The Influence of Various Growth Regulator of Growth Media on Biomass and Callus Induction in Elephantopus scaber Linn

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D. Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis

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Effect of Carbon Sources on Cell Growth and Regeneration Ability in Three Cultivars of Banana

Journal of Bio-Science ◽

10.3329/jbs.v17i0.7111 ◽

1970 ◽

Vol 17 ◽

pp. 83-88

Author(s):

MJ Hossain ◽

MA Bari ◽

NA Ara ◽

SM Shahinul Islam

Keyword(s):

Cell Growth ◽

Callus Induction ◽

Carbon Sources ◽

Optimal Dose ◽

Vital Role ◽

Growth Media ◽

Regeneration Ability ◽

Comparative Performance ◽

Media Formulation ◽

Male Flowers

Context: Carbon plays a vital role in plant cell growth and regeneration in artificial media but the source of carbon deserves scientific investigation to analysis their comparative performance. Objectives: To analyze the comparative performance of different carbon sources (glucose, sucrose and sorbitol) in cell growth and regeneration efficiency of banana (Musa spp) cultivars. Materials and Methods: Male flowers of banana cultivars cv. Sabri, Gine and Ranginsagar were used in this experiment. Male flowers were cut into small pieces and they were transferred in petri dishes containing Murashige and Skoog media supplemented with 2 mg/l 2,4-D + 1mg/l NAA + 1mg/l IAA + 1mg/l Biotin + 1mg/l glutamine and 3% (w/v) different sugars: sucrose, glucose, and sorbitol singly or in combinations autoclaved in 121ºC temperature for 20 min. The pH of the medium was adjusted to 5.8. Results: Glucose showed the highest performance in callus induction and cell growth and 3% glucose proved as the optimal dose in media formulation for callus induction and cell growth. Sucrose and sorbitol behaves differently in embryo formation and they produced the highest and lowest number of embryos respectively in regeneration medium. In respect of overall performance the highest percentages of shoot and root formation was obtained in the media containing 3% sucrose. Conclusion: Glucose proved to be the best carbon source in callus induction and cell growth media. Key words: Banana; Musa; callus; single cell; regenerationDOI: 10.3329/jbs.v17i0.7111J. bio-sci. 17: 83-88, 2009

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Plant Regeneration from Protocorm-derived Callus of Five Paphiopedilum Hybrids

HortScience ◽

10.21273/hortsci.40.4.1101d ◽

2005 ◽

Vol 40 (4) ◽

pp. 1101D-1101

Author(s):

Michael Compton

Keyword(s):

Plant Regeneration ◽

Growth Regulator ◽

Callus Induction ◽

Callus Formation ◽

Induction Medium ◽

Regeneration Medium ◽

Petri Dishes ◽

Callus Induction Medium

Callus was induced from protocorms of five Paphiopedilum hybrids (Paph. 03-1, Paph. 03-4, Paph. 03-5, Paph. 03-6, and Paph. 03-7) on callus induction medium [MS inorganics (412.5 mg NH4NO3 instead of 1650 mg and 475 mg KNO3 instead of 1900 mg) and vitamins plus (per liter) 0.1 g myo-inositol, 30 g sucrose, and 2.5 g Gelrite; pH 5.5] containing various concentrations and combinations of thidiazuron (TDZ; 4.5 and 45 μm) and 2,4-D (4.5 and 45 μm). Callus formation was greatest for protocorms of Paph. 03-1, Paph. 03-4, Paph. 03-6, and Paph. 03-7. Among the most competent hybrids, callus formation was greatest among protocorms induced in medium containing 4.5 μm 2,4-D and 4.5 to 45 μm TDZ. Induced calli were transferred to 100 × 15 mm petri dishes containing 25 mL of PLB and plant regeneration medium (similar to callus induction medium) containing various concentrations of either benzyladenine (BA; 0.5, 5, or 10 μm), TDZ (0.25, 2.5, or 5 μm) or no growth regulator (control). PLB and plant formation was greatest on medium containing BA.

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In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha45210899 ◽

2017 ◽

Vol 45 (2) ◽

pp. 392-399

Author(s):

Dragana M. SKOČAJIĆ ◽

Marija M. NEŠIĆ ◽

Marina Ž. NONIĆ ◽

Milica M. FOTIRIĆ AKŠIĆ ◽

Mihailo N. GRBIĆ ◽

...

Keyword(s):

Plant Growth ◽

Callus Induction ◽

Prunus Avium ◽

Growth Media ◽

Cherry Tree ◽

Adult Tissues ◽

Flowering Cherry ◽

Cherry Rootstocks ◽

Cherry Trees

Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)-compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata ‘Amanogawa’, ‘Kanzan’ and ‘Kiku-shidare-zakura’) and two domestic cherry rootstocks – Prunus avium and Prunus ‘Colt’. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars ‘Kanzan’ and ‘Colt’, the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ‘Colt’ and Prunus serrulata ‘Kanzan’, whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.

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In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

Proceedings of the Mathematics, Informatics, Science, and Education International Conference (MISEIC 2019) ◽

10.2991/miseic-19.2019.3 ◽

2019 ◽

Author(s):

Yuliani ◽

Fida Rachmadiarti ◽

Sari Kusuma Dewi ◽

Mahanani Tri Asri

Keyword(s):

In Vitro Propagation ◽

Growth Media ◽

Elephantopus Scaber ◽

Culture Growth

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PENGARUH 2,4- D TERHADAP PENINGKATAN KUALITAS DAN KUANTITAS KALUS YANG DIINDUKSI DENGAN KOMBINASI AUKSIN DAN SITOKININ PADA KULTUR DAUN RUMPUT BENGGALA ( Panicum maximum) VARIETAS TRICHOGLUME

Agrinimal Jurnal Ilmu Ternak dan Tanaman ◽

10.30598/ajitt.2021.9.1.27-35 ◽

2021 ◽

Vol 9 (1) ◽

pp. 27-35

Author(s):

Marna Eoh

Keyword(s):

Acetic Acid ◽

Growth Regulator ◽

Callus Induction ◽

Panicum Maximum ◽

Standard Medium ◽

Leaf Culture ◽

Friable Callus ◽

Brown Colour ◽

Callus Production

To determine the effect various concentration 2, 4, 6 and 8 mg/l of 2,4- D (2,4- dicloropenoxy acetic acid) on callus induction of Benggala grass variety Trichoglume leaf culture on Murashige and Skoog (MS) standard medium and its organogenesis stimulated using deffent concentration of growth regulator, namely [N6- (2- isopenteny)- adenine] or 2iP (0, 0.2 and 1.0 mg/l ) and (l- naphthalene acetic ent, wacid) or NAA (0, 0.03, 0.16 and 3.0 mg/l) were performed. Percentage of callus were measured and organogenesis from callus were subjected to description analysis . The results showed that callus induction was optimum when 2,4-D was treated at 4 mg/l, friable callus were produced. Percentage production of callus week 4 was 46.2 percent, while using 8 mg/l 2,4 â€“D the callus production was about 59.7 percent yellowish coloured and more compact callus were produced. Combination of 0 mg/l 2iP ( auxin) + 3mg/l (cytokinin) at 3 weeks showed reseilted 100 % of calluses produced roots, the highest amaunt roots (9,0) was observed in combination 0 mg/l 2iP + 3 mg/l NAA, and the longest root (17,0 mm) was recorded in combination 0 mg/l 2iP + 3 mg/l NAA. Calluses yielded varied from white, yellowish to brown colour.

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Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v24i1.19184 ◽

2014 ◽

Vol 24 (1) ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Maryam Vahedi ◽

Siamak Kalantari ◽

Seyed Alireza Salami

Keyword(s):

Shoot Regeneration ◽

Growth Regulator ◽

Callus Induction ◽

Growth Parameters ◽

Crocus Sativus ◽

Factors Affecting ◽

Callus Production ◽

Planting Materials ◽

Disease Free ◽

Crocus Sativus L

Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA. In some treatments, calli were transferred to organogenesis stage after two subcultures. For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ proved to be the best growth regulator treatment for shoot regeneration from the saffron calli. Plant Tissue Cult. & Biotech. 24(1): 1-9, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19184

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