scholarly journals Optimization for ultrasonic-microwave synergetic extraction of total iridoid glycosides and screening of analgesic and anti-inflammatory active fractions from patrinia scabra Bunge (Valerianaceae)

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Quhuan Ma ◽  
Yanmei Lu ◽  
Yi Deng ◽  
Xiaodong Hu ◽  
Wanyu Li ◽  
...  

Abstract Background Patrinia scabra Bunge is a well-known herbal medicine for its favorable treatment on inflammatory diseases owing to its effective ingredients, in which iridoid glycoside plays an extremely significant role. This article aimed to improve the content of total iridoid glycosides in crude extract through a series optimization of extraction procedure. Moreover, considering that both pain and inflammation are two correlated responses triggered in response to injury, irritants or pathogen, the article investigated the anti-inflammatory and analgesic activities of P. scabra to screen out the active fraction. Method P. scabra was extracted by ultrasonic-microwave synergistic extraction (UMSE) to obtain total iridoid glycosides (PSI), during which a series of conditions were investigated based on single-factor experiments. The extraction process was further optimized by a reliable statistical method of response surface methodology (RSM). The elution fractions of P. scabra extract were prepared by macroporous resin column chromatography. Through the various animal experiment including acetic acid-induced writhing test, formalin induced licking and flinching, carrageenan-induced mice paw oedema test and xylene-induced ear edema in mice, the active fractions with favorable analgesic and anti-inflammatory effect were reasonably screen out. Results The content of PSI could reach up to 81.42 ± 0.31 mg/g under the optimum conditions as follows: ethanol concentration of 52%, material-to-liquid ratio of 1:18 g/mL, microwave power at 610 W and extraction time of 45 min. After gradient elution by the macroporous resin, the content of PSI increased significantly. Compared with other concentrations of elution liquid, the content of PSI in 30 and 50% ethanol eluate was increased to reach 497.65 and 506.90 mg/g, respectively. Owing to the pharmacology experiment, it was reasonably revealed that 30 and 50% ethanol elution fractions of P. scabra could relieve pain centrally and peripherally, exhibiting good analgesic and anti-inflammatory activities. Conclusion Patrinia scabra possessed rich iridoids and exhibited significant analgesic and anti-inflammatory activities.

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
K. Lalrinzuali ◽  
M. Vabeiryureilai ◽  
Ganesh Chandra Jagetia

Inflammation is all a pervasive phenomenon, which is elicited by the body in response to obnoxious stimuli as a protective measure. However, sustained inflammation leads to several diseases including cancer. Therefore it is necessary to neutralize inflammation. Sonapatha (Oroxylum indicum), a medicinal plant, is traditionally used as a medicine in Ayurveda and other folk systems of medicine. It is commonly used to treat inflammatory diseases including rheumatoid arthritis and asthma. Despite this fact its anti-inflammatory and analgesic effects are not evaluated scientifically. Therefore, the anti-inflammatory and analgesic activities of Sonapatha (Oroxylum indicum) were studied in Swiss albino mice by different methods. The hot plate, acetic acid, and tail immersion tests were used to evaluate the analgesic activity whereas xylene-induced ear edema and formalin induced paw edema tests were used to study the anti-inflammatory activity of Sonapatha. The administration of mice with 250 and 300 mg/kg b.wt. ofO. indicumreduced pain and inflammation indicating that Sonapatha possesses analgesic and anti-inflammatory activities. The maximum analgesic and anti-inflammatory activities were observed in mice receiving 300 mg/kg b.wt. ofO. indicumethanol extract.Our study indicates thatO. indicumpossesses both anti-inflammatory and analgesic activities and it may be useful as an anti-inflammatory agent in the inflammation related disorders.


Medicina ◽  
2018 ◽  
Vol 54 (4) ◽  
pp. 51 ◽  
Author(s):  
Marzieh Pashmforosh ◽  
Hossein Rajabi Vardanjani ◽  
Hassan Rajabi Vardanjani ◽  
Mahdi Pashmforosh ◽  
Mohammad Khodayar

Background and objectives:Citrullus colocynthis (CC), known as bitter apple, is used to treat diabetes in Iranian traditional medicine. The aim of this study is to evaluate the anti-inflammatory and analgesic activities of CC cream in rats. Materials and Methods: The carrageenan-induced edema in a rat hind paw was carried out to evaluate the topical anti-inflammatory effect of the CC fruit extract cream (2–8%) and the tissue levels of IL-6 and TNF-α were estimated by using a commercial ELISA kit. The topical antinociceptive activity of CC cream (2–8%) was evaluated in the rat formalin test. To determine the role of opioid receptors in the local antinociceptive effect of the CC cream, naloxone (20 μg/paw, i.pl.), a non-selective opioid antagonist, was used. Results: The results showed that the CC cream (2–8%) dose-dependently reduced the carrageenan-induced paw edema and reversed the changes in the level of TNF-α and IL-6 due to carrageenan-induced edema (p < 0.01). The anti-inflammatory effect of CC cream 8% was comparable to that of hydrocortisone ointment 1%. Furthermore, the application of CC cream (2–8%) dose-dependently inhibited both first and second phases of the formalin test (p < 0.05). The antinociceptive effect of the CC cream (8%) was comparable to that of methyl salicylate cream 30%. Moreover, the administration of naloxone significantly reversed the topical antinociceptive effect of the CC cream (p < 0.05). Conclusions: For the first time, this study indicated that the topical application of CC cream possesses significant anti-inflammatory and antinociceptive activities in animal models, which were probably mediated by opioid receptors and the suppression of pro-inflammatory cytokines (TNF-α and IL-6). Thus, the CC cream can be used to treat inflammatory pain and inflammatory diseases.


INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (09) ◽  
pp. 5-12
Author(s):  
S. S Mahajan ◽  
◽  
A. D. Gaitonde

Ten compounds from the series of 2-amino-5-(substituted)phenyl-1,3,4-thiadiazoles were synthesized and evaluated for anti-inflammatory and analgesic activities. Their structures were characterized by infrared spectroscopy and confirmed by 1H NMR spectroscopy. Anti-inflammatory activity was carried out by carrageenan-induced rat paw oedema method using indomethacin as standard. Analgesic activity was performed by acetic-acid induced writhing method in mice using aspirin as standard. The QSAR studies were performed for the analgesic activity using the software ‘Strike’ from Schrodinger. Docking studies were carried out for COX-2 inhibition using the software ‘Glide’ from Schrodinger. The compounds were obtained in good yields and possessed good anti-inflammatory and analgesic activities. Various physicochemical parameters, contributing towards analgesic activity were identified from the QSAR studies. The Glide Scores were more or less correlated with the anti-inflammatory and analgesic activities of compounds.


2007 ◽  
Vol 62 (11-12) ◽  
pp. 813-820 ◽  
Author(s):  
Esra Küpeli Akkol ◽  
I. Irem Tatli ◽  
Zeliha S. Akdemir

The anti-inflammatory and antinociceptive properties of four major compounds from the flowers of Verbascum pterocalycinum var. mutense were investigated. Saponin glycosides called ilwensisaponin A and C and iridoid glycosides known as ajugol and picroside IV were isolated from the methanolic extract. A dose-related anti-inflammatory and antinociceptive response were obtained in this study at doses of 100 and 200 mg/kg. The results of the evaluation of the anti-inflammatory activity induced by carrageenan and PGE1 showed that this species possesses active constituents that could diminish the cyclooxygenase activitiy. No effects were observed in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model. Our results support the anti-inflammatory and analgesic effects of Verbascum pterocalycinum var. mutense. Ilwensisaponins A and C could explain in part the anti-inflammatory and analgesic activities of this species. Although antinociceptive and anti-inflammatory activities of ajugol and picroside IV were found insignificant in the statistical analysis, ilwensisaponin A and C showed notable activity without inducing any apparent acute toxicity as well as gastric damage.


Author(s):  
Adjaratou C. Coulibaly ◽  
W. L. M. Esther B. Kabre ◽  
Tata K. Traore ◽  
Constantin Achatde ◽  
Gilchrist L. Boly ◽  
...  

Aims: Acacia macrostachya Reichenb. ex DC. (Fabaceae) is used in traditional medicine for the treatment of many pathologies including diarrhea, malaria with convulsion and fevers, snake bites, vomiting, nausea, dysenteric syndrome and choleriformis, inflammatory diseases and old wounds. The purpose of this study was to carry out the phytochemical screening, to assess the general acute toxicity, the anti-inflammatory and analgesic activities of the dichloromethane extract of A. macrostachya root barks in order to develop new lower-toxic anti-inflammatory drugs. Place and Duration of Study: The work was carried out in the Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Institute for Research in Health Sciences (IRSS) and LABIOCA from Université Joseph KI-ZERBO in Ouagadougou between March and June 2020. Methodology: Phytochemical screening was carried out through thin layer chromatography with specific reagents. Acute toxicity assay was carried out according to the "dose adjustment" method from the OECD guideline 423 2001. Analgesic effect was evaluated on the number of abdominal contortions induced by the intraperitoneal injection of acetic acid while the anti-inflammatory activity using the Carrageenan anti-edematous test was determined according to Winter. Results: Phytochemical profile demonstrated the presence of alkaloids, coumarins, polyphenols, tannins, flavonoids, saponosides, triterpenes and sterols. At the dose of 200 mg/kg (bw), the extract inhibited acetic acid-induced pain by 67.08% and carragenaan-induced edema by 74.03%. A. macrostachya dichloromethane extract is slightly toxic with LD50 higher than 5000 mg/kg (bw). Conclusion:The results of this study demonstrated the interest of A. macrostachya in the treatment of inflammatory pathologies and constitute a scientific basis of its traditional uses.


Author(s):  
Asie Shojaii ◽  
Manijeh Motevalian ◽  
Nazanin Rahnama

AbstractThe importance of inflammatory diseases and side effects of conventional drugs necessitate the finding of new anti-inflammatory agents from natural sources. In this study, for the first time, the anti-inflammatory and analgesic effects of the aqueous extract ofThirty-five male Wistar rats were divided into five groups and pretreated with different doses ofThe extract ofThe aqueous extract of


2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Nara Lins Meira Quintão ◽  
Lilian W. Rocha ◽  
Gislaine Franciele Silva ◽  
Simone Reichert ◽  
Vanessa D. Claudino ◽  
...  

The aim of the study was to analyze the constituents of the dichloromethane fraction obtained fromA. moluccanaand also to evaluate the anti-inflammatory and antinociceptive properties ofα,β-amyrenone isolated fromA. moluccanain mice. The dichloromethane fraction was evaluated by gas chromatography and submitted to purification. The mixture ofα,β-amyrenone was isolated and then evaluated using the carrageenan-induced paw-oedema or pleurisy and CFA-induced arthritis models in mice. Five triterpenes,α,β-amyrenone, glutinol, andα,β-amyrin were isolated from dichloromethane fraction ofA. moluccanaleaf extract. The mixture ofα,β-amyrenone, dosed orally, was able to reduce mechanical hypersensitivity and paw-oedema induced by carrageenan, interfering with neutrophil migration. Similar results were observed in the carrageenan-induced pleurisy model. Repeated administration of the compounds was also effective in reducing the mechanical sensitization and oedema developed in the arthritis model induced by CFA. In conclusion, the results demonstrate thatα,β-amyrenone interferes in both acute and chronic inflammatory processes. We can infer that these effects involve, at least in part, a reduction in the neutrophil migration. Therefore, it seems reasonable to suggest thatα,β-amyrenone could represent a new therapeutic tool for the management of painful and inflammatory diseases, especially those presenting a chronic profile.


2018 ◽  
Vol 1 (2) ◽  
pp. 85-94
Author(s):  
M.M. Onakpa

Inflammation is a pervasive phenomenon elicited by the body in response to obnoxious stimuli as a protective measure. However, a sustained inflammation lead to several diseases including cancer therefore the necessity to neutralize inflammation is paramount. Mitragyna inermis is a medicinal plant traditionally used as a medicine in Ayurveda and other folk systems of medicine. It is commonly used to treat inflammatory diseases including rheumatoid arthritis and asthma. Despite this fact its anti-inflammatory and analgesic effects have not been evaluated scientifically. Therefore, the anti-inflammatory and analgesic activities of M. inermis were studied in Wistar rats by different methods. The hot plate, acetic acid, and tail immersion tests were used to evaluate the analgesic activity whereas paw edema model for acute inflammation using egg albumin were used to study the anti-inflammatory activity. The administration of 250 and 300 mg/kg to rats reduced pain and inflammation indicating that M. inermis possesses analgesic and anti-inflammatory activities. The maximum analgesic and anti-inflammatory activities were observed in rats receiving 300 mg/kg of M. inermis extract. This study indicates that methanolic extract of M. inermis possess both anti-inflammatory and analgesic activiti


2017 ◽  
Vol 8 (3) ◽  
pp. 489 ◽  
Author(s):  
Andini Bakti Putri ◽  
Anita Anita

<p>Inflammation is a complex reaction of the body's immune system in the vascular tissue that causes the accumulation and activation of leukocytes and plasma proteins that occur during infection, poisoning or cell damage. Inflammation can be treated by using anti-inflammatory drugs such as NSAIDs (Non-Steroidal Anti-Inflammatory Drugs) that serve as pain reliever, febrifuge, and anti-inflammatory. One of the most common chronic inflammatory diseases is osteoarthritis. Osteoarthritis is the most common form of arthritis in society,which has a major impact on public health problems. Currently there are many therapeutic modalities of both pharmacology and non-pharmacology for the management of osteoarthritis, therefore it is necessary to recommend effective treatment of osteoarthritis in Indonesia based on the latest scientific evidence. The use of NSAIDs that do not correspond to doses can cause side effects such as bleeding, gastric ulcers, worsening asthma symptoms and acute kidney failure. This is the health problem in Indonesia, especially for ordinary people who use NSAIDs with excessive doses, because they want to quickly relieve pain. Therefore, it is necessary to look for a safe anti-inflammatory drug and can reduce the pain caused to arthritis. <em>Ananascomosus</em> (pineapple) has long been used for various medical purposes. There are four different proteases in the pineapple, the two major enzymes in pineapple defined as bromelain’s stems and bromelain’s fruit. In the latest study there are various therapeutic benefits that have been proposed for bromelin, such as anti-inflammatory, reducing swelling, reducing pain, facilitating the digestive system, accelerating wound healing and acting as an anticoagulant.<strong></strong></p>


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