Biodistribution of gold nanoparticles in mouse lung following intratracheal instillation

ABSTRACT Nonsurgical intratracheal instillation of 1 μg of purified, recombinant flagellin in several strains of mice stimulated a transient innate immune response in the lung characterized by the infiltration of neutrophils and the rapid production of tumor necrosis factor alpha, interleukin 6, granulocyte colony-stimulating factor, and the chemokines keratinocyte-derived chemokine, MIP1α, and MIP-2.

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A method for intratracheal instillation of endotoxin into the lungs of mice

Laboratory Animals ◽

10.1258/002367789780810536 ◽

1989 ◽

Vol 23 (3) ◽

pp. 234-240 ◽

Cited By ~ 31

Author(s):

B. Starcher ◽

I. Williams

Keyword(s):

Cell Population ◽

Neutrophil Count ◽

Intratracheal Instillation ◽

Mouse Lung ◽

Equal Distribution ◽

Detailed Method ◽

Low Levels

A detailed method is presented for the intratracheal instillation of fluids into the lungs of mice. Using 125I-albumen it was determined that 25-50 µl of volume gave almost equal distribution to all lobes of the mouse lung. Extremely low levels of endotoxin given intratracheally (12·5-25 ng) resulted in a dramatic increase in the neutrophil content of the lung. The increase started in less than 4 h, reaching levels of 7 × 105 by 18 h and was maintained at elevated levels for at least 48 h. This neutrophil count represented more than 90% of the cell population of the lung.

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Biodistribution of PEG-modified gold nanoparticles following intratracheal instillation and intravenous injection

Biomaterials ◽

10.1016/j.biomaterials.2010.05.009 ◽

2010 ◽

Vol 31 (25) ◽

pp. 6574-6581 ◽

Cited By ~ 347

Author(s):

Jens Lipka ◽

Manuela Semmler-Behnke ◽

Ralph A. Sperling ◽

Alexander Wenk ◽

Shinji Takenaka ◽

...

Keyword(s):

Gold Nanoparticles ◽

Intravenous Injection ◽

Intratracheal Instillation

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Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00210.2007 ◽

2008 ◽

Vol 295 (2) ◽

pp. L314-L325 ◽

Cited By ~ 27

Author(s):

R. A. Bem ◽

A. W. Farnand ◽

V. Wong ◽

A. Koski ◽

M. E. Rosenfeld ◽

...

Keyword(s):

Epithelial Cells ◽

Lung Injury ◽

Alveolar Macrophages ◽

Fas Ligand ◽

Intratracheal Instillation ◽

Alveolar Epithelial Cells ◽

Mouse Lung ◽

Epithelial Cell Line ◽

Alveolar Epithelial

Activation of the Fas/Fas ligand (FasL) system in the lungs results in a form of injury characterized by alveolar epithelial apoptosis and neutrophilic inflammation. Studies in vitro show that Fas activation induces apoptosis in alveolar epithelial cells and cytokine production in alveolar macrophages. The main goal of this study was to determine the contribution of alveolar macrophages to Fas-induced lung inflammation in mice, by depleting alveolar macrophages using clodronate-containing liposomes. Liposomes containing clodronate or PBS were instilled by intratracheal instillation. After 24 h, the mice received intratracheal instillations of the Fas-activating monoclonal antibody Jo2 or an isotype control antibody and were studied 18 h later. The Jo2 MAb induced increases in bronchoalveolar lavage fluid (BALF) total neutrophils, lung caspase-3 activity, and BALF total protein and worsened histological lung injury in the macrophage-depleted mice. Studies in vitro showed that Fas activation induced the release of the cytokine KC in a mouse lung epithelial cell line, MLE-12. These results suggest that the lung inflammatory response to Fas activation is not primarily dependent on resident alveolar macrophages and may instead depend on cytokine release by alveolar epithelial cells.

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Formation of an oxidative DNA damage, 8-hydroxydeoxyguanosine, in mouse lung DNA after intratracheal instillation of diesel exhaust particles and effects of high dietary fat and beta-carotene on this process

Carcinogenesis ◽

10.1093/carcin/16.6.1441 ◽

1995 ◽

Vol 16 (6) ◽

pp. 1441-1445 ◽

Cited By ~ 73

Author(s):

Makoto Nagashima ◽

Hiroshi Kasai ◽

Jun Yokota ◽

Yukio Nagamachi ◽

Takamichi Ichinose ◽

...

Keyword(s):

Dna Damage ◽

Dietary Fat ◽

Diesel Exhaust ◽

Oxidative Dna Damage ◽

Intratracheal Instillation ◽

Beta Carotene ◽

Diesel Exhaust Particles ◽

Mouse Lung ◽

Exhaust Particles

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Reactivity of Pulmonary Alveolar Cells to Crocidolite Asbestos Fibers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054285 ◽

1982 ◽

Vol 40 ◽

pp. 334-335

Author(s):

Charles L. Sanders ◽

Roy R. Adee

Keyword(s):

Osmium Tetroxide ◽

Intratracheal Instillation ◽

Uranyl Acetate ◽

Fiber Suspension ◽

Nacl Solution ◽

Alveolar Cells ◽

Asbestos Fibers ◽

Fischer Rats ◽

Mineral Silicates

Asbestos is a generic name for a group of hydrated mineral silicates that occur naturally in a fibrous form. The early interactions of asbestos fibers with alveolar cells in large part determines their long-term toxicity. Young adult, SPF, Fischer rats were given a single intratracheal instillation of 2 mg crocidolite asbestos suspended in 0.5 ml of 0.9% NaCl solution. About 80% of the fibers had lengths of less than 10 ym as measured on light micrographs of the fiber suspension. Two rats were killed at 3 hr, 1 d and 1, 4, 8, 12 and 16 wk after instillation and the lungs instilled with 8 ml McDowell - Trumps at 20 cm H2O. Lung tissue was dehydrated and sputtered coated with palladium-gold for SEM or post-fixed in osmium tetroxide, embedded in epoxy resin and sections stained with uranyl acetate and lead citrate for TEM.

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Ultrastructure of rat alveolar macrophages activated in situ by poly:IC

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128158 ◽

1987 ◽

Vol 45 ◽

pp. 768-769

Author(s):

A. M. Klinkner ◽

R. A. Weiss ◽

A. Kelley ◽

P. J. Bugelski

Keyword(s):

Venous Blood ◽

Intratracheal Instillation ◽

Buffy Coat ◽

Fischer 344 Rats ◽

Blood Monocytes ◽

Fischer 344 ◽

Polyinosinic:Polycytidylic Acid ◽

Macrophage Activator ◽

Endogenous Peroxidase

Polyinosinic:polycytidylic acid is an inducer of interferon and a macrophage activator. We have found that intratracheal instillation of polyI:C (IT-pI:C) activates rat bronchoalveolar lavage cells (BAL) for a variety of functions. Examination of Giemsa stained, cytocentrifuge preparations showed that IT-pI:C induced a population of BAL not seen in resident BAL. The morphology of these cells suggested that they might be derived from blood monocytes. To test this hypothesis we have examined several populations of macrophages that had been stained for endogenous peroxidase activity as a marker of cells derived from the monocyte-macrophage lineage.Macrophages were obtained from Fischer 344 rats. Peritoneal exudate cells (PEC) were collected by lavage 4 days after i.p. injection of 20 ml 3% thioglycolate. Buffy coat monocytes were separated from venous blood from naive rats.

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