Correction to: Liver cancer cell lines distinctly mimic the metabolic gene expression pattern of the corresponding human tumours

Abstract Background：Hepatocellular carcinoma (HCC) is a common cancer and the leading cause is persistent hepatitis B virus infection. We aimed to identify some core genes and pathways for HBV-related HCC. Methods: Gene expression profiles of GSE62232, GSE121248, and GSE94660 were available from Gene Expression Omnibus. The GEO2R online tool and Venn diagram software were applied to analyze commonly differentially expressed genes. Then functional enrichment analysis using Gene Ontology (GO) and the Kyoto Encyclopedia of Gene and Genome (KEGG) as well as the protein-protein interaction (PPI) network was conducted. The overall survival rates and the expression levels were detected by Kaplan-Meier plotter and Gene Expression Profiling Interactive Analysis (GEPIA). Next, gene set enrichment analysis (GSEA) was performed to verify the KEGG pathway analysis. Furthermore, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to validate the levels of gene expression in tumor tissues from HBV related HCC patients, HBV-related liver cell lines, and transfection si-p53 and knock-out p53 liver cancer cell lines. Finally, the prediction of the ceRNA network was constructed with R software. Results: Fifteen highly expressed genes associated with significantly worse prognoses were selected and CCNB1, CDK1, and RRM2 in the p53 signaling pathway were identified as core genes. GSEA results showed highly-expressed samples of three core genes were all enriched in the p53 signaling pathway in a validation dataset(P<0.0001). Expression of these three core genes were consistently higher in tumor tissue samples (P<0.0001) and liver cancer cell lines (P<0.05). However, transfection si-p53 and knock-out p53 liver cancer cell lines had lower expression (P<0.05). LncRNAs, including NEAT1, MALAT1, XIST, AC021078.1, and SNHG16, were identified by close interactions with core genes. Conclusions: CCNB1, CDK1, and RRM2 were enriched in the p53 signaling pathway and could be potential biomarkers and therapeutic targets for HBV-related HCC.

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Gene expression profiles of liver cancer cell lines reveal two hepatocyte-like and fibroblast-like clusters

PLoS ONE ◽

10.1371/journal.pone.0245939 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0245939

Author(s):

Keita Fukuyama ◽

Masataka Asagiri ◽

Masahiro Sugimoto ◽

Hiraki Tsushima ◽

Satoru Seo ◽

...

Keyword(s):

Gene Expression ◽

Liver Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Cancer Cell Lines ◽

Liver Cancer Cell ◽

Study Objective

Cancer cell lines are widely used in basic research to study cancer development, growth, invasion, or metastasis. They are also used for the development and screening of anticancer drugs. However, there are no clear criteria for choosing the most suitable cell lines among the wide variety of cancer cell lines commercially available for research, and the choice is often based on previously published reports. Here, we investigated the characteristics of liver cancer cell lines by analyzing the gene expression data available in the Cancer Cell Line Encyclopedia. Unsupervised clustering analysis of 28 liver cancer cell lines yielded two main clusters. One cluster showed a gene expression pattern similar to that of hepatocytes, and the other showed a pattern similar to that of fibroblasts. Analysis of hepatocellular carcinoma gene expression profiles available in The Cancer Genome Atlas showed that the gene expression patterns in most hepatoma tissues were similar to those in the hepatocyte-like cluster. With respect to liver cancer research, our findings may be useful for selecting an appropriate cell line for a specific study objective. Furthermore, our approach of utilizing a public database for comparing the properties of cell lines could be an attractive cell line selection strategy that can be applied to other fields of research.

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Exploring molecular basis of therapy response in patient derived primary liver cancer cell lines

10.1055/s-0038-1677199 ◽

2019 ◽

Author(s):

D Castven ◽

D Becker ◽

C Czauderna ◽

D Wilhelm ◽

JB Andersen ◽

...

Keyword(s):

Liver Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Molecular Basis ◽

Primary Liver Cancer ◽

Therapy Response ◽

Cancer Cell Lines ◽

Liver Cancer Cell

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Underutilized Mexican Plants: Screening of Antioxidant and Antiproliferative Properties of Mexican Cactus Fruit Juices

Plants ◽

10.3390/plants10020368 ◽

2021 ◽

Vol 10 (2) ◽

pp. 368

Author(s):

Elda M. Melchor Martínez ◽

Luisaldo Sandate-Flores ◽

José Rodríguez-Rodríguez ◽

Magdalena Rostro-Alanis ◽

Lizeth Parra-Arroyo ◽

...

Keyword(s):

Cell Proliferation ◽

Liver Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

In Vitro Assays ◽

Total Phenolic ◽

Liver Cancer Cell ◽

Antiproliferative Activities

Cacti fruits are known to possess antioxidant and antiproliferative activities among other health benefits. The following paper evaluated the antioxidant capacity and bioactivity of five clarified juices from different cacti fruits (Stenocereus spp., Opuntia spp. and M. geomettizans) on four cancer cell lines as well as one normal cell line. Their antioxidant compositions were measured by three different protocols. Their phenolic compositions were quantified through high performance liquid chromatography and the percentages of cell proliferation of fibroblasts as well as breast, prostate, colorectal, and liver cancer cell lines were evaluated though in vitro assays. The results were further processed by principal component analysis. The clarified juice from M. geomettizans fruit showed the highest concentration of total phenolic compounds and induced cell death in liver and colorectal cancer cells lines as well as fibroblasts. The clarified juice extracted from yellow Opuntia ficus-indica fruit displayed antioxidant activity as well as a selective cytotoxic effect on a liver cancer cell line with no toxic effect on fibroblasts. In conclusion, the work supplies evidence on the antioxidant and antiproliferative activities that cacti juices possess, presenting potential as cancer cell proliferation preventing agents.

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Specific Inhibition of the Redox Activity of Ape1/Ref-1 by E3330 Blocks Tnf-Α-Induced Activation of Il-8 Production in Liver Cancer Cell Lines

PLoS ONE ◽

10.1371/journal.pone.0070909 ◽

2013 ◽

Vol 8 (8) ◽

pp. e70909 ◽

Cited By ~ 24

Author(s):

Laura Cesaratto ◽

Erika Codarin ◽

Carlo Vascotto ◽

Antonio Leonardi ◽

Mark R. Kelley ◽

...

Keyword(s):

Liver Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Redox Activity ◽

Specific Inhibition ◽

Liver Cancer Cell ◽

Tnf Α

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Single-Cell Multiomics Analysis Reveals Heterogeneous Cell States Linked to Metastatic Potential in Liver Cancer Cell Lines

SSRN Electronic Journal ◽

10.2139/ssrn.3904960 ◽

2021 ◽

Author(s):

Shanshan Wang ◽

Jia-Rui Xie ◽

Xuanxuan Zou ◽

Taotao Pan ◽

Qi-Chao Yu ◽

...

Keyword(s):

Liver Cancer ◽

Single Cell ◽

Cell Lines ◽

Cancer Cell ◽

Metastatic Potential ◽

Cancer Cell Lines ◽

Liver Cancer Cell

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Anti-Proliferative Effects of an Extra-Virgin Olive Oil Extract Enriched in Ligstroside Aglycone and Oleocanthal on Human Liver Cancer Cell Lines

Cancers ◽

10.3390/cancers11111640 ◽

2019 ◽

Vol 11 (11) ◽

pp. 1640 ◽

Cited By ~ 5

Author(s):

De Stefanis ◽

Scimè ◽

Accomazzo ◽

Catti ◽

Occhipinti ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cancer ◽

Olive Oil ◽

Cell Lines ◽

Cancer Cell ◽

Virgin Olive Oil ◽

Cancer Cell Lines ◽

Liver Cancer Cell ◽

Antioxidant Power ◽

Polyphenolic Extract

Oleocanthal and ligstroside aglycone are olive oil-derived polyphenols. The former interferes with tumor growth with minor or no cytotoxicity on non-tumorigenic primary cell lines. The information about the bioactivity of ligstroside aglycone are scanty, with the exception of a known antioxidant power. Hepatocellular carcinoma is a malignant tumor with high mortality rates. Systemic chemotherapy is only marginally effective and is frequently complicated by toxicity. Previous observations have shown that hepatocellular carcinoma cell lines become more sensitive to taxol when it is combined with Tumor Necrosis Factor α (TNFα). The present work aimed to assess the effects of a polyphenolic extract containing both oleocanthal and ligstroside aglycone on proliferation and/or death in three liver cancer cell lines (HepG2, Huh7 and Hep3B). The possibility to enhance such effect by the addition of TNFα was also investigated. Both cell proliferation and death were enhanced by the exposure to the polyphenolic extract. Such effect was associated with induction of autophagy and could be potentiated by TNFα. The presence of ligstroside aglycone in the extract lowered the oleocanthal concentration required for cytotoxicity. These results show for the first time that the effects of a polyphenol extract can be potentiated by TNFα and that modulation of autophagy likely account for these effects.

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