Antimicrobial susceptibility profiles of Escherichia coli and Klebsiella pneumoniae isolated from outpatients in urban and rural districts of Uganda

ABSTRACT From 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates in European intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (P < 0.001), but not for ESBL-positive Klebsiella pneumoniae isolates (16.4% to 17.9% [P > 0.05]). Hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for E. coli (P < 0.001) and 20.9% versus 5.3%, respectively, for K. pneumoniae (P < 0.01). Carbapenems were consistently the most active drugs tested.

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Cloacal enterobacteria isolated from captive roadside hawks (Rupornis magnirostris, GMELIN, 1788) and their antimicrobial susceptibility profile

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/issn.1678-4456.v53i2p207-213 ◽

2016 ◽

Vol 53 (2) ◽

pp. 207 ◽

Cited By ~ 2

Author(s):

Ewerton Fylipe De Araújo Silva ◽

Joanna Francyne Silva de Barros ◽

Kleber Botelho Fraga ◽

Carolina Peixoto Magalhães ◽

José Eduardo Garcia ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Antimicrobial Susceptibility ◽

Klebsiella Oxytoca ◽

Macconkey Agar ◽

Salmonella Spp ◽

Antimicrobial Susceptibility Profile

O conhecimento das enterobactérias presentes em Gavião-carijó pode trazer uma compreensão sobre as doenças infecciosas que podem acometer essa ave, como também outros animais e/ou humanos, além de trazer mais informações sobre essa espécie de grande importância ecológica. Desta forma, o objetivo do estudo foi determinar as enterobactérias presentes na cloaca de Gaviões-carijós cativos e seu perfil de susceptibilidade a antimicrobianos. Inicialmente, foram coletadas amostras cloacais de nove espécimes com o auxílio de swabs. As amostras foram plaqueadas em ágar MacConkey, ágar Hektoen, ágar EMB e ágar SS e incubadas por 24 h a 35°C. Após incubação, as colônias foram submetidas às provas bioquímicas para confirmação da presença de enterobactérias. Posteriormente, o perfil de susceptibilidade das bactérias frente a agentes antimicrobianos foi avaliado através do teste da difusão em disco de acordo com o Clinical and Laboratory Standards Institute (CLSI). Escherichia coli (77,8%), Klebsiella oxytoca (11,1%), Klebsiella pneumoniae (11,1%) e Salmonella spp. (55,6%) foram isoladas das amostras coletadas. Dentre as cepas isoladas, algumas apresentaram resistência a até três antimicrobianos. O presente estudo trouxe um maior conhecimento sobre as enterobactérias presentes no Gavião-carijó (Rupornis magnirostris), mostrando um percentual significativo de enterobactérias de importância na saúde pública, evidenciando também a ocorrência de cepas com perfil de resistência a agentes antimicrobianos.

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Disinfectant and Antimicrobial Susceptibility Profiles of the Big Six Non-O157 Shiga Toxin–Producing Escherichia coli Strains from Food Animals and Humans

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-600 ◽

2016 ◽

Vol 79 (8) ◽

pp. 1355-1370 ◽

Cited By ~ 9

Author(s):

ROSS C. BEIER ◽

EELCO FRANZ ◽

JAMES L. BONO ◽

ROBERT E. MANDRELL ◽

PINA M. FRATAMICO ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Susceptibility ◽

Shiga Toxin ◽

Human Exposure ◽

Antimicrobial Agents ◽

Active Components ◽

Food Animals ◽

Low Prevalence ◽

Susceptibility Profiles ◽

Animal Strains

ABSTRACT The disinfectant and antimicrobial susceptibility profiles of 138 non-O157 Shiga toxin–producing Escherichia coli strains (STECs) from food animals and humans were determined. Antimicrobial resistance (AMR) was moderate (39.1% of strains) in response to 15 antimicrobial agents. Animal strains had a lower AMR prevalence (35.6%) than did human strains (43.9%) but a higher prevalence of the resistance profile GEN-KAN-TET. A decreasing prevalence of AMR was found among animal strains from serogroups O45 > O145 > O121 > O111 > O26 > O103 and among human strains from serogroups O145 > O103 > O26 > O111 > O121 > O45. One animal strain from serogroups O121 and O145 and one human strain from serogroup O26 had extensive drug resistance. A high prevalence of AMR in animal O45 and O121 strains and no resistance or a low prevalence of resistance in human strains from these serogroups suggests a source other than food animals for human exposure to these strains. Among the 24 disinfectants evaluated, all strains were susceptible to triclosan. Animal strains had a higher prevalence of resistance to chlorhexidine than did human strains. Both animal and human strains had a similar low prevalence of low-level benzalkonium chloride resistance, and animal and human strains had similar susceptibility profiles for most other disinfectants. Benzyldimethylammonium chlorides and C10AC were the primary active components in disinfectants DC&R and P-128, respectively, against non-O157 STECs. A disinfectant FS512 MIC ≥ 8 μg/ml was more prevalent among animal O121 strains (61.5%) than among human O121 strains (25%), which may also suggest a source of human exposure to STEC O121 other than food animals. Bacterial inhibition was not dependent solely on pH but was correlated with the presence of dissociated organic acid species and some undissociated acids.

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Biofilms and Antimicrobial Susceptibility Profiles of Escherichia coli Recovered from Wastewater Treatment Plants in Kakamega Municipality, Kenya

Egyptian Journal of Microbiology ◽

10.21608/ejm.2021.41683.1171 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Evans Anubi Boge ◽

Peter Nyongesa ◽

Patrick Okoth ◽

Anthony Wawire Sifuna

Keyword(s):

Escherichia Coli ◽

Wastewater Treatment ◽

Antimicrobial Susceptibility ◽

Wastewater Treatment Plants ◽

Susceptibility Profiles

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Antimicrobial Susceptibility Pattern and ESBL Prevalence of Bacteria Isolated from Street Vended Snacks

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i1230415 ◽

2021 ◽

pp. 74-80

Author(s):

Chidiebere Anab-Atulomah ◽

Ngozi C. Oji ◽

Vivian N. Kanu

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Antimicrobial Susceptibility ◽

Salmonella Typhi ◽

Diffusion Method ◽

Susceptibility Pattern ◽

Laboratory Unit ◽

Antimicrobial Susceptibility Pattern ◽

Significant Difference ◽

Esbl Producers

Aims: The objective of the study was to ascertain the antimicrobial susceptibility pattern and ESBL prevalence of bacteria isolated from snacks. Place and Duration of Study: Department of Microbiology (Laboratory Unit) Michael Okpara University of Agriculture Umudike. Methodology: The snacks were mashed aseptically, serially diluted and inoculated onto nutrient agar and MacConkey agar. Isolates were identified using standard microbiological procedures. Antimicrobial susceptibility of the isolates and ESBL detection was done using disk diffusion method. ESBL production was confirmed using Double Disc Synergy Test (DDST) method following CLSI recommendations. Results: Escherichia coli, Salmonella Typhi, Staphylococcus aureus and Klebsiella pneumoniae were the bacteria isolated with Escherichia coli as the most prevalent isolate with 42% occurrence in the samples screened. There was significant difference in the sensitivity of the bacteria isolates to the different antibiotics used at P=0.05. Salmonella Typhi isolates exhibited highest resistance to an antibiotic with 86% resistance to ciprofloxacin while Klebsiella pneumoniae isolates exhibited the lowest resistance to an antibiotic with 10% resistance to cefotaxime. Among the Gram-negative bacteria, 36% of suspected ESBL producing E. coli isolates were confirmed as ESBL producers indicating the highest occurrence. Conclusion: The study confirmed the presence of bacteria in street vended snacks which exhibited high resistance to antibiotics that could be attributed to the presence of ESBL producers among the isolates.

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