scholarly journals Detection and discrimination of individual viruses by flow cytometry.

1979 ◽  
Vol 27 (1) ◽  
pp. 350-352 ◽  
Author(s):  
M Hercher ◽  
W Mueller ◽  
H M Shapiro
Keyword(s):  
Flow Cytometry ◽  
Quantitative Analysis ◽  
Light Scattering ◽  
Size Range ◽  
Flow Cytometer ◽  
Good Resolution ◽  
Observation Volume ◽  
Viral Particles ◽  
Scattering Measurements ◽  
Small Observation

A new flow cytometer with a very small observation volume has been developed to detect individual viruses with good resolution, and has been used to discriminate between two types of viral particles based on differences in their light scattering. Measurements of light scattering and fluorescence made with such an instrument can provide a basis for quantitative analysis and sorting of viruses and other particles in the micron and submicron size range.

Light-scattering detection within the difficult size range of protein particle measurement using flow cytometry

Nanoscale ◽  
2018 ◽  
Vol 10 (41) ◽  
pp. 19277-19285 ◽  
Author(s):  
Zhishang Hu ◽  
Chen Ye ◽  
Wei Mi ◽  
Yun Zhao ◽  
Can Quan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Light Scattering ◽  
Protein Aggregation ◽  
Size Range ◽  
Light Scattering Detection ◽  
Protein Particle ◽  
Particle Measurement

The phenomenon of protein aggregation is a prominent challenge that impacts biopharmaceutical development at every stage.

Quantitative analysis of gold and carbon nanoparticles in mammalian cells by flow cytometry light scattering

2017 ◽  
Vol 19 (2) ◽  
Author(s):  
Gang Zhou ◽  
Naicheng Liu ◽  
Zhenheng Wang ◽  
Tongguo Shi ◽  
Jingjing Gan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Quantitative Analysis ◽  
Light Scattering ◽  
Mammalian Cells ◽  
Carbon Nanoparticles

Scattering Rings in Birefringent Porous Silicon

2003 ◽  
Vol 762 ◽  
Author(s):  
Claudio J. Oton ◽  
Zeno Gaburro ◽  
Mher Ghulinyan ◽  
Nicola Daldosso ◽  
Lucio Pancheri ◽  
...  
Keyword(s):  
Dielectric Constant ◽  
Light Scattering ◽  
Porous Silicon ◽  
Direct Measurement ◽  
Optical Anisotropy ◽  
Oblique Incidence ◽  
Laser Light ◽  
Anisotropic Scattering ◽  
Scattering Measurements

AbstractWe report the observation of strongly anisotropic scattering of laser light at oblique incidence on (100)-oriented porous silicon layers. We performed angle-resolved light scattering measurements and three concentric rings were observed. Modeling porous silicon by means of nanometric columnar air pores and an effective anisotropic uniaxial dielectric constant explains the observed phenomenon, and besides, the observation of the angle aperture of these rings allows a direct measurement of relative birefringence. We finally study the changes of optical anisotropy after different modifications of the structure.

scholarly journals Experimental Results on Scattering by Irregular and Meteoritic Dust Particles Related to Photopolarimetry of Zodiacal Light and Comets

1985 ◽  
Vol 85 ◽  
pp. 223-226
Author(s):  
K. Weiss-Wrana ◽  
R.H. Giese ◽  
R.H. Zerull
Keyword(s):  
Light Scattering ◽  
Size Range ◽  
Experimental Results ◽  
Dust Particles ◽  
Dust Grains ◽  
Scattering Function ◽  
Negative Polarization ◽  
Zodiacal Light ◽  
Cometary Dust ◽  
Characteristic Features

AbstractThe investigations of light scattering by larger meteoritic and terrestrial single grains (size range 20 μm to 120 μm ) demonstrate that the scattering properties of irregularly shaped dark opaque particles with very rough surfaces resemble the characteristic features of the empirical scattering function as derived from measurements of the zodiacal light. Purely transparent or translucent irregularly shaped particles show a quite different scattering behaviour. Furthermore irregular and multicomponent fluffy particles in the size range of a few microns were modelled by microwave analog measurements in order to explain positive and negative polarization of the light scattered by cometary dust grains.

scholarly journals Quantitative analysis of phosphatidylcholine molecular species using HPLC and light scattering detection

1998 ◽  
Vol 39 (2) ◽  
pp. 344-353 ◽  
Author(s):  
Jos F.H.M. Brouwers ◽  
Barend M. Gadella ◽  
Lambert M.G. van Golde ◽  
Aloysius G.M. Tielens
Keyword(s):  
Quantitative Analysis ◽  
Light Scattering ◽  
Molecular Species ◽  
Light Scattering Detection

scholarly journals Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1320
Author(s):  
Kristýna Pekárková ◽  
Jakub Soukup ◽  
Marie Kostelanská ◽  
Jan Širc ◽  
Zbyněk Straňák ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Extracellular Vesicles ◽  
Correlation Coefficients ◽  
Flow Cytometer ◽  
Liquid Biopsies ◽  
Physiological Conditions ◽  
Flow Cytometric ◽  
Term Births ◽  
And Control

Extracellular vesicles (EVs) from liquid biopsies are extensively analyzed by flow cytometry, a technology that is continuously evolving. Thresholding utilizing a violet 405 nm laser side scatter (VSSC) has recently been implemented. Here, we collected set of large EV (lEV) samples from cord blood, which we analyzed using a standard flow cytometer improved via a 405 nm laser side scatter. Samples were analyzed using two distinct thresholding methods—one based on VSSC, and one based on VSSC combined with fluorescence thresholding on stained phosphatidylserine. Through these thresholding methods, we compared lEVs from pre-term births and control cord blood. Double-labeled lEVs with platelet CD36+/CD41+, activated platelet CD41+/CD62P+ and endothelial CD31+/CD105+ antibodies were used. Apart from comparing the two groups together, we also correlated measured lEVs with the thresholding methods. We also correlated the results of this study with data analyzed in our previous study in which we used a conventional 488 nm laser SSC. We did not find any difference between the two cord blood groups. However, we found highly concurrent data via our correlation of the thresholding methods, with correlation coefficients ranging from 0.80 to 0.96 even though the numbers of detected lEVs differed between thresholding methods. In conclusion, our approaches to thresholding provided concurrent data and it seems that improving the cytometer with the use of a VSSC increases its sensitivity, despite not being particularly critical to the validity of flow cytometric studies that compare pathological and physiological conditions in liquid biopsies.

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