Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

Download Full-text

A combination of mild chemical pre-treatment and enzymatic hydrolysis efficiently produces xylooligosaccharides from sugarcane bagasse

Journal of Cleaner Production ◽

10.1016/j.jclepro.2021.125972 ◽

2021 ◽

Vol 291 ◽

pp. 125972

Author(s):

Shuai Zhao ◽

Gui-Ling Zhang ◽

Chen Chen ◽

Qi Yang ◽

Xue-Mei Luo ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Pre Treatment

Download Full-text

The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse

BioEnergy Research ◽

10.1007/s12155-021-10294-0 ◽

2021 ◽

Author(s):

Leidy Patricia Quintero ◽

Nathalia P. Q. de Souza ◽

Adriane M. F. Milagres

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

High Solid ◽

Hydrolysis Of

Download Full-text

Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽

10.3390/biom11040507 ◽

2021 ◽

Vol 11 (4) ◽

pp. 507

Author(s):

Eduardo Troncoso-Ortega ◽

Rosario del P. Castillo ◽

Pablo Reyes-Contreras ◽

Patricia Castaño-Rivera ◽

Regis Teixeira Mendonça ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Eucalyptus Globulus ◽

Steam Explosion ◽

Laser Scanning ◽

Structural Changes ◽

Micro Particles ◽

Cellulose Accessibility ◽

Ft Ir ◽

Enzymatic Accessibility ◽

Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

Download Full-text

Enhanced enzymatic hydrolysis and hydrogen production of sugarcane bagasse pretreated by peroxyformic acid

Bioresource Technology ◽

10.1016/j.biortech.2021.124751 ◽

2021 ◽

Vol 326 ◽

pp. 124751

Author(s):

Jie Bu ◽

Yu-Tao Wang ◽

Mao-Cheng Deng ◽

Ming-Jun Zhu

Keyword(s):

Enzymatic Hydrolysis ◽

Hydrogen Production ◽

Sugarcane Bagasse ◽

Peroxyformic Acid

Download Full-text

A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01884-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Lin Dai ◽

Tian Huang ◽

Kankan Jiang ◽

Xin Zhou ◽

Yong Xu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Production Cost ◽

Solid Loading ◽

Fed Batch ◽

Acid Pretreatment ◽

Furoic Acid ◽

Cooling Crystallization ◽

Mineral Acids ◽

Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

Download Full-text