Activin A and Follistatin Serum Concentrations in Breast Augmentation Patients

2021 ◽  
pp. 229255032110511
Author(s):  
Philip H. Zeplin
Keyword(s):  
Breast Augmentation ◽  
Capsular Contracture ◽  
Serum Levels ◽  
Activin A ◽  
Control Group ◽  
Revision Operation ◽  
Blood Samples ◽  
Capsular Fibrosis ◽  
Aesthetic Breast

Background: Capsular contracture is caused by an excessive fibrotic reaction similar as observed in other progressive fibrotic disorders. For their pathogenesis, several studies confirmed the importance of activins and follistatin. The aim of this study was to determine and analyze serum levels of Activin A and follistatin in patients with capsular contracture after aesthetic breast augmentation. Methods: The study included 361 female patients who underwent primary aesthetic breast augmentation, came for control examination after breast augmentation or for revision operation because of capsular contracture. Blood samples were taken and using a specific ELISA to determine the serum concentration levels of Activin A and Follistatin. Results: Ninety-six patients (n = 96), who developed a capsular contracture Baker ≥°III and underwent revision surgery were collected (capsular fibrosis group). One-hundred and fourteen patients (n = 114) were asymptomatic for capsular fibrosis Baker ≥°III after primary breast augmentation and 33 (n = 33) of them had developed no capsular fibrosis after more than 10 years (long-term group). For control group, blood samples were taken from 167 patients (n = 167) before primary aesthetic breast augmentation. Serum Activin A levels were significantly higher in the long-term Group compared with those in the capsular fibrosis- and the control groups. Follistatin levels were significantly lower in the capsular fibrosis group compared to the control- and the long-term groups. A small amount of control group patients (n = 16) developed a capsular fibrosis within 2 years after primary breast augmentation with significant lower follistatin levels. Retrospectively, they showed significantly lower serum follistatin levels than the control group even before the onset of capsular contracture. Conclusions: Capsular fibrosis has no effect on Activin A serum levels. In contrast, follistatin serum levels are lower in patients with capsular fibrosis. These results show that besides many other factors, a dysregulation of the Activin–follistatin axis may have importance on the pathogenesis of capsular contracture.

scholarly journals Vitamin e-loaded membrane dialyzers reduce hemodialysis inflammaging

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Vincenzo Sepe ◽  
Marilena Gregorini ◽  
Teresa Rampino ◽  
Pasquale Esposito ◽  
Rosanna Coppo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vitamin E ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Control Group ◽  
Low Grade ◽  
Oxide Formation ◽  
Blood Samples ◽  
Indoleamine 2,3 Dioxygenase ◽  
Nitric Oxide Formation

Abstract Background Inflammaging is a persistent, low−grade, sterile, nonresolving inflammatory state, associated with the senescence of the immune system. Such condition downregulates both innate and adaptive immune responses during chronic disorders as type II diabetes, cancer and hemodialysis, accounting for their susceptibility to infections, malignancy and resistance to vaccination. Aim of this study was to investigate hemodialysis inflammaging, by evaluating changes of several hemodialysis treatments on indoleamine 2,3-dioxygenase-1 activity and nitric oxide formation. Methods We conducted a randomized controlled observational crossover trial. Eighteen hemodialysis patients were treated with 3 different hemodialysis procedures respectively: 1) Low−flux bicarbonate hemodialysis, 2) Low−flux bicarbonate hemodialysis with vitamin E − loaded dialyzers, and 3) Hemodialfitration. The control group consisted of 14 hospital staff healthy volunteers. Blood samples were collected from all 18 hemodialysis patients just after the long interdialytic interval, at the end of each hemodialysis treatment period. Results Hemodialysis kynurenine and kynurenine/L − tryptophan blood ratio levels were significantly higher, when compared to the control group, indicating an increased indoleamine 2,3-dioxygenase-1 activity in hemodialysis patients. At the end of the low−flux bicarbonate hemodialysis with vitamin E − loaded dialyzers period, L − tryptophan serum levels remained unchanged vs both low−flux bicarbonate hemodialysis and hemodialfitration. Kynurenine levels instead decreased, resulting in a significant reduction of kynurenine/L − tryptophan blood ratio and indoleamine 2,3-dioxygenase-1 activity, when matched to both low−flux bicarbonate hemodialysis and HDF respectively. Serum nitric oxide control group levels, were significantly lower when compared to all hemodialysis patient groups. Interestingly, low−flux bicarbonate hemodialysis with vitamin E − loaded dialyzers nitric oxide serum levels from venous line blood samples taken 60 min after starting the hemodialysis session were significantly lower vs serum taken simultaneously from the arterial blood line. Conclusions The treatment with more biocompatible hemodialysis procedure as low−flux bicarbonate hemodialysis with vitamin E − loaded dialyzers, reduced indoleamine 2,3-dioxygenase-1 activity and nitric oxide formation when compared to both low−flux bicarbonate hemodialysis and hemodialfitration. These data suggest that low−flux bicarbonate hemodialysis with vitamin E − loaded dialyzers lowering hemodialysis inflammaging, could be associated to changes of proinflammatory signalling a regulated molecular level. Trial registration NCT Number: NCT02981992; Other Study ID Numbers: 20100014090. First submitted: November 26, 2016. First posted: December 5, 2016. Last Update Posted: December 5, 2016.

scholarly journals The Effects of Formaldehyde on Cytochrome P450 Isoform Activity in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Min Xu ◽  
Huaqiao Tang ◽  
Qian Rong ◽  
Yuanli Zhang ◽  
Yinglun Li ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Plasma Concentrations ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
High Dose ◽  
Blood Samples ◽  
Time Points ◽  
Indoor Pollutant ◽  
Carcinogenic Effects

Formaldehyde (FA) is an occupational and indoor pollutant. Long-term exposure to FA can irritate the respiratory mucosa, with potential carcinogenic effects on the airways. The effects of acute FA poisoning on the activities of CYP450 isoforms CYP1A2, CYP2C11, CYP2E1, and CYP3A2 were assessed by determining changes in the pharmacokinetic parameters of the probe drugs phenacetin, tolbutamide, chlorzoxazone, and testosterone, respectively. Rats were randomly divided into three groups: control, low FA dose (exposure to 110 ppm for 2 h for 3 days), and high FA dose (exposure to 220 ppm for 2 h for 3 days). A mixture of the four probe drugs was injected into rats and blood samples were taken at a series of time points. Plasma concentrations of the probe drugs were measured by HPLC. The pharmacokinetic parameters t1/2, AUC(0-t), and Cmax of tolbutamide, chlorzoxazone, and testosterone increased significantly in the high dose versus control group (P<0.05), whereas the CL of chlorzoxazone and testosterone decreased significantly (P<0.05). However, t1/2, AUC(0-t), and Cmax of phenacetin decreased significantly (P<0.05), whereas the CL of phenacetin increased significantly (P<0.05) compared to controls. Thus, acute FA poisoning suppressed the activities of CYP2C11, CYP2E1, and CYP3A2 and induced the activity of CYP1A2 in rats. And the change of CYP450 activity caused by acute FA poisoning may be associated with FA potential carcinogenic effects on the airways.

scholarly journals Removal of fibroadenoma of the breast augmentation and breast implants. Analysis of the possibility of simultaneous and delayed execution of these operations

2021 ◽  
Vol 14 (2) ◽  
pp. 140-146
Author(s):  
Viktoriia Dzhuganova ◽  
Valery V. NOVOMLINSKY ◽  
Andrey Petrovich Sokolov ◽  
Pavel Alekseevich Lynov ◽  
Margarita Gennedievna Sokolova ◽  
...  
Keyword(s):  
Breast Augmentation ◽  
Capsular Contracture ◽  
Breast Implants ◽  
Needle Aspiration ◽  
Augmentation Mammoplasty ◽  
Simultaneous Treatment ◽  
Implant Placement ◽  
Simultaneous Operations ◽  
Cosmetic Effect

Introduction. Fibroadenomas (FA) are the most common benign breast neoplasms that are diagnosed in 25% of women. Dissatisfaction with the size of the breast and the desire to increase it occurs in 40%. For this reason, in the practice of a plastic surgeon, there are cases when the patient wants to remove fibroadenomas (FA) and increase the size of the breast. In this situation, there are two options for managing the patient- the simultaneous execution of two operations and the delayed one.Aim. To evaluate the possibility of simultaneous FA removal and augmentation mammoplasty, to analyze possible complications and methods of their correction.Materials and methods. We have analyzed the experience of simultaneous interventions of FA removal and augmentation mammoplasty on the example of 10 cases performed in the period from 2014-2019, as well as FA removal after implant placement-3 cases.Results. Performing a simultaneous operation has advantages due to the minimization of injuries (the ability to perform from a single access - submammary or periareolar), reducing psychological stress and better cosmetic effect. Two patients had postoperative complications in the form of capsular contracture, manifested in the asymmetry of the mammary glands, corrected by performing capsulotomy and forming a new submammary fold. When performing invasive diagnostic tests and surgical intervention in three patients after endoprosthesis augmentation mammoplasty, extreme caution was required due to the risk of violating the integrity of the implant. It was found that the incision of the posterior leaf of the MJ capsule with a large number of removed neoplasms in the postoperative period leads to the development of breast asymmetry. The fact of FA recurrence was also confirmed (2 patients), who subsequently underwent repeated surgical intervention.Conclusion. Performing simultaneous operations for benign breast tumors can be surely practiced by plastic surgeons, including as one of the options for simultaneous treatment of breast FA and augmentation mammoplasty. The occurrence of FA in the long-term period after breast augmentation surgery is associated with difficulties in diagnostics (mammography and fine needle aspiration biopsy under the control of ultrasound), as well as in the course of surgery itself, due to the presence of the implant and the risk of violation of its integrity.

scholarly journals Study the causes of growth retardation in pure breed imported calves after theileriosis in Abu-Shaeer cattle Farm /Diyala province

2013 ◽  
Vol 12 (2) ◽  
pp. 93
Author(s):  
Gh.H. Jameel
Keyword(s):  
Growth Hormone ◽  
Treatment Group ◽  
Group Treatment ◽  
Serum Levels ◽  
Infected Group ◽  
Control Group ◽  
Cattle Farm ◽  
Blood Samples ◽  
Mean Values ◽  
Significant Depression

The aim of this study is investigated the causes of retardation in growth of purebred calves infected by Theileria annulata.Blood samples of infected group (treatment group) were taken once, and blood samples of the control group (clinically healthy) were taken also .Serum separation was done to two groups to determinate some blood factors levels as ferritin ,phosphorus ,glucose ,growth hormone ,total T3 and total T4.It was detected that mean values of serum Ferritin was significantly higher than the values of the control group and healthy animals.No significant effect of the infection on the phosphorus and T3 levels ,While there were significant depression in serum levels of glucose ,growth hormone and T4.

scholarly journals Prevalence and Significance of Serum 14-3-3η in Juvenile Idiopathic Arthritis

2020 ◽  
Author(s):  
Iris Reyhan ◽  
Olga S. Zhukov ◽  
Robert J. Lagier ◽  
Robert F. Bridgeforth ◽  
Gary J. Williams ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Los Angeles ◽  
Disease Activity ◽  
Elevated Serum ◽  
Serum Levels ◽  
Control Group ◽  
Adult Rheumatoid Arthritis ◽  
Single Positive ◽  
Prompt Diagnosis

Abstract Background: Prompt diagnosis of juvenile idiopathic arthritis (JIA) is important to avoid long term complications. Elevated serum 14-3-3η levels improve the diagnostic sensitivity of rheumatoid factor (RF) and cyclic citrullinated peptide (CCP) antibody in adult rheumatoid arthritis (RA), and have been associated with more severe phenotype. We investigated the prevalence and clinical significance of serum 14-3-3η in different types of JIA.Methods: JIA patients (n=151) followed by the Pediatric Rheumatology Core at Children’s Hospital of Los Angeles were categorized into 5 groups: polyarticular JIA RF+ (PJIA RF+; n=39), PJIA RF- (n=39), psoriatic arthritis (PsA; n=19), enthesitis-related arthritis (ERA; n=18), and oligoarticular JIA (OJIA [control group]; n=36). RF, CCP antibody, and 14-3-3η were measured for all patients. 14-3-3η serum levels >0.2ng/mL were considered positive. Disease activity was assessed by the Juvenile Arthritis Disease Activity Score-71 (JADAS-71). Results: Elevated 14-3-3h levels were detected in 34/151 (23%) patients, and across all groups tested. Most patients with 14-3-3h had titers ≥4 times above the cutoff value. The majority (22, 65%) of 14-3-3h-positive patients were also positive for RF or CCP antibodies, 16 (47%) were positive for all 3, and 12 (35%) were single-positive for 14-3-3η. The highest prevalence of 14-3-3η was in PJIA RF+ patients (49%), followed by OJIA (22%). Positivity for 14-3-3h was not significantly associated with disease activity or age at diagnosis. Conclusion: Serum 14-3-3h can be detected in all forms of JIA tested but appears to be most common in PJIA RF+. 14-3-3h does not appear to correlate with disease activity in JIA.

183 EFFECTS OF LONG-TERM NUTRITIONAL RESTRICTION ON PREOVULATORY LUTEINIZING HORMONE SURGE, LEPTIN, AND LUTEAL PHASE IN LLAMAS (LAMA GLAMA)

2013 ◽  
Vol 25 (1) ◽  
pp. 240
Author(s):  
M. C. Norambuena ◽  
M. Silva ◽  
F. Urra ◽  
C. Ulloa-Leal ◽  
C. Letelier ◽  
...  
Keyword(s):  
Luteal Phase ◽  
Transvaginal Ultrasound ◽  
Plasma Concentrations ◽  
Maximum Diameter ◽  
Control Group ◽  
Blood Samples ◽  
Dependent Variables ◽  
Nutritional Restriction ◽  
Plasma Leptin

The aim of the study was to determine the effect of long-term energy restriction on preovulatory LH surge and luteal phase in llamas. Mature nonlactating, nonpregnant, female llamas (n = 16) were assigned randomly to the following groups: (1) llamas received Ballica sp. hay ad libitum and 300 g d–1 of commercial concentrate (control group, n = 8, BCS = 3.9) or (2) llamas were fed the same feed but the amount was progressively reduced from 70% to 40% of their maintenance energy requirement (MER) for a period of 87 days, until the final BCS was 2.5 (restricted group, n = 8). After 2 months and 21 days of 40% MER diet, ovarian follicular wave emergence was synchronized by transvaginal ultrasound-guided ablation of follicles ≥5 mm in diameter using a 17-ga needle attached to a 5-MHz convex-array transducer. Ten days after follicle ablation (Day 0), llamas were given an i.m. dose of 50 µg of gonadorelin acetate to induce ovulation. Frequent blood samples were taken every 30 min for 6 h and every 15 min for 1 h for plasma LH and leptin concentration measurements, respectively, immediately after treatment. Llamas were examined daily by transrectal ultrasonography for 20 days to record serial changes in the CL diameter. In addition, blood samples were taken every 2 days from Day 0 to 16 to determine plasma concentrations of leptin and progesterone. Discrete dependent variables were compared between groups by Student t-tests and continuous dependent variables were analysed by analyses of variance with repeated-measures. There was an effect of time on plasma LH concentration (P ≤ 0.001), but not an effect of group (P = 0.1) or interaction (P = 0.6) after gonadorelin acetate treatment. Plasma leptin concentration was lower in llamas from the restricted than that of the control group after gonadorelin treatment [effect of time, group (P ≤ 0.01), and interaction (P ≤ 0.01)]. The maximum diameter of the CL and plasma progesterone concentration were lower in the restricted than in the control group, and both variables were affected by group (P ≤ 0.01, P ≤ 0.02), and day (P ≤ 0.001, P ≤ 0.001, respectively). Plasma leptin concentration was lower in the restricted than that of the control group during the entire experiment. In conclusion, long-term nutritional restriction affected negatively CL diameter, progesterone secretion, and leptin plasma concentration in llamas. The differential response of leptin to gonadorelin acetate treatment could reflect a subfertlity-nutritional induced problem related with steroidogenesis, oocyte quality, ovulation, and early embryo development processes. Research supported by Postdoctoral Fondecyt N° 3110095.

scholarly journals Serum folate levels in children on long-term anti-seizure medication in a Nigerian tertiary center—Implications for practice and public health

2021 ◽  
Vol 104 (4) ◽  
pp. 003685042110576
Author(s):  
Adaobi I Bisi-Onyemaechi ◽  
Ugo N Chikani ◽  
Ndubuisi A Uwaezuoke ◽  
Ann E Aronu ◽  
Ngozi C Ojinnaka
Keyword(s):  
Folic Acid ◽  
Sodium Valproate ◽  
Serum Levels ◽  
Folic Acid Supplementation ◽  
Serum Folate ◽  
Control Group ◽  
Tertiary Center ◽  
Significant Difference ◽  
The Mean

Background: Epilepsy is the most common childhood neurological disorder in Nigeria. Treatment of epilepsy is long-term and sometimes lifelong with anti-seizure medications. There are conflicting reports on the effect of anti-seizure medications on serum folate. There is therefore a need to determine the effect of a commonly used anti-seizure medication's on serum folate levels of children. This would provide an evidence-based consideration for folic acid supplementation in children on anti-seizure medication as has been suggested by some studies. Study objectives: To determine whether serum folate levels were lower in children taking long-term carbamazepine or sodium valproate, compared to a control group. Methods: Serum folic acid levels were measured from well-nourished children between the ages of 1–17 years on carbamazepine and sodium valproate monotherapy and their age/sex-matched controls, using spectrophotometry. Results: The mean serum folate levels of patients on carbamazepine (43) and sodium valproate (22) were 0.032 mg/l ± 0.009 and 0.028 mg/l  ±  0.008, respectively. The mean folate levels of the controls were 0.046 mg/l  ±  0.03 ( p = 0 001). No statistically significant difference was observed between the serum folate levels of children on the two anti-seizure medications, that is, carbamazepine and valproate. Conclusion: The children on treatment with carbamazepine and sodium valproate for more than 6 months had statistically significantly lower serum levels of folic acid compared to the standard reference range and controls. The serum folate levels of children on carbamazepine were not statistically different from those on sodium valproate.

scholarly journals Long-term changes in the dentoalveolar system of rats after experimental intra-abdominal hypertension

2021 ◽  
Vol 12 (3) ◽  
pp. 38-46
Author(s):  
N. S. Morozova ◽  
A. A. Mamedov ◽  
D. Y. Lakomova ◽  
L. D. Maltseva ◽  
O. L. Morozova
Keyword(s):  
Blood Serum ◽  
Salivary Glands ◽  
Serum Levels ◽  
Control Group ◽  
Abdominal Pressure ◽  
Markers Of Inflammation ◽  
Abdominal Hypertension ◽  
Group 3 ◽  
Circulatory Disorders

Aim. To establish the effect of experimental intra-abdominal hypertension (IAH) on the teeth-jaw system of rats in the long-term period based on the study of pro-inflammatory cytokines and morphological analysis of the elements of the temporomandibular joint (TMJ) and salivary glands.Materials and methods. The experiment was carried out on 60 newborn rats; IAH was modelled by injecting collagen into the abdominal cavity to a predetermined level of intra-abdominal pressure. The rats were evenly (n = 20) divided into 3 groups: 1st - control; 2nd and 3rd - with light and severe IAH, respectively. Serum levels of IL-18, MCP-1, NGAL were determined by multiplex analysis after 10 and 120 days, VEGF-C - after 10 days - by ELISA. The morphological examination of the TMJ and salivary glands was performed using a Leica DM2000 microscope after 120 days.Results. After 10 days and 120 days, blood serum levels of NGAL, IL-18 and MCP-1 were statistically significantly increased in groups 2 and 3 compared to the control; the concentration of MCP-1 increased in proportion to the severity of the IAH with the maximum values in group 3. After 10 days, the level of VEGF was significantly increased in group 2 compared to the control group (p < 0.02). Inflammation of the TMJ was observed significantly more often in groups 2 and 3 than in the control group (p = 0.0002). In group 3, circulatory disorders and bone marrow degeneration of the TMJ, as well as inflammation, circulatory disorders and hyperplasia of the lymphoid tissue of the salivary glands were statistically significantly more often compared to groups 1 and 2.Conclusion. In the long term, experimental IAH showed an increase in the levels of markers of inflammation and hypoxia in the blood serum of rats; the severity of synovitis and sialadenitis grew with an increase in the level of intra-abdominal pressure; the maximum deviations in inflammation markers and morphological changes in the TMJ and salivary glands of rats were observed in the group with severe IAH.

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