Cervical human papillomavirus infection in Serbia: Risk factors, prevalence and genotype distribution in women with normal cervical cytology

Sexual behavioral and other risk factors and the prevalence of genital human papillomavirus (HPV) infection are very important for the monitoring of HPV infection and cervical cancer prevention strategies. The purpose of this study was to determine the risk factors, prevalence of cervical HPV infection and genotype distribution among asymptomatic young women with normal cytology in Serbia. A total of 204 consenting young women were enrolled in this study and interviewed about risk factors. The presence of HPV DNA was assessed using the PCR method. HPV genotypes were identified by direct sequencing. Cervical HPV infection was detected in 19.1% of women. Out of nine identified HPV genotypes, types 16 and 52 were the most frequent. A significant association was found only between the number of sexual partners and HPV positivity (p<0.05). The obtained results showed the high prevalence of high-risk HPV types among young women in Serbia.

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Prevalence and distribution of human papillomavirus genotypes among women in northern Henan Province of China

10.21203/rs.3.rs-889505/v1 ◽

2021 ◽

Author(s):

Xiangpeng Wang ◽

Yuan Song ◽

Xiaofei Wei ◽

Guanyu Wang ◽

Ruili Sun ◽

...

Keyword(s):

Human Papillomavirus ◽

Age Groups ◽

Hpv Infection ◽

Cervical Cancer Prevention ◽

Henan Province ◽

Infection Prevalence ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Prevalence ◽

Specific Hybridization

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers including cancer of vulva, vagina, penis, anus, or oropharynx. However, data concerning the prevalence and genotype distribution of HPV among women are limited in northern Henan Province of China. This study aimed to make an investigation on the current prevalence and genotype distribution of HPV among women and provided comprehensive data to guide HPV-based cervical cancer prevention in northern Henan Province. Methods A total of 15616 women aged 16 to 81 years who attended the department of gynecology of Xinxiang central hospital between January 2018 and December 2019 were enrolled in this study. HPV DNA was detected by PCR method followed by HPV type-specific hybridization. The overall prevalence, age-specific prevalence and genotype distribution of HPV were investigated. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double and multiple HPV infections accounted for 13.7%, 4.3% and 1.8% of the total cases, respectively. HPV prevalence was 41.8% among women aged 16 to 19 years, 21.1% among women aged 20 to 29 years, 18.1% among women aged 30 to 39 years, 17.7% among women aged 40 to 49 years, 20.8% among women aged 50 to 59 years, 22.9% among women more than 60 years. HPV infection rates differed significantly across different age groups. Most infections were caused by high-risk HPV (HR-HPV) and single genotype HPV infection was the most common pattern. The most common HR-HPV genotype was HPV16, followed by HPV52, HPV58, HPV53 and HPV39. The most common low-risk HPV (LR-HPV) genotype was HPV6, followed by HPV61, HPV81, HPV54 and HPV11. Conclusions HPV infection is common among women in northern Henan Province. The highest infection prevalence was found in women less than 20 years old. The 9-valent HPV vaccine for routine vaccination is strongly recommended in northern Henan Province.

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Prevalence of genital human papillomavirus DNA in a sample of senior school-aged women in the Australian Capital Territory

Sexual Health ◽

10.1071/sh05047 ◽

2006 ◽

Vol 3 (2) ◽

pp. 91 ◽

Cited By ~ 5

Author(s):

Elissa J. O'Keefe ◽

Anne Gardner ◽

Marian J Currie ◽

Suzanne Garland ◽

Sepehr Tabrizi ◽

...

Keyword(s):

Risk Factors ◽

High School ◽

Human Papillomavirus ◽

Young Women ◽

Hpv Infection ◽

Senior High School ◽

Sexually Active ◽

Australian Capital Territory ◽

Hpv Dna ◽

Australian Capital

Background: A strong association between persistent infection with oncogenic types of human papillomavirus (HPV) and cervical cancer is well established. Small numbers of international studies examining adolescent HPV infection and the risk factors associated are published, but there is currently no evidence on the prevalence and risk factors for HPV in an Australian, sexually active female adolescent population. Methods: To provide prevalence and risk factors for HPV in a female sexually active, senior high school population in the Australian Capital Territory (ACT), a convenience sample of 161, 16–19-year-old females attending a senior high school was evaluated. The sample formed part of a larger sample recruited for a study of sexually transmitted infections and blood-borne viruses in senior high school students. A clinical record was used to collect information about sexual and other risk behaviours, while self-collected vaginal swabs were tested for HPV DNA detection and genotyping using polymerase chain reaction. Results: The prevalence of HPV DNA in this sample overall was 11.2%, with multiple genotypes in 38%. No statistically significant associations were found between HPV DNA and the number of male partners, age of coitarche, time since first sexually active, condom use, smoking or alcohol intake. Conclusions: This is the first Australian study that has examined the prevalence and risk factors for genital HPV in this demographic group. The prevalence of HPV infection is slightly lower than reported in similar age groups overseas and is lower than other Australian studies in older women and those attending sexual health centres. Of HPV-positive young women, high-risk genotypes were found in over half, with more than one-third of HPV existing as multiple genotypes. Large community-based prevalence studies are needed to guide the development of recommendations for the vaccination of young women against HPV and to support other health promotion initiatives.

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Human papillomavirus genotype distribution and risk factors for infection in women from a small municipality in north east Brazil

International Journal of STD & AIDS ◽

10.1258/ijsa.2009.009199 ◽

2012 ◽

Vol 23 (9) ◽

pp. e5-e10 ◽

Cited By ~ 4

Author(s):

F A Oliveira ◽

V Ehrig ◽

K Lang ◽

J Heukelbach ◽

M Stoffler-Meilicke ◽

...

Keyword(s):

Human Papillomavirus ◽

Preventive Measure ◽

Hpv Infection ◽

Population Based ◽

Genotype Distribution ◽

Hpv Dna ◽

Small Municipality ◽

North East ◽

Hpv Genotypes ◽

Cervical Cancer Screening Programme

In order to assess the prevalence of human papillomavirus (HPV) infection, the HPV genotypes and factors associated with infection, we conducted a population-based survey in a small municipality in north east Brazil among women aged between 12 and 49 years. A questionnaire regarding socioeconomic variables, reproductive life and sexual behaviour was used, and women were examined gynaecologically, followed by collection of vaginal lavage with saline solution for HPV DNA determination. HPV DNA was detected by the Digene®SHARP SignalTM-System, and further genotyped by INNO-LiPA Genotyping System®. Of 579 women, HPV infection was present in 68 (prevalence: 11.7%; 95% CI: 9.3–14.7). The most common HPV types were 16, 31 and 74, each accounting for 14.7% of infections. Of all HPV-positive women, 35.3% showed multiple HPV genotypes. Variables independently associated with HPV infection were: ≥3 partners in life (adjusted OR [aOR]: 3.06; 95% CI: 1.68–5.60) and the use of oral contraception in the last 12 months (aOR: 2.39; 95% CI: 1.33–4.30). Previous participation in a cervical cancer screening programme was protective (aOR: 0.28; 95% CI: 0.13–0.60). HPV infection is common among women from rural Brazil, and HPV genotypes identified indicate that immunization could be an important preventive measure in this population.

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Prevalence and distribution of human papillomavirus genotypes among women attending gynecology clinics in northern Henan Province of China

Virology Journal ◽

10.1186/s12985-021-01732-8 ◽

2022 ◽

Vol 19 (1) ◽

Author(s):

Xiangpeng Wang ◽

Yuan Song ◽

Xiaofei Wei ◽

Guanyu Wang ◽

Ruili Sun ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

Henan Province ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Prevalence ◽

Specific Hybridization ◽

Current Prevalence ◽

Hpv Genotypes ◽

High Risk Hpv

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers, including vulva, vagina, penis, anus, or oropharynx. However, in China's northern Henan Province, data on the prevalence and genotype distribution of HPV among women attending gynecology clinics is limited. This study aimed to investigate the current prevalence and genotype distribution of HPV among women attending gynecology clinics in northern Henan Province. Methods This study included 15,616 women aged 16–81 years old who visited the Xinxiang central hospital's gynecology department between January 2018 and December 2019. HPV DNA was detected by a conventional PCR method followed by HPV type-specific hybridization, which was designed to detect 17 high-risk HPV (HR-HPV) genotypes and 20 low-risk HPV (LR-HPV) genotypes. HPV prevalence and corresponding 95% confidence intervals (95% CI) were calculated using SPSS 18.0. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double, and multiple HPV infections accounted for 13.7%, 4.3%, and 1.8% of the total cases. Most infections were caused by HR-HPV (71.8%), and single genotype HPV infection (13.7%) was the most common pattern. The most common HR-HPV genotype was HPV16 (4.3%), followed by HPV52 (3.5%) and HPV58 (2.0%). The most common LR-HPV genotype was HPV6 (1.4%), followed by HPV61 (1.1%) and HPV81 (1.1%). Conclusions HPV infection is high among women attending gynecology clinics in northern Henan Province. The highest prevalence was found in women less than 20 years old. In northern Henan Province, the 9-valent HPV vaccine is strongly recommended for regular immunization.

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Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples

Virology Journal ◽

10.1186/s12985-021-01551-x ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Hana Jaworek ◽

Vladimira Koudelakova ◽

Ivana Oborna ◽

Blazena Zborilova ◽

Jana Brezinova ◽

...

Keyword(s):

Human Papillomavirus ◽

Hpv Infection ◽

Sexual Partners ◽

Fisher Exact Test ◽

Genotype Distribution ◽

Heterosexual Couples ◽

Anatomic Site ◽

Hpv Dna ◽

Hpv Test ◽

Penile Swabs

Abstract Background Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease. Methods Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195). Presence of HPV DNA in semen samples and cervical swabs was analyzed using the cobas® HPV Test and PapilloCheck®. Only the PapilloCheck® test was used to detect HPV in penile swabs. The genotype-specific prevalence and concordance of HPV infection not targeted by vaccine were evaluated using Fisher exact test. Results Both partners were infected with any HPV type in 13.8% (27/195) of couples and, of these couples, 55.6% (15/27) harbored at least one mutual genotype. High-risk HPV (hrHPV) genotypes were detected in 12.3% (24/195) of semen samples, 31.3% (61/195) of penile swabs, and 19.5% (38/195) of cervical swabs (P < 0.001). The most prevalent hrHPV genotype were HPV53 (2.56%; 5/195) in semen samples, HPV16 (6.67%, 13/195) in penile swabs and HPV39 (3.59%, 7/195) in cervical swabs. Low-risk (lrHPV) genotypes were detected in 5.13% (10/195) of semen samples, 15.9% (31/195) of penile swabs, and 4.10% (8/195) of cervical swabs (P < 0.001). Male sexual partners of HPV-positive women were more likely to be infected with at least one of the same HPV types than female sexual partners of HPV-positive men (34.9% vs. 17.9%, P = 0.055). Conclusions This study showed that the detection of HPV infection differ by anatomic site and gender. Regardless the anatomic site, high prevalence of HPV genital infection was found in both Czech men and women.

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The role of circumcision, tobacco, and alcohol use in genital human papillomavirus infection among men from Denmark

International Journal of STD & AIDS ◽

10.1177/09564624211014727 ◽

2021 ◽

pp. 095646242110147

Author(s):

Julie B Hebnes ◽

Christian Munk ◽

Kirsten Frederiksen ◽

Hans O Joergensen ◽

Thomas Iftner ◽

...

Keyword(s):

Risk Factors ◽

Sexual Behavior ◽

Human Papillomavirus ◽

Alcohol Use ◽

Potential Risk ◽

Hpv Infection ◽

Sex Partners ◽

Current Cigarette Smoking ◽

Hpv Dna ◽

Potential Risk Factors

It is crucial to understand the natural history of genital human papillomavirus (HPV) infection in men to prevent the increasing male HPV-related disease burden. We evaluated the associations between HPV infection and circumcision, smoking, and alcohol use after accounting for sexual behavior. The study included 2331 male personnel from Danish barracks. Penile swabs were tested for HPV DNA with a polymerase chain reaction assay, INNO-LiPA. All men completed a self-administered questionnaire providing data on potential risk factors for HPV such as lifestyle and sexual habits. Using multivariable logistic regression, associations between potential risk factors and HPV infection were estimated and expressed as odds ratios (ORs) with 95% confidence intervals (CI). Current cigarette smoking was associated with increased odds of HPV detection (OR = 1.2; 95% CI: 1.0–1.4), but we found no association with alcohol use in the analysis adjusted for sexual behavior. Circumcision reduced the odds of a prevalent HPV infection (OR = 0.7; 95% CI: 0.5–1.0) although not statistically significantly. Strong associations with lifetime and recent number of female sex partners were observed, but in contrast to uncircumcised men, increasing number of sex partners was not associated with higher HPV prevalence in circumcised men. In conclusion, smoking was associated with increased odds of penile HPV in men from the general population in Denmark, whereas circumcision seemed to reduce the risk. Moreover, our results indicated that there might be differences in the viral susceptibility between circumcised and uncircumcised men.

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HPV genotyping and risk factors for anal high-risk HPV infection in men who have sex with men from Toronto, Canada

Scientific Reports ◽

10.1038/s41598-021-84079-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yoojin Choi ◽

Mona Loutfy ◽

Robert S. Remis ◽

Juan Liu ◽

Anuradha Rebbapragada ◽

...

Keyword(s):

Risk Factors ◽

High Risk ◽

Hpv Vaccine ◽

Hpv Infection ◽

Categorical Variables ◽

Genotype Distribution ◽

Continuous Variables ◽

Chi Square ◽

Hpv Genotypes ◽

Sex With Men

AbstractMen who have sex with men (MSM) are disproportionately affected by anal cancer, predominantly caused by high-risk (HR) human papillomavirus (HPV) infection. Currently, the nonavalent HPV vaccine provides coverage against nine HPV genotypes, including seven HR-HPV genotypes. Here, we characterize anal HR-HPV genotype distribution and associated risk factors in MSM from Toronto, Canada recruited between September 2010 and June 2012. Wilcoxon–Mann–Whitney test was used for continuous variables, Chi-square test was performed for categorical variables, and a multivariable model using logistic regression was created to assess for correlates of anal HR-HPV infection. A total of 442 MSM were recruited, with a median age of 45 (IQR 38–50) and an overall HPV prevalence of 82%. The prevalence of any HR-HPV infection was 65.3% and 50.7% in the HIV-positive and HIV-negative MSM, respectively. No participant tested positive for all genotypes covered by the nonavalent vaccine. HIV status (aOR 1.806; 95% CI 1.159–2.816), smoking (aOR 2.176; 95% CI 1.285–3.685) and the number of lifetime sexual partners (aOR 2.466; 95% CI 1.092–5.567) were independent risk factors for anal HR-HPV infection. Our findings will be useful to inform HPV vaccine rollout and HPV prevention strategies in Canadian MSM.

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Distribution and Prevalence of High-Risk Human Papillomavirus Genotypes in Cervical Specimens

Flora the Journal of Infectious Diseases and Clinical Microbiology ◽

10.5578/flora.68958 ◽

2020 ◽

Vol 25 (3) ◽

pp. 325-331

Author(s):

Erkan Özmen ◽

Ülkü Altoparlak ◽

Muhammet Hamidullah Uyanık ◽

Abdulkadir Gülen

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Age Groups ◽

Hpv Infection ◽

High Rate ◽

Genotype Distribution ◽

Hpv Dna ◽

Hpv Test ◽

Significant Difference ◽

Hpv Types

Introduction: Human papillomavirus (HPV) is frequently a sexually transmitted virus and can cause cervical cancer in women. Cervical cancer is the second most common type of cancer among the developing countries. In this study, cervical HPV DNA positivity and genotype distributions were investigated in female patients living in our region and the results were compared with different studies. Materials and Methods: Between 1 July, 2017 and 1 March, 2019, 433 cervical swabs were sent to Ataturk University, Medical Faculty Hospital, Medical Microbiology Laboratory due to suspicion of HPV. Swab samples were evaluated for HPV virus using molecular (Polymerase Chain Reaction-PCR) methods. For this purpose, Xpert HPV Test (Cepheid, Inc, Sunnyvale, CA) was used to identify HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 t in a single sample. Results: Mean age of the patients ranged from 20 to 69 years, with a mean of 39.8 years (± 10.0). Positivity was detected in 62 of the 433 patients. Mean age of the positive patients was 40.2 years (± 11.3). When the positive patients were examined in terms of HPV types, the presence of HPV 16 was observed with a rate of 25.6%, while the HPV 18/45 types were found to be 9.0% in total. When patients were evaluated according to age groups, HPV DNA positivity was highest in the 25-34 age group with 38.7%. In our statistical study, there was no significant difference in HPV DNA positivity rate between the ages of 35 and under 35 years. Conclusion: This study demonstrates the prevalence and viral genotype distribution of HPV infection in women in Erzurum region. HPV type 16 is seen with a high rate in our region.

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Prevalence and genotype distribution of HPV infection among 214,715 women from Southern China, 2012-2018: baseline measures prior to mass HPV vaccination

10.21203/rs.3.rs-20658/v2 ◽

2020 ◽

Author(s):

Lipei Luo ◽

Ping He ◽

qiaotu liu ◽

Yanghua jiang ◽

Yang-nan Zhang ◽

...

Keyword(s):

Young Women ◽

Southern China ◽

Cervical Screening ◽

Hpv Vaccination ◽

Hpv Infection ◽

Cross Sectional Study ◽

Genotype Distribution ◽

Cross Sectional ◽

Hpv Prevalence ◽

Hpv Genotypes

Abstract Background: The epidemiology on the human papillomavirus (HPV) among females in Southern China is not well-established. Baseline data on the prevalence of HPV infection in China prior to mass prophylactic HPV vaccination would be useful. Thus, this study aimed to determine the type-specific HPV prevalence and distribution among females from Southern China prior to mass HPV vaccination. Methods: A retrospective cross-sectional study employing 214,715 women attending ChenZhou NO.1 People’s Hospital for cervical screening during 2012-2018 was conducted prior to widespread HPV vaccination. HPV genotype was detected using nucleic acid molecular diversion hybridization tests. The overall prevalence, age-specific prevalence, type distribution, and annual trend were analyzed. Results: The overall HPV prevalence was 18.71% (95% confidence interval [CI], 18.55%-18.88%) among Southern China females. During 2012–2018, the prevalence of HPV infection showed a downward tendency, from 21.63% (95% CI, 21.07%-22.20%) in 2012 to 18.75% (95% CI, 18.35%-19.16%) in 2018. Age-specific HPV distribution displayed a peak at young women aged less than 21 years (33.11%, 95% CI, 31.13%-35.15%), 20.07% (95% CI, 19.70%-20.44%) among women aged 21-30 years, 17.29% (95% CI, 17.01%-17.57%) among women aged 31-40 years, 17.23% (95% CI, 16.95%-17.51%) among women aged 41-50 years, 21.65% (95% CI, 21.11%-22.20%) among women aged 51-60 years, and 25.95% (95% CI, 24.86%-27.07%) among women aged over 60 years. Of the 21 subtypes identified, the most three prevalent high-risk HPV (HR-HPV) genotypes were HPV52 (5.12%; 95% CI, 21.11%-22.20%), -16 (2.96%; 95% CI, 2.89%-3.03%), and -58 (2.51%; 95% CI, 2.44%-2.58%); the predominant low-risk HPV (LR-HPV) genotypes were HPV81 (1.86%; 95%CI, 1.80%-1.92%) and -6 (0.69%; 95% CI, 0.66%-0.73%) respectively. Incidence of HR-HPV only, LR-HPV only and mixed LR- and HR-HPV were 15.17%, 2.07% and 1.47% respectively. Besides, single HPV infection accounted for 77.30% of all positive cases in this study.Conclusions: This study highlights 1) a high prevalence of HPV infection among females with a decreasing tendency towards 2012-2018, especially for young women under the age of 21 prior to mass HPV vaccination; 2) HPV52, -16 and -58 were the predominant HPV genotypes, suggesting potential use of HPV vaccine covering these HPV genotypes in Southern China.

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Age factor and implication of human papillomavirus type-specific prevalence in women with normal cervical cytology

Epidemiology and Infection ◽

10.1017/s0950268811000720 ◽

2011 ◽

Vol 140 (3) ◽

pp. 466-473 ◽

Cited By ~ 6

Author(s):

C. H. LAI ◽

A. CHAO ◽

C. J. CHANG ◽

C. C. HUANG ◽

L. C. WANG ◽

...

Keyword(s):

Human Papillomavirus ◽

Hormone Replacement ◽

Menopausal Status ◽

Hpv Infection ◽

Population Based ◽

Cervical Cytology ◽

Genotype Distribution ◽

Abnormal Cytology ◽

Normal Cytology ◽

Normal Cervical Cytology

SUMMARYThe prevalence and genotype distribution of human papillomavirus (HPV) infection in women with normal cervical cytology varies widely according to the population studied. Two non-overlapping population-based cohort studies of women aged ⩾30 years for the periods 2008–2009 (n=5026) and 2004–2005 (n=10 014) were analysed. The prevalence rate of HPV was 11·0% (95% CI 10·5–11·6). HPV infection was significantly associated with age, menopausal status, and inversely associated with hormone replacement therapy. There was an increasing trend of α3/α15, α5/α6, and multiple HPV infections with increasing age. The five most common types were HPV52, 18, 53, 58 and 70, while HPV16, 31, 33 ranked 21st, 25th, and 16th, respectively, in the merged cohort with normal cytology (n=14 724). HPV16, 31, and 33 were significantly associated with abnormal cytology, which could have resulted in their rarity in the total merged cohort (n=15 040).

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