scholarly journals The impact of different suturing techniques for abdominal fascia closure on the Interleukin-6 expressions in Rattus norvegicus

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Heryu Prima ◽  
Imam Sofii ◽  
Aditya Rifqi Fauzi ◽  
Ishandono Dachlan ◽  
Gunadi
Keyword(s):  
Incisional Hernia ◽  
Important Determinant ◽  
Healing Process ◽  
Fascial Closure ◽  
Wound Healing Process ◽  
Midline Laparotomy ◽  
Abdominal Fascia ◽  
Suturing Techniques ◽  
The Impact ◽  
Fascia Closure

Abstract Objective Incisional hernia is a frequent complication of midline laparotomy. The suturing technique is an important determinant of the risk of developing an incisional hernia. Moreover, IL-6 has crucial roles in the wound-healing process. We aimed to compare the large stitch vs. small stitch technique for abdominal fascial closure on IL-6 expressions in rats. Results Twenty rats were used. The small stitch group received small tissue bites of 5 mm and the large stitch group received large bites of 10 mm. The incisions of fascia were closed by running sutures. Animals were euthanized on days 4 and 7. Histological sections of the tissue-embedded sutures were analyzed for IL-6 expressions. Two-way ANOVA showed that rats in the small stitch group had similar IL-6 expressions on days 4 and 7 to those in the large stitch group (p = 0.36). In conclusion, the IL-6 expressions are similar between the small and the large stitch groups, implying that different suturing techniques might not have an impact on the incisional hernia occurrence.

scholarly journals P149 THE IMPACT OF COVID-19 ON TACKOMESH RANDOMISED CONTROLLED TRIAL COMPARING SPIRAL-TACK MESH-FIXATION DEVICES IN LAPAROSCOPIC INCISIONAL HERNIA REPAIR USING THE MANCHESTER TECHNIQUE

2021 ◽  
Vol 108 (Supplement_8) ◽  
Author(s):  
James Pilkington ◽  
Catherine Fullwood ◽  
Aali Sheen
Keyword(s):  
Incisional Hernia ◽  
Hernia Repair ◽  
Controlled Trial ◽  
Mesh Fixation ◽  
Operating Time ◽  
Incisional Hernia Repair ◽  
Fascial Closure ◽  
Fixation Time ◽  
Laparoscopic Incisional Hernia Repair ◽  
The Impact

Abstract Aim Provide a report on all patients who underwent laparoscopic incisional hernia repair as part of the TACKoMesh RCT prior to unblinding of treatment arms. Material and Methods Trial recruitment was for primary incisional hernia with a defect diameter of 3–10cm. 63 patients (target 74-136) were operated on prior to the outbreak of COVID-19. Post-operative pain is the primary trial outcome. Surgery was performed with either spiral-tack mesh-fixation device (Protack (permanent) or Reliatack (absorbable)), Symbotex IPOM mesh, and fascial closure with no 1 Maxon suture(s) using extracorporeal knot ties – the Manchester Technique. Data was collected on trial forms and lifestyle questionnaires (SF-36 and CCS). All data were explored and described in RStudio v1.4.1106. Results Patients were aged 36-80 and 36(57.1%) patients were male. Mean preoperative BMI was 30.91(sd5.11,range21.15–43.93). Mean operating time was 80.81(37.34,20-240)minutes. In 13(20.6%) patients multiple hernia defects were identified. A good degree of fascial closure was achieved in all patients using a median of 3(IQR 2.0-3.5)knots. Median mesh-fixation time was 286(159.5-428.0)seconds and a mean 25.24(5.49,14-41)tacks/patient were used. Median length of hospital stay was 3.5(2.0–6.0)days. Patients were asked “Please indicate on this scale [VAS 0–10] the pain that you currently experience from your incisional hernia during activity?”. Median responses for Day0/pre-op, Day1, Day6, Day30 and Day365 were 4.5, 8.0, 6.0, 3.0 and 1.5 respectively. At one year, 7(11%) patients had experienced hernia recurrence and 33(52%) post-operative seroma. Conclusions Target recruitment was not possible owing to COVID-19. The Manchester Technique has comparable recurrence rates. Reported pain increases post-operatively but is reduced at post-operative day30 and day365.

scholarly journals Perineal Wound Identification With Maternity Cool Gel Pad (MCGP) Care Interventions

2021 ◽  
Vol 8 (4) ◽  
pp. 279
Author(s):  
Bina Melvia Girsang ◽  
Eqlima Elfira ◽  
Farida Linda Sari Siregar
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Perineal Wound ◽  
Wound Healing Process ◽  
Post Intervention ◽  
Short Period ◽  
Perineal Wounds ◽  
Postpartum Mothers ◽  
The Impact

<em>Postpartum mothers with an indication of episiotomy will experience a higher level of pain. This birth canal trauma is acute and is expected to recover in a short period of time, can be measured, and without serious complications. The aim of this study was to identify the healing process of postpartum maternal perineal wounds. The intervention was carried out on 31 postpartum mothers with the selection using purposive sampling technique. Maternity cool gel pad (MCGP) which was applied to the perineal wound care intervention on the 2nd and 3rd day after delivery showed the wound healing process was observed using the REEDA measuring instrument and analyzed using the T one sample test. The repair of the wound repair scale from the mean REEDA score (10.81 ± 2.98) occurred in all wounds of the study respondents at post intervention (5.32 ± 1.73). Maternity cool gel pad intervention assisted the wound healing process in post intervention data (P &lt;0.005). The results of this study can be indicative of an inflammatory response locally in perineal wounds, but further research is needed to observe the impact of perineal wound healing with a combination of methods that can help evaluate the perineal wound repair process that can be done by mothers independently at home.</em>

scholarly journals A clinical study of continuous and interrupted fascial closure in emergency midline laparotomy at a tertiary care centre

2017 ◽  
Vol 4 (6) ◽  
pp. 2014
Author(s):  
Rahul D. Kunju ◽  
Vinayak Thakkannavar ◽  
Shrivathsa Merta K. ◽  
Sachin H. G. ◽  
Allen Netto ◽  
...  
Keyword(s):  
Incisional Hernia ◽  
Wound Infection ◽  
Wound Closure ◽  
Tertiary Care ◽  
Fascial Closure ◽  
Emergency Laparotomy ◽  
Closure Technique ◽  
Burst Abdomen ◽  
Midline Laparotomy ◽  
Laparotomy Wound

Background: Commonest approach in emergency open abdominal surgeries remains to be midline laparotomy because it is simple, saves time and causes minimal blood loss. Optimal technique for laparotomy wound closure has been a topic of debate since long. Risk factors for development of incisional hernia and burst abdomen are wound infection, systemic illnesses of patient and closure technique. Factors related to patients like age, gender, body mass index (BMI), systemic illnesses are not modifiable when an emergency laparotomy is the only option. Hence closure technique is one factor where surgeon has total control, which can bring down the incidence of burst abdomen and incisional hernias.Methods: Prospective study conducted in 150 patients who underwent emergency midline laparotomy from December 2014 to February 2016 in Krishna Rajendra Hospital attached to Mysore Medical College and Research Institute, Mysore, Karnataka, India with 6 months’ follow-up after surgery.Results: Most of patients in the study belonged to 30-40-year group and were males (78%). Gastrointestinal perforation peritonitis (52%) was the single most common indication for emergency midline laparotomy. In the continuous and interrupted groups, post-operative wound infection was found in 54.6% and 34.6%, wound dehiscence was found in 16% and 6.6% and incisional hernia in 14.4% and 4% respectively.Conclusions: Interrupted suturing is superior to continuous technique in emergency midline laparotomy wound closure in terms of complications and post-operative morbidity.

scholarly journals Stromal vascular fraction promotes migration of fibroblasts and angiogenesis through regulation of extracellular matrix in the skin wound healing process

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Hongsen Bi ◽  
Hui Li ◽  
Chen Zhang ◽  
Yiqing Mao ◽  
Fangfei Nie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Molecular Mechanisms ◽  
Umbilical Vein ◽  
Healing Process ◽  
Stromal Vascular Fraction ◽  
Wound Healing Process ◽  
Matrix Remodeling ◽  
Skin Defect ◽  
The Impact

Abstract Background A refractory wound is a typical complication of diabetes and is a common outcome after surgery. Current approaches have difficulty in improving wound healing. Recently, non-expanded stromal vascular fraction (SVF), which is derived from mature fat, has opened up new directions for the treatment of refractory wound healing. The aim of the current study is to systematically investigate the impact of SVF on wound healing, including the rate and characteristics of wound healing, ability of fibroblasts to migrate, and blood transport reconstruction, with a special emphasis on their precise molecular mechanisms. Methods SVF was isolated by digestion, followed by filtration and centrifugation, and then validated by immunocytochemistry, a MTS proliferation assay and multilineage potential analysis. A wound model was generated by creating 6-mm-diameter wounds, which include a full skin defect, on the backs of streptozocin-induced hyperglycemic mice. SVF or human adipose-derived stem cell (hADSC) suspensions were subcutaneously injected, and the wounds were characterized over a 9-day period by photography and measurements. A scratch test was used to determine whether changes in the migratory ability of fibroblasts occurred after co-culture with hADSCs. Angiogenesis was observed with human umbilical vein endothelial cells. mRNA from fibroblasts, endotheliocyte, and skin tissue were sequenced by high-throughput RNAseq, and differentially expressed genes, and pathways, potentially regulated by SVF or hADSCs were bioinformatically analyzed. Results Our data show that hADSCs have multiple characteristics of MSC. SVF and hADSCs significantly improved wound healing in hyperglycemic mice. hADSCs improve the migratory ability of fibroblasts and capillary structure formation in HUVECs. SVF promotes wound healing by focusing on angiogenesis and matrix remodeling. Conclusions Both SVF and hADSCs improve the function of fibroblast and endothelial cells, regulate gene expression, and promote skin healing. Various mechanisms likely are involved, including migration of fibroblasts, tubulogenesis of endothelial cells through regulation of cell adhesion, and cytokine pathways.

scholarly journals The Impact of Composites with Silicate-Based Glasses and Gold Nanoparticles on Skin Wound Regeneration

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 620
Author(s):  
Sorin M. Mârza ◽  
Klara Magyari ◽  
Sidonia Bogdan ◽  
Mirela Moldovan ◽  
Cosmin Peștean ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Silver Content ◽  
Histopathological Examination ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Regeneration Process ◽  
Wound Healing Process ◽  
Gold Nanocages ◽  
Skin Healing ◽  
The Impact

The silver content of the skin regeneration ointments can influence its regeneration process but in the meantime, it can take the benefit of the antibacterial properties of silver by avoiding the bacterial infection of an open wound. In the current study, the skin healing and regeneration capacity of bioactive glass with spherical gold nanocages (BGAuIND) in the Vaseline ointments were evaluated in vivo comparing the bioactive glass (BG)-Vaseline and bioactive glass with spherical gold (BGAuSP)-Vaseline ointments. Spherical gold nanocages are stabilized with silver and as a consequence the BGAuIND exhibits great antibacterial activity. Histological examination of the cutaneous tissue performed on day 8 indicates a more advanced regeneration process in rats treated with BGAuSP-Vaseline. The histopathological examination also confirms the results obtained after 11 days post-intervention, when the skin is completely regenerated at rats treated with BGAuSP-Vaseline compared with the others groups where the healing was incomplete. This result is also confirmed by the macroscopic images of the evolution of wounds healing. As expected, the silver content influences the wound healing process but after two weeks, for all of the post-interventional trials from the groups of rats, the skin healing was completely.

scholarly journals The Impact of Stress on Pressure Ulcer Wound Healing Process and on the Psychophysiological Environment of the Individual Suffering from them

2018 ◽  
Vol 72 (3) ◽  
pp. 362 ◽  
Author(s):  
Charalambos Charalambous ◽  
Aristides Vassilopoulos ◽  
Agoritsa Koulouri ◽  
Siamaga Eleni ◽  
Sotiropoulou Popi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Pressure Ulcer ◽  
Healing Process ◽  
Wound Healing Process ◽  
The Individual ◽  
The Impact

scholarly journals Abdominal incision defect following AAA-surgery (AIDA): 2-year results of prophylactic onlay-mesh augmentation in a multicentre, double-blind, randomised controlled trial

2021 ◽  
Author(s):  
S. Honig ◽  
H. Diener ◽  
T. Kölbel ◽  
W. Reinpold ◽  
A. Zapf ◽  
...  
Keyword(s):  
Incisional Hernia ◽  
Controlled Trial ◽  
Fascial Closure ◽  
Absorbable Suture ◽  
Double Blind ◽  
Midline Laparotomy ◽  
Mesh Augmentation ◽  
Onlay Mesh ◽  
Randomised Controlled

AbstractThe reported incidence of incisional hernia following repair of abdominal aortic aneurysm (AAA) via midline laparotomy is up to 69%. This prospective, multicenter, double-blind, randomised controlled trial was conducted at eleven hospitals in Germany. Patients aged 18 years or older undergoing elective AAA-repair via midline incision were randomly assigned using a computer-generated randomisation sequence to one of three groups for fascial closure: with long-term absorbable suture (MonoPlus®, group I), long-term absorbable suture and onlay mesh reinforcement (group II) or extra long-term absorbable suture (MonoMax®, group III). The primary endpoint was the incidence of incisional hernia within 24 months of follow-up, analysed by intention to treat. Physicians conducting the postoperative visits and the patients were blinded. Between February 2011 and July 2013, 104 patients (69.8 ± 7.7 years) were randomised, 99 of them received a study intervention. The rate of incisional hernia within 24 months was not significantly reduced with onlay mesh augmentation compared to primary suture (p = 0.290). Furthermore, the rate of incisional hernia did not differ significantly between fascial closure with slow and extra long-term absorbable suture (p = 0.111). Serious adverse events related to study intervention occurred in five patients (5.1%) from treatment groups II and III. Wound healing disorders were more frequently seen after onlay mesh implantation on the day of discharge (p = 0.010) and three (p = 0.009) and six (p = 0.023) months postoperatively. The existing evidence on prophylactic mesh augmentation in patients undergoing AAA-repair via midline laparotomy probably needs critical review. As the implementation of new RCTs is considered difficult due to the increasing number of endovascular AAA treated, registry studies could help to collect and evaluate data in cases of open AAA-repair. Comparisons between prophylactic mesh implantation and the small bite technique are also required. Trial registration: ClinicalTrials.gov Identifier: NCT01353443. Funding Sources: Aesculap AG, Tuttlingen, Germany.

scholarly journals Green Extraction of Carotenoids from Fruit and Vegetable Byproducts: A Review

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 518
Author(s):  
Ewelina Kultys ◽  
Marcin Andrzej Kurek
Keyword(s):  
Animal Feed ◽  
Healing Process ◽  
Process Efficiency ◽  
Wound Healing Process ◽  
Fruit And Vegetable ◽  
Bioactive Substances ◽  
Green Extraction ◽  
Wide Range ◽  
Fruit Processing ◽  
The Impact

Carotenoids are characterized by a wide range of health-promoting properties. For example, they support the immune system and wound healing process and protect against UV radiation’s harmful effects. Therefore, they are used in the food industry and cosmetics, animal feed, and pharmaceuticals. The main sources of carotenoids are the edible and non-edible parts of fruit and vegetables. Therefore, the extraction of bioactive substances from the by-products of vegetable and fruit processing can greatly reduce food waste. This article describes the latest methods for the extraction of carotenoids from fruit and vegetable byproducts, such as solvent-free extraction—which avoids the costs and risks associated with the use of petrochemical solvents, reduces the impact on the external environment, and additionally increases the purity of the extract—or green extraction using ultrasound and microwaves, which enables a significant improvement in process efficiency and reduction in extraction time. Another method is supercritical extraction with CO2, an ideal supercritical fluid that is non-toxic, inexpensive, readily available, and easily removable from the product, with a high penetration capacity.

scholarly journals The Composition of Surgical Wound Fluids from Breast Cancer Patients is Affected by Intraoperative Radiotherapy Treatment and Depends on the Molecular Subtype of Breast Cancer

Cancers ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 11 ◽  
Author(s):  
Katarzyna Kulcenty ◽  
Igor Piotrowski ◽  
Joanna Patrycja Wróblewska ◽  
Janusz Wasiewicz ◽  
Wiktoria Maria Suchorska
Keyword(s):  
Breast Cancer ◽  
Wound Healing ◽  
Molecular Subtype ◽  
Breast Cancer Subtype ◽  
Healing Process ◽  
Intraoperative Radiotherapy ◽  
Wound Healing Process ◽  
Surgical Wound ◽  
Luminal A ◽  
The Impact

Invasive oncological procedures affect the remaining tumor cells by increasing their survival, proliferation, and migration through the induction of wound healing response. The phenomena of local relapse after breast-conserving surgery (BCS) has resulted in a series of research and clinical trials with the aim of assessing whether localized intraoperative radiotherapy (IORT), may be beneficial in inhibiting local recurrences. Therefore, it is essential to assess the impact of intraoperative radiotherapy in modulating the immunological response and wound healing process. Thus, we decided to perform a quantitative analysis of the composition of surgical wound fluids (SWF) in two groups of breast cancer (BC) patients: those treated with BCS followed by IORT, and those who underwent BCS alone. We found that several cytokines, which are believed to have anti-tumor properties, were highly expressed in the luminal A breast cancer subtype in the IORT treatment group. Interestingly, we also found significant differences between IORT patients with tumors of different molecular subtypes. Based on these findings, we hypothesized that IORT treatment might be beneficial in changing the tumor bed microenvironment, making it less favorable for tumor recurrence due to decreased concentration of tumor-facilitating cytokines, especially in the luminal A subtype of BC.

Hard-to-heal wounds, biofilm and wound healing: an intricate interrelationship

2020 ◽  
Vol 29 (5) ◽  
pp. S6-S13 ◽  
Author(s):  
Maria-Manuel Azevedo ◽  
Carmen Lisboa ◽  
Luís Cobrado ◽  
Cidália Pina-Vaz ◽  
Acácio Rodrigues
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Public Health Problem ◽  
Wound Healing Process ◽  
Major Public Health Problem ◽  
Normal Wound Healing ◽  
Efficient Management ◽  
Acute Wound ◽  
Reparative Process ◽  
The Impact

Hard-to-heal wounds are a major public health problem that incur high economic costs. A major source of morbidity, they can have an overwhelming impact on patients, caregivers and society. In contrast to acute wound healing, which follows an ‘orderly and timely reparative process', the healing of hard-to-heal wounds is delayed because the usual biological progression is interrupted. This article discusses hard-to-heal wounds, the impact they have on patients and healthcare systems, and how biofilms and other factors affect the wound-healing process. Controlling and preventing infection is of utmost importance for normal wound healing. Rational use of anti-infectious agents is crucial and is particularly relevant in the context of rising healthcare costs. Knowledge of the complex relationship between hard-to-heal wounds, biofilm formation and wound healing is vital for efficient management of hard-to-heal wounds.

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