scholarly journals Dual activity of Serratia marcescens Pt-3 in phosphate-solubilizing and production of antifungal volatiles

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Andong Gong ◽  
Gaozhan Wang ◽  
Yake Sun ◽  
Mengge Song ◽  
Cheelo Dimuna ◽  
...  
Keyword(s):  
Plant Growth ◽  
Serratia Marcescens ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Plant Pathogens ◽  
Solid Phase ◽  
Dimethyl Disulfide ◽  
Control Activity ◽  
Multiple Functions

Abstract Background Soil fertility decline and pathogen infection are severe issues for crop production all over the world. Microbes as inherent factors in soil were effective in alleviating fertility decrease, promoting plant growth and controlling plant pathogens et al. Thus, screening microbes with fertility improving and pathogen controlling properties is of great importance to humans. Results Bacteria Pt-3 isolated from tea rhizosphere showed multiple functions in solubilizing insoluble phosphate, promoting plant growth, producing abundant volatile organic compounds (VOCs) and inhibiting the growth of important fungal pathogens in vitro. According to the 16S rRNA phylogenetic and biochemical analysis, Pt-3 was identified to be Serratia marcescens. The solubilizing zone of Pt-3 in the medium of lecithin and Ca3(PO4)2 was 2.1 cm and 1.8 cm respectively. In liquid medium and soil, the concentration of soluble phosphorus reached 343.9 mg.L− 1, and 3.98 mg.kg− 1, and significantly promoted the growth of maize seedling, respectively. Moreover, Pt-3 produced abundant volatiles and greatly inhibited the growth of seven important phytopathogens. The inhibition rate ranged from 75.51 to 100% respectively. Solid phase micro-extraction coupled with gas chromatography tandem mass spectrometry proved that the antifungal volatile was dimethyl disulfide. Dimethyl disulfide can inhibit the germination of Aspergillus flavus, and severely destroy the cell structures under scanning electron microscopy. Conclusions S. marcescens Pt-3 with multiple functions will provide novel agent for the production of bioactive fertilizer with P-solubilizing and fungal pathogens control activity.

scholarly journals Dual Activity of Serratia Marcescens Pt-3 in Phosphate-solubilizing and Production of Antifungal Volatiles

2021 ◽  
Author(s):  
Andong Gong ◽  
Gaozhan Wang ◽  
Mengge Song ◽  
Cheelo Dimuna ◽  
Zhen Gao ◽  
...  
Keyword(s):  
Plant Growth ◽  
Serratia Marcescens ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Plant Pathogens ◽  
Solid Phase ◽  
Dimethyl Disulfide ◽  
Control Activity ◽  
Multiple Functions

Abstract Background: Soil fertility decline and pathogen infection are severe issues for crop production all over the world. Microbes as inherent factors in soil were effective in alleviating fertility decrease, promoting plant growth and controlling plant pathogens et al. Thus, screening microbes with fertility improving and pathogen controlling are great importance to humans.Results: Bacteria Pt-3 isolated from tea rhizosphere showed multiple functions in solubilizing insoluble phosphate, promoting plant growth, producing abundant volatile organic compounds (VOCs) and inhibiting the growth of important fungal pathogens in vitro. According to the 16S rRNA phylogenetic and biochemical analysis, Pt-3 was identified to be Serratia marcescens. The solubilizing zone of Pt-3 in the medium of lecithin and Ca3(PO4)2 was 2.1 cm and 1.8 cm respectively. In liquid medium and soil, the concentration of soluble phosphorus reached 343.9 mg.L-1, and 3.98 mg.kg-1, and significantly promoted the growth of maize seedling, respectively. Moreover, Pt-3 produced abundant volatiles and greatly inhibited the growth of seven important phytopathogens. The inhibition rate ranged from 75.51 to 100% respectively. Solid phase micro-extraction coupled with gas chromatography tandem mass spectrometry proved that the antifungal volatile was dimethyl disulfide. Dimethyl disulfide can inhibit the germination of Aspergillus flavus, and severely destroy the cell structures under scanning electron microscopy.Conclusions: S. marcescens Pt-3 with multiple functions will provide novel agent for the production of bioactive fertilizer with P-solubilizing and fungal pathogens control activity.

scholarly journals The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 349
Author(s):  
Dominik Bleša ◽  
Pavel Matušinský ◽  
Romana Sedmíková ◽  
Milan Baláž
Keyword(s):  
Biological Control ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Higher Plants ◽  
Fusarium Culmorum ◽  
Suppressive Effect ◽  
Plant Production ◽  
Shoot Biomass ◽  
Organic Substrates

The use of biological control is becoming a common practice in plant production. One overlooked group of organisms potentially suitable for biological control are Rhizoctonia-like (Rh-like) fungi. Some of them are capable of forming endophytic associations with a large group of higher plants as well as mycorrhizal symbioses. Various benefits of endophytic associations were proved, including amelioration of devastating effects of pathogens such as Fusarium culmorum. The advantage of Rh-like endophytes over strictly biotrophic mycorrhizal organisms is the possibility of their cultivation on organic substrates, which makes their use more suitable for production. We focused on abilities of five Rh-like fungi isolated from orchid mycorrhizas, endophytic fungi Serendipita indica, Microdochium bolleyi and pathogenic Ceratobasidium cereale to inhibit the growth of pathogenic F. culmorum or Pyrenophora teres in vitro. We also analysed their suppressive effect on wheat infection by F. culmorum in a growth chamber, as well as an effect on barley under field conditions. Some of the Rh-like fungi affected the growth of plant pathogens in vitro, then the interaction with plants was tested. Beneficial effect was especially noted in the pot experiments, where wheat plants were negatively influenced by F. culmorum. Inoculation with S. indica caused higher dry shoot biomass in comparison to plants treated with fungicide. Prospective for future work are the effects of these endophytes on plant signalling pathways, factors affecting the level of colonization and surviving of infectious particles.

scholarly journals Potential for Plant Growth Promotion of Rhizobacteria Associated withSalicorniaGrowing in Tunisian Hypersaline Soils

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Francesca Mapelli ◽  
Ramona Marasco ◽  
Eleonora Rolli ◽  
Marta Barbato ◽  
Hanene Cherif ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Crop Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Growth Promotion ◽  
Arid Ecosystems ◽  
Acetic Acid Production ◽  
Halotolerant Bacteria ◽  
Microbiome Composition

Soil salinity and drought are among the environmental stresses that most severely affect plant growth and production around the world. In this study the rhizospheres ofSalicorniaplants and bulk soils were collected fromSebkhetandChotthypersaline ecosystems in Tunisia. Depiction of bacterial microbiome composition by Denaturing Gradient Gel Electrophoresis unveiled the occurrence of a high bacterial diversity associated withSalicorniaroot system. A large collection of 475 halophilic and halotolerant bacteria was established fromSalicorniarhizosphere and the surrounding bulk soil, and the bacteria were characterized for the resistance to temperature, osmotic and saline stresses, and plant growth promotion (PGP) features. TwentyHalomonasstrains showed resistance to a wide set of abiotic stresses and were able to perform different PGP activitiesin vitroat 5% NaCl, including ammonia and indole-3-acetic acid production, phosphate solubilisation, and potential nitrogen fixation. By using agfp-labelled strain it was possible to demonstrate thatHalomonasis capable of successfully colonisingSalicorniaroots in the laboratory conditions. Our results indicated that the culturable halophilic/halotolerant bacteria inhabiting salty and arid ecosystems have a potential to contribute to promoting plant growth under the harsh salinity and drought conditions. These halophilic/halotolerant strains could be exploited in biofertilizer formulates to sustain crop production in degraded and arid lands.

Morphological and molecular based identification of Antifungal bacillus licheniformis

2017 ◽  
Vol 17 (1) ◽  
pp. 31-35
Author(s):  
B Oyuntogtokh ◽  
M Byambasuren
Keyword(s):  
Bacillus Licheniformis ◽  
Fungal Pathogens ◽  
Crop Production ◽  
Plant Pathogens ◽  
Control Plant ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Bacterial Strains ◽  
Fusarium Spp ◽  
Bacterial Cultures

At present, plant diseases caused by soil borne plant pathogens have major constraints on crop production. Which include genera Fusarium spp, Phytophtora spp, Sclerotinia and Altenaria. Due to this reason, chemical fungicides are routinely used to control plant disease, which is also true in Mongolian case. However, use of these chemicals has caused various problems including environmental pollution with consequence of toxicity to human health also resistance of some pathogens to these fungicides are present. Fortunately, an alternative method to reduce the effect of these plant pathogens is the use of antagonist microorganisms. Therefore, some species of the genus Bacillus are recognized as one of the most effective biological control agent.Our research was focused to isolate Bacillus licheniformis, with antifungal potential, from indigenous sources. In the current study, 28 bacterial cultures were isolated from soil and fermented mare’s milk also named as koumiss. Isolated bacterial cultures were identified according to simplified key for the tentative identification of typical strain of Bacillus species. As a result 8 strains were positive and further screened for antifungal activity against Fusarium spp and Alternaria solani. Out of these 8 strains 5 strains are selected based on their high effectiveness against fungal pathogens and for further confirmation Polymerase Chain reaction run for effective bacterial strains using specific primers B.Lich-f and B.Lich-r. 

Control of Postharvest Fungal Pathogens by Antifungal Compounds from Penicillium expansum

2013 ◽  
Vol 76 (11) ◽  
pp. 1879-1886 ◽  
Author(s):  
WAFA ROUISSI ◽  
LUISA UGOLINI ◽  
CAMILLA MARTINI ◽  
LUCA LAZZERI ◽  
MARTA MARI
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Fungal Pathogens ◽  
Solid Phase ◽  
Petri Dish ◽  
Pathogenic Fungi ◽  
Penicillium Expansum ◽  
Colletotrichum Acutatum ◽  
Volatile Organic

The fungicidal effects of secondary metabolites produced by a strain of Penicillium expansum (R82) in culture filtrate and in a double petri dish assay were tested against one isolate each of Botrytis cinerea, Colletotrichum acutatum, and Monilinia laxa and six isolates of P. expansum, revealing inhibitory activity against every pathogen tested. The characterization of volatile organic compounds released by the R82 strain was performed by solid-phase microextraction–gas chromatographic techniques, and several compounds were detected, one of them identified as phenethyl alcohol (PEA). Synthetic PEA, tested in vitro on fungal pathogens, showed strong inhibition at a concentration of 1,230 μg/ml of airspace, and mycelium appeared more sensitive than conidia; nevertheless, at the concentration naturally emitted by the fungus (0.726 ± 0.16 μg/ml), commercial PEA did not show any antifungal activity. Therefore, a combined effect between different volatile organic compounds produced collectively by R82 can be hypothesized. This aspect suggests further investigation into the possibility of exploiting R82 as a nonchemical alternative in the control of some plant pathogenic fungi.

scholarly journals Comparison of Two Commercial Recirculated Aquacultural Systems and Their Microbial Potential in Plant Disease Suppression

2021 ◽  
Author(s):  
Sammar Khalil ◽  
Preeti Panda ◽  
Farideh Ghadamgahi ◽  
AnnaKarin Rosberg ◽  
Ramesh R Vetukuri
Keyword(s):  
Plant Growth ◽  
Fish Species ◽  
Plant Pathogens ◽  
Extracellular Enzymes ◽  
Production Systems ◽  
Sampling Site ◽  
Disease Suppression ◽  
Bacterial Strains ◽  
Promising Tool

Aquaponics are food production systems advocated for food security and health. Their sustainability from a nutritional and plant health perspective is, however, a significant challenge. Recirculated aquaculture systems (RAS) form a major part of aquaponic systems but knowledge about their potential to benefit plant growth and suppress their pathogens is limited. The current study tested if the diversity and function of microbial communities in two commercial RAS were specific to the fish species used (Tilapia or Clarias) and sampling site (fish tanks and wastewaters), and whether they confer benefits to plants and have in vitro antagonistic potential towards plant pathogens. Microbial diversity and composition was found to be dependent on fish species and sample site. The Tilapia RAS hosted higher bacterial diversity than the Clarias RAS; but the latter hosted higher fungal diversity. Both Tilapia and Clarias RAS hosted bacterial and fungal communities that promoted plant growth, inhibited plant pathogens and encouraged biodegradation. The production of extracellular enzymes, related to nutrient availability and pathogen control, by bacterial strains isolated from the Tilapia and Clarias systems, makes them a promising tool in aquaponics and in their system design.

scholarly journals Importance of plant growth promoting rhizobacteria (PGPR) in agriculture: A Review

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Tabish Akhtar ◽  
Shubham Kumar ◽  
Sukhdeo Kumar ◽  
M. R. Meena
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Plant Pathogens ◽  
Crop Yields ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agricultural Industry ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Increasing Demand ◽  
Environmentally Sound

The growth of plants promoting rhizobacteria (PGPR) has gained widespread importance in agriculture. These are beneficial bacteria found in nature that live actively in plant roots and improve plant growth and increase agricultural productivity.. (PGPR) promoting plant growth shows an important role in the sustainable agricultural industry. The increasing demand for crop production is a major challenge nowadays, with a significant lack of use of synthetic chemical fertilizers and pesticides. The use of PGPR has proven to be an environmentally sound way of increasing crop yields by facilitating plant growth through direct or indirect mechanisms. The mechanisms of PGPR include regulating hormonal and nutritional balance, inducing resistance against plant pathogens, and dissolving nutrients for easy uptake by plants. Furthermore, PGPRs show synergistic and antagonistic interactions with microorganisms within the rhizosphere and in bulk soils, which indirectly increases plant growth rates. There are several bacteria species that act as PGPR. This review summarizes the methodology of PGPR as a bio-fertilizer in agriculture.

scholarly journals Antifungal activity in vitro of Sapranthus microcarpus (Annonaceae) against phytopathogens

2018 ◽  
Author(s):  
Iván De-la-Cruz-Chacón ◽  
Nora Yazmin López-Fernández ◽  
Christian Anabí Riley-Saldaña ◽  
Marisol Castro Moreno ◽  
Alma Rosa González-Esquinca
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Curvularia Lunata ◽  
Root Bark ◽  
Inhibitory Activities ◽  
Alkaloid Extracts

Background and Aims: The phytochemistry study of Annonaceae has intensified in the last decades due to the discovery of secondary metabolites with antimicrobial activities. The inhibitory activities of extracts and compounds from these species for phytopathogens are less known. The antifungal activity of Sapranthus microcarpus was determined in vitro against six fungal pathogens of important crops.Methods: Hexane, methanol, and alkaloid extracts of the leaves and root and stem bark of S. microcarpus were evaluated to determine whether they could inhibit the mycelial growth of the phytopathogens. The combined activity of the most active extracts for each phytopathogen was also determined. The alkaloid liriodenine was isolated and identified as an antifungal principle.Key results: All extracts inhibited the growth of the six phytopathogens to some extent. The root bark alkaloid extract showed the highest activity. Fusarium oxysporum f. sp. lycopersici and Curvularia lunata were the most sensitive phytopathogens. Liriodenine was active against all the plant pathogens (minimum inhibitory concentrations of 125-500 nmol ml-1).Conclusions: Sapranthus microcarpus is a native natural resource with great phytochemical potential.

scholarly journals Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana

2020 ◽  
Vol 6 (4) ◽  
pp. 341
Author(s):  
Nongnat Phoka ◽  
Nakarin Suwannarach ◽  
Saisamorn Lumyong ◽  
Shin-ichi Ito ◽  
Kenji Matsui ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Antifungal Activity ◽  
Plant Growth ◽  
Fungal Pathogens ◽  
Solid Phase ◽  
Defense Responses ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Host ◽  
Trichoderma Species ◽  
Biocontrol Potential

Fungal volatile organic compounds (VOCs) emitted by Trichoderma species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by Trichoderma asperelloides PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in Arabidopsis thaliana. The strain’s VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92–84.95% in a volatile antifungal bioassay. The VOCs of T. asperelloides PSU-P1 promoted the plant growth of A. thaliana, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated A. thaliana relative to those of the control. High expression levels of the chitinase (CHI) and β-1,3-glucanase (GLU) genes were found in the VOC-treated A. thaliana by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated A. thaliana had higher defense-related enzyme (peroxidase (POD)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography–mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that T. asperelloides PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in A. thaliana.

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