Relationship and prognostic significance of SPARC and VEGF protein expression in colon cancer

Purpose The significance of low microsatellite instability (MSI-L) in colorectal cancer is poorly understood. No clear biologic distinction has been found between MSI-L and microsatellite stable (MSS) colorectal cancer, and these two phenotypes are usually combined when analyzed against the well-defined high MSI (MSI-H) phenotype. Evidence is emerging that an O6-methylguanine DNA methyltransferase (MGMT) gene defect is associated with MSI-L. Therefore, to further define this phenotype, we undertook a detailed analysis of the prognostic significance of MSI-L and loss of MGMT expression in colon cancer. Patients and Methods The study cohort was 183 patients with clinicopathologic stage C colon cancer who had not received adjuvant therapy. We analyzed MSI status, MGMT, and mismatch repair protein expression, as well as MGMT and p16 promoter hypermethylation. Results We showed that MSI-L defines a group of patients with poorer survival (P = .026) than MSS patients, and that MSI-L was an independent prognostic indicator (P = .005) in stage C colon cancer. Loss of MGMT protein expression was associated with the MSI-L phenotype but was not a prognostic factor for overall survival in colon cancer. p16 methylation was significantly less frequent in MSI-L than in MSI-H and MSS tumors and was not associated with survival. Conclusion MSI-L characterizes a distinct subgroup of stage C colon cancer patients, including the MSI-L subset of proximal colon cancer, who have a poorer outcome. Neither the MGMT defect nor p16 methylation are likely to contribute to the worse prognosis of the MSI-L phenotype.

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S100A4 May Be a Good Prognostic Marker and a Therapeutic Target for Colon Cancer

Journal of Oncology ◽

10.1155/2018/1828791 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Sabahattin Destek ◽

Vahit Onur Gul

Keyword(s):

Colon Cancer ◽

Protein Expression ◽

Tumor Progression ◽

Tumor Tissue ◽

Patient Survival ◽

S100 Protein ◽

Prognostic Significance ◽

Tnm Staging ◽

Expression Levels ◽

S100a4 Protein

Background. Globally, the colorectal cancers rank the third in terms of cancer incidence and rank the fourth in cancer-associated deaths. S100A4, an important member of the S100 protein family, serves to promote tumor progression and metastasis. By conducting this study, we aim to examine the role of S100A4 in the prognosis of colon cancer and to demonstrate its prognostic significance. Methods. Tissue samples of colon cancer from 148 patients who underwent colon resection due to colon cancer were analyzed by immunohistochemical staining to determine the protein expression levels of S100A4. The protein expression levels of S100A4 in tumor tissue were matched with the clinicopathologic factors including patient survival. Results. Cytoplasmic expression of S100A4 protein was demonstrated in the tumor tissue of 132 patients (89.2%) out of a total of 148 study patients. Statistically, the expression levels of the cytoplasmic S100A4 protein correlated significantly with the TNM stages and patient survival. The distribution of the S100A4 protein staining in the tumor tissue was associated with the age groups, tumor localization, TNM staging, and patient survival with statistical significance. The levels of S100A4 protein expression were found to be an independent prognostic factor for TNM staging and poor survival. Conclusion. Expression of the S100A4 protein in colon cancers may be an indicator of tumor progression and lymph node metastasis and may be useful for predicting the overall survival of the patients with colon cancer. In patients with colon cancer, it may be used as an indicator of poor prognosis.

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Abstract 1190: Prognostic significance of serine synthesis pathway-related protein expression in patients with resected colon cancer

10.1158/1538-7445.am2015-1190 ◽

2015 ◽

Author(s):

Byung Woog Kang ◽

Jong Gwang Kim ◽

Yee Soo Chae ◽

Soo Jung Lee ◽

Shinkyo Yoon ◽

...

Keyword(s):

Colon Cancer ◽

Protein Expression ◽

Prognostic Significance ◽

Related Protein

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Human Endogenous Retroviruses Long Terminal Repeat Methylation, Transcription, and Protein Expression in Human Colon Cancer

Frontiers in Oncology ◽

10.3389/fonc.2020.569015 ◽

2020 ◽

Vol 10 ◽

Author(s):

Maria Dolci ◽

Chiara Favero ◽

Wafa Toumi ◽

Evaldo Favi ◽

Letizia Tarantini ◽

...

Keyword(s):

Colon Cancer ◽

Protein Expression ◽

Long Terminal Repeat ◽

Human Colon ◽

Endogenous Retroviruses ◽

Terminal Repeat ◽

Human Colon Cancer ◽

Human Endogenous Retroviruses

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Prognostic significance of bcl-2 protein expression in aggressive non- Hodgkin's lymphoma. Groupe d'Etude des Lymphomes de l'Adulte (GELA)

Blood ◽

10.1182/blood.v87.1.265.265 ◽

1996 ◽

Vol 87 (1) ◽

pp. 265-272 ◽

Cited By ~ 332

Author(s):

O Hermine ◽

C Haioun ◽

E Lepage ◽

MF d'Agay ◽

J Briere ◽

...

Keyword(s):

Overall Survival ◽

Protein Expression ◽

B Cell ◽

Hodgkin’S Lymphoma ◽

Prognostic Significance ◽

Hodgkin's Lymphoma ◽

Independent Effect ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma ◽

Large B Cell

Abstract Little is known about the expression of bcl-2 protein in intermediate and high grade non-Hodgkin's lymphoma (NHL) and its clinical and prognostic significance. We performed immunohistochemical analysis of bcl-2 expression in tumoral tissue sections of 348 patients with high or intermediate grade NHL. These patients were uniformly treated with adriamycin, cyclophosphamide, vindesine, bleomycin, and prednisone (ACVBP) in the induction phase of the LNH87 protocol. Fifty eight cases were excluded due to inadequate staining. Of the 290 remaining patients, 131 (45%) disclosed homogeneous positivity (high bcl-2 expression) in virtually all tumor cells, whereas 65 (23%) were negative and 94 (32%) exhibited intermediate staining. High bcl-2 expression was more frequent in B-cell NHL (109 of 214, 51%) than in T- cell NHL (6 of 35, 17%) (P = .0004), and was heterogeneously distributed among the different histological subtypes. Further analysis was performed on the 151 patients with diffuse large B-cell lymphoma (centroblastic and immunoblastic) to assess the clinical significance and potential prognostic value of bcl-2 expression in the most frequent and homogeneous immunohistological subgroup. High bcl-2 expression, found in 44% of these patients (67 of 151), was more frequently associated with III-IV stage disease (P = .002). Reduced disease-free survival (DFS) (P < .01) and overall survival (P < .05) were demonstrated in the patients with high bcl-2 expression. Indeed, the 3-year estimates of DFS and overall survival were 60% and 61%, respectively (high bcl-2 expression) versus 82% and 78%, respectively (negative/intermediate bcl-2 expression). A multivariate regression analysis confirmed the independent effect of bcl-2 protein expression on DFS. Thus bcl-2 protein expression, as demonstrated in routinely paraffin-embedded tissue, appears to be predictive of poor DFS, in agreement with the role of bcl-2 in chemotherapy-induced apoptosis. It might be considered as a new independent biologic prognostic parameter, which, especially in diffuse large B-cell NHL, could aid in the identification of patient risk groups.

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Prognostic significance of K- ras and TP53 mutations in the role of adjuvant chemotherapy on survival in patients with dukes C colon cancer

Diseases of the Colon & Rectum ◽

10.1007/bf02234733 ◽

2001 ◽

Vol 44 (3) ◽

pp. 358-363 ◽

Cited By ~ 18

Author(s):

W. A. Bleeker ◽

V. M. Hayes ◽

A. Karrenbeld ◽

R. M. W. Hofstra ◽

E. Verlind ◽

...

Keyword(s):

Colon Cancer ◽

Adjuvant Chemotherapy ◽

Prognostic Significance ◽

Tp53 Mutations ◽

Dukes C

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Prognostic Significance and Correlation with Survival of bcl-2 and TGF-β RII in Colon Cancer

Digestive Diseases and Sciences ◽

10.1023/b:ddas.0000007864.24866.77 ◽

2003 ◽

Vol 48 (12) ◽

pp. 2284-2289 ◽

Cited By ~ 12

Author(s):

Gregory Kouraklis ◽

John Kakisis ◽

Stamatios Theoharis ◽

Antonia Tzonou ◽

Andromachi Glinavou ◽

...

Keyword(s):

Colon Cancer ◽

Prognostic Significance

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Prognostic impact of β-2-microglobulin expression in colorectal cancers stratified by mismatch repair status

Journal of Clinical Pathology ◽

10.1136/jclinpath-2012-200742 ◽

2012 ◽

Vol 65 (11) ◽

pp. 996-1002 ◽

Cited By ~ 21

Author(s):

Viktor Hendrik Koelzer ◽

Kristi Baker ◽

Daniela Kassahn ◽

Daniel Baumhoer ◽

Inti Zlobec

Keyword(s):

Protein Expression ◽

Mismatch Repair ◽

Immune Cell ◽

Prognostic Significance ◽

Clinicopathological Features ◽

Prognostic Impact ◽

Mismatch Repair Status ◽

First Time ◽

Intra Class Correlation Coefficient

Backgroundβ-2-microglobulin (B2M) is essential for antigen presentation, yet may also possess proto-oncogenic properties.AimTo determine the prognostic impact of B2M in patients with mismatch repair (MMR) proficient and deficient colorectal cancer (CRC) and to investigate whether this effect on outcome is dependent on the local immune response. MethodsB2M protein expression and tumour-infiltrating immune cells (CD3, CD16, CD163, CD20, CD4, CD45RO, CD56, CD68, CD8, FoxP3, GranzymeB, iNOS, mast cell tryptase, MUM1, PD1, TIA-1) were evaluated in a well characterised tissue microarray of 408 CRCs. The predictive value for clinicopathological features and the prognostic significance of B2M expression were analysed, stratified by MMR status and the immunohistological characteristics of immune cell infiltrates. ResultsInterobserver agreement for B2M staining was high (intra-class correlation coefficient=0.91). Complete B2M loss was more frequent in MMR-deficient (19.4%) compared to MMR-proficient (7.1%) tumours (p<0.001). In MMR-deficient cases, B2M loss predicted rare local recurrence (p=0.034), infrequent nodal-positivity (p=0.035), absence of distant metastasis (p=0.048; sensitivity=100%) and a trend towards favourable survival (p=0.124) independent of immune infiltrates. No associations between B2M and clinicopathological features were observed in MMR-proficient cases.ConclusionsOur data show for the first time that absence of B2M protein expression identifies MMR-deficient cancers with a favourable clinical course and absence of metastatic disease. Validation of B2M protein expression for sub-classification of MMR-deficient CRC is recommended for future clinical trials.

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Prognostic Significance of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand and Its Receptors in Adjuvantly Treated Stage III Colon Cancer Patients

Oncology Times ◽

10.1097/01.cot.0000265627.84452.ab ◽

2007 ◽

Vol 29 (1) ◽

pp. 26

Author(s):

Caroline M. van Geelen ◽

Jantine L. Westra ◽

Elisabeth G. de Vries

Keyword(s):

Colon Cancer ◽

Tumor Necrosis Factor ◽

Cancer Patients ◽

Tumor Necrosis ◽

Prognostic Significance ◽

Stage Iii ◽

Stage Iii Colon Cancer ◽

Necrosis Factor

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Proliferating cell nuclear antigen (PCNA), p53 and MDM2 expression in Hodgkin’s disease

Sao Paulo Medical Journal ◽

10.1590/s1516-31802007000200003 ◽

2007 ◽

Vol 125 (2) ◽

pp. 77-84 ◽

Cited By ~ 4

Author(s):

Gevina Silva Pinheiro ◽

Maria Regina Régis Silva ◽

Celso Arrais Rodrigues ◽

José Kerbauy ◽

José Salvador Rodrigues de Oliveira

Keyword(s):

Protein Expression ◽

Hodgkin's Disease ◽

Proliferating Cell Nuclear Antigen ◽

Hodgkin’S Disease ◽

Prognostic Significance ◽

Sao Paulo ◽

Nuclear Antigen ◽

São Paulo ◽

Proliferation Markers ◽

Proliferating Cell

CONTEXT AND OBJECTIVE: Tumor cells in Hodgkin’s disease (HD) express cell proliferation markers that are evaluated according to the oncogenes involved or the expression of their proteins. Correlations between the protein expression grade and clinical data are now important for disease prognosis. DESIGN AND SETTING: This was a retrospective analysis on proliferating cell nuclear antigen (PCNA), p53 and MDM2 (murine double minute-2) expression using immunohistochemistry, on formalin-fixed, paraffin-embedded tissues from diagnostic biopsies on 51 patients with HD. The study was conducted at the Division of Hematology and Transfusion Medicine, Hospital São Paulo, Universidade Federal de São Paulo. METHODS: Antigen expression was evaluated as the proportions of positive Hodgkin and Reed-Sternberg (HRS) cells and reactive lymphocytes (L), which were compared using Spearman correlation coefficients. The Friedman test was used for comparisons between the markers. The Pearson test was used to investigate associations between marker expression and clinical and laboratory parameters, marrow involvement, complete remission (CR) and overall survival (OS) rates. RESULTS: There was overexpression of antigen proteins in HRS, in relation to L (p < 0.001). In HRS, MDM2 was higher than p53 and PCNA (p < 0.003), while the latter two were equivalent. In L, p53 was lower than MDM2 and PCNA (p < 0.001), while the latter two were equivalent. There was no relationship between protein expression and clinical and laboratory variables or outcome. CONCLUSIONS: PCNA, p53 and MDM2 are tumor markers for HD, but showed no clinical or prognostic significance in our analysis.

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