Forward and 90-Degree Light Scattering Measurements in Optofluidic Flow Cytometer

2010 ◽  
Author(s):  
Jessica Godin ◽  
Yu-Hwa Lo
Keyword(s):  
Light Scattering ◽  
Flow Cytometer ◽  
Scattering Measurements

scholarly journals Detection and discrimination of individual viruses by flow cytometry.

1979 ◽  
Vol 27 (1) ◽  
pp. 350-352 ◽  
Author(s):  
M Hercher ◽  
W Mueller ◽  
H M Shapiro
Keyword(s):  
Flow Cytometry ◽  
Quantitative Analysis ◽  
Light Scattering ◽  
Size Range ◽  
Flow Cytometer ◽  
Good Resolution ◽  
Observation Volume ◽  
Viral Particles ◽  
Scattering Measurements ◽  
Small Observation

A new flow cytometer with a very small observation volume has been developed to detect individual viruses with good resolution, and has been used to discriminate between two types of viral particles based on differences in their light scattering. Measurements of light scattering and fluorescence made with such an instrument can provide a basis for quantitative analysis and sorting of viruses and other particles in the micron and submicron size range.

Scattering Rings in Birefringent Porous Silicon

2003 ◽  
Vol 762 ◽  
Author(s):  
Claudio J. Oton ◽  
Zeno Gaburro ◽  
Mher Ghulinyan ◽  
Nicola Daldosso ◽  
Lucio Pancheri ◽  
...  
Keyword(s):  
Dielectric Constant ◽  
Light Scattering ◽  
Porous Silicon ◽  
Direct Measurement ◽  
Optical Anisotropy ◽  
Oblique Incidence ◽  
Laser Light ◽  
Anisotropic Scattering ◽  
Scattering Measurements

AbstractWe report the observation of strongly anisotropic scattering of laser light at oblique incidence on (100)-oriented porous silicon layers. We performed angle-resolved light scattering measurements and three concentric rings were observed. Modeling porous silicon by means of nanometric columnar air pores and an effective anisotropic uniaxial dielectric constant explains the observed phenomenon, and besides, the observation of the angle aperture of these rings allows a direct measurement of relative birefringence. We finally study the changes of optical anisotropy after different modifications of the structure.

A STUDY OF A WATER-SOLUBLE COMPLEX OF PRODIGIOSIN PRODUCED BY A STRAIN OF SERRATIA MARCESCENS

1962 ◽  
Vol 40 (1) ◽  
pp. 1019-1024 ◽  
Author(s):  
Seiichi Yoshida
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Serratia Marcescens ◽  
Average Molecular Weight ◽  
Electrophoretic Analysis ◽  
Water Soluble ◽  
Soluble Complex ◽  
Ph Values ◽  
Scattering Measurements ◽  
Water Soluble Complex

A water-soluble pigment excreted from Serratia marcescens has been purified by precipitation with ammonium sulphate, dialysis, and ultracentrifugation at different pH values. The purified pigment showed a single band in the ultracentrifuge and by electrophoretic analysis at several pH values. An average molecular weight of 5 × 106 was calculated from light-scattering measurements. This pigment is composed of carbohydrate and protein combined with prodigiosin, and several properties of the complex are described.

Light scattering measurements on microemulsions: Estimation of droplet sizes

2006 ◽  
Vol 312 (1-2) ◽  
pp. 187-195 ◽  
Author(s):  
C. Goddeeris ◽  
F. Cuppo ◽  
H. Reynaers ◽  
W.G. Bouwman ◽  
G. Van den Mooter
Keyword(s):  
Light Scattering ◽  
Scattering Measurements ◽  
Droplet Sizes

Light Scattering and Flow Dichroism Studies on DNA After the Photoreaction with Psoralen

1972 ◽  
Vol 27 (2) ◽  
pp. 196-200 ◽  
Author(s):  
S. Marciani ◽  
M. Terbojevic ◽  
F. Dall’Acqua
Keyword(s):  
Heat Treatment ◽  
Molecular Weight ◽  
Light Scattering ◽  
Double Helix ◽  
Heat Denaturation ◽  
Scattering Measurements ◽  
Helix Structure ◽  
Double Helix Structure

Light scattering measurements performed on DNA after irradiation in the presence of psoralen clearly show that inter strand cross linkings are present in the macromolecule. In fact after heat denaturation and successive cooling irradiated macromolecule shows a molecular weight practically unchanged while a DNA sample after the same treatment shows a molecular weight half of the intact native DNA. Also the general conformation of irradiated DNA undergoes practically to no modifications after the same heat treatment while native DNA shows itself to have been strongly modified. Moreover, on the basis of flow dichroism determinations, DNA cross-linked by psoralen after heat denaturation showed to be able to restore its ordered double helix structure, during the successive cooling.

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