scholarly journals Reproductive tract peroxidases as endproducts of estrogen-specific gene expression.

1979 ◽  
Vol 27 (10) ◽  
pp. 1363-1364 ◽  
Author(s):  
W A Anderson ◽  
C Burnett
Keyword(s):  
Gene Expression ◽  
Reproductive Tract ◽  
Mammary Glands ◽  
Receptor Protein ◽  
Specific Gene ◽  
Specific Marker ◽  
Essential Requirement ◽  
Cell Biol ◽  
Marker Proteins ◽  
Specific Mrna

Estrogen is an essential requirement for the postpubertal trophic development and maintenance of the differentiated state of the oviduct, uterus, cervix, vagina and mammary glands of mammals. Estrogen, apparently functioning through its specific cytoplasmic receptor protein via a multistep interaction pathway induces gene expression of specific biochemical events leading to growth and differentiation of target tissues (Jensen et al., Proc Natl Acad Sci, 59:632, 1968; Gorski et al., Recent Prog Horm Res 24:45, 1968). One biochemical expression of the estrogen gene is the synthesis of specific mRNA transcripts for certain specific marker proteins, including ovalbumin, lysozyme and ovomucoid in the chick oviduct (O'Malley and McGuire, Proc Natl Acad Sci 60:1527, 1968; Palmiter and Schimke, J Biol Chem 248:1502, 1973), tubulin in the mammalian oviduct (Brenner and Anderson, Handbook of Physiology 7(2):123, 1973; Brenner et al., Endocrinology 95:1094, 1974) and peroxidase (EC 1,11.1.7) in the rodent uterus (Brockelmann and Fawcett, Biol Reprod 1:59, 1969; Churg and Anderson, J Cell Biol 62:449, 1974; Anderson et al., J Cell Biol 64:668, 1975).

scholarly journals Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wiruntita Chankeaw ◽  
Sandra Lignier ◽  
Christophe Richard ◽  
Theodoros Ntallaris ◽  
Mariam Raliou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Localization ◽  
Expression Profiles ◽  
Cell Types ◽  
Molecular Signature ◽  
Receptor Protein ◽  
Specific Gene ◽  
Specific Cell ◽  
Biological Processes ◽  
Endometrial Cells

Abstract Background A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. Results In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. Conclusion The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms.

scholarly journals Differential rescue of visceral and cardiac defects inDrosophilaby vertebratetinman-related genes

1998 ◽  
Vol 95 (16) ◽  
pp. 9366-9371 ◽  
Author(s):  
Maijon Park ◽  
Carol Lewis ◽  
David Turbay ◽  
Amy Chung ◽  
Jau-Nian Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heart Development ◽  
Molecular Mechanisms ◽  
Marker Gene ◽  
Homeobox Gene ◽  
Specific Gene ◽  
Specific Marker ◽  
Cardiac Progenitors ◽  
Mesoderm Development ◽  
Visceral Mesoderm

tinman, a mesodermal NK2-type homeobox gene, is absolutely required for the subdivision of the earlyDrosophilamesoderm and for the formation of the heart as well as the visceral muscle primordia. Several vertebrate relatives oftinman, many of which are predominately expressed in the very early cardiac progenitors (and pharyngeal endoderm), also seem to promote heart development. Here, we show that most of these vertebratetinman-related genes can readily substitute forDrosophila tinmanfunction in promoting visceral mesoderm-specific marker gene expression, but much less in promoting cardiac-specific gene expression indicative of heart development. In addition, another mesodermal NK2-type gene fromDrosophila,bagpipe, which is normally only needed for visceral mesoderm but not heart development, cannot substitute fortinmanat all. These data indicate that the functional equivalence of thetinman-related subclass of NK2-type genes (in activating markers of visceral mesoderm development inDrosophila) is specific to this subclass and distinct from other homeobox genes. Despite the apparent overall conservation of heart development between vertebrates and invertebrates, the differential rescue of visceral mesoderm versus heart development suggests that some of the molecular mechanisms of organ formation may have diverged during evolution.

scholarly journals Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
You Ning ◽  
Jian-Hua Huang ◽  
Shi-Jin Xia ◽  
Qin Bian ◽  
Yang Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Dna Microarray ◽  
Specific Gene ◽  
Specific Marker ◽  
Dependent Manner ◽  
Adult Neural Stem Cells ◽  
Medicine Research ◽  
Novel Treatments

Adult neural stem cells (NSCs) persist throughout life to replace mature cells that are lost during turnover, disease, or injury. The investigation of NSC creates novel treatments for central nervous system (CNS) injuries and neurodegenerative disorders. The plasticity and reparative potential of NSC are regulated by different factors, which are critical for neurological regenerative medicine research. We investigated the effects of Psoralen, which is the mature fruit ofPsoralea corylifolia L., on NSC behaviors and the underlying mechanisms. The self-renewal and proliferation of NSC were examined. We detected neuron- and/or astrocyte-specific markers using immunofluorescence and Western blotting, which could evaluate NSC differentiation. Psoralen treatment significantly inhibited neurosphere formation in a dose-dependent manner. Psoralen treatment increased the expression of the astrocyte-specific marker but decreased neuron-specific marker expression. These results suggested that Psoralen was a differentiation inducer in astrocyte. Differential gene expression following Psoralen treatment was screened using DNA microarray and confirmed by quantitative real-time PCR. Our microarray study demonstrated that Psoralen could effectively regulate the specific gene expression profile of NSC. The genes involved in the classification of cellular differentiation, proliferation, and metabolism, the transcription factors belonging to Ets family, and the hedgehog pathway may be closely related to the regulation.

scholarly journals Impacts of Macronutrients on Gene Expression: Recent Evidence to Understand Productive and Reproductive Performance of Livestock

2018 ◽  
Vol 6 (2) ◽  
pp. 203 ◽  
Author(s):  
Md. Mahmodul Hasan Sohel ◽  
Yusuf Konca ◽  
Mehmet Ulas Cinar
Keyword(s):  
Gene Expression ◽  
Reproductive Performance ◽  
Quantitative Measurement ◽  
Molecular Level ◽  
Specific Gene ◽  
Qpcr Array ◽  
Use Of Knowledge ◽  
The Impact ◽  
Generation Sequencing ◽  
Specific Mrna

In order to identify the effects of nutrients on gene expression and to assess the interactions between genes and nutrition by means of various cutting-edge technologies, the interdisciplinary branch ‘Nutrigenomics’ was created. Therefore, nutrigenomics corresponds to the use of knowledge and techniques of nutrition, genomics, transcriptomics, proteomics, epigenomics, and metabolomics to seek and explain the cross-talk between nutrition and genes in molecular level. Macronutrients are important dietary signals that control metabolic programming of cells and have important roles in maintaining cellular homeostasis by influencing specific gene expression. Recent advancements in molecular genetics studies, for instance, use of next-generation sequencing, microarray and qPCR array to investigate the expression of transcripts, genes, and miRNAs, has a crucial impact on understanding and quantitative measurement of the impact of dietary macronutrients on gene function. This review will shade a light on the interactions and mechanisms how the dietary source of macronutrients changes the expression of specific mRNA and miRNA. Furthermore, it will highlight the exciting recent findings in relation to animal performance characteristics which eventually help us to identify a dietary target to improve animal production.

scholarly journals Spatial transcriptomics of C. elegans males and hermaphrodites identifies novel fertility genes

2018 ◽  
Author(s):  
Annabel Ebbing ◽  
Abel Vertesy ◽  
Marco Betist ◽  
Bastiaan Spanjaard ◽  
Jan Philipp Junker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reproductive Tract ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Specific Gene ◽  
Comprehensive Overview ◽  
Tissue Specific ◽  
C Elegans ◽  
Differential Gene ◽  
Fertility Genes

SummaryTo advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used RNA tomography to generate the first high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region and tissue-specific genes. Moreover, by combining pattern-based analysis with differential gene expression analysis, we have found extensive sex-specific gene expression differences in the germline and sperm. We have also identified genes that are specifically expressed in the male reproductive tract, including a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying novel tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, which opens up new possibilities for transcriptome-wide comparisons of gene expression patterns.

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