scholarly journals Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Kyung-A Hwang ◽  
Yu-Jin Hwang ◽  
Jin Song
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Inhibitory Effects ◽  
Cornus Officinalis ◽  
Ethanol Extracts ◽  
And Oxidative Stress

scholarly journals Huangkui Capsule Attenuates Lipopolysaccharide-Induced Acute Lung Injury and Macrophage Activation by Suppressing Inflammation and Oxidative Stress in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jinfang Deng ◽  
Zhenpeng He ◽  
Xiuru Li ◽  
Wei Chen ◽  
Ziwen Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Macrophage Activation ◽  
Neutrophil Infiltration ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Tnf Α ◽  
And Oxidative Stress

Background. Huangkui capsule (HKC) comprises the total flavonoid extract of flowers of Abelmoschus manihot (L.) Medicus. This study aimed to explore the effects of HKC on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and LPS-stimulated RAW 264.7 cells. Methods. Enzyme-linked immunosorbent assay, histopathology, spectrophotometry, and quantitative real-time polymerase chain reaction were used for the assessments. Statistical analysis was performed using a one-way analysis of variance. Results. LPS significantly increased lung inflammation, neutrophil infiltration, and oxidative stress and downregulated lung miR-451 expression. Treatment with HKC dramatically attenuated the lung wet/dry weight ratio, reduced the total cell count in the bronchoalveolar lavage fluid (BALF), and inhibited myeloperoxidase activity in the lung tissues 24 h after LPS challenge. Histopathological analysis demonstrated that HKC attenuated LPS-induced tissue oedema and neutrophil infiltration in the lung tissues. Additionally, the concentrations of tumour necrosis factor- (TNF-) α and interleukin- (IL-) 6 in BALF and IL-6 in the plasma reduced after HKC administration. Moreover, HKC could enhance glutathione peroxidase and catalase activities and upregulate the expression of miR-451 in the lung tissues. In vitro experiments revealed that HKC inhibited the production of nitric oxide, TNF-α, and IL-6 in LPS-induced RAW 264.7 cells and mouse primary peritoneal macrophages. Additionally, HKC downregulated LPS-induced transcription of TNF-α and IL-6 in RAW 264.7 cells. Conclusions. These findings suggest that HKC has anti-inflammatory and antioxidative effects that may protect mice against LPS-induced ALI and macrophage activation.

scholarly journals Maggot Extracts Alleviate Inflammation and Oxidative Stress in Acute Experimental Colitis via the Activation of Nrf2

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Rong Wang ◽  
Yongzheng Luo ◽  
Yadong Lu ◽  
Daojuan Wang ◽  
Tingyu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Colitis ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Related Factor ◽  
Protective Effects ◽  
Protein Levels ◽  
Bowel Diseases ◽  
Effective Therapeutic Strategy ◽  
And Oxidative Stress

Ulcerative colitis (UC) is a common chronic remitting disease driven through altered immune responses with production of inflammatory cytokines. Oxidant/antioxidant balance is also suggested to be an important factor for the recurrence and progression of UC. Maggots are known as a traditional Chinese medicine also known as “wu gu chong.” NF-E2-related factor-2 (Nrf2) transcription factor regulates the oxidative stress response and also represses inflammation. The aim of this study was to investigate the effects of maggot extracts on the amelioration of inflammation and oxidative stress in a mouse model of dextran sulfate sodium- (DSS-) induced colitis and evaluate if the maggot extracts could repress inflammation and oxidative stress using RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). In the present study, we found that the maggot extracts significantly prevented the loss of body weight and shortening of colon length in UC induced by DSS. Furthermore, DSS-induced expression of proinflammatory cytokines at both mRNA and protein levels in the colon was also attenuated by the maggot extracts. In addition, the maggot extracts could significantly suppress the expression of interleukin- (IL-) 1β, IL-6, TNF-α, NFκB p65, p-IκB, p22-phox, and gp91-phox in LPS-stimulated RAW 264.7 cells and colonic tissues. The maggot extracts increased the level of Nrf2 and prevented the degradation of Nrf2 through downregulating the expression of Keap1, which resulted in augmented levels of HO-1, SOD, and GSH-Px and reduced levels of MPO and MDA. However, after administering an Nrf2 inhibitor (ML385) to block the Nrf2/HO-1 pathway, we failed to observe the protective effects of the maggot extracts in mice with colitis and RAW 264.7 cells. Taken together, our data for the first time confirmed that the maggot extracts ameliorated inflammation and oxidative stress in experimental colitis via modulation of the Nrf2/HO-1 pathway. This study sheds light on the possible development of an effective therapeutic strategy for inflammatory bowel diseases.

scholarly journals Antioxidative Effects of Thymus quinquecostatus CELAK through Mitochondrial Biogenesis Improvement in RAW 264.7 Macrophages

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 548
Author(s):  
Jin Young Hong ◽  
Hyunseong Kim ◽  
Wan-Jin Jeon ◽  
Seungho Baek ◽  
In-Hyuk Ha
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Biogenesis ◽  
Antioxidant Activities ◽  
Cell Damage ◽  
Raw 264.7 ◽  
Mrna Levels ◽  
Dot Blot ◽  
Raw 264.7 Macrophages ◽  
Antioxidative Effects ◽  
And Oxidative Stress

Oxidative stress plays a key role in the pathogenesis of several diseases, including neurodegenerative diseases. Recent studies have reported that mitochondrial dysfunction is a leading cause of the overproduction of reactive oxygen species and oxidative stress. Mitochondrial changes play an important role in preventing oxidative stress. However, there is a lack of experimental evidence supporting this hypothesis. Thymus quinquecostatus CELAK (TQC) extract is a plant from China belonging to the thymus species, which can mediate the inflammatory response and prevent cell damage through its antioxidant activities. This study examines whether TQC can scavenge excess ROS originating from the mitochondria in RAW 264.7 macrophages. We used lipopolysaccharide (LPS) to induce inflammation and oxidative stress in RAW 264.7 macrophages and performed an immunocytochemistry dot blot of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and real-time PCR to analyze the expression levels of genes involved in mitochondrial biogenesis and oxidative metabolism. TQC was found to significantly reduce the intensity of immunostained MitoSOX and 8-OHdG levels in the total genomic DNA within the mitochondria in RAW 264.7 macrophages. The HO-1 and Nrf2 mRNA levels were also significantly increased in the TQC groups. Therefore, we verified that TQC improves mitochondrial function and attenuates oxidative stress induced by LPS. Our results can provide reference for the effect of TQC to develop new therapeutic strategies for various diseases.

scholarly journals Astaxanthin Inhibits Alcohol-induced Inflammation and Oxidative Stress in Macrophages (P02-014-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Hyunju Kang ◽  
Yoojin Lee ◽  
Minkyung Bae ◽  
Myung Joo Han ◽  
Young-Ki Park ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Bone Marrow ◽  
Raw 264.7 ◽  
Inhibitory Effects ◽  
Macrophage Cell ◽  
Excessive Alcohol Consumption ◽  
Antioxidant Genes ◽  
Raw 264.7 Macrophages ◽  
Ros Accumulation ◽  
And Oxidative Stress

Abstract Objectives Macrophages play crucial roles in the development of alcohol-induced inflammation. We previously demonstrated the inhibitory effects of astaxanthin (ASTX), a xanthophyll carotenoid, on the expression of pro-inflammatory and antioxidant genes as well as cellular accumulation of reactive oxygen species (ROS) in macrophages stimulated with lipopolysaccharides. The objective of this study was to determine whether ASTX can prevent alcohol-induced inflammation and oxidative stress in macrophages with the elucidation of the underlying mechanisms. Methods RAW 264.7 macrophages, a murine macrophage cell line, and mouse bone marrow-derived macrophages (BMDM) were treated with 80 mM ethanol in the absence or presence of 25 mM of ASTX for 72 h with daily media change. The expression levels of pro-inflammatory and antioxidant genes and proteins were measured by quantitative real-time PCR and Western blot, respectively. Cellular ROS accumulation was also determined in both macrophage cell types. Results ASTX significantly decreased ethanol-induced mRNA expression of interleukin (IL)-6, IL-1β, and tumor necrosis factor α (TNFα) as well as TNFα secretion in RAW 264.7 macrophages. Elevated levels of cellular ROS levels by ethanol were also attenuated by ASTX with a concomitant decrease in the expression of NADPH oxidase 2, a ROS-producing enzyme, in RAW 264.7 macrophages. The similar inhibitory effects of ASTX on inflammatory gene expression and cellular ROS accumulation were observed with BMDM. Furthermore, ethanol significantly decreased in sirtuin1 (SIRT1) mRNA and protein levels, which were markedly attenuated by ASTX in RAW 264.7 macrophages. Conclusions ASTX inhibited the expression and production of pro-inflammatory mediators and ROS accumulation in both RAW 264.7 macrophages and BMDM when the cells were exposed to ethanol. The anti-inflammatory effect of ASTX in ethanol-treated macrophages may be mediated, at least in part, by the activation of the SIRT1 pathway. Thus, ASTX may be used for the prevention of inflammatory and oxidative conditions triggered by excessive alcohol consumption. Funding Sources This study was supported by USDA Multistate Hatch.

scholarly journals Multiple Biological Activities of Rhododendron przewalskii Maxim. Extracts and UPLC-ESI-Q-TOF/MS Characterization of Their Phytochemical Composition

2021 ◽  
Vol 12 ◽  
Author(s):  
Lixia Dai ◽  
Jian He ◽  
Xiaolou Miao ◽  
Xiao Guo ◽  
Xiaofei Shang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Underlying Mechanism ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Active Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Tof Ms

Backgroud:Rhododendron przewalskii Maxim. is an evergreen shrub that is used as a traditional medicine in China. However, the modern pharmacology and the chemical components of this plant has not been studied. In this paper, we aimed to investigate the antifungal, anti-inflammatory and antioxidant activities and underlying mechanism of its aqueous and ethanol extracts, and analyze their chemical composition and active compounds of R. przewalskii.Methods: The antifungal activity was determined in vitro, and anti-inflammatory and antioxidant activities and underlying mechanism of its aqueous and ethanol extracts were evaluated in vitro and in RAW 264.7 cells. The chemical composition were analyzed using UPLC-ESI-Q-TOF/MS, and the contents of six compounds were determined via HPLC.Results: Both extracts of R. przewalskii showed promising anti-inflammatory activity in vitro; decreased the production of four inflammatory cytokines, namely, nitric oxide, IL-1β, IL-6 and TNF-ɑ, in RAW 264.7 cells induced by lipopolysaccharide; and exhibited weak cytotoxicity. The extracts significantly scavenged DPPH radicals, superoxide radicals and hydroxyl radicals to exert antioxidant effects in vitro. The two extracts also exhibited cellular antioxidant activity by increasing superoxide dismutase and CAT activities and decreasing malondialdehyde content in RAW 264.7 cells induced by LPS. However, the antifungal activity of the two extracts was weak. Nine flavonoids were identified by UPLC-ESI-Q-TOF/MS. Of these, six compounds were analyzed quantitatively, including avicularin, quercetin, azaleatin, astragalin and kaempferol, and five compounds (myricetin 3-O-galactoside, paeoniflorin, astragalin, azaleatin and kaempferol) were found in this species for the first time. These compounds demonstrated antioxidant activities that were similar to those of the R. przewalskii extracts and were thought to be the active compounds in the extracts.Conclusion:R. przewalskii extracts presented promising anti-inflammatory and antioxidant activities. The extracts contained amounts of valuable flavonoids (8.98 mg/g fresh material) that were likely the active compounds in the extract contributing to the potential antioxidant activity. These results highlight the potential of R. przewalskii as a source of natural antioxidant and anti-inflammatory agents for the pharmaceutical industry.

Cinnamaldehyde and eugenol protect against LPS‐stimulated oxidative stress and inflammation in Raw 264.7 cells

2021 ◽  
Author(s):  
Emine Gulceri Gulec Peker ◽  
Kaan Kaltalioglu
Keyword(s):  
Oxidative Stress ◽  
Raw 264.7 Cells ◽  
Raw 264.7

Comparison of the Biological Activity Between Ultrafine and Fine Titanium Dioxide Particles in RAW 264.7 Cells Associated with Oxidative Stress

2008 ◽  
Vol 71 (8) ◽  
pp. 478-485 ◽  
Author(s):  
Jihee Lee Kang ◽  
Changsuk Moon ◽  
Hui Su Lee ◽  
Hae Won Lee ◽  
Eun-Mi Park ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Titanium Dioxide ◽  
Biological Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7

scholarly journals Diarylheptanoids of Curcuma comosa with Inhibitory Effects on Nitric Oxide Production in Macrophage RAW 264.7 Cells

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Nilubon Sornkaew ◽  
Yuan Lin ◽  
Fei Wang ◽  
Guolin Zhang ◽  
Ratchanaporn Chokchaisiri ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
Raw 264.7 Cells ◽  
Racemic Mixture ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Inhibitory Effects ◽  
Curcuma Comosa ◽  
Oxide Production ◽  
Potent Inhibitory Activity

Eight new diarylheptanoids, a 1.2:1 mixture of (3S)- and (3 R)-1-(4-hydroxyphenyl)-7-phenyl-(4 E,6 E)-4,6-heptadien-3-ol (1a and 1b), a racemic mixture of (3S)- and (3 R)-1-(4-hydroxyphenyl)-3-methoxy-7-phenyl-(4 E,6 E)-4,6-heptadiene (2a and 2b), a ca. 1:1 mixture of (3S)- and (3 R)-1-(4-hydroxy-3-methoxyphenyl)-3-methoxy-7-phenyl)-(4 E,6 E)-4,6-heptadiene (3a and 3b), 3-acetoxy-1-(3,4-dihydroxyphenyl)-7-phenylheptan-5-ol (4), (3 R)-1-(4,5-dihydroxyphenyl)-7-phenyl-(6 E)-6-hepten-3,2′-epoxide (5), and thirteen known diarylheptanoids, 6-12, a 3:1 mixture of 13a and 13b, and 14-17, were isolated from the rhizomes of Curcuma comosa from Sakon Nakhon, northeastern part of Thailand. The isolated compounds were evaluated for their antiinflammatory activities on the inhibition of lipopolysaccharide-induced nitric oxide production in macrophage RAW 264.7 cells and the diarylheptanoids 1a and 1b mixture and 14 exhibited potent inhibitory activity.

scholarly journals Novel and Stable Dual-Color IL-6 and IL-10 Reporters Derived from RAW 264.7 for Anti-Inflammation Screening of Natural Products

2019 ◽  
Vol 20 (18) ◽  
pp. 4620
Author(s):  
Papawee Saiki ◽  
Yasuhiro Kawano ◽  
Yoshihiro Nakajima ◽  
Leo J. L. D. Van Griensven ◽  
Koyomi Miyazaki
Keyword(s):  
Natural Products ◽  
Real Time ◽  
Inflammatory Disease ◽  
Chronic Inflammatory Disease ◽  
New Drugs ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Screening Assay ◽  
Dual Color ◽  
Ethanol Extracts

Our previous study suggested that the interleukin (IL)-6 and IL-10 could serve as good biomarkers for chronic inflammatory disease. We previously established an IL-6 and IL-10 reporters assay that could examine reporter activity along with the reference gene in LPS-induced RAW 264.7 cells. In this study, we described new and stable RAW 264.7 derived dual-color IL-6/gapdh and IL-10/gapdh reporters. This assay allowed us to easily determine relative IL-6 and IL-10 levels with 96-well plate within one step. We evaluated the relative IL-6 and IL-10 levels in the LPS-induced stable cells testing 52 natural products by real-time bioluminescence monitoring and time-point determination using a microplate luminometer. The relative IL-6 and IL-6/IL-10 values decreased by the crude ethanol extracts from nutmeg and by 1′S-1′-acetoxychavicol from greater galangal using real-time bioluminescence monitoring. At the same time, the relative IL-10 was induced. The relative IL-6 and IL-6/IL-10 decreased by crude ethanol extracts from nutmeg and 1′S-1′-acetoxychavicol acetate at 6 h. Only crude ethanol extract from nutmeg induced IL-10 at 6 h. We suggested that the use of these stable cells by real-time monitoring could serve as a screening assay for anti-inflammatory activity and may be used to discover new drugs against chronic inflammatory disease.

scholarly journals Correction: Inhibitory Effect of Ginsenosides Rh1 and Rg2 on Oxidative Stress in LPS-Stimulated RAW 264.7 Cells

2019 ◽  
Vol 49 (2) ◽  
pp. 93
Author(s):  
Yujin Jin ◽  
Naehwan Baek ◽  
Soyoung Back ◽  
Chang-Seon Myung ◽  
Kyung-Sun Heo
Keyword(s):  
Oxidative Stress ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7
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