Development of an integrated approach for comparison of in vitro and in vivo responses to particulate matter

Syzygium fruticosum (SF), a valuable Bangladeshi fruit, is considered an alternative therapeutic agent. Mainly, seeds are used as nutritional phytotherapy to ease physical and mental status by preventing chronic diseases. Here, we scrutinized the S. fruticosum seed’s fundamental importance in traditional medicine by following an integrated approach combining in vivo, in vitro, and in silico studies. The SF was fractionated with different solvents, and the ethyl acetate fraction of SF (EaF-SF) was further studied. Mice treated with EaF-SF (200 and 400 mg/kg) manifested anxiolysis evidenced by higher exploration in elevated plus maze and hole board tests. Similarly, a dose-dependent drop of immobility time in a forced swimming test ensured significant anti-depressant activity. Moreover, higher dose treatment exposed reduced exploratory behaviour resembling decreased movement and prolonged sleeping latency with a quick onset of sleep during the open field and thiopental-induced sleeping tests, respectively. In parallel, EaF-SF significantly (p < 0.001) and dose-dependently suppressed acetic acid and formalin-induced pain in mice. Also, a noteworthy anti-inflammatory activity and a substantial (p < 0.01) clot lysis activity (thrombolytic) was observed. Gas chromatography-mass spectrometry (GC–MS) analysis resulted in 49 bioactive compounds. Among them, 12 bioactive compounds with Lipinski’s rule and safety confirmation showed strong binding affinity (molecular docking) against the receptors of each model used. To conclude, the S. fruticosum seed is a prospective source of health-promoting effects that can be an excellent candidate for preventing degenerative diseases.

Download Full-text

Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo

Toxicology Reports ◽

10.1016/j.toxrep.2021.02.005 ◽

2021 ◽

Vol 8 ◽

pp. 349-358

Author(s):

Yu-Lin Dai ◽

Yun-Fei Jiang ◽

Yu-An Lu ◽

Jiang-Bo Yu ◽

Min-Cheol Kang ◽

...

Keyword(s):

Particulate Matter

Download Full-text

Integrated Approach of In Vivo and In Vitro Evaluation of the Involvement of Hepatic Uptake Organic Anion Transporters in the Drug Disposition in Rats Using Rifampicin as an Inhibitor

Drug Metabolism and Disposition ◽

10.1124/dmd.113.051052 ◽

2013 ◽

Vol 41 (7) ◽

pp. 1442-1449 ◽

Cited By ~ 15

Author(s):

Tomoki Imaoka ◽

Tsuyoshi Mikkaichi ◽

Koji Abe ◽

Masakazu Hirouchi ◽

Noriko Okudaira ◽

...

Keyword(s):

Drug Disposition ◽

Organic Anion ◽

Integrated Approach ◽

Hepatic Uptake ◽

Organic Anion Transporters ◽

In Vitro Evaluation ◽

Anion Transporters

Download Full-text

An integrated approach to unravel a crucial structural property required for the function of the insect steroidogenic Halloween protein Noppera-bo

Journal of Biological Chemistry ◽

10.1074/jbc.ra119.011463 ◽

2020 ◽

Vol 295 (20) ◽

pp. 7154-7167 ◽

Cited By ~ 1

Author(s):

Kotaro Koiwai ◽

Kazue Inaba ◽

Kana Morohashi ◽

Sora Enya ◽

Reina Arai ◽

...

Keyword(s):

Structural Characteristics ◽

Integrated Approach ◽

Binding Pocket ◽

Loss Of Function ◽

Tertiary Structures ◽

Ecdysteroid Biosynthesis ◽

17Β Estradiol ◽

Unique Amino Acid

Ecdysteroids are the principal steroid hormones essential for insect development and physiology. In the last 18 years, several enzymes responsible for ecdysteroid biosynthesis encoded by Halloween genes were identified and genetically and biochemically characterized. However, the tertiary structures of these proteins have not yet been characterized. Here, we report the results of an integrated series of in silico, in vitro, and in vivo analyses of the Halloween GST protein Noppera-bo (Nobo). We determined crystal structures of Drosophila melanogaster Nobo (DmNobo) complexed with GSH and 17β-estradiol, a DmNobo inhibitor. 17β-Estradiol almost fully occupied the putative ligand-binding pocket and a prominent hydrogen bond formed between 17β-estradiol and Asp-113 of DmNobo. We found that Asp-113 is essential for 17β-estradiol–mediated inhibition of DmNobo enzymatic activity, as 17β-estradiol did not inhibit and physically interacted less with the D113A DmNobo variant. Asp-113 is highly conserved among Nobo proteins, but not among other GSTs, implying that this residue is important for endogenous Nobo function. Indeed, a homozygous nobo allele with the D113A substitution exhibited embryonic lethality and an undifferentiated cuticle structure, a phenocopy of complete loss-of-function nobo homozygotes. These results suggest that the nobo family of GST proteins has acquired a unique amino acid residue that appears to be essential for binding an endogenous sterol substrate to regulate ecdysteroid biosynthesis. To the best of our knowledge, ours is the first study describing the structural characteristics of insect steroidogenic Halloween proteins. Our findings provide insights relevant for applied entomology to develop insecticides that specifically inhibit ecdysteroid biosynthesis.

Download Full-text

Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2014.04.029 ◽

2014 ◽

Vol 274 ◽

pp. 384-391 ◽

Cited By ~ 18

Author(s):

Kang-Yun Lee ◽

Chris Kong-Chu Wong ◽

Kai-Jen Chuang ◽

Mauo-Ying Bien ◽

Jun-Ji Cao ◽

...

Keyword(s):

Particulate Matter ◽

In Vivo Study ◽

Methionine Oxidation

Download Full-text

Efficacy, Mechanism, and Safety of Herbal Medicine for Respiratory Disease Induced by Particulate Matter: a Protocol for a Scoping Review

10.21203/rs.3.rs-295016/v1 ◽

2021 ◽

Author(s):

Jungtae Leem ◽

Yohwan Kim ◽

Kwan-Il Kim

Keyword(s):

Particulate Matter ◽

Animal Models ◽

Respiratory Disease ◽

Scoping Review ◽

Respiratory Diseases ◽

English Language ◽

Herbal Medicines ◽

Cochrane Central Register

Abstract Background: Particulate matter (PM) is an important environmental risk factor in the initiation and exacerbation of respiratory disease. Various herbal medicines have exhibited a reduction in symptoms of respiratory diseases induced by PM in animal models; however, their efficacy, mechanism, and safety have not been reviewed. This review will evaluate the efficacy, safety, and mechanism of action of herbal medicines in respiratory diseases caused by PM. Methods:We will follow the scoping review framework developed by Arksey and O’Malley. MEDLINE (via PubMed), EMBASE, and the Cochrane Central Register of Controlled Trials will be searched for relevant English-language publications, and only peer-reviewed, controlled comparative in-vivo/in-vitro studies examining the effects of herbs in animal models of respiratory disease induced by PM will be included. The basic characteristics, research method, possible mechanism, and results will be extracted. The primary outcome will be pulmonary function; secondary outcomes will be inflammatory markers, reactive oxygen species, histology and mechanisms, and adverse events. Two researchers will independently perform the study selection, data extraction, and quality assessment. RevMan software (version 5.3) will be used for the quantitative data synthesis. When appropriate, data will be pooled for meta-analysis using fixed or random effects models; otherwise, evidence will be summarized qualitatively. Ethics and Dissemination: Ethical approval is not required because individual patient data will not be included. The findings will be disseminated through peer-reviewed publications or conference presentations.Registration number: This review protocol has been registered with the Open Science Framework on February 12, 2021 (https://osf.io/s7uvk/)

Download Full-text

Efficacy of Potential Control Agents Against Rosellinia necatrix and Their Physiological Impact on Avocado

Plant Disease ◽

10.1094/pdis-08-20-1751-re ◽

2021 ◽

Author(s):

Phinda Magagula ◽

Nicky Taylor ◽

Velushka Swart ◽

Noëlani van den Berg

Keyword(s):

Integrated Management ◽

Integrated Approach ◽

Dry Mass ◽

Persea Americana ◽

Effective Control ◽

Rosellinia Necatrix ◽

Potential Control ◽

Greenhouse Trial

Rosellinia necatrix is the causal agent of white root rot (WRR), a fatal disease affecting many woody plants, including avocado (Persea americana). As with other root diseases, an integrated approach is required to control WRR. No fully effective control methods are available, and no chemical or biological agents against R. necatrix have been registered for use on avocado in South Africa. Fluazinam has shown promising results in the greenhouse and field in other countries, including Spain. The current study aimed to investigate the potential of a fumigant, chloropicrin, and biological control agents (B-Rus, Beta-Bak, Mity-Gro, and Trichoderma) against R. necatrix both in vitro and in vivo as compared with fluazinam. In a greenhouse trial, results showed that Trichoderma and B-Rus were as effective as fluazinam at inhibiting R. necatrix in vitro and suppressed WRR symptoms when applied before inoculation with R. necatrix. In contrast, Mity-Gro and Beta-Bak failed to inhibit the pathogen in vitro and in the greenhouse trial, despite application of the products to plants before R. necatrix infection. Fluazinam suppressed WRR symptoms in plants when applied at the early stages of infection, whereas chloropicrin rendered the pathogen nonviable when used as a preplant treatment. Plants treated with Trichoderma, B-Rus, and fluazinam sustained dry mass production and net CO2 assimilation by maintaining the green leaf tissues despite being infected with the pathogen. This study has important implications for the integrated management of WRR.

Download Full-text

How Harmful Is Particulate Matter Emitted from Biomass Burning? A Thailand Perspective

Current Pollution Reports ◽

10.1007/s40726-019-00125-4 ◽

2019 ◽

Vol 5 (4) ◽

pp. 353-377 ◽

Cited By ~ 13

Author(s):

Helinor J. Johnston ◽

William Mueller ◽

Susanne Steinle ◽

Sotiris Vardoulakis ◽

Kraichat Tantrakarnapa ◽

...

Keyword(s):

Particulate Matter ◽

Health Effects ◽

Biomass Burning ◽

Large Body ◽

Experimental Studies ◽

In Vivo Studies ◽

Indoor And Outdoor Environments ◽

Epidemiology Studies

Abstract Purpose of Review A large body of epidemiological evidence demonstrates that exposure to particulate matter (PM) is associated with increased morbidity and mortality. Many epidemiology studies have investigated the health effects of PM in Europe and North America and focussed on traffic derived PM. However, elevated levels of PM are a global problem and the impacts of other sources of PM on health should be assessed. Biomass burning can increase PM levels in urban and rural indoor and outdoor environments in developed and developing countries. We aim to identify whether the health effects of traffic and biomass burning derived PM are similar by performing a narrative literature review. We focus on Thailand as haze episodes from agricultural biomass burning can substantially increase PM levels. Recent Findings Existing epidemiology, in vitro and in vivo studies suggest that biomass burning derived PM elicits toxicity via stimulation of oxidative stress, inflammation and genotoxicity. Thus, it is likely to cause similar adverse health outcomes to traffic PM, which causes toxicity via similar mechanisms. However, there is conflicting evidence regarding whether traffic or biomass burning derived PM is most hazardous. Also, there is evidence that PM released from different biomass sources varies in its toxic potency. Summary We recommend that epidemiology studies are performed in Thailand to better understand the impacts of PM emitted from specific biomass sources (e.g. agricultural burning). Further, experimental studies should assess the toxicity of PM emitted from more diverse biomass sources. This will fill knowledge gaps and inform evidence-based interventions that protect human health.

Download Full-text