scholarly journals Some coagulase negative Staphylococcus spp. isolated from buffalo can be misidentified as Staphylococcus aureus by phenotypic and Sa442 PCR methods

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Camila C. de Almeida ◽  
Lucas J. L. Pizauro ◽  
Glenn A. Soltes ◽  
Durda Slavic ◽  
Fernando A. de Ávila ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Coagulase Negative Staphylococcus ◽  
Staphylococcus Spp

scholarly journals A bacteriological study of septicemia in Hilla city

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Rana S. Al-Taweel
Keyword(s):  
Staphylococcus Aureus ◽  
Immunosuppressive Treatment ◽  
Coagulase Negative Staphylococcus ◽  
Bacteriological Study ◽  
E Coli ◽  
Causative Agents ◽  
Life Threatening ◽  
Antibiotics Sensitivity ◽  
Intravascular Devices ◽  
Staphylococcus Spp

Septicemia is a serious bloodstream infection; it can quickly become life-threatening.  The current study aimed to isolate and identify the causative agents of septicemia cases with reference to the antibiogram test.  52 blood samples were collected from a number of incomings and those who lie in Hilla general hospital. After incubation and culturing on suitable media, 30 samples gave growth of one or two species of bacteria. A total of 33 bacterial isolates were obtained, most of them (16 isolates) were belong to coagulase-negative Staphylococcus spp., followed by E. coli (6 isolates), Pseudomonas sp. (5 isolates), Streptococcus sp. (2 isolates), and one isolate for each of Staphylococcus aureus,  Streptococcus pneumonia, Acinetobacter sp., and Listeria monocytogenes. When antibiotics sensitivity test was accomplished, most Staphylococcus spp. were sensitive for daptomycin and had high resistance to both of vancomycin and clindamycin, whereas Staphylococcus aureus was sensitive for most the used antibiotics. Half of E. coli isolates were sensitive, while the second half were resistant to the used antibiotics. Imipenem inhibited the growth of all Pseudomonas isolates, whereas 80% of them were resistant to amikacin. The epidemiology of bacteremia is altering with the aging of the population, shifts in healthcare, and progress in medicine, such as increased use of immunosuppressive treatment, intravascular devices, and invasive procedures.

scholarly journals Comparison of Susceptibility Testing Methods withmecA Gene Analysis for Determining Oxacillin (Methicillin) Resistance in Clinical Isolates of Staphylococcus aureus and Coagulase-Negative Staphylococcus spp.

1999 ◽  
Vol 37 (9) ◽  
pp. 2952-2961 ◽  
Author(s):  
P. Kohner ◽  
J. Uhl ◽  
C. Kolbert ◽  
D. Persing ◽  
F. Cockerill
Keyword(s):  
Staphylococcus Aureus ◽  
Susceptibility Testing ◽  
Methicillin Resistance ◽  
Ambient Air ◽  
Coagulase Negative Staphylococcus ◽  
Testing Methods ◽  
Oxacillin Resistance ◽  
Mueller Hinton ◽  
Agar Dilution ◽  
Staphylococcus Spp

Ninety-nine clinical staphylococcal isolates (58 coagulase-negativeStaphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 or 35°C in ambient air for 24 or 48 h; disk diffusion by using commercially prepared MH medium (Difco) and MH II agar (BBL) and incubation at 35°C in ambient air for 24 or 48 h; and agar screen (spot or swab inoculation) by using commercially prepared agar (Remel) or MH agar (Difco) prepared in-house, each containing 4% NaCl and 6 μg of oxacillin/ml (0.6-μg/ml oxacillin was also studied with MH agar prepared in-house for the agar swab method and CoNS isolates) and incubation at 35°C in ambient air for 24 or 48 h for swab inoculation and at 30 or 35°C in ambient air for 24 or 48 h for spot inoculation. The results for these methods were compared to the results for mecA gene detection by a PCR method. Given the ability to support growth and the results for susceptibility testing (the breakpoint for susceptible isolates was ≤2 μg/ml), the best methods for CoNS isolates were (i) agar dilution by using MH medium supplemented with 4% NaCl and incubation at 35°C for 48 h (no growth failures were noted, and sensitivity was 97.6%) and (ii) agar screen (swab inoculation) by using MH medium prepared in-house supplemented with 4% NaCl and containing 0.6 μg oxacillin/ml and incubation at 35°C for 48 h (one isolate that did not carry the mecA gene did not grow, and the sensitivity was 100%). All but one (agar dilution without added NaCl and incubation at 30°C for 48 h) of the methods tested revealed all oxacillin-resistant S. aureus isolates, and no growth failures occurred with any method. If the breakpoint for susceptibility was lowered to ≤1 μg/ml for agar dilution methods, more CoNS isolates with oxacillin resistance related to the mecA gene were detected when 0 or 2% NaCl agar supplementation was used. Only one CoNS isolate with mecA gene-associated resistance was not detected by using agar dilution and MH medium supplemented with 4% NaCl with incubation for 48 h. When the breakpoint for susceptibility was decreased 10-fold (from 6.0 to 0.6 μg of oxacillin per ml) for the agar swab screen method, fully 100% of the CoNS isolates that carried the mecA gene were identified.

scholarly journals Antibacterial activity of propolis-based toothpastes for endodontic treatment

2009 ◽  
Vol 45 (4) ◽  
pp. 795-800 ◽  
Author(s):  
Fausto Rodrigo Victorino ◽  
Clovis Monteiro Bramante ◽  
Evandro Watanabe ◽  
Izabel Yoko Ito ◽  
Selma Luci Franco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Streptococcus Mutans ◽  
Staphylococcus Epidermidis ◽  
Culture Medium ◽  
Endodontic Treatment ◽  
Coagulase Negative Staphylococcus ◽  
Staphylococcus Spp ◽  
Coagulase Positive Staphylococcus

This study evaluated the antibacterial activity of propolis-based toothpastes used as intracanal medication in endodontic treatment. The propolis-based toothpastes were prepared using an extract established in previous studies (identified as A70D and D70D). Calcium hydroxide paste was used as a control. The bacteria employed were Streptococcus mutans (ATCC 25175), Staphylococcus aureus (ATCC 6538), Staphylococcus aureus (ATCC 25923), Kocuria rhizophila (ATCC 9341), Escherichia coli (ATCC 10538), Pseudomonas aeruginosa (ATCC 27853), Enterococcus hirae (ATCC 10541), Streptococcus mutans (ATCC 25175). Five field strains isolated from saliva were used: Staphylococcus spp. (23.1 - coagulase positive), Staphylococcus spp. (23.5 - coagulase negative), Staphylococcus spp. (26.1 - coagulase positive), Staphylococcus spp. (26.5 - coagulase negative) and Staphylococcus epidermidis (6epi). The diffusion-well method on double-layer agar was used in a culture medium of Tryptic Soy Agar. The plates were kept at room temperature for two hours to allow the diffusion of pastes in the culture medium, and then incubated at 35º C for twenty-four hours in aerobiosis and in microaerophilia (S. mutans). After this period, the total diameter of the inhibition halo was measured. The results were analyzed by ANOVA analysis of variance, followed by the Tukey test at p<0.05. The propolis-based toothpastes presented antibacterial activity against 83.3% of the analyzed bacteria. For 66.7% of these bacteria, the propolis-based toothpastes exhibited greater antibacterial activity than calcium hydroxide. The present results allow us to conclude that the experimental pastes A70D and D70D showed good activity against aerobic bacteria, proving more effective than calcium hydroxide.

scholarly journals Study of Conjunctival Microbial Flora in Patients of Intensive Care Unit

2021 ◽  
Vol 35 (4) ◽  
pp. 318-324
Author(s):  
Kadambari Ramani ◽  
Subashini Kaliaperumal ◽  
Sandip Sarkar ◽  
Sujatha Sistla
Keyword(s):  
Intensive Care Unit ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Intensive Care ◽  
Intensive Care Units ◽  
Microbial Flora ◽  
Coagulase Negative Staphylococcus ◽  
Antimicrobial Sensitivity ◽  
Prophylactic Measures ◽  
Staphylococcus Spp

Purpose: The objective of the study was to evaluate the type of conjunctival microbial flora in intensive care unit patients and their antimicrobial sensitivity pattern.Methods: A total of 272 samples (conjunctival swabs) were taken from patients in various intensive care units and sent for culture and sensitivity. An ocular examination was done to look for lagophthalmos, conjunctival discharge, exposure keratitis, and corneal perforation.Results: Majority (82.1%) of the samples showed at least one microbial isolate while 29 (10.7%) samples showed multiple microbial growth. The most common microbes were coagulase negative Staphylococcus spp. (41.5% of isolates), diphtheroids (11.0% of isolates), and Staphylococcus aureus (9.6% of isolates) which are the usual commensals of the ocular surface. Of the other microbes isolated, Pseudomonas aeruginosa (4.0%) was the most common. Eighty-four percent isolates of coagulase negative Staphylococcus sp., 81.8% isolates of diphtheroids and 100% isolates of Staphylococcus aureus were penicillin resistant. All isolates of Enterococcus fecalis were sensitive only to vancomycin. Two hundred and twenty eyes (80.9%) had varying degrees of lagophthalmos. Nineteen (7.0%) had severe corneal exposure changes leading to infectious corneal ulcer and perforation in all of them.Conclusions: The isolates in patients of intensive care units were no different from the normal conjunctival flora though few pathogenic organisms such as Pseudomonas aeruginosa and Acinetobacter sp. were also isolated. Most of the isolates were penicillin resistant. This knowledge will help take appropriate prophylactic measures to contain ocular infections in the intensive care units.

scholarly journals A Study of Microbial Colonisation of Orthopaedic Tourniquets

2009 ◽  
Vol 91 (2) ◽  
pp. 131-134 ◽  
Author(s):  
SMY Ahmed ◽  
R Ahmad ◽  
R Case ◽  
RF Spencer
Keyword(s):  
Staphylococcus Aureus ◽  
Orthopaedic Surgery ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Cross Infection ◽  
Coagulase Negative Staphylococcus ◽  
Standard Protocol ◽  
Methicillin Resistant ◽  
Pseudomonas Spp ◽  
Staphylococcus Spp

INTRODUCTION Tourniquets are employed widely in orthopaedic surgery. The use of the same tourniquet on a repetitive basis without a standard protocol for cleaning may be a source of cross-infection. This study examines the contamination of the tourniquets in our institution. MATERIALS AND METHODS Agar plates were used to take samples from 20 tourniquets employed in orthopaedic procedures. Four sites on each tourniquet were cultured and incubated at 37°C for 48 h. RESULTS All sampled tourniquets were contaminated with colony counts varying from 9 to > 385. Coagulase-negative Staphylococcus spp. were the most commonly grown organisms from the tourniquets (96%).Some tourniquets had growths of important pathogens including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas spp., and S. aureus. On cleaning five tourniquets with Clinell (detergent and disinfectant) wipes (GAMA Healthcare Ltd, London, UK), there was a 99.2% reduction in contamination of the tourniquets 5 min after cleaning. CONCLUSIONS In addition to the manufacturers' guidelines, we recommend the cleaning of tourniquets with a disinfectant wipe before every case.

scholarly journals Coagulase-positive methicillin-resistant Staphylococcus aureus circulating in clinical mastitic goats in Bangladesh

2020 ◽  
Vol 13 (7) ◽  
pp. 1303-1310
Author(s):  
Eaftekhar Ahmed Rana ◽  
Tridip Das ◽  
Avijit Dutta ◽  
Mizanur Rahman ◽  
Mohammad Bayazid Bostami ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Meca Gene ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococcus ◽  
Clinical Parameters ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Lactating Goats ◽  
Staphylococcus Spp

Background and Aim: Staphylococcus aureus is argued as one of the principal organisms responsible for mammary gland infection in lactating goats, causing both clinical and subclinical mastitis. Being highly zoonotic potential, pathogen emergence of methicillin-resistant S. aureus (MRSA) has a significant clinical impact on treatment and management of clinical mastitis. We conducted a cross-sectional study to investigate the prevalence of coagulase-positive S. aureus (CoPS), antimicrobial resistance profile of Staphylococcus spp., prevalence of MRSA, and association between different clinical parameters with CoPS. Materials and Methods: A total of 67 clinical mastitic goats were sampled based on clinical examination and California mastitis test. Standard bacteriological methods were performed to isolate and identify Staphylococcus spp. CoPS were confirmed by nuc gene using polymerase chain reaction (PCR). All staphylococcal isolates were further examined for antimicrobial susceptibility testing by disk diffusion method. MRSA was confirmed based on mecA gene-based PCR. Results: Here, 49 (73.13%; 95% confidence interval [CI], 61.41-82.35) samples were positive for Staphylococcus spp., of which 17 (34.69%; 95% CI, 22.88-48.73) isolates were CoPS and rest of the isolates (32; 65.30%; 95% CI, 51.27-77.12) were identified as coagulase-negative Staphylococcus spp. (coagulase-negative staphylococci [CNS]). Both, CoPS and CNS isolates displayed the highest resistance against tetracycline (76.47% and 75%, respectively) and oxacillin (70.58% and 68.75%, respectively). Notably, all staphylococcal isolates were multidrug-resistant (showed resistance to ≥3 classes of antimicrobials). mecA gene was found in 6 (8.96%; 95% CI, 3.84-18.52) CoPS isolates indicating MRSA strains. Among different clinical parameters, presence of high body temperature (p<0.05), firm udder consistency (p<0.01), bloodstained milk (p<0.00), and pus in milk (p<0.00) were significantly associated with the presence of CoPS in mastitic caprine milk. Conclusion: To the best of our knowledge, this is the first report of MRSA isolated from clinical caprine mastitis cases in Bangladesh. The findings of this study would help in cautious selection as well as administration of antimicrobials for therapeutic management of mastitic goats.

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