scholarly journals Evidence for significant hematopoiesis in the human thymus

Blood ◽  
1976 ◽  
Vol 47 (2) ◽  
pp. 305-313 ◽  
Author(s):  
CR Taylor ◽  
JM Skinner
Keyword(s):  
Plasma Cells ◽  
Positive Staining ◽  
Immunoperoxidase Method ◽  
Thymic Tissue ◽  
Fetal Thymus ◽  
Neonatal Deaths ◽  
Hemoglobin A ◽  
Staining Techniques ◽  
Immunohistochemical Methods ◽  
Indirect Immunoperoxidase

Abstract Earlier studies on fetal thymus suggested that certain of the large pyroninophilic cells found there might have a hemopoietic role, and it was decided to determine the nature of these cells using histochemical and immunohistochemical methods. Thymic tissue from aborted fetuses, stillbirths, and neonatal deaths was examined histochemically using methods for the detection of chloroacetate esterase, peroxidase, and pseudoperoxidase, and by staining techniques for mast cells and eosinophils. Tissue was also examined using the indirect immunoperoxidase method for the presence of hemoglobin A (HbA) and F (HbF), for lysozyme (muramidase) and immunoglobins alpha, mu, gamma, kappa, lambda. Positive staining to some degree was seen in cells in the connective tissue stroma using all methods, and the cells stained corresponded to one or another of the types of pyroninophilic cells present. The finding of large cells with positive chloroacetate esterase and antilysozyme indicates the presence of granulopoiesis. Similarly, the presence of large nucleated cells with pseudoperoxidase and anti-hemoglobin (A and F) staining indicates the presence of erythropoiesis. Plasma cells were present in small numbers.

scholarly journals Evidence for significant hematopoiesis in the human thymus

Blood ◽  
1976 ◽  
Vol 47 (2) ◽  
pp. 305-313
Author(s):  
CR Taylor ◽  
JM Skinner
Keyword(s):  
Plasma Cells ◽  
Positive Staining ◽  
Immunoperoxidase Method ◽  
Thymic Tissue ◽  
Fetal Thymus ◽  
Neonatal Deaths ◽  
Hemoglobin A ◽  
Staining Techniques ◽  
Immunohistochemical Methods ◽  
Indirect Immunoperoxidase

Earlier studies on fetal thymus suggested that certain of the large pyroninophilic cells found there might have a hemopoietic role, and it was decided to determine the nature of these cells using histochemical and immunohistochemical methods. Thymic tissue from aborted fetuses, stillbirths, and neonatal deaths was examined histochemically using methods for the detection of chloroacetate esterase, peroxidase, and pseudoperoxidase, and by staining techniques for mast cells and eosinophils. Tissue was also examined using the indirect immunoperoxidase method for the presence of hemoglobin A (HbA) and F (HbF), for lysozyme (muramidase) and immunoglobins alpha, mu, gamma, kappa, lambda. Positive staining to some degree was seen in cells in the connective tissue stroma using all methods, and the cells stained corresponded to one or another of the types of pyroninophilic cells present. The finding of large cells with positive chloroacetate esterase and antilysozyme indicates the presence of granulopoiesis. Similarly, the presence of large nucleated cells with pseudoperoxidase and anti-hemoglobin (A and F) staining indicates the presence of erythropoiesis. Plasma cells were present in small numbers.

scholarly journals Leu-7 immunoreactivity in human, monkey, and pig bronchopulmonary neuroepithelial bodies and neuroendocrine cells.

1987 ◽  
Vol 35 (6) ◽  
pp. 687-691 ◽  
Author(s):  
J M Lauweryns ◽  
L Van Ranst
Keyword(s):  
Monoclonal Antibody ◽  
Nerve Fibers ◽  
Neuroendocrine Cells ◽  
Immunoperoxidase Method ◽  
Neuroepithelial Bodies ◽  
Immune Systems ◽  
Myelin Sheaths ◽  
Human Natural Killer Cells ◽  
Indirect Immunoperoxidase ◽  
Leu 7

Anti-Leu 7 is a monoclonal antibody recognizing a surface antigen on human natural killer cells. By applying the indirect immunoperoxidase method, we demonstrated Leu-7 immunoreactivity in the cytoplasm of neuroepithelial bodies (NEB) and neuroendocrine cells (NEC) of human, monkey, and pig respiratory mucosa. In addition, the anti-Leu-7 monoclonal antibody stained the myelin sheaths of nerve fibers in all tissues investigated. Our findings support the hypothesis that shared antigens exist between the nervous, endocrine, and immune systems.

Increased Expression of Cyclin-D1 on Trephine Bone Marrow Biopsies Independently Predicts for Shorter Overall Survival In Patients with Multiple Myeloma Treated with Novel Agents

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 2965-2965
Author(s):  
Evangelos Terpos ◽  
Maria Roussou ◽  
Anna Tasidou ◽  
Magdalini Migkou ◽  
Maria Gavriatopoulou ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Overall Survival ◽  
Cyclin D1 ◽  
Plasma Cells ◽  
Univariate Analysis ◽  
Novel Agents ◽  
Positive Staining ◽  
Newly Diagnosed ◽  
Immunohistochemical Expression

Abstract Abstract 2965 The cyclin-D1 proto-oncogene is an important cell regulator of G1 to S phase progression. The overexpression of cyclin-D1 has been linked to the development and progression of several malignancies. The aim of our study was to evaluate the impact of the immunohistochemical expression of cyclin-D1on the plasma cells of trephine biopsies on survival of newly-diagnosed patients with multiple myeloma (MM) who were treated with novel agents. We evaluated formalin-fixed, paraffin-embedded, bone marrow sections of 130 consecutive patients with newly-diagnosed MM (67M/63F; median age 68 years) before any kind of therapy administration. One hundred and fifteen patients had symptomatic disease that required therapy: 29 (25%) received bortezomib-based regimens and 31 (26%) received thalidomide-based regimens as first line therapy, while all patients received regimens containing bortezomib or an IMiD at some point during the course of their disease. Immunohistochemistry was performed in all trephine biopsies using monoclonal antibodies against cyclin-D1 (Cell Marque Corp., Rocklin, CA, USA), but also against CD56 (Cell Marque Corp., Rocklin, CA, USA), CD27 (Novocastra, Newcastle upon Tyne, UK), CD117 and MUM-1 (DAKO A/S, Glostrup, Denmark), as recommended by the manufacturers. A case was considered positive if there was unequivocal positive staining of at least 20% of the plasma cells for cyclin-D1, CD56 and MUM-1 and a positive staining of at least 10% of the plasma cells for CD117 and CD27. Among patients with symptomatic myeloma (N=115), positive staining for cyclin-D1 was found in 35 (30%) patients, for CD56 in 45 (39%), for CD117 in 94 (81%) and for CD27 in 72 (62%) patients. In patients with asymptomatic myeloma, positive staining for Cyclin-D1 was found only in 1 (7%) patient, for CD56 in 9 (64%), and for CD117 in 6 (43%) (p<0.01 for all comparisons compared to symptomatic patients). There were significant positive correlations between positivity for CD27 and CD56 (p<0.001), between positivity for cyclin-D1 and CD117 (p=0.045) and a negative correlation between positivity for CD117 and CD56 (p=0.001). We also observed significant correlations between CD56 positivity and ISS-1 or ISS-2 (p=0.01) and between CD117 positivity and ISS-3 disease (p=0.002). The median overall survival (OS) for patients with symptomatic MM was 57 months (range 22–120 months). In the univariate analysis, positivity for cyclin-D1 (41 vs. 62 months, p=0.03) and for CD117 (50 vs. 75 months p=0.018) were associated with inferior survival, while positivity for CD56 (47 vs. 62 months, p=0.286), MUM-1 (52.7 vs. 63.8 months, p=0.528) and CD27 (57 vs. 50 months, p=0.445) were not. Other factors associated with inferior OS, in the univariate analysis, included ISS-3 (median OS 37 months, vs. 57 months for ISS-2 and 73 months for ISS-1, p=0.005), Hb <10 g/dl (56 vs. 73 months, p=0.044), corrected serum calcium >11.5 g/dl (29 vs. 62 months, p=0.02), serum LDH above upper normal limit (31 vs. 61 months, p=0.05), serum creatinine >2 mg/dl (26 vs. 64 months, p=0.007), low platelet counts (<100,000/ml) (22 vs. 62 months, p=0.031) and age >65 years (45 months vs. not reached for younger patients, p=0.002). In the multivariate analysis, positivity for cyclin-D1 (HR: 2.6; p=0.001), ISS stage (HR: 1.8; p=0.001) and age >65 (HR 2.7, p=0.003) were independently associated with inferior survival. Immunohistochemistry for cyclin-D1 identified subgroups of patients in ISS-2 and in ISS-3 who had extremely poor outcome. Patients with cyclin-D1 positivity had a median survival of 22 months in ISS-2 (vs. 64 months for the rest of ISS-2 patients, p=0.01) and of 13 months in ISS-3 (vs. 47 months for the rest of ISS-3, p=0.012). Our findings underline that the immunohistochemical expression of cyclin-D1 in the bone marrow trephine biopsies has independent prognostic value in MM patients, even in the era of novel agents. This marker can easily be assessed in patients who undergo a trephine biopsy as part of their initial evaluation and offers significant prognostic information. Furthermore, novel agents targeting cyclin-D1 may be of therapeutic value in MM. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Detection of purine nucleoside phosphorylase in paraffin sections by the immunoperoxidase method.

1984 ◽  
Vol 32 (7) ◽  
pp. 783-787
Author(s):  
G Itoh ◽  
H Ikeda ◽  
M Nakano ◽  
H Baba ◽  
Y Hayashi
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Plasma Cells ◽  
Rabbit Antiserum ◽  
Staining Intensity ◽  
Germinal Centers ◽  
Purine Nucleoside ◽  
Immunoperoxidase Method ◽  
Paraffin Sections ◽  
Nucleoside Phosphorylase ◽  
Pap Method

A method is described for immunohistochemical demonstration of purine nucleoside phosphorylase (PNP: EC 2.4.2.1) in paraffin sections from routine surgical histology specimens. A peroxidase-antiperoxidase (PAP) method was employed, using specific rabbit antiserum against human PNP, which was purified from postmature human erythrocytes. In human lymph nodes, intensive staining for PNP was observed in the vast majority of small lymphocytes in paracortical areas, in many small lymphocytes in medullary cords, and in a few small-to medium-sized lymphocytes in germinal centers. Small lymphocytes in the primary follicles and those in the mantle zones of secondary follicles were negative for PNP staining. Tingible body macrophages, lymphatic sinus cells, and most of the large cells in germinal centers did not stain with anti-human PNP (hPNP) antibody. Endothelial cells of small vessels in the cortex and plasma cells did not show any constant pattern of PNP staining intensity. Histochemistry revealed that the distribution pattern of PNP activity was quite similar to that demonstrated on paraffin sections by the PAP method.

Viral Antigen Distribution in Organs of Cattle Experimentally Infected with Rinderpest Virus

1993 ◽  
Vol 30 (6) ◽  
pp. 544-554 ◽  
Author(s):  
P. Wohlsein ◽  
G. Trautwein ◽  
T. C. Harder ◽  
B. Liess ◽  
T. Barrett
Keyword(s):  
Viral Antigen ◽  
Virulent Strain ◽  
Immunoperoxidase Method ◽  
Rinderpest Virus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
The Central Nervous System ◽  
Distribution In Organs ◽  
Indirect Immunoperoxidase

The distribution of viral antigen in various organs of four approximately 10-month-old castrated male Friesian cattle experimentally infected with a highly virulent strain of rinderpest virus was studied. A monoclonal antibody with genus-specific reactivity for morbilliviruses was applied in an indirect immunoperoxidase method performed on formalin-fixed, paraffin-embedded tissue sections. Rinderpest viral antigen was located mainly in the cytoplasm of the epithelial cells of the digestive, respiratory, and urinary tracts, as well as in the cells of endocrine glands (adrenal, thyroid) and exocrine glands (salivary glands, sebaceous glands, exocrine pancreas). Furthermore, different types of cells in lymphatic organs contained rinderpest viral antigen. In contrast to the documented results of studies carried out with other morbilliviruses, tissues of the central nervous system did not contain viral antigen. Various types of epithelial and lymphoreticular cells are the main targets of a virulent strain of rinderpest virus in vivo.

scholarly journals Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Shirin Karimi ◽  
Masoud Shamaei ◽  
Mihan Pourabdollah ◽  
Makan Sadr ◽  
Mehrdad Karbasi ◽  
...  
Keyword(s):  
Immunohistochemical Staining ◽  
Plasma Cells ◽  
Tissue Reaction ◽  
Positive Staining ◽  
National Research Institute ◽  
Diagnostic Challenge ◽  
Tissue Samples ◽  
Granulomatous Tissue ◽  
Immunohistological Staining ◽  
Mycobacterial Antigens

Purpose. The histological diagnosis of Mycobacterium tuberculosis (MTB) remains a diagnostic challenge despite different methods. Immunohistochemistry (IHC) not only could confirm granulomatous tissue involvement but also can demonstrate MTB antigen immunolocalization. This study tries to clarify the details of immunohistochemical staining for MTB with pAbBCG.Materials/Methods. Twenty-three confirmed TB granulomatous tissue samples were studied by Ziehl-Neelsen and immunohistochemistry (IHC) staining with pAbBCG. Samples were selected from the archive of the Department of Pathology, National Research Institute of Tuberculosis and Lung Disease, Tehran, Iran.Results. IHC staining was positive in all samples, whereas Ziehl-Neelsen was positive in 9 cases out of 23 (39.1%). Tissue types used were pleural tissue, lymph nodes, and lung tissue. IHC showed positive coarse granular cytoplasmic and round, fragmented bacillary staining. In this study, epithelioid cells clearly showed more positive staining at the periphery of the granuloma rather than the center of granuloma. There is also positive staining in endothelial cells, fibroblasts, plasma cells, lymphocytes, and macrophages outside the granuloma.Conclusion. Considering the criteria of positive immunohistochemical staining of TB granulomatous reactions, this stain not only highlights the presence of mycobacterial antigens for tissue diagnosis, but also could morphologically localize its distribution in different cells.

scholarly journals Use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection

1977 ◽  
Vol 6 (6) ◽  
pp. 571-573
Author(s):  
D R Benjamin
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Infection ◽  
Reliable Method ◽  
Herpes Simplex Virus Infection ◽  
Rapid Diagnosis ◽  
Immunoperoxidase Method ◽  
Infected Cells ◽  
Indirect Immunoperoxidase ◽  
Simplex Virus

The indirect immunoperoxidase method was applied to the rapid diagnosis of mucocutaneous herpes simplex virus infection. Specimens were obtained directly from lesions at various stages in their evolution. The immunoperoxidase method identified herpes simplex virus-infected cells in 91% of vesicular lesions which were positive by standard virological culture. At later stages, the number of cases that could be diagnosed decreased appreciably. The immunoperoxidase method appears to be a rapid and reliable method for the diagnosis of early herpes simplex virus infection.

Immunohistochemical Demonstration of Desmin in Canine Smooth Muscle Tumors

1987 ◽  
Vol 24 (3) ◽  
pp. 211-215 ◽  
Author(s):  
C. B. Andreasen ◽  
E. A. Mahaffey
Keyword(s):  
Smooth Muscle ◽  
Positive Staining ◽  
Negative Results ◽  
Formalin Fixed Paraffin ◽  
Smooth Muscle Tumors ◽  
Formalin Fixed Paraffin Embedded ◽  
Immunohistochemical Methods ◽  
Formalin Fixed ◽  
Immunohistochemical Identification ◽  
Avidin Biotin Complex

Sections of formalin-fixed, paraffin-embedded canine leiomyomas, leiomyosarcomas, or fibrosarcomas were examined by immunohistochemical methods for the presence of desmin. Twenty-two leiomyomas and leiomyosarcomas were stained using the avidin-biotin complex technique, and 14 samples demonstrated positive staining for desmin. The eight negative results obtained may reflect differences in fixation or the affinity of the primary antibody for the tissues examined. Desmin was specific for myogenic tissues. Five canine fibrosarcomas examined immunohistochemically were all negative for desmin staining. The results indicate that desmin is a useful marker for immunohistochemical identification of canine leiomyomas and leiomyosarcomas.

scholarly journals ULTRASTRUCTURE OF SARCOPLASMIC RETICULUM PREPARATIONS

1969 ◽  
Vol 42 (1) ◽  
pp. 296-307 ◽  
Author(s):  
D. W. Deamer ◽  
R. J. Baskin
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Oxalate ◽  
Calcium Transport ◽  
Osmium Tetroxide ◽  
Positive Staining ◽  
Rabbit Muscle ◽  
Calcium Oxalate Crystals ◽  
Electron Microscopic ◽  
Staining Techniques ◽  
Microscopic Techniques

Fragmented sarcoplasmic reticulum (FSR) from rabbit muscle was examined by positive staining, negative staining, and freeze-etch electron microscopic techniques in the absence and presence of calcium transport conditions. The existence of 30–40 A particles covering the outer surface of FSR vesicles was confirmed by two different negative stains in unfixed, glutaraldehyde-fixed and osmium tetroxide-fixed material. Freeze-etch microscopy revealed a second type of particle, 80–90 A in diameter, on the fractured surfaces of FSR vesicles. Following calcium oxalate accumulation, negative and positive staining techniques provided evidence for large nodular deposits within FSR vesicles which probably correspond to calcium oxalate crystals and are responsible for increments in turbidity during calcium oxalate accumulation. The most probable configuration of FSR vesicles in solution is spherical. "Tadpole" or tubular configurations were not seen by freeze-etch microscopy, positive staining, or in prefixed negatively stained material.

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