scholarly journals Transcriptome profiling of posterior kidney of brown trout, Salmo trutta, during proliferative kidney disease

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Arun Sudhagar ◽  
Reinhard Ertl ◽  
Gokhlesh Kumar ◽  
Mansour El-Matbouli
Keyword(s):  
Immune System ◽  
Kidney Disease ◽  
Differentially Expressed Genes ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Proliferative Kidney Disease ◽  
Immune System Process ◽  
Upregulated Genes

Abstract Background Tetracapsuloides bryosalmonae is a myxozoan parasite which causes economically important and emerging proliferative kidney disease (PKD) in salmonids. Brown trout, Salmo trutta is a native fish species of Europe, which acts as asymptomatic carriers for T. bryosalmonae. There is only limited information on the molecular mechanism involved in the kidney of brown trout during T. bryosalmonae development. We employed RNA sequencing (RNA-seq) to investigate the global transcriptome changes in the posterior kidney of brown trout during T. bryosalmonae development. Methods Brown trout were exposed to the spores of T. bryosalmonae and posterior kidneys were collected from both exposed and unexposed control fish. cDNA libraries were prepared from the posterior kidney and sequenced. Bioinformatics analysis was performed using standard pipeline of quality control, reference mapping, differential expression analysis, gene ontology, and pathway analysis. Quantitative real time PCR was performed to validate the transcriptional regulation of differentially expressed genes, and their correlation with RNA-seq data was statistically analyzed. Results Transcriptome analysis identified 1169 differentially expressed genes in the posterior kidney of brown trout, out of which 864 genes (74%) were upregulated and 305 genes (26%) were downregulated. The upregulated genes were associated with the regulation of immune system process, vesicle-mediated transport, leucocyte activation, and transport, whereas the downregulated genes were associated with endopeptidase regulatory activity, phosphatidylcholine biosynthetic process, connective tissue development, and collagen catabolic process. Conclusion To our knowledge, this is the first RNA-seq based transcriptome study performed in the posterior kidney of brown trout during active T. bryosalmonae development. Most of the upregulated genes were associated with the immune system process, whereas the downregulated genes were associated with other metabolic functions. The findings of this study provide insights on the immune responses mounted by the brown trout on the developing parasite, and the host molecular machineries modulated by the parasite for its successful multiplication and release.

Suppressed Immune-Related Profile Rescues Abortion-Prone Fetuses: A Novel Insight Into the CBA/J × DBA/2J Mouse Model

2019 ◽  
Vol 26 (11) ◽  
pp. 1485-1492
Author(s):  
Xiaochun Yi ◽  
Jie Zhang ◽  
Huixiang Liu ◽  
Tianxia Yi ◽  
Yuhua Ou ◽  
...  
Keyword(s):  
Immune System ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Poor Treatment ◽  
Polymerase Chain ◽  
Poor Treatment Outcome ◽  
Immune Related Genes ◽  
And Control ◽  
Upregulated Genes ◽  
Tgf Β1

The adverse clinical result and poor treatment outcome in recurrent spontaneous abortion (RSA) make it necessary to understand the pathogenic mechanism. The mating combination CBA/J × DBA/2 has been widely used as an abortion-prone model compared to DBA/2-mated CBA/J mice. Here, we used RNA-seq to get a comprehensive catalogue of genes differentially expressed between survival placenta in abortion-prone model and control. Five hundred twenty-four differentially expressed genes were obtained followed by clustering analysis, Gene Ontology analysis, and pathway analysis. We paid more attention to immune-related genes namely “immune response” and “immune system process” including 33 downregulated genes and 28 upregulated genes. Twenty-one genes contribute to suppressing immune system and 7 are against it. Six genes were validated by reverse transcription-polymerase chain reaction, namely Ccr1l1, Tlr4, Tgf-β1, Tyro3, Gzmb, and Il-1β. Furthermore, Tlr4, Tgf-β1, and Il-1β were analyzed by Western blot. Such immune profile gives us a better understanding of the complicated immune processing in RSA and immunosuppression can rescue pregnancy loss.

scholarly journals A brown trout ( Salmo trutta ) population faces devastating consequences due to proliferative kidney disease and temperature increase: A case study from Austria

2019 ◽  
Vol 29 (3) ◽  
pp. 465-476 ◽  
Author(s):  
Karoline Waldner ◽  
Thomas Bechter ◽  
Stefan Auer ◽  
Florian Borgwardt ◽  
Mansour El‐Matbouli ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Brown Trout ◽  
Temperature Increase ◽  
Salmo Trutta ◽  
Proliferative Kidney Disease ◽  
Brown Trout Salmo Trutta

Temperature-related parasite infection dynamics: the case of proliferative kidney disease of brown trout

Parasitology ◽  
2017 ◽  
Vol 145 (3) ◽  
pp. 281-291 ◽  
Author(s):  
NICOLE STREPPARAVA ◽  
HELMUT SEGNER ◽  
ALBERT ROS ◽  
HANNA HARTIKAINEN ◽  
HEIKE SCHMIDT-POSTHAUS ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Infection Intensity ◽  
Fish Host ◽  
Parasite Development ◽  
Parasite Transmission ◽  
Wildlife Diseases ◽  
Proliferative Kidney Disease ◽  
Transmission Potential

SUMMARYClimate change, in particular rising temperature, is suspected to be a major driver for the emergence of many wildlife diseases. Proliferative kidney disease of salmonids, caused by the myxozoanTetracapsuloides bryosalmonae, was used to evaluate how temperature dependence of host–parasite interactions modulates disease emergence. Brown trout (Salmo trutta fario) kept at 12 and 15 °C, were experimentally infected withT. bryosalmonae. Parasite development in the fish host and release of spores were quantified simultaneously to unravel parasite transmission potential from the vertebrate to the invertebrate host. A change to a stable plateau in infection intensity of the kidney coincided with a threshold at which spore shedding commenced. This onset of parasite release was delayed at the low temperature in accordance with reaching this infection intensity threshold, but the amount of spores released was irrespective of temperature. The production of parasite transmission stages declined with time. In conclusion, elevated temperature modifies the parasite transmission opportunities by increasing the duration of transmission stage production, which may affect the spread and establishment of the parasite in a wider range of rivers.

scholarly journals Kinetics of Parasite-Specific Antibody and B-Cell-Associated Gene Expression in Brown Trout, Salmo trutta during Proliferative Kidney Disease

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1244
Author(s):  
Saloni Shivam ◽  
Mansour El-Matbouli ◽  
Gokhlesh Kumar
Keyword(s):  
Kidney Disease ◽  
B Cell ◽  
Brown Trout ◽  
Specific Antibody ◽  
Salmo Trutta ◽  
Time Course ◽  
Serum Antibody ◽  
Chronic Infections ◽  
Proliferative Kidney Disease ◽  
Time Points

Tetracapsuloides bryosalmonae, a myxozoan endoparasite often causes chronic infection in brown trout. Antiparasite immunity mediated by antibodies and B cells is known as an important determinant of host survival and parasite proliferation during chronic infections. Accordingly, studying their time course during proliferative kidney disease (PKD) might be helpful in improving our understanding of its chronic nature. Therefore, we conducted this study to examine parasite specific serum antibody and B-cell-mediated response in laboratory-infected brown trout at different time points. Brown trout were exposed to the spores of T. bryosalmonae, derived from infected bryozoans. Samples were collected at different time points and processed for indirect ELISA, histopathology, and qRT-PCR. T. bryosalmonae specific antibody was detected at 4 weeks post exposure (wpe) and it persisted until 17 wpe. Additionally, the expressions of C4A, CD34, CD79A, BLNK, CD74, BCL7, and CD22 were differentially regulated in the important immune organs, kidney and spleen. To our knowledge, this is the first study addressing anti-T. bryosalmonae antibody response in brown trout at different time points. The results from this study provide valuable insights into the processes leading to changes in B cell development, inflammation and antibody production during the course of PKD in brown trout.

Effects of macrophage-conditioned medium on sika deer (Cervus nippon) antler stem cells

2020 ◽  
Vol 60 (10) ◽  
pp. 1326
Author(s):  
Zhen Wang ◽  
Datao Wang ◽  
Tao Qin ◽  
Hengxing Ba ◽  
Guanning Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Immune System ◽  
Conditioned Medium ◽  
Differentially Expressed Genes ◽  
Expression Profiles ◽  
Great Influence ◽  
Cervus Nippon ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Organ Regeneration

Context Immune system has been claimed as the ‘main switch’ of tissue or organ regeneration. Among immune cells, macrophages stand out as important modulators in mutiple regeneration models, such as planarian, axolotl, mammalian hair and liver. As a unique model for mammals, deer antler is considered to ideal for studying complete mammalian organ regeneration. Studies have found that antler regeneration is a stem cell-based process and antler stem cells locate in the pedicle periosteum (PP). Although the regulatory roles of the immune system in other regeneration models have been extensively studied, they remain unstudied in antler regeneration. Aims To explore the possible role of macrophages in the PP cells (PPCs). Methods We treated PPCs with a macrophage-conditioned medium (MCM) and detected effects of MCM on proliferation, migration and apoptosis of the PPCs, and identified differentially expressed genes by using the RNA-seq technique. Key results We found that MCM enhanced proliferation rate and migration rate significantly and stimulated apoptosis of the PPCs. Using the RNA-seq technique, we identified 112 differentially expressed genes in the PPCs (38 downregulated and 74 upregulated) after the MCM treatment. Furthermore, gene-ontology annotation analyses showed that the upregulated genes were mainly involved in cell adhesion, chemotaxis, wound healing, growth factor-stimulated responses, and bone formation, and the downregulated genes were involved in regulation of biosynthesis. Conclusions MCM had a great influence on the antler stem cells, and macrophages might regulate antler regeneration through altering the microenvironment and gene-expression profiles of the PPCs. Implications We believe that the results of the present study would facilitate the discovery of the roles of immune system in antler stem cells and, thus, mammalian organ regeneration in general.

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