Detection of ZAP70 Negative B-CLL Patients Using the Beckman Coulters Positional Parameter Technology and RPD (Research Population Data) Analysis.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4934-4934
Author(s):  
Varda Deutsch ◽  
Yair Herishanu ◽  
Aaron Polliack ◽  
Elizabeth Naparstek ◽  
Dina Meyuhas ◽  
...  
Keyword(s):  
Protein Tyrosine Kinase ◽  
Population Data ◽  
Good Prognosis ◽  
Receiver Operating Curve ◽  
Control Group ◽  
Hematological Disorders ◽  
Volume Conductivity ◽  
Aggressive Disease ◽  
Positional Parameter ◽  
Receiver Operating Curve Analysis

Abstract B-CLL, the most common type of leukemia in adults, is heterogeneous with a varying clinical course ranging from indolent to aggressive disease. CLL patients with mutated Ig V(H) genes have a good prognosis while those with non-mutated Ig V(H) status and expression of the ZAP70 protein tyrosine kinase generally have aggressive disease and shorter survival. Testing for IgV(H) mutations is not routinely performed due to cost and ZAP70 testing is still technically difficult. As these tests remain generally inappropriate for B-CLL patient screening, we tested the capacity of the new Beckmann Coulter LH750 blood analyzers to provide additional morphometric information on the CLL cell population. These analyzers use a highly informative positional parameter technology - VCS (Volume, Conductivity, Scatter) technology, and automated RPD analysis which provide the Mean and Standard Deviation of Volume, Conductivity and Scatter (VCS) for each of the main WBC types (neutrophils, lymphocytes, monocytes and eosinophils), thereby enabling the detection of abnormal WBC populations associated with various hematological and non-hematological disorders. We attempted to determine if the RPD generated could differentiate between the ZAP70pos and ZAP70neg B-CLL subgroups as determined by high resolution quantitative ZAP70 flow cytometry analysis (1). This study included 30 newly diagnosed untreated CLL patients. CBC was performed on all samples using automated leukocyte differentials in the Beckmann-Coulter LH750. Statistical analysis was performed with Medcalc® 8.1.1.0. Results show that there was no correlation between the WBC number, ZAP70pos expression (found in 59% of the cases) and lymphocyte volume, scatter, or conductivity or SD of these parameters. However, differences in the automated morphology of the Zap70neg compared with Zap70pos CLL lymphocytes was detected. When compared to normal lymphocyte volume (85.8±14.5), the Lymphocyte Mean Volume (MLYV) was lower in the ZAP70neg samples (77.7±17.6) (p<0.05), while the ZAP70pos samples had increased volumes (87.5±15.8), similar to values reported in other myeloproliferative states. The conductivity of the ZAP70neg cells was reduced (110±15.0) below normal (117±11.5) (p=0.038), while the ZAP70pos cells had conductivity similar to the control group. Higher levels of lymphocyte apoptosis were clearly observed in the ZAP70neg samples by VCS. When using ROC (receiver operating curve analysis) to detect the potential ZAP70neg cases, the cut off proposed is MLYV < 76 with a sensitivity of 69.2% and a specificity of 70% AUC 0,650. Herein we propose an entirely new approach which may provide a novel diagnostic screening approach to enable identification of CLL patients who do not require further complex testing. This data is derived directly from the CBC of the Beckman-Coulter LH750 analyzers at no extra cost. As it will be necessary to analyze many more cases from different laboratories, an international working group has been formed in an attempt to confirm these early observations.

scholarly journals Evaluation of endothelial dysfunction in COVID-19 with flow-mediated dilatation

2021 ◽  
Author(s):  
Aslı Kurtar Mansıroğlu ◽  
Hande SEYMEN ◽  
İsa Sincer ◽  
Yilmaz Gunes
Keyword(s):  
Endothelial Dysfunction ◽  
Curve Analysis ◽  
Receiver Operating Curve ◽  
Control Group ◽  
Receiver Operating Curve Analysis ◽  
Significant Difference ◽  
Flow Mediated Dilatation ◽  
Echocardiographic Parameters ◽  
Forward Stepwise ◽  
Spearman’S Correlation

Aim: It is a well-known fact that inflammation plays a crucial role in many diseases including COVID-19. Using flow-mediated dilatation (FMD), we aimed to compare the effects of inflammation on endothelial dysfunction in patients with COVID-19 and the control group. Materials and Methods: The present study was conducted on a total of 161 participants, of whom 80 were diagnosed with COVID-19 within the last 6 months (comprising 48 women and 32 men with a mean age of 32.10 ± 5.87 years) and 81 were healthy controls (comprising 45 women and 36 men with a mean age of 30.51 ± 7.33 years). We analyzed the findings of transthoracic echocardiography and FMD in all participants. Results: Except for FMD, there was no statistically significant difference in echocardiographic parameters. (9.52 ± 5.98 vs. 10.53 ± 6.31, p=0.010). In multivariate analysis with the forward stepwise model, FMD was significantly different in the control group compared to the COVID group (1.086 (1.026 - 1.149), p=0.04). Spearman’s correlation test indicated that FMD (r=0.27, p=0.006) had a significantly positive correlation with the presence of COVID. A receiver operating curve analysis revealed that an FMD value of <10.62% was capable of predicting the presence of COVID with a sensitivity and specificity of 64% and 59%, respectively (AUC=0.625, 95% CI, 0.538 - 0.711). Conclusion: The value of FMD decreased significantly in COVID-19 patients compared to the healthy subjects, which may be an early marker for COVID-19 induced endothelial dysfunction. KEYWORDS: COVID-19, endothelial dysfunction, flow-mediated dilatation (FMD

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

scholarly journals Expression of Transcript Variants of PTGS1 and PTGS2 Genes among Patients with Chronic Rhinosinusitis with Nasal Polyps

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 135
Author(s):  
Wioletta Pietruszewska ◽  
Wojciech Fendler ◽  
Marta Podwysocka ◽  
Adam J. Białas ◽  
Piotr Kuna ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Direct Sequencing ◽  
Adult Patients ◽  
Control Group ◽  
Reliable Test ◽  
Aggressive Disease ◽  
Transcript Variants

To date, there has been no reliable test to identify unfavorable course of Chronic Rhinosinusitis with Nasal Polyps (CRSwNP), especially in aspirin intolerant patients. The research aimed to analyze the expression of transcript variants of PTGS1 and PTGS2 genes in the pathobiology of the disease. The study was performed on 409 adult patients: 206 CRSwNP patients including 44 (21.36%) aspirin intolerant patients and 203 healthy volunteers in the control group. Transcript variants of the PTGS1 and PTGS2 genes named as follows: COX1.1 for NM_000962, COX1.2 for NM_080591, COX1.3 for NM_001271165.1, COX1.4 for NM_001271368.1, COX1.5 for NM_001271166.1, COX2.1 for NM_000963.3, COX2.2 for AY_151286 and COX2.3 for BQ_722004 were confirmed using direct sequencing and quantified using targeted qPCR. The coexistence of all examined transcript variants in the study and the control group and significant differences between both were found. In aspirin sensitive patients, the levels of COX1.2, COX1.3, COX1.4 and COX1.5 isoforms were higher compared to aspirin-tolerant patients. The severity of symptoms was bigger in patients with higher expressions of variants: COX1.1 (R with dCt = −0.134; p = 0.0490), COX1.3 (R = −0.1429; p = 0.0400) and COX1.5 (Rs = −0.1499; p = 0.032). The expression of COX1.1 (Rs = −0.098; p = 0.049) and COX1.5 (Rs = −0.141; p = 0.043) isoforms increased with polyposis advancement in endoscopy. With the CT extent of sinuses opacification, COX1.1 isoform also significantly increased (Rs = −0.163; p = 0.020). The isoforms COX1.3, COX1.4, COX1.5 and COX2.1 may promote milder CRSwNP course. On the contrary, the variants COX1.1, COX1.2 and COX2.2 may be involved in a more aggressive disease.

Evaluation of the Clinical Role of Strain Elastography in Patients Diagnosed With Congenital Torticollis

2021 ◽  
pp. 875647932098324
Author(s):  
Elif Özyazici Özkan ◽  
Mehmet Burak Ozkan ◽  
İshak Abdurrahman İsik
Keyword(s):  
Area Under The Curve ◽  
Muscle Group ◽  
Receiver Operating Curve ◽  
Control Group ◽  
Clinical Role ◽  
Congenital Muscular Torticollis ◽  
Cutoff Value ◽  
Strain Elastography ◽  
Specificity And Sensitivity ◽  
Muscular Torticollis

Objective: The objective of this study was to determine the elasticity of sternocleidomastoid muscle (SCM) in patients with congenital muscular torticollis (CMT). Methods: In all, 41 patients and 22 controls were included in the study, and the elasticity of the patients’ SCM was measured. Echogenicity, thickness, and strain values of the SCM were also obtained. Results: The thickness and strain values of the SCM were higher in the patient group than in the control group ( P = .02 and P = .15). For median values, there was no difference in echogenicity and strain. In the strain elastography evaluation of the receiver operating curve (ROC) for muscle echogenicity in the isoechoic muscle group, the specificity and sensitivity were determined to be 100% and 22%, respectively, for the area under the curve (AOC) value of 0.558 (95% confidence interval [CI], 0.424–0.6686), and the cutoff value was <1.4. In the hyperechoic muscle group, the ROC for AUC values was found to be 0.542 (95% CI, 0.411–0.6686), and the cutoff value was >1.4 with 100% sensitivity and 20.75 specificity. Conclusions: The strain elastography technique can be used in the diagnosis of CMT.

Ultrasonic Adaptive Sound Speed Estimation for the Diagnosis and Quantification of Hepatic Steatosis: A Pilot Study

2018 ◽  
Vol 40 (06) ◽  
pp. 722-733 ◽  
Author(s):  
Marco Dioguardi Burgio ◽  
Marion Imbault ◽  
Maxime Ronot ◽  
Alex Faccinetto ◽  
Bernard E. Van Beers ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Sound Speed ◽  
Validation Cohort ◽  
Liver Steatosis ◽  
Receiver Operating Curve ◽  
Coefficient Of Determination ◽  
Proton Density ◽  
Speed Estimation ◽  
Training Cohort ◽  
Receiver Operating Curve Analysis

Abstract Purpose To evaluate the ability of a new ultrasound (US) method based on sound speed estimation (SSE) with respect to the detection, quantification, and grading of hepatic steatosis using magnetic resonance (MR) proton density fat fraction (PDFF) as the reference standard and to calculate one US fat index based on the patient’s SSE. Materials and Methods This study received local IRB approval. Written informed consent was obtained from patients. We consecutively included N = 50 patients as the training cohort and a further N = 50 as the validation cohort who underwent both SSE and abdominal MR. Hepatic steatosis was classified according to MR-PDFF cutoffs as: S0 ≤ 6.5 %, S1 6.5 to 16.5 %, S2 16.5 to 22 %, S3 ≥ 22 %. Receiver operating curve analysis was performed to evaluate the diagnostic performance of SSE in the diagnosis of steatosis (S1–S3). Based on the optimal data fit derived from our study, we proposed a correspondence between the MR-PDFF and a US fat index. Coefficient of determination R2 was used to evaluate fit quality and was considered robust when R2 > 0.6. Results The training and validation cohorts presented mean SSE values of 1.570 ± 0.026 and 1.568 ± 0.023 mm/µs for S0 and 1.521 ± 0.031 and 1.514 ± 0.019 mm/µs for S1–S3 (p < 0.01) patients, respectively. An SSE threshold of ≤ 1.537 mm/µs had a sensitivity of 80 % and a specificity of 85.7 % in the diagnosis of steatosis (S1-S3) in the training cohort. Robust correspondence between MR-PDFF and the US fat index was found both for the training (R2 = 0.73) and the validation cohort (R2 = 0.76). Conclusion SSE can be used to detect, quantify and grade liver steatosis and to calculate a US fat index.

scholarly journals Research on the Correlation of Serum PCT and Plasma GSN Levels with the Prognosis of Urosepsis Patients

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Na Cui ◽  
Zhanbiao Yu ◽  
Zhi Chen ◽  
Ning Chen
Keyword(s):  
Poor Prognosis ◽  
Original Work ◽  
Protective Factor ◽  
Good Prognosis ◽  
Control Group ◽  
Creative Commons ◽  
The Poor ◽  
Prognosis Group ◽  
Good Prognosis Group ◽  
Open Access Article

Objective: To explore the correlation of procalcitonin (PCT) and gelsolin (GSN) with the prognosis of urosepsis patients. Method: The data of 71 urosepsis patients from March 2015 to April 2019 who were admitted to and treated in Affiliated Hospital of Hebei University were analyzed and compared with those of 92 healthy persons. Serum PCT and plasma GSN levels at different times after treatment were detected. According to prognosis, patients were classified into the good prognosis group or the poor prognosis group. The serum PCT and plasma GSN levels of both groups were compared. Result: The serum PCT level of the urosepsis group on the 1st, 3rd, 5th and 7th days was obviously higher than that of the control group (P<0.05). The plasma GSN levels of the urosepsis group on the 1st, 3rd, 5th and 7th days were obviously lower than those of the control group (P<0.05).The serum PCT level of the poor prognosis group on the 1st, 3rd, 5th and 7th days was obviously higher than that of the good prognosis group (P<0.05). The plasma GSN level of the poor prognosis group on the 1st, 3rd, 5th and 7th days was obviously lower than that of the good prognosis group (P<0.05). PCT was an independent risk factor influencing the prognosis of urosepsis patients and that GSN was a protective factor (P<0.05). Conclusion: The serum PCT and plasma GSN levels can accurately predict the severity and prognosis of urosepsis patients and reflect the disease state of early urosepsis patients. High PCT levels and low GSN levels indicate poor prognosis, and clinicians should consider these values. doi: https://doi.org/10.12669/pjms.36.5.2143 How to cite this:Cui N, Yu Z, Chen Z, Chen N. Research on the Correlation of Serum PCT and Plasma GSN Levels with the Prognosis of Urosepsis Patients. Pak J Med Sci. 2020;36(5):---------. doi: https://doi.org/10.12669/pjms.36.5.2143 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Analysis of ACE2 Genetic Variants in 131 Italian SARS-CoV-2 Positive Patients

2020 ◽  
Author(s):  
Antonio Novelli ◽  
Michela Biancolella ◽  
Paola Borgiani ◽  
Dario Cocciadiferro ◽  
Vito Luigi Colona ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Dna Analysis ◽  
Large Family ◽  
Population Data ◽  
Host Susceptibility ◽  
University Hospital ◽  
Control Group ◽  
Allelic Association ◽  
Similar Frequency ◽  
Whole Exome

Abstract Background: Coronaviruses (CoV) are a large family of viruses that are common in humans and many animal species. Animal coronaviruses rarely infect humans with the exceptions of the Middle East Respiratory Syndrome (MERS-CoV), the Severe Acute Respiratory Syndrome CoronaVirus (SARS-CoV), and now SARS-CoV-2, which is the cause of the ongoing pandemic of coronavirus disease 2019 (COVID-19). Several studies suggested that genetic variants in the ACE2 gene may influence the host susceptibility or resistance to SARS-CoV-2 infection according to the functional role of ACE2 in human pathophysiology. However, many of these studies have been conducted in silico based on epidemiological and population data. We therefore investigated the occurrence of ACE2 variants in a cohort of 131 Italian unrelated individuals clinically diagnosed with COVID-19 and in an Italian control population, to evaluate a possible allelic association with COVID-19, by direct DNA analysis.Methods: As a pilot study, we analyzed, by whole-exome sequencing, genetic variants of ACE2 gene in 131 DNA samples of COVID-19 patients hospitalized at Tor Vergata University Hospital and at Bambino Gesù Children’s Hospital, Rome. We used a large control group consisting of 1,000 individuals (500 males and 500 females).Results: We identified three different germline variants: one intronic c.439+4G>A and two missense c.1888G>C p.(Asp630His) and c.2158A>G p.(Asn720Asp) in a total of 131 patients with a similar frequency in male and female. Thus far, only the c.1888G>C p.(Asp630His) variant shows a statistically different frequency compared to the ethnically matched populations. Therefore, further studies are needed in larger cohorts, since it was found only in one heterozygous COVID-19 patient.Conclusions: Our results suggest that there is no strong evidence, in our cohort, of consistent association of ACE2 variants with COVID-19 severity. We might speculate that rare susceptibility/resistant alleles could be located in the non-coding regions of the ACE2 gene, known to play a role in regulation of the gene activity.

scholarly journals Role of Diffusion Tensor Imaging Combined with Neuron-Specific Enolase and S100 calcium-binding protein B Detection in Predicting the Prognosis of Moderate and Severe Traumatic Brain Injury

2021 ◽  
Keyword(s):  
Poor Prognosis ◽  
Neuron Specific Enolase ◽  
Good Prognosis ◽  
S100b Protein ◽  
Control Group ◽  
The Poor ◽  
Severe Tbi ◽  
Prognosis Group ◽  
Good Prognosis Group

Background Traumatic brain injury (TBI) seriously affects the quality of life of patients. The present study evaluated the role of diffusion tensor imaging (DTI) combined with Neuron-Specific Enolase (NSE) and S100 calcium-binding protein B (S100B) protein in predicting the prognosis of moderate and severe TBI. Methods The TBI patients were divided into moderate TBI (TBIm) and severe TBI (TBIs) groups according to the Glasgow Coma Scale (GCS) after admission. The patients were then divided into good and poor prognosis groups according to the Glasgow Outcome Scale (GOS); moreover, their follow-ups were recorded at 3 and 6 months after injury. This study also included 65 healthy individuals with matched age and gender as the control group. The fractional anisotropy (FA) values of DTI, serum neuron-specific enolase (NSE), and S100B protein levels were detected in this study. The data were analyzed in SPSS software (version 22.0) to evaluate the role of DTI combined with NSE and S100B protein in predicting the prognosis in TBIm and TBIs. Results: After TBI, the FA values of DTI in the TBI group were lower than those in the control group (P<0.05); moreover, the serum NSE and S100B values in the TBI group were higher than those in the control group (P<0.05). In the TBIm patients, the FA values of the corpus callosum in the good prognosis group were higher than that in the poor prognosis group (P<0.05); however, there was no significant difference between the two groups regarding the FA values of the internal capsule and the cerebral peduncle (P>0.05). The serum levels of NSE and S100B in the good prognosis group were significantly lower than those in the poor prognosis group (P<0.05). In the TBIs patients, the FA value of all areas in the good prognosis group was significantly higher than that in the poor prognosis group (P<0.05). However, there was no significant difference between the two prognosis groups regarding the serum levels of NSE and S100B (P>0.05). Conclusion Although DTI combined with NSE and S100B protein can effectively predict the prognosis of patients with moderate and severe TBI in the early stages, various other measures have been used in the studies to predict the prognosis of TBI patients. Accordingly, comparison with other measures is essential in further studies.

Dorsal intercalated segmental instability associated with malunion of a reconstructed scaphoid

2016 ◽  
Vol 42 (3) ◽  
pp. 240-245 ◽  
Author(s):  
J.-H. Kim ◽  
K.-H. Lee ◽  
B. G. Lee ◽  
C.-H. Lee ◽  
S.-J. Kim ◽  
...  
Keyword(s):  
Scaphoid Fracture ◽  
Length Ratio ◽  
Receiver Operating Curve ◽  
Flexion Deformity ◽  
Level Of Evidence ◽  
Tomographic Images ◽  
Segmental Instability ◽  
Computed Tomographic ◽  
Receiver Operating Curve Analysis ◽  
Fracture Nonunion

We analysed scaphoid deformity as a result of surgical treatment of scaphoid fracture nonunion and assessed the deformity associated with a dorsal intercalated segmental instability pattern of carpal malalignment. A total of 45 patients who were treated for scaphoid fracture nonunion were included in the study. The height-to-length ratio of the scaphoid was measured on computed tomographic images and used to assess scaphoid deformity. Carpal malalignment was quantified based on the radio-lunate angle. A correlation analysis between the height-to-length ratio and the radio-lunate angle was performed. Dorsal intercalated segmental instability was defined as a radio-lunate angle >15°, and a receiver operating curve analysis was used to calculate the cutoff height-to-length ratio that can be accompanied with dorsal intercalated segmental instability. Extension of the lunate increases in proportion to the flexion deformity of the scaphoid; dorsal intercalated segmental instability can occur if the height-to-length ratio of the scaphoid is >0.73. Level of evidence: IV

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