Nitrite Ion Fluxes in Human Erythocytes.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3731-3731
Author(s):  
Chiara d’Agostino ◽  
Andre Dejam ◽  
Mildred Pelletier ◽  
Nathawut Sibmooh ◽  
Fabiola Rizzatti ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Whole Blood ◽  
Half Life ◽  
Rapid Loss ◽  
Nitrite Ion ◽  
Human Forearm ◽  
Nitrite Uptake ◽  
Rapid Modulation ◽  
Forearm Circulation ◽  
Free Plasma

Abstract Our recent studies suggest that nitrite ions serve as a storage pool of nitric oxide (NO•) bioactivity, with reduction of nitrite to NO• within red blood cells (RBC), thus potentially explaining endocrine effects of NO•. We have investigated nitrite uptake and decay in human RBC using recently described methodologies based on assaying after stabilization of nitrite to reaction with hemoglobin by treatment with ferricyanide and quantificating by reductive chemiluminescence. Uptake of nitrite added to whole blood is very rapid (sec) and independent of temperature (from 4°C to 37°C) and state of oxygenation of the cells, or treatment with carbon monoxide. We obtain intracellular values as high as 711 ± 45.44 nM/l with an extracellular value of 10,935.089 ± 897.370 nM/l. Decay of nitrite levels within the RBC is, however, strongly temperature and ligand dependent. At 37°C only 22% of the original maximal value remains at 30 minutes (half life: 5.24 min), while there is no apparent decay at 4°C. Carbon monoxide also prevents nitrite destruction. If the nitrite-containing RBC are suspended in nitrite-free plasma, decay of nitrite levels is faster (half life 2.1min) suggesting export of nitrite as well as reaction with hemoglobin. We have also observed whole blood and RBC artery-to-vein gradients of nitrite levels in human forearm circulation suggesting rapid loss during RBC transit of the microcirculation. These results suggest rapid fluxes of nitrite ions into and out of RBC, as well as controlled intra-erythrocytic metabolism, which could contribute to local, rapid modulation of blood flow by mechanisms involving nitrite activation to NO• by intra-erythrocytic hemoglobin.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

Polymer–protein hybrid scaffolds as carriers for CORM-3: platforms for the delivery of carbon monoxide (CO)

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92975-92980 ◽  
Author(s):  
Diep Nguyen ◽  
Susan Oliver ◽  
Nik Nik M. Adnan ◽  
Cristan Herbert ◽  
Cyrille Boyer
Keyword(s):  
Carbon Monoxide ◽  
Half Life ◽  
Protein Conjugate ◽  
Hybrid Scaffolds

The carbon monoxide releasing molecule, CORM-3, was grafted onto a polymer–protein conjugate thereby improving its half-life and release characteristics.

scholarly journals In-line whole blood fractionation for Raman analysis of blood plasma

The Analyst ◽  
2019 ◽  
Vol 144 (2) ◽  
pp. 602-610 ◽  
Author(s):  
Moritz Matthiae ◽  
Xiaolong Zhu ◽  
Rodolphe Marie ◽  
Anders Kristensen
Keyword(s):  
Blood Flow ◽  
Blood Plasma ◽  
Whole Blood ◽  
Raman Analysis ◽  
Free Plasma

Raman studies of dynamically expanded cell-free plasma domains in microfluidic blood flow.

Rate of loss of radioiron from mouse and man

1960 ◽  
Vol 198 (4) ◽  
pp. 784-786 ◽  
Author(s):  
John D. Bonnet ◽  
Alan L. Orvis ◽  
Albert B. Hagedorn ◽  
Charles A. Owen
Keyword(s):  
Life Span ◽  
Half Life ◽  
Whole Body ◽  
Entire Body ◽  
Rapid Loss ◽  
Male And Female ◽  
Female Mice ◽  
Steady Rate ◽  
Normal Man ◽  
The Difference

Forty-two male and female mice, 8 weeks old, were given radioiron (Fe59) in doses of 0.006–0.1 µc, containing 0.013–0.17 µg of iron, by intraperitoneal or intravenous routes. Assays of the radioactivity of the whole body revealed an initial rapid loss of Fe59 (15–20%) lasting about 6 days. Thereafter the Fe59 left the mice at a steady rate of 0.39%/day (half-life 180 days). One 34-year-old normal man was given 10.6 µc of Fe59, containing 8.2 µg of iron, intravenously. Based on counts from the entire body, the biologic rate of loss of the Fe59 was about 0.14%/day (half-life 500 days), and there was little or no initial loss such as occurred in the mouse. The Fe59 in the circulating erythrocytes was essentially unchanged for the first 3 months. It then fell to a new level of about 90% of the previous one; the mid-point of the fall was about 120 days after the administration of the radioiron. The difference in the rates of loss of radioiron from mice and man seems to be related primarily to the life span of the circulating red cells.

Determination of carboxyhemoglobin half-life in patients with carbon monoxide toxicity treated with high flow nasal cannula oxygen therapy

2019 ◽  
Vol 57 (7) ◽  
pp. 617-623 ◽  
Author(s):  
Ibrahim Ulas Ozturan ◽  
Elif Yaka ◽  
Selim Suner ◽  
Asim Enes Ozbek ◽  
Cansu Alyesil ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Half Life ◽  
Oxygen Therapy ◽  
High Flow ◽  
Nasal Cannula ◽  
High Flow Nasal Cannula

Automated Monitoring of Platelet Aggregation in Multiple Samples of whole Blood

1975 ◽  
Author(s):  
N. Clarke ◽  
V. McCabe
Keyword(s):  
Whole Blood ◽  
Light Emitting Diode ◽  
Direct Reading ◽  
Light Emitting ◽  
Printed Circuit ◽  
Platelet Concentration ◽  
Light Effects ◽  
Multiple Samples ◽  
Free Plasma ◽  
Selection Of

Whole blood may be first agitated in a circular transparent rotor chamber, and then rotated continuously to produce plasma/red cell separation for monitoring. The rotor is driven by a low inertia, printed circuit d.c. motor (108 Watt) which is automatically controlled. The pattern of rotation is determined by a signal generator which provides various waveforms at adjustable frequencies and also at fixed speeds.By segmenting the rotor into multiple cuvettes (Vol = 0.4 ml) it is possible to monitor the platelet concentration, after agitation, as each cuvette spins through the stationary optical system. This consists of a light-emitting diode (peak: 900 nm), pulsed at 10 kHz in order to eliminate ambient light effects, and a photodetector with a built-in amplifier, fed to an amplifying circuit which eliminates other signals. An electronic circuit permits selection of the signal from each cuvette on a direct reading meter. Cuvette No. 1 is identified by a photocell-detector trigger.Platelet-free plasma is about zero O. D. in the infra red with respect to a water blank, and with attention to specific criteria such as optical geometry and light path, platelet concentration may be accurately determined.

Local tissue factor pathway inhibitor release in the human forearm

2003 ◽  
Vol 89 (03) ◽  
pp. 438-445 ◽  
Author(s):  
Jacobus Burggraaf ◽  
Rik Schoemaker ◽  
Adam Cohen ◽  
Cornelis Kluft ◽  
Stanley Chia ◽  
...  
Keyword(s):  
Substance P ◽  
Sodium Nitroprusside ◽  
Regional Differences ◽  
Systemic Circulation ◽  
Unfractioned Heparin ◽  
Human Forearm ◽  
Tissue Factor Pathway ◽  
Forearm Circulation ◽  
Dose Dependent Increase ◽  
Dose Dependent

SummaryNineteen healthy men received unilateral brachial artery infusions of either unfractioned heparin (0.3-100 IU/min), saline or the endothelium-dependent vasodilators substance P (2-8 pmol/min) and bradykinin (100-1000 pmol/min), and the endothelium-independent vasodilator sodium nitroprusside (2-8 µg/min). Heparin caused a dose-dependent increase in plasma TFPI concentrations in both arms (ANOVA, p <0.0001). Estimated net forearm TFPI release was 7 ± 16, 29 ± 20 and 138 ± 72 ng/100 mL tissue/min during 10, 30 and 100 IU/min of heparin respectively (ANOVA, p <0.0001). Compared to the systemic circulation, the forearm sensitivity to heparin induced TFPI release was 3.6-fold lower (166 ± 67 ng/IU vs. 596 ± 252 ng/IU: t-test, p = 0.004). Substance P, bradykinin and sodium nitroprusside all caused substantial dose-dependent increases in blood flow (ANOVA, p <0.001 for all) without affecting plasma TFPI concentrations. There are important regional differences in endothelial TFPI release, with the forearm circulation being relatively insensitive to heparin.

Measurement of blood O2 and CO2 concentrations using PO2 and PCO2 electrodes

1978 ◽  
Vol 44 (5) ◽  
pp. 818-820 ◽  
Author(s):  
A. L. Harabin ◽  
L. E. Farhi
Keyword(s):  
Carbon Monoxide ◽  
Blood Volume ◽  
Whole Blood ◽  
Co2 Concentration ◽  
Saturated Solution ◽  
Additional Step ◽  
Co2 Concentrations ◽  
O2 Concentration ◽  
Co2 Electrode ◽  
Small Blood Volume

Accurate dilution of a small blood volume with a carbon monoxide-saturated solution allows measurement of the whole blood O2 concentration as an increase in O2 tension in the solution. We have improved the method by simplifying both equipment and procedure. We also suggest an additional step in which the mixture is acidified, thereby allowing the measurement of CO2 concentration in the same solution with a CO2 electrode. The accuracy of both the O2 and CO2 determinations compares favorably with that obtained with other micromethods.

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