scholarly journals Effect of different concentrations of growth regulator on callus induction of the rice lineage AB11047

2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
Natália Segatto ◽  
Martina Bianca Fuhrmann ◽  
Tatiane Casarin ◽  
Carla Ferreira Silveira ◽  
Luciana Bicca Dode ◽  
...  
Keyword(s):  
Growth Regulator ◽  
Callus Induction

scholarly journals Organogenesis Kelapa Sawit (Elaeis guineensis Jacq.) Asal Eksplan Bunga Betina

2016 ◽  
Vol 2 (2) ◽  
pp. 104
Author(s):  
Tyas Larasati ◽  
Suci Rahayu ◽  
Fauziyah Harahap
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Callus Induction ◽  
Plant Growth Regulator ◽  
Elaeis Guineensis ◽  
Female Flower ◽  
Dry Weight ◽  
Flower Plant ◽  
Elaeis Guineensis Jacq ◽  
Dichlorophenoxy Acetic Acid

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D.   Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis

scholarly journals Plant Regeneration from Protocorm-derived Callus of Five Paphiopedilum Hybrids

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1101D-1101
Author(s):  
Michael Compton
Keyword(s):  
Plant Regeneration ◽  
Growth Regulator ◽  
Callus Induction ◽  
Callus Formation ◽  
Induction Medium ◽  
Regeneration Medium ◽  
Petri Dishes ◽  
Callus Induction Medium

Callus was induced from protocorms of five Paphiopedilum hybrids (Paph. 03-1, Paph. 03-4, Paph. 03-5, Paph. 03-6, and Paph. 03-7) on callus induction medium [MS inorganics (412.5 mg NH4NO3 instead of 1650 mg and 475 mg KNO3 instead of 1900 mg) and vitamins plus (per liter) 0.1 g myo-inositol, 30 g sucrose, and 2.5 g Gelrite; pH 5.5] containing various concentrations and combinations of thidiazuron (TDZ; 4.5 and 45 μm) and 2,4-D (4.5 and 45 μm). Callus formation was greatest for protocorms of Paph. 03-1, Paph. 03-4, Paph. 03-6, and Paph. 03-7. Among the most competent hybrids, callus formation was greatest among protocorms induced in medium containing 4.5 μm 2,4-D and 4.5 to 45 μm TDZ. Induced calli were transferred to 100 × 15 mm petri dishes containing 25 mL of PLB and plant regeneration medium (similar to callus induction medium) containing various concentrations of either benzyladenine (BA; 0.5, 5, or 10 μm), TDZ (0.25, 2.5, or 5 μm) or no growth regulator (control). PLB and plant formation was greatest on medium containing BA.

scholarly journals PENGARUH 2,4- D TERHADAP PENINGKATAN KUALITAS DAN KUANTITAS KALUS YANG DIINDUKSI DENGAN KOMBINASI AUKSIN DAN SITOKININ PADA KULTUR DAUN RUMPUT BENGGALA ( Panicum maximum) VARIETAS TRICHOGLUME

2021 ◽  
Vol 9 (1) ◽  
pp. 27-35
Author(s):  
Marna Eoh
Keyword(s):  
Acetic Acid ◽  
Growth Regulator ◽  
Callus Induction ◽  
Panicum Maximum ◽  
Standard Medium ◽  
Leaf Culture ◽  
Friable Callus ◽  
Brown Colour ◽  
Callus Production

To determine the effect various concentration 2, 4, 6 and 8 mg/l of 2,4- D (2,4- dicloropenoxy acetic acid) on callus induction of Benggala grass variety Trichoglume leaf culture on Murashige and Skoog (MS) standard medium and its organogenesis stimulated using deffent concentration of growth regulator, namely [N6- (2- isopenteny)- adenine] or 2iP (0, 0.2 and 1.0 mg/l ) and (l- naphthalene acetic ent, wacid) or NAA (0, 0.03, 0.16 and 3.0 mg/l) were performed. Percentage of callus were measured and organogenesis from callus were subjected to description analysis . The results showed that callus induction was optimum when 2,4-D was treated at 4 mg/l, friable callus were produced. Percentage production of callus week 4 was 46.2 percent, while using 8 mg/l 2,4 –D the callus production was about 59.7 percent yellowish coloured and more compact callus were produced. Combination of 0 mg/l 2iP ( auxin) +  3mg/l   (cytokinin) at 3 weeks showed reseilted 100 % of calluses produced roots, the highest amaunt roots (9,0) was observed in combination 0 mg/l 2iP + 3 mg/l NAA, and the longest root (17,0 mm) was recorded in combination 0 mg/l 2iP + 3 mg/l NAA. Calluses yielded  varied from white, yellowish to brown colour.

scholarly journals Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

2014 ◽  
Vol 24 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maryam Vahedi ◽  
Siamak Kalantari ◽  
Seyed Alireza Salami
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulator ◽  
Callus Induction ◽  
Growth Parameters ◽  
Crocus Sativus ◽  
Factors Affecting ◽  
Callus Production ◽  
Planting Materials ◽  
Disease Free ◽  
Crocus Sativus L

Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA.  In some treatments, calli were transferred to organogenesis stage after two subcultures.  For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ  proved to be the best growth regulator treatment for shoot regeneration from the saffron calli. Plant Tissue Cult. & Biotech. 24(1): 1-9, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19184

scholarly journals Plant regeneration through somatic embryogenesis in two cultigroups of Pineapple (Ananas comosus L.)

2021 ◽  
Author(s):  
Gilles H.T. Cacaï ◽  
Brunith A.M. Ahokpossi ◽  
Serge S. Houédjissin ◽  
Jerome Anani Houngue ◽  
Bienvenue T. Badou ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Significant Influence ◽  
Growth Regulator ◽  
Callus Induction ◽  
Somatic Embryos ◽  
High Performance ◽  
High Rate ◽  
Ananas Comosus ◽  
Base Medium

Abstract BackgroundPineapple production is mostly constrained by unavailability of high-performance suckers. However, somatic embryogenesis (SE) have been revealed the most rapid and controllable method for Pineapple propagation than conventional sucker production methods. The aim of this study was to evaluate the responses of two cultivars of pineapple regenerated through somatic embryogenesis. MethodsThus, calli were induced from crown leaf and plantlets leaf of Smooth Cayenne and Sugar Loaf cultivars. Murashige and Skoog base medium supplemented with vitamins B5 and different in hormonal combinations: Auxins / Cytokins. BAP and / or 2,4-D have been added to base medium for calli maturation and BAP and GA3 for plant regeneration. ResultsThe results indicated a significant influence of type of explant and copper on callus induction in pineapple cultivars. Likewise, The medium MS with growth regulator combination NAA (0.5 mg/l) + BAP (1mg / l (BAP) has a highly significant influence with 8.8 mature somatic embryos. Also, the growth regulator combinations and the cultivars have significantly influenced somatic embryos regeneration with a high rate of 55.25% shoots by using the hormonal combination BAP (3 mg/l) + GA3 (2 mg/l) for the smooth Cayenne. Conclusion Leaves from organogenesis plantlets constitute the explants to be used for callus induction in pineapple. The combination of BAP (1 mg/l) + copper (2 mg/l) + Picloram (6 mg/l or 12 mg/l) on Murashige and Skoog medium supplemented with vitamins B5 was favorable somatic embryos regeneration of pineapple. The protocol developed is a key study for successful mass propagation and genetics transformation of pineapple.

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