Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat

The Dwarf53 (D53) gene, first studied in rice, encodes a protein that acts as a repressor of the physiological response of plants to strigolactones—substances that regulate the activity of axillary buds, stem growth, branching of roots and other physiological processes. In this work, we isolated and sequenced the homolog of the D53 gene in several accessions of the wild grass Dasypyrum villosum of different geographical origins, resulting in the discovery of large allelic variety. A molecular marker was also created that allows us to differentiate the D. villosum D53 gene from common wheat genes. Using this marker and monosomic addition, substitution and translocation wheat lines carrying the known D. villosum chromosomes, the D53 gene was localized on the long arm of the 5V chromosome.

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An update of the Courtot × Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat

Theoretical and Applied Genetics ◽

10.1007/s00122-002-1044-8 ◽

2003 ◽

Vol 106 (3) ◽

pp. 530-538 ◽

Cited By ~ 171

Author(s):

P. Sourdille ◽

T. Cadalen ◽

H. Guyomarc'h ◽

J. Snape ◽

M. Perretant ◽

...

Keyword(s):

Molecular Marker ◽

Linkage Map ◽

Chinese Spring ◽

Agronomic Traits ◽

Qtl Detection

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Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits

Field Crops Research ◽

10.1016/j.fcr.2013.10.007 ◽

2014 ◽

Vol 156 ◽

pp. 22-29 ◽

Cited By ~ 24

Author(s):

Bachir Goudia Daoura ◽

Liang Chen ◽

Yingying Du ◽

Yin-Gang Hu

Keyword(s):

Triticum Aestivum ◽

Common Wheat ◽

Qtl Analysis ◽

Agronomic Traits ◽

Triticum Aestivum L ◽

Genetic Effects ◽

Dwarfing Gene

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Application Techniques of Molecular Marker and Achievement of Marker Assisted Selection (MAS) in Three Major Crops Rice, Wheat and Maize

International Journal for Research in Applied Sciences and Biotechnology ◽

10.31033/ijrasb.8.1.10 ◽

2021 ◽

Vol 8 (1) ◽

pp. 82-93

Author(s):

Muhammad Gul Arabzai ◽

Hameed Gul

Keyword(s):

Molecular Markers ◽

Molecular Marker ◽

Abiotic Stresses ◽

Marker Assisted Selection ◽

Agronomic Traits ◽

Linkage Drag ◽

Conventional Breeding ◽

Phenotype Data ◽

Application Techniques ◽

New Varieties

With the discovery of new genetic technology, the researcher focuses on using DNA molecular markers to improve new varieties worldwide. Such as resistance to biotic and abiotic stresses and enhancing quality and quantity at different plant breeding fields. Conventional breeding selection is based on phenotype data selection, time-consuming, and has a high chance of linkage drag. Thus, DNA molecular marker method usage is faster, easy, and not expensive than conventional breeding programs. This review focused on applying molecular markers such as genetic diversity analysis, the genotype of identification and fingerprinting, gene tagging and mapping, QTL analysis, and marker-assisted selection. In another part of this review, we focused on MAS's achievements related to improving agronomic traits, quality traits, and biotic/abiotic stresses for three major cereal crops like Wheat, Rice, and Maize.

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Molecular marker assisted gene stacking for disease resistance and quality genes in the dwarf mutant of an elite common wheat cultivar Xiaoyan22

10.21203/rs.2.12047/v2 ◽

2019 ◽

Author(s):

weijun zheng ◽

Song Li ◽

ZiHui Liu ◽

Qi Zhou ◽

Yanru Feng ◽

...

Keyword(s):

Disease Resistance ◽

Molecular Marker ◽

Resistance Gene ◽

Agronomic Traits ◽

Wheat Cultivar ◽

Yellow Rust ◽

Field Evaluation ◽

Wheat Breeding ◽

Dwarf Mutant ◽

Gene Stacking

Abstract Development of wheat cultivars with multiple disease resistance and high quality are major objectives in modern wheat breeding programs. Gene stacking is an efficient approach to achieve this target. In this study, we pyramided yellow rust resistance gene (Yr26), powdery mildew resistance gene (ML91260) and high-molecular-weight glutenin subunits Dx5+Dy10 into the dwarf mutant of an elite wheat cultivar, Xiaoyan22. Six pyramided lines were obtained by molecular marker-assisted selection (MAS) and field evaluation of disease resistance. The desirable agronomic traits of pyramided lines, their identity with the original cultivar Xiaoyan22 except for plant height, tiller number and disease resistance, was achieved in this study. Meanwhile, the yield of pyramided lines is higher than Xiaoyan22 in the field test. In addition, analysis of flour quality indicated that the dough stability time of pyramided lines was longer than that of Xiaoyan22. These results demonstrate that it is feasible to improve multiple agronomic traits simultaneously by rational application of MAS.

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Agronomic traits and grain quality of selected spelt wheat varieties versus common wheat

Journal of Crop Improvement ◽

10.1080/15427528.2020.1761921 ◽

2020 ◽

Vol 34 (5) ◽

pp. 654-675

Author(s):

Karolina Ratajczak ◽

Hanna Sulewska ◽

Szymańska Grażyna ◽

Przemysław Matysik

Keyword(s):

Common Wheat ◽

Grain Quality ◽

Agronomic Traits ◽

Wheat Varieties ◽

Spelt Wheat

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Elite Haplotypes of a Protein Kinase Gene TaSnRK2.3 Associated with Important Agronomic Traits in Common Wheat

Frontiers in Plant Science ◽

10.3389/fpls.2017.00368 ◽

2017 ◽

Vol 08 ◽

Cited By ~ 11

Author(s):

Lili Miao ◽

Xinguo Mao ◽

Jingyi Wang ◽

Zicheng Liu ◽

Bin Zhang ◽

...

Keyword(s):

Protein Kinase ◽

Common Wheat ◽

Agronomic Traits ◽

Kinase Gene ◽

Protein Kinase Gene

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Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

International Journal of Molecular Sciences ◽

10.3390/ijms21051861 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1861

Author(s):

Yajuan Wang ◽

Deyu Long ◽

Yanzhen Wang ◽

Changyou Wang ◽

Xinlun Liu ◽

...

Keyword(s):

In Situ Hybridization ◽

Powdery Mildew ◽

Common Wheat ◽

Agronomic Traits ◽

Genomic In Situ Hybridization ◽

Substitution Line ◽

Aegilops Geniculata ◽

The Common ◽

Sequential Fish

Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae. geniculata alien disomic substitution line W16998 was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST–STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization–genomic in situ hybridization (sequential FISH–GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST–STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic–tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH–GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7Mg. Therefore, W16998 was a wheat–Ae. geniculata 7Mg (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding.

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