scholarly journals Co-production of amino acid-rich xylooligosaccharide and single-cell protein from paper mulberry by autohydrolysis and fermentation technologies

2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Yang Gu ◽  
Yingying Hu ◽  
Caoxing Huang ◽  
Chenhuan Lai ◽  
Zhe Ling ◽  
...  

Abstract Background Autohydrolysis is an extensively investigated pretreatment method due to its environmental friendliness. During autohydrolysis, most xylan from hemicellulose can be converted into xylooligosaccharides (XOS), and cellulose in the autohydrolyzed residues can be transformed into glucose after enzymatic hydrolysis. Both of these are value-added biochemicals in the biorefining process. In this work, paper mulberry (PM), which contains abundant protein, was utilized as a raw material to coproduce XOS and single-cell protein (SCP) through autohydrolysis and fermentation technologies. Results The results showed that 8.3 g of XOS and 1.8 g of amino acids could be recovered in the autohydrolysate (based on 100 g raw material) after autohydrolysis (170 °C, 1 h). Moreover, 5.7 g of low-DP XOS along with 1.8 g of amino acids could be further obtained from the autohydrolysate after hydrolysis with endo-β-1-4-xylanase. In addition, 20.1 g of fermentable monosaccharides was recovered after hydrolyzing the autohydrolyzed PM with cellulase, which can be used to produce 4.8 g of SCP after fermentation with Candida utilis. Conclusion As a valuable application of PM, a novel process is proposed to coproduce amino acid-rich XOS and SCP through autohydrolysis. The carbohydrate of PM is effectively converted to high value-added products.

2018 ◽  
Vol 17 (23) ◽  
pp. 716-723 ◽  
Author(s):  
Kounbesioune SOMDA Marius ◽  
NIKIEMA Mahamadi ◽  
KEITA Ibrahim ◽  
MOGMENGA Iliassou ◽  
H. S. KOUHOUNDE Sonagnon ◽  
...  

The following topics are covered in this paper: the European feedstuff business, size, shape and source; the protein and amino acid requirements of the principal target species; the place of single cell protein (s.c.p.); the raw material options and the technical challenge of large scale s.c.p. manufacture; fermentation of s.c.p., its stoichiometry, mass and heat transfer requirements; static and dynamic optimization and control; the pressure cycle fermenter; the principle of sterility and the engineering design constraints; the nutritional performance of I.C.I.’s ‘Pruteen’ and the future for s.c.p.


Author(s):  
Mridul Umesh ◽  
Thazeem Basheer

Biosynthetic capabilities of microbes have solved several hurdles in the human welfare. Microbes have served and continue to serve as imperial candidates in both production and management strategies. Microbe mediated techniques has emerged as ecofriendly and sustainable alternative to their synthetic counterparts. Fruit based industries produces large volumes of solid and liquid wastes contributing to increase in pollution load. Disposal of these waste not only represent loss of valuable biomass but also leads to substantial increase in Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD). However, in spite of their pollution and hazard aspects, in many cases, fruit processing wastes have a promising potential for being chief raw materials for secondary industries. This chapter summarizes microbe mediated fermentative utilization of fruit waste, for the production of value added products like organic acid, single cell protein, bioplastics, enzymes and biogas.


2019 ◽  
Vol 102 (11) ◽  
pp. 9749-9762 ◽  
Author(s):  
H.M. Buitrago Mora ◽  
M.A. Piñeros ◽  
D. Espinosa Moreno ◽  
S. Restrepo Restrepo ◽  
J.E.C. Cardona Jaramillo ◽  
...  

1975 ◽  
Vol 38 (9) ◽  
pp. 521-526 ◽  
Author(s):  
M. D. McELWAIN ◽  
T. RICHARDSON ◽  
C. H. AMUNDSON

Single cell protein concentrate was prepared by extracting dried Candida utilis with 0.2 N NaOH at 95 C for 10 min. The soluble protein was precipitated at pH 3.5, washed, and lyophilized. Portions of the protein isolates were succinylated so that 84% of the free amino groups were blocked. Alkaline extractions under optimum conditions gave a yield of 25 to 30% of total solids. Nucleic acid content of the isolates was 10.4% whereas protein comprised 67.3%. Succinylated (SI) and nonsuccinylated (NSI) single cell protein concentrate exhibited similar solubilities above pH 4, the apparent isoelectric point. At pH values below 4, SI was quite insoluble whereas NSI was very soluble at pH 2. Digestibilities as measured by percentage of lysine released from the protein by pepsin and pancreatin were 32.7%, 18.6%, and 3.3% for NSI, whole egg, and SI, respectively. Stabilities of emulsions prepared from corn oil (20–50%) tended to be lower when gelatin was used at the 1% level compared to NSI and SI. However, higher levels of gelatin tended to yield more stable emulsions compared to NSI and SI. Stabilities of emulsions prepared from NSI and SI tended to be comparable with NSI being slightly better. Viscosities of emulsions increased with increasing oil content and increasing emulsifier concentration.


1982 ◽  
Vol 61 (2) ◽  
pp. 337-344 ◽  
Author(s):  
N.J. DAGHIR ◽  
J.L. SELL

1991 ◽  
Vol 74 (1) ◽  
pp. 104-106
Author(s):  
Abdul Aziz ◽  
Faisal Abu-Dagga

Abstract A commercially available single-cell protein (Pruteen) was analyzed for amino acids, fatty acids, and major, minor, and trace-element composition after storage at room temperature and at 4°C over a period of 5 years. The purpose was to assess its suitability as an organic-nutrient standard reference material for food analysis. The material showed longterm stability and is, therefore, recommended for interlaboratory certification studies.


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