scholarly journals Inhibition of CFU-NM and CFU-EOS by mature granulocytes

Blood ◽  
1984 ◽  
Vol 63 (2) ◽  
pp. 376-379
Author(s):  
BH Bjornson ◽  
SH Pincus ◽  
AM DiNapoli ◽  
JF Desforges
Keyword(s):  
Mononuclear Cells ◽  
Feedback Inhibition ◽  
Feeder Layer ◽  
Intact Cells ◽  
Normal Peripheral Blood ◽  
Inhibition Of Growth ◽  
Feeder Layers ◽  
Eosinophil Colonies ◽  
Differential Control

Approximately half of the colony-forming units-culture (CFU-C) from normal peripheral blood are eosinophilic. The purpose of our study was to determine: (1) whether progenitor cells committed to eosinophil or neutrophil maturation would be differentially affected by feedback inhibition, and (2) whether mature eosinophils added to the feeder layers of the culture would inhibit the proliferation of CFU-C in a manner similar to that described for neutrophils. Concentrated eosinophils and neutrophils, obtained by separation on a metrizamide gradient, were added to feeder layers containing either 10(6) autologous whole mononuclear cells (WMNC) or 0.1 ml of leukocyte conditioned media (LCM). The average number of colonies was 123/10(6) nonadherent cells (NAC) cultured. When neutrophils or eosinophils were added to the WMNC feeder layer, the percent inhibition of growth was 40.2% +/- 1.6% (mean +/- SEM) and 42.3% +/- 5.4%, respectively, but the ratio of neutrophil to eosinophil colonies remained constant. No effect was seen when neutrophils or eosinophils were added to an LCM feeder layer. Thus, it appears that the differential control of neutrophil versus eosinophil production in vitro is not regulated through feedback inhibition by mature granulocytes. In addition, these studies suggest that eosinophils, as well as neutrophils, cause inhibition of CFU-C growth when intact cells are the source of colony-stimulating factor (CSF).

scholarly journals Inhibition of CFU-NM and CFU-EOS by mature granulocytes

Blood ◽  
1984 ◽  
Vol 63 (2) ◽  
pp. 376-379 ◽  
Author(s):  
BH Bjornson ◽  
SH Pincus ◽  
AM DiNapoli ◽  
JF Desforges
Keyword(s):  
Mononuclear Cells ◽  
Feedback Inhibition ◽  
Feeder Layer ◽  
Intact Cells ◽  
Normal Peripheral Blood ◽  
Inhibition Of Growth ◽  
Feeder Layers ◽  
Eosinophil Colonies ◽  
Differential Control

Abstract Approximately half of the colony-forming units-culture (CFU-C) from normal peripheral blood are eosinophilic. The purpose of our study was to determine: (1) whether progenitor cells committed to eosinophil or neutrophil maturation would be differentially affected by feedback inhibition, and (2) whether mature eosinophils added to the feeder layers of the culture would inhibit the proliferation of CFU-C in a manner similar to that described for neutrophils. Concentrated eosinophils and neutrophils, obtained by separation on a metrizamide gradient, were added to feeder layers containing either 10(6) autologous whole mononuclear cells (WMNC) or 0.1 ml of leukocyte conditioned media (LCM). The average number of colonies was 123/10(6) nonadherent cells (NAC) cultured. When neutrophils or eosinophils were added to the WMNC feeder layer, the percent inhibition of growth was 40.2% +/- 1.6% (mean +/- SEM) and 42.3% +/- 5.4%, respectively, but the ratio of neutrophil to eosinophil colonies remained constant. No effect was seen when neutrophils or eosinophils were added to an LCM feeder layer. Thus, it appears that the differential control of neutrophil versus eosinophil production in vitro is not regulated through feedback inhibition by mature granulocytes. In addition, these studies suggest that eosinophils, as well as neutrophils, cause inhibition of CFU-C growth when intact cells are the source of colony-stimulating factor (CSF).

scholarly journals The effect of serum on monocyte tissue factor generation

Blood ◽  
1984 ◽  
Vol 64 (3) ◽  
pp. 707-714 ◽  
Author(s):  
RL Edwards ◽  
D Perla
Keyword(s):  
T Cells ◽  
Tissue Factor ◽  
Mononuclear Cells ◽  
High Serum ◽  
Peripheral Blood Mononuclear ◽  
Serum Factors ◽  
Normal Peripheral Blood ◽  
Stimulatory Activity ◽  
Normal Hemostasis

Abstract Human monocytes generate the procoagulant tissue factor (MTF) following exposure to a variety of immune stimuli in vitro. The generation of MTF is modified by T cells, lymphokines, and immunoregulatory lipoproteins, and recent studies have shown that MTF can be activated in an immune- specific manner following exposure to antigen. We have examined the role of serum factors in the regulation of MTF generation. Low concentrations (less than 1%) of heat-inactivated normal human serum greatly enhanced MTF generation in cultures of normal peripheral blood mononuclear cells. The stimulatory effect was observed in cultures of both unstimulated cells and cells exposed to bacterial lipopolysaccharide. Stimulation was not observed at high serum concentrations (greater than 10%) and could not be explained by endotoxin contamination or activation of the assay system. Stimulatory activity was present in plasma and BaSO4-adsorbed plasma as well as autologous and allogeneic serum, was not abolished by removal of serum lipoproteins, and did not require the presence of T cells for its expression. Sera from 28 different normal volunteers were screened for stimulatory activity and demonstrated a wide variation in potency. These results suggest that a potent factor is present in sera that enhances the expression of MTF activity in vitro. This factor is distinct from previously described lipoprotein regulators and may play a role in the initiation of coagulation in both normal hemostasis and pathologic states.

scholarly journals Preclinical Antileukemia Activity of Tramesan: A Newly Identified Bioactive Fungal Metabolite

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
M. R. Ricciardi ◽  
R. Licchetta ◽  
S. Mirabilii ◽  
M. Scarpari ◽  
A. Parroni ◽  
...  
Keyword(s):  
Cell Lines ◽  
Molecular Mechanisms ◽  
Mononuclear Cells ◽  
Leukemic Cell ◽  
Bioactive Compound ◽  
Primary Cells ◽  
Peripheral Blood Mononuclear ◽  
Normal Peripheral Blood ◽  
Leukemic Cell Lines

Despite improvements that occurred in the last decades in the acute myeloid leukemia (AML) treatment, clinical results are still unsatisfactory. More effective therapies are required, and innovative approaches are ongoing, including the discovery of novel antileukemia natural compounds. Several studies have described the activity of extracts from mushrooms which produce compounds that exhibited immunological and antitumor activities. The latter has been demonstrated to be promoted in vitro by mushroom polysaccharides via induction of apoptosis. However, the antileukemia activity of these compounds on primary cells is still not reported. In the present study, we examined the in vitro effects of Tramesan (TR), a bioactive compound extracted from Trametes versicolor, on leukemic cell lines and primary cells. Our results demonstrated that TR induced a marked growth inhibition of leukemic cell lines and primary cells from AML patients. The antiproliferative effects of TR were associated in primary AML cells with a significant increase of apoptosis. No significant cytotoxic effects were observed in normal peripheral blood mononuclear cells (MNC) from healthy donors. Our data demonstrated a cytotoxic activity of TR on leukemia cells prompting further translational applications. Ongoing studies are elucidating the molecular mechanisms underlying its antileukemic activity.

scholarly journals Oncogene expression in autoimmune and normal peripheral blood mononuclear cells.

1986 ◽  
Vol 163 (5) ◽  
pp. 1292-1307 ◽  
Author(s):  
D M Klinman ◽  
J F Mushinski ◽  
M Honda ◽  
Y Ishigatsubo ◽  
J D Mountz ◽  
...  
Keyword(s):  
B Cells ◽  
Mononuclear Cells ◽  
Cell Types ◽  
Isolated Cells ◽  
Peripheral Blood Mononuclear ◽  
Normal Peripheral Blood ◽  
Additional Cell ◽  
Normal Controls

PBMC from patients with autoimmune diseases and from normal controls were studied for the expression of several cellular oncogenes. Gene expression was assessed by Northern blot analysis of poly(A)+ RNA obtained from leukapheresis samples. Patients with SLE expressed significantly more c-myc protooncogene RNA than did normal controls. Increased expression of the N-ras protooncogene was found in that subset of patients whose autoimmune disease was very active. Cells from individuals with SLE, but not from those with other autoimmune illnesses, showed significantly decreased levels of the c-myb and c-fos protooncogenes. To examine the implications of these findings, B and T cells were purified from apheresis samples donated by normal volunteers. When mitogen was used to activate the B cells in vitro, their pattern of protooncogene expression changed to resemble that found in freshly isolated cells from lupus patients. These results suggest that the differences detected in the expression of protooncogenes by patients with SLE may be due to the abnormal activation of their B cells in vivo. The pattern of protooncogene expression found in patients with other autoimmune illnesses is consistent with the activation of additional cell types in those diseases.

scholarly journals Regulation of in vitro erythropoiesis by normal T cells: evidence for two T-cell subsets with opposing function

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 171-174 ◽  
Author(s):  
B Torok-Storb ◽  
PJ Martin ◽  
JA Hansen
Keyword(s):  
T Cells ◽  
Mononuclear Cells ◽  
T Cell Subsets ◽  
Cellular Interactions ◽  
Normal Peripheral Blood ◽  
Human Mononuclear Cells ◽  
Complement Dependent Cytotoxicity ◽  
Regulatory Effects ◽  
Murine Monoclonal Antibodies

Cellular interactions responsible for regulating in vitro erythropoiesis were studied using murine monoclonal antibodies recognizing antigens expressed by human mononuclear cells. Cell populations of interest were negatively selected by complement- dependent cytotoxicity and then evaluated for their effect on in vitro growth of erythroid burst-forming units (BFU-E). The data suggest that normal peripheral blood T cells contain at least two functionally distinct subpopulations with opposing regulatory effects: one that enhances burst formation had one that limits burst formation. Whether these effects are mediated by direct interactions of T cells with BFU-E or with auxillary cells remains to be determined.

scholarly journals Regulation of in vitro erythropoiesis by normal T cells: evidence for two T-cell subsets with opposing function

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 171-174 ◽  
Author(s):  
B Torok-Storb ◽  
PJ Martin ◽  
JA Hansen
Keyword(s):  
T Cells ◽  
Mononuclear Cells ◽  
T Cell Subsets ◽  
Cellular Interactions ◽  
Normal Peripheral Blood ◽  
Human Mononuclear Cells ◽  
Complement Dependent Cytotoxicity ◽  
Regulatory Effects ◽  
Murine Monoclonal Antibodies

Abstract Cellular interactions responsible for regulating in vitro erythropoiesis were studied using murine monoclonal antibodies recognizing antigens expressed by human mononuclear cells. Cell populations of interest were negatively selected by complement- dependent cytotoxicity and then evaluated for their effect on in vitro growth of erythroid burst-forming units (BFU-E). The data suggest that normal peripheral blood T cells contain at least two functionally distinct subpopulations with opposing regulatory effects: one that enhances burst formation had one that limits burst formation. Whether these effects are mediated by direct interactions of T cells with BFU-E or with auxillary cells remains to be determined.

Synovium-derived stromal cell-induced osteoclastogenesis: a potential osteoarthritis trigger

2019 ◽  
Vol 133 (16) ◽  
pp. 1813-1824
Author(s):  
Manuela Dicarlo ◽  
Gabriella Teti ◽  
Giorgia Cerqueni ◽  
Iolanda Iezzi ◽  
Antonio Gigante ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Stromal Cell ◽  
Mononuclear Cells ◽  
In Vitro Study ◽  
Holistic Approach ◽  
Peripheral Blood Mononuclear ◽  
Differentiation Potential ◽  
Normal Peripheral Blood ◽  
Shed Light

Abstract Purpose: To shed light on the idea that mesenchymal stem/stromal cells (MSCs) recruited in synovium (SM) (i.e. Synovium-Derived Stromal Cells, SDSCs) could be involved in Osteoarthritis (OA) pathophysiology. Attention was also paid to a further stromal cell type with a peculiar ultrastructure called telocytes (TCs), whose role is far from clarified. Methods: In the present in vitro study, we compared SDSCs isolated from healthy and OA subjects in terms of phenotype, morphology and differentiation potential as well as in their capability to activate normal Peripheral Blood Mononuclear Cells (PBMCs). Histological, immunohistochemical and ultrastructural analyses were integrated by qRT-PCR and functional resorbing assays. Results: Our data demonstrated that both SDSC populations stimulated the formation of osteoclasts from PBMCs: the osteoclast-like cells generated by healthy-SDSCs via transwell co-cultures were inactive, while OA-derived SDSCs have a much greater effectiveness. Moreover, the presence of TCs was more evident in cultures obtained from OA subjects and suggests a possible involvement of these cells in OA. Conclusions: Osteoclastogenic differentiation capability of PBMCs from OA subjects, also induced by B synoviocytes has been already documented. Here we hypothesized that SDSCs, generally considered for their regenerative potential in cartilage lesions, have also a role in the onset/maintenance of OA. Clinical relevance: Our observations may represent an interesting opportunity for the development of a holistic approach for OA treatment, that considers the multifaceted capability of MSCs in relation to the environment.

Molecular Mechanisms Responsible for In Vitro Cytotoxic Attributes of Conyza bonariensis Extract against Lymphoblastic Leukaemia Jurkat Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Saleem ◽  
Valerie B Schini-Kerth ◽  
Khalid Hussain ◽  
Syed Haroon Khalid ◽  
Muhammad Asif ◽  
...  
Keyword(s):  
Western Blot ◽  
Cytotoxic Activity ◽  
Molecular Mechanisms ◽  
Mononuclear Cells ◽  
Lymphoblastic Leukemia ◽  
Jurkat Cells ◽  
Human Leukemia ◽  
Normal Peripheral Blood ◽  
Conyza Bonariensis

Background: Conyza bonariensis is known to have anti-cancer properties. Objective: The study investigated the in vitro pro-apoptotic properties of Conyza bonariensis (C. bonariensis) towards human lymphoblastic leukemia Jurkat cells. Methods: C. bonariensis are extracted with non-polar solvent by maceration. MTS cell viability assay was employed to determine the cytotoxic activity of the extract towards human leukemia Jurket cells and normal Peripheral Blood Mononuclear Cells (PBMCs) cells. The phytochemical composition of the extract was chemically characterized using HPLC. Flow cytometric studies (FACS) were conducted to explore the pro-apoptotic potential of the extract. Western blot studies were employed to identify the molecular targets involved in the induction of apoptosis. Results: The n-hexane extract showed selective cytotoxic activity towards Jurkat cells. FACS analysis indicated that the extract induced early and late apoptosis in Jurkat cells. Western blot studies revealed that the extract down-regulated the expression of DNMT1, SIRT1, and UHRF1 with a simultaneous up-regulation of the expression of p73 and caspases-3 proteins. HPLC characterization of the extract revealed the presence of phenolic compounds. Conclusion: Overall these findings demonstrate that the anticancer effects of a Conyza bonariensis extract towards human lymphoblastic leukemiais due to the modulation of the activity of multiple oncogenic and tumor suppressor proteins and that its phenolic content is involved are proposed to be responsible for these activities.

scholarly journals Some Factors Influencing Granulocytic Colony Formation In Vitro by Human White Blood Cells

Blood ◽  
1974 ◽  
Vol 44 (4) ◽  
pp. 511-515 ◽  
Author(s):  
Wolfgang Heit ◽  
Peter Kern ◽  
Bernhard Kubanek ◽  
Hermann Heimpel
Keyword(s):  
Colony Formation ◽  
Mononuclear Cells ◽  
White Blood Cells ◽  
Soft Agar ◽  
Red Cell ◽  
Neutrophilic Granulocytes ◽  
Feeder Layers ◽  
Granulocytic Colony ◽  
Human White Blood Cells

Abstract The experiments presented in this paper were designed to characterize the colony-stimulating activity (CSA) from white blood cell feeder layers on colony formation by peripheral blood CFCs in soft agar double-layer cultures. The data confirm earlier observations that mononuclear cells are essential for CSA production but document the importance of neutrophilic granulocytes to enhance colony formation. Possible differences between colony enhancement by granulocytes and red cell hemolysate are discussed.

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