longa Leaves and their NMR Assignments

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Chia-Ling Jiang ◽  
Sheng-Fa Tsai ◽  
Shoei-Sheng Lee
Keyword(s):  
Nmr Assignments ◽  
Soluble Fraction ◽  
Curcuma Longa ◽  
2D Nmr ◽  
Etoh Extract ◽  
Spectroscopic Analyses ◽  
Nmr Data ◽  
The Common ◽  
Reported Data ◽  
C Nmr

Chemical investigation of the n-BuOH-soluble fraction of the EtOH extract of the aerial part of Curcuma longa led to the isolation of 11 flavonol glycosides and one dihydroflavonol glucoside (1) via chromatography over Sephadex LH-20 and Lobar RP-18 columns. Although they are known, the 1H and 13C NMR data recorded in CD3OD rather than the common DMSO- d6 are doubly checked via extensive 2D NMR spectroscopic analyses, leading to some revisions of the reported data, especially for the glycon part.

Dammarane and Ceanothane Triterpenes from Zizyphus glabrata

2006 ◽  
Vol 61 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Seru Ganapaty ◽  
Pannakal S. Thomas ◽  
Kancharalapalli V. Ramana ◽  
Gloria Karagianis ◽  
Peter G. Waterman
Keyword(s):  
Bactericidal Activity ◽  
Chemical Constituents ◽  
2D Nmr ◽  
First Report ◽  
Nmr Data ◽  
C Nmr ◽  
Dammarane Glycoside

From the leaves of Zizyphus glabrata, a new dammarane-type triterpene, pseudojujubogenin -3-O- β -D-glucopyranoside, along with the known ceanothane triterpenes, granulosic acid, ceanothic acid and daucosterol were isolated. The structures of the compounds were fully characterized by detailed NMR investigations including 1H and 13C NMR, HSQC, COSY, HMBC and NOESY experiments. In addition, the dammarane glycoside was tested for its potential to inhibit various bacteria and was found to possess significant bactericidal activity. The 1H, 13C and full 2D-NMR data on granulosic acid has also been presented. This is the first report on the chemical constituents of the leaves of Z. glabrata.

scholarly journals Medelamine C, A New ω-Hydroxy Alkylamine Derivative from Endophytic Streptomyces sp. YIM 66142

2014 ◽  
Vol 9 (1) ◽  
pp. 1934578X1400900
Author(s):  
Ju-Cheng Zhang ◽  
Ya-Bin Yang ◽  
Hao Zhou ◽  
Tian-Feng Peng ◽  
Fang-Fang Yang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Biosynthetic Pathway ◽  
2D Nmr ◽  
Sw480 Cell ◽  
Hydroxyl Group ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Endophytic Streptomyces ◽  
Common Fatty Acid ◽  
Mcf 7

A new alkylamine derivative and a common fatty acid were isolated from Streptomyces sp. YIM 66142. On the basis of spectral data, including HRMS, NMR and 2D NMR, their structures were determined as medelamine C (1) and isomyristic acid (2). The ω-hydroxyl group in structure 1 is rare in a natural alkylamine. The possible biosynthetic pathway in the genus Streptomyces from isomyristic acid (2) to medelamines is proposed. Compound 1 showed no obvious cytotoxicity against HL-60, SMMC-7721, A-549, MCF-7, SW480 cell lines. The ω-hydroxyl and the acetyl at NH in compound 1 decreased its cytotoxicity in comparison with that of medelamine.

Alteration of O-specific polysaccharide structure of symbiotically defective Mesorhizobium loti mutant 2213.1 derived from strain NZP2213.

2008 ◽  
Vol 55 (1) ◽  
pp. 191-200 ◽  
Author(s):  
Anna Turska-Szewczuk ◽  
Hubert Pietras ◽  
Wojciech Borucki ◽  
Ryszard Russa
Keyword(s):  
Wild Type Strain ◽  
2D Nmr ◽  
Chain Structure ◽  
Wild Type ◽  
Immunoblot Assay ◽  
Mesorhizobium Loti ◽  
Nmr Data ◽  
Specific Polysaccharide ◽  
C Nmr

Mesorhizobium loti mutant 2213.1 derived from the wild-type strain NZP2213 by Tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant Lotus corniculatus (Turska-Szewczuk et al., 2007 Microbiol Res, in press). The inability of lipopolysaccharide (LPS) isolated from the mutant 2213.1 strain or de-O-acetylated LPS of the parental cells to inactivate phage A1 particles implicated alterations in the LPS structure. The O-specific polysaccharide of the mutant was studied by chemical analyses along with (1)H and (13)C NMR spectroscopy, which clearly confirmed alterations in the O-chain structure. 2D NMR data showed that the mutant O-polysaccharide consists of a tetrasaccharide repeating unit containing non-substituted as well as O-acetylated or O-methylated 6-deoxytalopyranose residues. Additionally, an immunogold assay revealed a reduced number of gold particles on the mutant bacteroid cell surface, which could result from both a diminished amount of an O-antigenic determinant in mutant LPS and modifications of structural epitopes caused by alterations in O-acetylation or O-methylation of sugar residues. Western immunoblot assay of alkaline de-O-acetylated lipophilic M. loti NZP2213 LPS showed no reactivity with homologous serum indicating a role of O-acetyl groups in its O-specificity.

scholarly journals Nervisides I–J: Unconventional Side-Chain-Bearing Cycloartane Glycosides from Nervilia concolor

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2599
Author(s):  
Thi-Ngoc-Mai Tran ◽  
Guillaume Bernadat ◽  
Dinh-Tri Mai ◽  
Van-Kieu Nguyen ◽  
Jirapast Sichaem ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cytotoxic Activity ◽  
2D Nmr ◽  
Side Chain ◽  
Tumor Cell Lines ◽  
Nmr Data ◽  
Cycloartane Glycosides ◽  
Whole Plants ◽  
Stereogenic Center ◽  
Mcf 7

Two new cycloartane glycosides, nervisides I–J, were isolated from Nervilia concolor whole plants. Their structures were unambiguously established by interpretation of their HRESIMS and 1D and 2D NMR data. These cycloartanes comprised a stereogenic center at C-24, the R configuration of which was assigned based on DFT-NMR calculations and the subsequent DP4 probability score. These compounds were tested for cytotoxicity against K562 and MCF-7 tumor cell lines, revealing mild cytotoxic activity.

Anwendungen der 2 D-NMR-Spektroskopie in der Organophosphorchemie Beispiel: L-Menthyldichlorphosphan / Applications of the 2D NMR Spectroscopy in the Organophosphorus Chemistry Example: L-Menthyldichlorophosphine

1982 ◽  
Vol 37 (12) ◽  
pp. 1661-1664 ◽  
Author(s):  
Martin Feigel ◽  
Gerhard Hägele ◽  
Axel Hinke ◽  
Gudrun Tossing
Keyword(s):  
Nmr Spectroscopy ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
H Nmr ◽  
Nmr Parameters ◽  
Nmr Data ◽  
Organophosphorus Chemistry ◽  
C Nmr

2D NMR ist used to determine the 1H NMR parameters of L-Menthyldichlorophosphine. 13C NMR data are given

scholarly journals Chlokamycin, a New Chloride from the Marine-derived Streptomyces sp. MA2-12

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Takashi Fukuda ◽  
Misaki Takahashi ◽  
Hiroaki Kasai ◽  
Kenichiro Nagai ◽  
Hiroshi Tomoda
Keyword(s):  
2D Nmr ◽  
Culture Broth ◽  
Jurkat Cells ◽  
Streptomyces Sp ◽  
Spectroscopic Analyses ◽  
Nmr Data ◽  
Hct116 Cells

A new chlorinated metabolite designated chlokamycin (1), was isolated along with ikarugamycin (2) from the culture broth of the marine-derived Streptomyces sp. MA2–12. The structure of 1 was elucidated based on spectroscopic analyses (1D and 2D NMR data and ROESY correlations). Chlokamycin moderately inhibited the growth of Jurkat cells and HCT116 cells with IC50 values of 24.7 and 33.5 μM, respectively.

scholarly journals Oleanane-type Triterpene Glycosides from Glycyrrhiza Uralensis

2007 ◽  
Vol 2 (3) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Jinwei Li-Yang ◽  
Jun-ichiro Nakajima ◽  
Nobuhito Kimura ◽  
Kazuki Saito ◽  
Shujiro Seo
Keyword(s):  
Natural Products ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Mass Spectrometric ◽  
Triterpene Glycosides ◽  
Glycyrrhiza Uralensis ◽  
Nmr Data ◽  
Nmr Methods ◽  
First Time ◽  
C Nmr

Chemical investigation of the roots of Glycyrrhiza uralensis resulted in the isolation of six oleanane-type triterpene glycosides (1 – 6), including one new saponin (1) and two (2 and 3) obtained as natural products for the first time. The new licorice saponin (1) was identified as 22β–acetoxylglycyrrhizin from [α]D, mass spectrometric, and UV, IR, and NMR spectroscopic data.. Full assignments of 1H and 13C-NMR data for compounds 2, 3, 4, 5 and 6 were made for the first time according to 2D NMR methods.

scholarly journals Two New Terpenoids from Trichilia quadrijuga (Meliaceae)

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500
Author(s):  
Virginia F. Rodrigues ◽  
Hadria M. Carmo ◽  
Raimundo Braz Filho ◽  
Leda Mathias ◽  
Ivo J. Curcino Vieira
Keyword(s):  
Data Analysis ◽  
Spectral Data ◽  
Mass Spectra ◽  
2D Nmr ◽  
Spectral Data Analysis ◽  
C Nmr

Two new terpenoids, ambrosanoli-10(14)-en-11,12-diol (1), a sesquiterpene named quadrijugol, and a pregnane steroid, 3β,4β-dihydroxypregnan-16-one (2), were isolated from the stem and leaves of Trichilia quadrijuga, along with eleven known compounds, spathulenol, kudtdiol, 2β,3β,4β-trihydroxypregnan-16-one, bourjotinolone B, piscidinol, niloticin, dihydroniloticin, β-sitosterol, 3-O-β-D-glucopyranosyl-sitosterol, itesmol and stigmasterol. Structures were elucidated by spectral data analysis, mainly afforded by 1H and 13C NMR (1D and 2D NMR HMQC, HMBC, NOESY and COSY) and mass spectra.

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