scholarly journals No Simple Relationship Between Dry Period and Early Lactation Responses of Plasma Non-Esterified Acids to in vivo b-Adrenergic Challenge in Holstein Friesian Cows

2003 ◽  
Vol 44 (Suppl 1) ◽  
pp. P122 ◽  
Author(s):  
P Theilgaard ◽  
NC Friggens ◽  
KH Sloth ◽  
KL Ingvartsen
1986 ◽  
Vol 43 (1) ◽  
pp. 7-15 ◽  
Author(s):  
I. M. Reid ◽  
C. J. Roberts ◽  
R. J. Treacher ◽  
L. A. Williams

ABSTRACTAn experiment was performed with two groups of nine British Friesian cows to compare the effect of calving in fat or thin condition on (1) the mobilization and functional activity of subcutaneous adipose tissue, (2) the mobilization of skeletal muscle, (3) the development and resolution of fatty infiltration of the liver and (4) the chemistry and haematology of blood. Sampling was performed at various times during the dry period and subsequent lactation. There were no differences between groups in the amount of adipose tissue mobilized between 4 weeks before and 26 weeks after calving. The lipogenic and lipolytic capacities of isolated adipocytes were also not different between groups at any time although major changes occurred in both over the calving period and during early lactation. Acetate oxidation to carbon dioxide was higher in adipocytes isolated from thin cows particularly after calving. More muscle fibre area was lost in the fat cows compared with the thin cows between 4 weeks before and 4 weeks after calving and the fat cows had greater infiltration of fat in the liver at 1 and 4 weeks after calving than the thin cows. The mean white-cell count was lower and the packed-cell volume was higher in the fat cows than in the thin cows at 1 week after calving. The major differences between groups in blood composition were increased concentrations of copper, non-esterified fatty acids, bilirubin and enzymes such as ornithine carbamyl transferase in the fat cows after calving. These results suggest that fat and thin cows respond differently to the metabolic demands of early lactation and that some of these differences render fat cows more susceptible to disease.


1998 ◽  
Vol 1998 ◽  
pp. 108-108
Author(s):  
J. A. Fregonesi ◽  
J.D. Leaver

Space allowance could be an important variable affecting production, health, reproductive performance and behaviour of dairy cattle. Also, high and low yielding cows may have different ways of coping with insufficient space allowance. The aim of this experiment was to study the influence of space allowance and milk yield level on the performance and behaviour of strawyard housed dairy cows.The experiment was carried out using 24 Holstein Friesian cows with two groups in early lactation of high (over 30 kg/day milk yield) and two groups in late lactation of low yield (under 25 kg/day milk yield). The groups were allocated to strawyard systems with low stocking density (bed area/cow = 9 m2; pen area/cow = 13.5 m2; feed face width/cow = 1.5 m) or high stocking density (bed area/cow = 4.5 m2; pen area/cow = 6.75 m2; feed face width/cow = 0.75m) conforming to a changeover design with two periods, each of four weeks. The cows were fed a total mixed ration ad libitum and 2kg/cow/day of concentrate in the milking parlour. All animals were milked twice daily.


animal ◽  
2016 ◽  
Vol 10 (5) ◽  
pp. 796-804 ◽  
Author(s):  
D. Lawrence ◽  
M. O’Donovan ◽  
T.M. Boland ◽  
E. Lewis ◽  
E. Kennedy

Author(s):  
K. Aston ◽  
W.J. Fisher ◽  
A.B. McAllan

Recent trials with cows fed grass silage have shown significant increases in intake and in yields of milk and milk solids when the crude protein (CP) concentration in a supplementary concentrate was raised. Giving additional CP in the concentrate was a more effective strategy for improving yields of milk and milk protein than giving extra energy (Aston et al 1992). The objective of this trial was to examine the influence of changes to the amount and pattern of distribution of CP supplied in a fixed concentrate ration given with grass silage ad libitum.Fifty-five Holstein-Friesian cows in their second and subsequent lactations were given a standard diet for two weeks from calving and then were used in a continuous feeding trial from weeks 4 to 21. The cows received 5 kg of fresh concentrate daily containing 156 (LP), 245 (MP) or 338 (HP) g CP per kg dry matter (DM), MP comprised equal amounts of LP and HP. Concentrate carbohydrate sources were cereals and digestible fibre and CP was increased by a mixture of 3:1 soya:fish meals. Grass silage contained 253 g toluene DM/kg, 162 g CP/kg DM, D value in vivo 0.723, pH 3.7, fermentation acids 135 g/kg DM of which 0.85 was lactic acid and NH3-N 91 g/kg total N.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
J. B. Garner ◽  
A. J. Chamberlain ◽  
C. Vander Jagt ◽  
T. T. T. Nguyen ◽  
B. A. Mason ◽  
...  

Abstract Heat stress in dairy cattle leads to reduction in feed intake and milk production as well as the induction of many physiological stress responses. The genes implicated in the response to heat stress in vivo are not well characterised. With the aim of identifying such genes, an experiment was conducted to perform differential gene expression in peripheral white blood cells and milk somatic cells in vivo in 6 Holstein Friesian cows in thermoneutral conditions and in 6 Holstein Friesian cows exposed to a short-term moderate heat challenge. RNA sequences from peripheral white blood cells and milk somatic cells were used to quantify full transcriptome gene expression. Genes commonly differentially expressed (DE) in both the peripheral white blood cells and in milk somatic cells were associated with the cellular stress response, apoptosis, oxidative stress and glucose metabolism. Genes DE in peripheral white blood cells of cows exposed to the heat challenge compared to the thermoneutral control were related to inflammation, lipid metabolism, carbohydrate metabolism and the cardiovascular system. Genes DE in milk somatic cells compared to the thermoneutral control were involved in the response to stress, thermoregulation and vasodilation. These findings provide new insights into the cellular adaptations induced during the response to short term moderate heat stress in dairy cattle and identify potential candidate genes (BDKRB1 and SNORA19) for future research.


1993 ◽  
Vol 73 (3) ◽  
pp. 547-557 ◽  
Author(s):  
H. Ben Salem ◽  
R. Krzeminski ◽  
A. Ferlay ◽  
M. Doreau

Four Holstein × Friesian cows with rumen and duodenal cannulae were assigned to a 4 × 4 Latin square design consisting of four experimental diets differing in the nature of forage and the presence or absence of rapeseed oil supplement. Dietary treatments were composed of 60% grassland hay and 40% production concentrate (diet H), and of 65% corn silage, 28% production concentrate and 7% soybean meal (diet S). Each diet was also supplied with 7% rapeseed oil. These supplemented diets are designated respectively HO and SO. Digestibility of organic matter was 69.0, 67.5, 73.6 and 70.0% for diets H, HO, S and SO, respectively. The differences were mainly due to variations in NDF digestibility: 55.0, 50.4, 56.9 and 46.9% for diets H, HO, S and SO, respectively. These results are consistent with variations in VFA concentration and composition. However, differences among diets in in situ cellulose degradation were lower than expected. Starch and nitrogen degradation measured in situ, and ruminal outflow rate of liquid and solids were not modified by lipid supply. Hydrogenation of linoleic and linolenic acids was high (mean 88.9 and 89.5%, respectively) and did not depend on the diet. Key words: Cow, digestion, rumen, lipid supply


1990 ◽  
Vol 30 (1) ◽  
pp. 7 ◽  
Author(s):  
SC Valentine ◽  
BD Bartsch

Milk production and composition was determined in Holstein-Friesian cows fed either 3.5 or 7.0 kg dry matter (DM) daily of lupin grain, pea grain, faba bean grain or barley grain with or without 1.5% added urea, as supplements to an oaten hay based diet. All the grains were hammermilled. Daily yields of milk (L), fat (kg) and protein (kg) were significantly (P<0.05) higher for cows fed lupin (20.0, 0.81, 0.57), pea (18.9, 0.80, 0.56) and faba bean (18.9, 0.79,0.55) grains compared with those of cows fed barley grain with (17.8, 0.73, 0.51) or without (18.0, 0.71, 0.52) urea. Yields of milk (L), fat (kg) and protein (kg) and milk protein content (g/kg) were significantly (P<0.05) higher when 7.0 kg DM (19.5, 0.80, 0.57, 29.6) compared with 3.5 kg DM (18.0,0.73,0.51,29.0) of grain was fed. There were no significant differences between treatments in hay DM intake by cows fed 3.5 kg DM of grain daily. A significantly (P<0.05) higher milk fat production per unit DM intake was recorded for cows fed legume compared with barley grain. There were generally higher returns above grain cost for cows fed legume compared with barley grain and for cows fed 4 kg compared to 8 kg daily of grain. It was concluded that it was more economical to feed 3.5 kg DM of legume grain compared with 3.5 kg DM of barley grain, with or without urea, as supplements for cows offered cereal hay in early lactation. However, the economics of feeding 7.0 kg DM of legume grain compared with 7.0 kg DM of barley grain and 7.0 kg DM compared with 3.5 kg DM of grain will depend on the costs of relative changes in hay intake associated with these practices.


2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Lisa Beuckelaere ◽  
Anneleen De Visscher ◽  
Fernando Nogueira Souza ◽  
Evelyne Meyer ◽  
Freddy Haesebrouck ◽  
...  

AbstractAlthough extensive research has been performed on bovine non-aureus staphylococci (NAS), several aspects such as bacteria-host interaction remain largely unstudied. Moreover, only a few mastitis pathogen challenge studies in cows have been conducted in the dry period, an important period that allows intramammary infection (IMI) to cure and new IMI to occur. We challenged 16 quarters of 4 Holstein Friesian cows at dry off with 100; 100 000 or 10 000 000 CFU of the udder-adapted S. chromogenes IM strain. Four quarters from one cow served as negative controls. Internally sealed quarters remained untouched, whereas non-sealed quarters were sampled 3 times during the dry period. After parturition, colostrum and daily milk samples were taken during the first week of lactation of all quarters. In total, 8 quarters appeared to be colonized, since S. chromogenes IM was recovered at least once during the experiment, as substantiated using Multilocus Sequence Typing. S. chromogenes IM shedding was highest in dry quarters inoculated with 10 000 000 CFU. Colonized quarters had the highest quarter somatic cell count (qSCC) in early lactation. Inoculated quarters (both colonized and non-colonized) had lower IL-6 and IL-10 concentrations in the dry period, whilst IFN-γ levels tended to be higher in colonized quarters compared to non-inoculated quarters. Also, IgG2 levels were higher in inoculated compared to non-inoculated quarters and the IgG2/IgG1 ratio was on average above 1. To conclude, we showed that dry quarters can be colonized with S. chromogenes IM, resulting in a shift towards a Th1 response in late gestation and early lactation characterised by an increased IgG2 concentration. However, further research is needed to confirm our findings.


1996 ◽  
Vol 1996 ◽  
pp. 85-85 ◽  
Author(s):  
R.J. Dewhurst ◽  
D.W.R. Davies ◽  
W.J. Fisher ◽  
K. Aston

Previous studies (Moorby et al, 1994) have shown increased yields of protein and lactose in the subsequent lactation as a consequence of feeding additional undegradable dietary protein (UDP) and restricting energy intake during the dry period. This experiment was undertaken to investigate the effect of supplementary UDP in the dry period diet of Holstein-Friesian cows given unrestricted access to grass silage and 1.5 kg of barley per day.Sixty-one cows received diets based on ad libitum access to grass silage with either (a) 1.5 kg barley / day or (b) 1.0 kg barley and 0.5 kg Maize Gluten Meal / day. After calving, cows had ad libitum access to grass silage (Metabolisable Energy (ME)=11.3 MJ/kg DM; crude protein (CP)=181 g/kg DM) and received 5 kg/day of concentrates (ME=13.0 MJ/kg DM; CP=250g/kg DM).


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