Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

The objective of this experiment was to determine the effects on mammary carcinogenesis of similar limitations in energy availability either by energy expenditure due to moderate-intensity running (physical activity, PA) or by regulating dietary energy (RE) intake relative to a sedentary control (SC) group that ate ad libitum. A total of 90 female Sprague-Dawley rats were injected with 1-methyl-1-nitrosourea (50 mg/kg) and 7 days thereafter were randomized to either SC, a PA group given free access to a motorized running wheel, or a RE group whose food intake limited growth to the rate observed in PA. Compared with SC, mammary carcinogenesis was inhibited by RE or PA. Cancer incidence, 92.6%, 77.8%, and 66.7% ( P = 0.06), and cancer multiplicity, 3.44, 2.11, and 1.62 cancers/rat ( P = 0.006), in SC, RE, and PA, respectively, were reduced to a similar extent by RE and PA. Histological and Western blot analyses of mammary carcinomas provided evidence that RE and PA induced apoptosis via the mitochondrial pathway, that cell cycle progression was suppressed at the G1/S transition, and that intratumoral blood vessel density was reduced, although it remains to be determined whether PA and RE exert these effects via the same mechanisms.

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The effects of MOTILIPERM on cisplatin induced testicular toxicity in Sprague–Dawley rats

Cancer Cell International ◽

10.1186/s12935-015-0274-1 ◽

2015 ◽

Vol 15 (1) ◽

Cited By ~ 13

Author(s):

Kiran Kumar Soni ◽

Li Tao Zhang ◽

Jae Hyung You ◽

Sung Won Lee ◽

Chul Young Kim ◽

...

Keyword(s):

Testicular Toxicity ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Anti-infertility significance of aqueous extract of I pomoea batatas (L.) Lam. against exposure of bisphenol A (BPA) promoted testicular toxicity in male Sprague Dawley rats

Asian Pacific Journal of Reproduction ◽

10.1016/s2305-0500(13)60160-x ◽

2013 ◽

Vol 2 (4) ◽

pp. 263-271

Author(s):

Rajendran Revathy ◽

Kulanthaivel Langeswaran ◽

Subbaraj Gowtham kumar ◽

Shanmugam Vijayaprakash ◽

Peranandam Tamilselvan ◽

...

Keyword(s):

Bisphenol A ◽

Aqueous Extract ◽

Testicular Toxicity ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Senecio biafrae defeated Tetracycline-Induced Testicular Toxicity in Adult Male Sprague Dawley Rats

JBRA Assisted Reproduction ◽

10.5935/1518-0557.20180054 ◽

2018 ◽

Author(s):

Sunday Adelakun ◽

Olusegun Omotoso ◽

Julius Aniah ◽

Oyebowale Oyewo

Keyword(s):

Adult Male ◽

Testicular Toxicity ◽

Sprague Dawley ◽

Sprague Dawley Rats

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The ameliorative effect of monotropein, astragalin, and spiraeoside on oxidative stress, endoplasmic reticulum stress, and mitochondrial signaling pathway in varicocelized rats

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2736-9 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 9

Author(s):

Keshab Kumar Karna ◽

Bo Ram Choi ◽

Jae Hyung You ◽

Yu Seob Shin ◽

Wan Shou Cui ◽

...

Keyword(s):

Oxidative Stress ◽

Endoplasmic Reticulum ◽

Er Stress ◽

Regulatory Protein ◽

Serum Testosterone ◽

Mitochondrial Pathway ◽

Sprague Dawley ◽

Testicular Germ Cell ◽

Signaling Mechanism ◽

Tnf Α

Abstract Background Monotropein, astragalin, and spiraeoside (MAS) are active compounds extracted from medicinal herbs; monotropein from Morinda officinalis How (Rubiaceae), astragalin (kaempferol 3-O-glucoside) from Cuscuta chinensis Lamark (Convolvulaceae) and spiraeoside from the outer scales of Allium cepa L. (Liliceae) in a ratio of 6.69:0.41:3.61. Monotropein, astragalin, and spiraeoside are well-known antioxidants, anti-inflammatory, and antinociceptive agents. The current investigation aims to study the molecular mechanism of varicocele-induced male infertility and the underlying pharmacological mechanisms of MAS. Methods Four groups were included: control (CTR), MAS 200 group (MAS 200 mg/kg), varicocele group (VC), and VC + MAS 200 group (MAS 200 mg/kg). Sprague-Dawley (SD) rats were treated with 200 mg/kg MAS or vehicle once daily for 28 days. The possible signaling mechanism and effects of MAS were measured via histological staining, immunohistochemistry, western blot, and biochemical assays. Results Parameters such as sperm motility and count, Johnsen’s scores, spermatogenic cell density, serum testosterone, testicular superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and expression of the steroidogenic acute regulatory protein (StAR) improved significantly in the VC + MAS 200 group compared with the VC group. MAS treatment of varicocele-induced group significantly decreased the levels of serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as testicular interleukin-6 (IL6), tumor necrosis factor-α (TNF-α), ROS/RNS, and malondialdehyde (MDA). It also decreased the apoptotic index and reduced the expression of endoplasmic reticulum (ER) protein levels (Grp78, p-IRE1α, and p-JNK) and apoptotic markers such as cleaved caspase-3 and Bax/Bcl2 ratio. Conclusion This study suggests that the crosstalk between oxidative stress, ER stress, and mitochondrial pathway mediates varicocele-induced testicular germ cell apoptosis. MAS promotes spermatogenesis in varicocele-induced SD rat, probably by decreasing cytokines (IL-6, TNF-α) levels, regulating abnormal sex hormones, and decreasing oxidative stress, ER stress, and apoptosis.

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Delayed effects of autophagy on T-2 toxin-induced apoptosis in mouse primary Leydig cells

Toxicology and Industrial Health ◽

10.1177/0748233719831122 ◽

2019 ◽

Vol 35 (3) ◽

pp. 256-263 ◽

Cited By ~ 4

Author(s):

Jian Ying Yang ◽

Yong Fa Zhang ◽

Xiang Ping Meng ◽

Xiang Feng Kong

Keyword(s):

Cross Talk ◽

Leydig Cells ◽

Caspase 3 ◽

Treated Group ◽

Testicular Toxicity ◽

Reproductive Disorders ◽

Delayed Effects ◽

Food Commodities ◽

Induced Apoptosis ◽

High Level

T-2 toxin is a type-A trichothecene produced by Fusarium found in several food commodities worldwide. T-2 toxin causes reproductive disorders, genotoxicity, and testicular toxicity in animals. Our previous research has reported that T-2 toxin can induce apoptosis via the Bax-dependent caspase-3 activation in mouse primary Leydig cells. However, little is known about the functions of autophagy and the cross talk between autophagy and apoptosis after exposure to T-2 toxin in Leydig cells. This study investigated these problems in mouse primary Leydig cells. Results showed that T-2 toxin treatment upregulated LC3-II and Beclin-1 expression, suggesting that T-2 toxin induced a high level of autophagy. Pretreatment with chloroquine (an autophagy inhibitor) and rapamycin (an autophagy inducer) increased and decreased the rate of apoptosis, respectively, in contrast to T-2 toxin-treated group. Autophagy delayed apoptosis in the T-2 toxin-treated Leydig cells. Therefore, autophagy may prevent cells from undergoing apoptosis by reducing T-2 toxin-induced cytotoxicity.

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Propofol Improved Glucose Tolerance Associated with Increased FGF-21 and GLP-1 Production in Male Sprague-Dawley Rats

Molecules ◽

10.3390/molecules25143229 ◽

2020 ◽

Vol 25 (14) ◽

pp. 3229

Author(s):

Chih-Cheng Wu ◽

Chih-Jen Hung ◽

Ya-Yu Wang ◽

Shih-Yi Lin ◽

Wen-Ying Chen ◽

...

Keyword(s):

Glucose Metabolism ◽

Er Stress ◽

Chloral Hydrate ◽

Fibroblast Growth Factor 21 ◽

Sprague Dawley ◽

Mortality And Morbidity ◽

Glucose Levels ◽

Sprague Dawley Rats ◽

Old Rats ◽

Glucagon Like Peptide 1

Anesthetics, particularly volatile anesthetics, have been shown to impair glucose metabolism and cause hyperglycemia, closely linking them with mortality and morbidity as related to surgery. Beyond being an anesthetic used for general anesthesia and sedation, intravenous hypnotic propofol displays an effect on glucose metabolism. To extend the scope of propofol studies, its effects on glucose metabolism were evaluated in male Sprague-Dawley rats of various ages. Unlike chloral hydrate and isoflurane, propofol had little effect on basal glucose levels in rats at 2 months of age, although it did reduce chloral hydrate- and isoflurane-induced hyperglycemia. Propofol reduced postload glucose levels after either intraperitoneal or oral administration of glucose in both 7- and 12-month-old rats, but not those at 2 months of age. These improved effects regarding propofol on glucose metabolism were accompanied by an increase in insulin, fibroblast growth factor-21 (FGF-21), and glucagon-like peptide-1 (GLP-1) secretion. Additionally, an increase in hepatic FGF-21 expression, GLP-1 signaling, and FGF-21 signaling, along with a decrease in endoplasmic reticulum (ER) stress, were noted in propofol-treated rats at 7 months of age. Current findings imply that propofol may turn into insulin-sensitizing molecules during situations of existing insulin resistance, which involve FGF-21, GLP-1, and ER stress.

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