scholarly journals Investigation of an Escherichia coli O145 outbreak in a child day-care centre - extensive sampling and characterization of eae- and stx 1-positive E. coli yields epidemiological and socioeconomic insight

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Erik Wahl ◽  
Line Vold ◽  
Bjørn A Lindstedt ◽  
Torkjel Bruheim ◽  
Jan E Afset
Keyword(s):  
Escherichia Coli ◽  
Day Care ◽  
Care Centre ◽  
E Coli ◽  
Child Day Care ◽  
Day Care Centre

scholarly journals Prevalence of day-care centre children (France) with faecal CTX-M-producing Escherichia coli comprising O25b:H4 and O16:H5 ST131 strains

2014 ◽  
Vol 69 (5) ◽  
pp. 1231-1237 ◽  
Author(s):  
V. Blanc ◽  
V. Leflon-Guibout ◽  
J. Blanco ◽  
M. Haenni ◽  
J.-Y. Madec ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Day Care ◽  
Care Centre ◽  
Day Care Centre

scholarly journals Outbreak of cryptosporidiosis in a child day-care centre in Gipuzkoa, Spain, October to December 2011

2012 ◽  
Vol 17 (5) ◽  
Author(s):  
J Artieda ◽  
M Basterrechea ◽  
L Arriola ◽  
M Yagüe ◽  
E Albisua ◽  
...  
Keyword(s):  
Day Care ◽  
Attack Rate ◽  
Care Centre ◽  
Child Day Care ◽  
Cryptosporidium Oocysts ◽  
Day Care Centre ◽  
Infection Spread

From October to December 2011, an outbreak of 26 cases of cryptosporidiosis occurred in a day-care centre in Gipuzkoa, Spain. The infection spread from person to person and affected 24 children under two years of age (attack rate: 38%) and two caregivers. Cryptosporidium oocysts were observed in 10 of 15 samples. During 2010, only four cases of cryptosporidium were detected in Gipuzkoa, and 27 overall in Spain.

FACTORS DETERMINING VULNERABILITY ON ONLINE SOCIAL NETWORKS AMONG TEENAGE GIRLS ATTENDING A CHILD DAY CARE CENTRE

2018 ◽  
Vol 3 ◽  
pp. 442-458
Author(s):  
Lilia Žukauskienė ◽  
Rasa Skališienė
Keyword(s):  
Social Networks ◽  
Grounded Theory ◽  
Online Social Networks ◽  
Day Care ◽  
Care Centre ◽  
Teenage Girls ◽  
Child Day Care ◽  
One Step ◽  
Intervening Factors ◽  
Day Care Centre

The article presents the results of the qualitative research which provide a deeper understanding of the factors determining vulnerability on online social networks among teenage girls attending a Child Day Care Centre. The research has involved the specialists from Child Day Care Centres of different towns and districts of Lithuania. A version of a systematic grounded theory (Strauss & Corbin, 1990) has been applied to conduct the research; it has helped to construct the phenomenon – Insight into threats on online social networks: “One step and everything can end up badly”. By applying the paradigmatic model of “cause and effect” of the systematic grounded theory, reasons for the development of this phenomenon, contextual and intervening factors, strategies of actions/interactions applied by the specialists as well as the manifested consequences have been distinguished.

scholarly journals Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masuzu Kikuchi ◽  
Keiichi Kojima ◽  
Shin Nakao ◽  
Susumu Yoshizawa ◽  
Shiho Kawanishi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proton Pump ◽  
Expression System ◽  
Spectroscopic Characterization ◽  
Functional Expression ◽  
Proton Pumping ◽  
E Coli ◽  
Oxyrrhis Marina ◽  
Domains Of Life

AbstractMicrobial rhodopsins are photoswitchable seven-transmembrane proteins that are widely distributed in three domains of life, archaea, bacteria and eukarya. Rhodopsins allow the transport of protons outwardly across the membrane and are indispensable for light-energy conversion in microorganisms. Archaeal and bacterial proton pump rhodopsins have been characterized using an Escherichia coli expression system because that enables the rapid production of large amounts of recombinant proteins, whereas no success has been reported for eukaryotic rhodopsins. Here, we report a phylogenetically distinct eukaryotic rhodopsin from the dinoflagellate Oxyrrhis marina (O. marina rhodopsin-2, OmR2) that can be expressed in E. coli cells. E. coli cells harboring the OmR2 gene showed an outward proton-pumping activity, indicating its functional expression. Spectroscopic characterization of the purified OmR2 protein revealed several features as follows: (1) an absorption maximum at 533 nm with all-trans retinal chromophore, (2) the possession of the deprotonated counterion (pKa = 3.0) of the protonated Schiff base and (3) a rapid photocycle through several distinct photointermediates. Those features are similar to those of known eukaryotic proton pump rhodopsins. Our successful characterization of OmR2 expressed in E. coli cells could build a basis for understanding and utilizing eukaryotic rhodopsins.

scholarly journals Hvordan kommer matematisk meningsskaping til syne i barns lek? En casestudie

2010 ◽  
Vol 3 (3) ◽  
Author(s):  
Vigdis Flottorp
Keyword(s):  
Day Care ◽  
Meaning Making ◽  
Communicative Competence ◽  
Field Work ◽  
Care Centre ◽  
Mathematical Meaning ◽  
Children's Play ◽  
Physical Context ◽  
Children’S Play ◽  
Day Care Centre

Title: Mathematical meaning making in children’s play? Verbal and non-verbal forms of expressionsAbstract: I analyze an episode from field work in a multilingual day-care centre in Oslo. I examine verbal and non-verbal expressions. The children are 5 years old, and the mathematics is about classification. The children are creating structure and are seeking meaning. This is a key part of their play. My findings indicate that mathematical order and structure become conscious experiences to the children. I argue that we cannot know about the children’s mathematical and communicative competence without knowing the physical context, the play in the sandpit, and the friendship between the boys.

scholarly journals Isolation and characterization of escherichia coli in ready-to-eat foods vended in Islamic University, Kushtia

1970 ◽  
Vol 18 ◽  
pp. 99-103 ◽  
Author(s):  
S Biswas ◽  
MAK Parvez ◽  
M Shafiquzzaman ◽  
S Nahar ◽  
MN Rahman
Keyword(s):  
Escherichia Coli ◽  
Pattern Analysis ◽  
University Campus ◽  
Rflp Pattern ◽  
E Coli ◽  
Fish Meat ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Identification And Characterization

Context: Escherichia coli is shed in the feces of warm blooded animals and humans and thus potential for public health. Detection and characterization of E. coli in the ready-to-eat (RTE) foods concerns due to their presence indicates fecal contamination of the food.   Objective: To identify, characterize and RFLP pattern analysis of E. coli isolated from RTE foods vended in Islamic University campus, Kushtia.   Materials and Methods: Fifty samples from four types of consumed foods in six student halls of residence, some temporary restaurants of Islamic University, Kushtia were assessed for bacterial contamination by standard methods. Identification and characterization of E. coli isolates were performed using IMViC tests. Genomic DNA was used to perform RFLP pattern analysis.   Results: Thirty seven out of 50 (74%) examined samples of RTE foods had E. coli contamination. The highest number of E. coli was isolated from vegetable oriented RTE foods (90.90%) and fish, meat and cereals samples were also significantly E. coli positive. RFLP profiling of two E. coli isolates were observed.   Conclusion: The results of this study provide evidence that some RTE foods had unsatisfactory levels of contamination with E. coli. Thus street vended RTE food could be important potential vehicles for food-borne diseases. Molecular characterization may be exploited to identify food borne pathogen among different species.  Keywords: Ready-to-eat foods; Escherichia coli; RFLP pattern DOI: http://dx.doi.org/10.3329/jbs.v18i0.8783 JBS 2010; 18(0): 99-103

scholarly journals Evaluation of antipyretic activity of hydroalcoholic extract of Corchorus depressus Linn. in Escherichia coli–induced pyretic rabbits

2018 ◽  
Vol 16 ◽  
pp. 205873921879295
Author(s):  
Saeed Ahmad ◽  
Muhammad Akram ◽  
Syed Muhammad Ali Shah ◽  
Sabira Sultana
Keyword(s):  
Escherichia Coli ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Hydroalcoholic Extract ◽  
Antipyretic Effect ◽  
E Coli ◽  
Antipyretic Activity ◽  
Experimental Treatments

This study was conducted to investigate the antipyretic effect of the hydroalcoholic extract of Corchorus depressus Linn. against Escherichia coli ( E. coli)-induced pyrexia in rabbits. Hydroalcohalic extracts of C. depressus were given orally at 25, 50, and 100 mg/kg for antipyretic affect in E. coli-induced fever in rabbits. The animals were divided into five groups of five each. Among these five groups, three received various doses of experimental treatments, whereas the fourth one served as positive control and received paracetamol. The fifth group of animals served as negative control and received no treatment. The body temperature of the rabbits was measured rectally over a period of 5 h. C. depressus exhibited better effects at dose rate of 25, 50, and 100 mg/kg. The hydroalcoholic extract of C. depressus has significant antipyretic effect. These results lend support to the popular use of C. depressus in traditional medicine as a remedy for pyrexia and suggest that the characterization of the principles for such activity deserves further investigation.

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