scholarly journals Alterations in complex lipids in tumor tissue of patients with colorectal cancer

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Alicja Pakiet ◽  
Kinga Sikora ◽  
Jarek Kobiela ◽  
Olga Rostkowska ◽  
Adriana Mika ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Lipid Metabolism ◽  
Cell Membrane ◽  
Membrane Properties ◽  
Cancer Tissue ◽  
Bioactive Lipids ◽  
Colon Cancer Tissue ◽  
Complex Lipids ◽  
Lipid Compounds

Abstract Background Accumulating evidence indicates alterations in lipid metabolism and lipid composition in neoplastic tissue. Earlier nuclear magnetic resonance studies showed that the contents of major lipid groups, such as triacylglycerols, phospholipids and cholesterol, are changed in colon cancer tissue. Methods In this study, a more detailed analysis of lipids in cancer and tumor adjacent tissues from colorectal cancer patients, using liquid chromatography–mass spectrometry, allowed for comparison of 199 different lipids between cancer tissue and tumor adjacent tissue using principal component analysis. Results Significant differences were found in 67 lipid compounds between the two types of tissue; many of these lipid compounds are bioactive lipids such as ceramides, lysophospholipids or sterols and can influence the development of cancer. Additionally, increased levels of phospholipids and sphingolipids were present, which are major components of the cell membrane, and increases in these lipids can lead to changes in cell membrane properties. Conclusions This study showed that many complex lipids are significantly increased or decreased in colon cancer tissue, reflecting significant alterations in lipid metabolism. This knowledge can be used for the selection of potential molecular targets of novel anticancer strategies based on the modulation of lipid metabolism and the composition of the cell membrane in colorectal cancer cells.

scholarly journals Research for Expression and Prognostic Value of GABRD in Colon Cancer and Coexpressed Gene Network Construction Based on Data Mining

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Tao Liu ◽  
Yuejun Fang
Keyword(s):  
Data Mining ◽  
Colon Cancer ◽  
Normal Tissue ◽  
Predictive Ability ◽  
Gene Interaction ◽  
Notch Signaling Pathway ◽  
Cancer Tissue ◽  
Gamma Aminobutyric Acid ◽  
Colon Cancer Tissue ◽  
Go Annotation

Colon cancer is one of the top five cancers with the highest incidence rate in the world. In order to better understand the pathogenesis and progression of colon cancer, it is still necessary to investigate the abnormally expressed genes in cancer tissue. In this study, the Oncomine database was used for expression analysis, and it was found that the expression level of gamma-aminobutyric acid type A receptor subunit delta (GABRD) gene was upregulated in colon cancer tissue compared with that in normal tissue. UALCAN was used to analyze the expression of GABRD in different groups of age, gender, cancer stage, N stage, and histological subtype. Then, it was also found that the expression of GABRD in each subgroup of colon cancer tissue was all high compared with that in normal tissue. LinkedOmics was used to screen out the differentially expressed genes related to GABRD expression in colon cancer. GO annotation and KEGG pathway enrichment analyses found that the correlated genes may be related to breast cancer, human papillomavirus infection, Notch signaling pathway, and other pathways. Thereafter, GSEA was performed to obtain GABRD-related kinases, miRNAs, and transcription factors, and gene interaction networks were constructed. It was found that GABRD may be involved in cell cycle regulation. Finally, websites like GEPIA were used to detect the predictive ability of GABRD on the prognosis of patients with colon cancer. Kaplan-Meier analysis suggested that the upregulation of GABRD expression was related to the poor prognosis of patients with colon cancer. Overall, in this study, the potential role and prognostic ability of GABRD in colon cancer were explored through data mining, which can be a clue for further research on GABRD.

Abstract 4944: Detection of functional P-selectin ligands expressed on colon cancer tissue using a novel flow-based assay

2016 ◽  
Author(s):  
Eric W. Martin ◽  
Ramiro Malgor ◽  
Vicente A. Resto ◽  
Douglas J. Goetz ◽  
Monica M. Burdick
Keyword(s):  
Colon Cancer ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Selectin Ligands

scholarly journals Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue

2007 ◽  
Vol 1 (2) ◽  
pp. 205-215 ◽  
Author(s):  
P.A. Usher ◽  
A.M. Sieuwerts ◽  
A. Bartels ◽  
U. Lademann ◽  
H.J. Nielsen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Alternatively Spliced

Free-radical processes in multifocal colon cancer tissue.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e14636-e14636
Author(s):  
Michail L. Maleyko ◽  
Elena Frantsiyants ◽  
Yuri Gevorkyan ◽  
Ekaterina Komarova ◽  
Andrey Dashkov ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Free Radical ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Free Radical Processes ◽  
Radical Processes

Adherent E. coli from colon cancer tissue can induce IL-8 release and COX2 expression

2003 ◽  
Vol 124 (4) ◽  
pp. A484
Author(s):  
Helen Martin ◽  
Barry Campbell ◽  
Manu Nayar ◽  
Helen Williams ◽  
Charles A. Hart ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
E Coli ◽  
Cox2 Expression

scholarly journals The expression and Clinical Significance of miRNA-135a and Bach1 in colorectal cancer

2021 ◽  
Author(s):  
Yang zhi Jiang ◽  
Qing Guo Tao ◽  
fei yan Zhu
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Clinical Information ◽  
Control Group ◽  
Tumor Control ◽  
Cancer Tissue ◽  
Expression Levels ◽  
Real Time Quantitative Pcr ◽  
Normal Tissues ◽  
Cancer Tissues

BACKGROUND AIM To explore the correlation between the expression of miRNA-135a and Bach1 in colorectal cancer tissue and the patient's clinical information.  Methods   60 patients with colorectal carcinoma were treated as a control group. Real-time quantitative PCR assays and immunohistochemistry method were performed to detect the expression of miRNA-135a and Bach1 in 60 colorectal carcinomas and adjacent normal tissues, and the clinical and pathological classifications had also been investigated. The SPSS 19.00 software was used. All data represented mean±SD of three independent experiments. P<0.05 was considered statistically significant. Results  miRNA-135a expression levels increased significantly in the colon cancer tissues compared with the non-tumor control tissues(P<0.01). miRNA-135a expression levels were higher in stage III/IV than in stage I/II colon cancer patients. The expression level of Bach1 in colorectal cancer was significantly lower(P<0.01). Bach1 and miRNA-135a were negatively correlated.  Conclusions:  The levels of miRNA-135a and Bach1 were opposite, the over-expression of miRNA-135a might downregulated the expression of Bach1, which might be involved in the pathogenesis of colorectal cancer.

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